pycom16g13950

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012224
Physical Location & Seq
Forward (+)
10000349 .. 10001004
656 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g13950.2

Sequence Viewer

Length: 552 bp
ATGTCCAACAAGTCACCAATCTTTCCGATGCCACAAACCCAACACTTCAGCGACTATGGCTTCGACCCCCAAATCGACTACTTTCAGGTTCTGGAGGACGCAAGGAGGCACAAGCGGGAGACGACGAGATCCATAGACTCCGTACACTTCAAGCTCCAAAAACCCATCTCAAAAGACGACCCCAAAATGCCCCACCACAAGAAAACCAAGAAGAAGCGCTGGTGGAAAAGCGCTCTGCTTTTCTTCAAATGGAACAAGCTGACAACCCACCACTACCACCGCGGCCCCGCCGGTGATGAAGATGTTCATCAGGCCAGGGCCAAGGCCTTCCGGGCTTCGATTTCTGGGCCGGTTTACATCACTGAGAGCAGAAGCGGGTCGAGCACGCCTTACCGGAGTACTAGCAGGCCGTCGTCGGGACCGCTTGCCGGAACAATGTCTCCGGCGAGTAAAGATGAGATGGGCATCCCCTACCTGAGCCTGAGGGAGCTTAACATGGAGCAGCAACAGAGGAGCTCTACATCTGCCATGCCTATATATTTGGTTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

21.05

Weight (kDa)

9.95

Isoelectric Point (pI)

74.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06840
fragaria_vesca FvH4_4g18620
malus_domestica MD13G1165100.v1.1 MD13G1165200.v1.1 MD16G1164800.v1.1
prunus_persica Prupe.1G124100_v2.0.a1
pyrus_communis pycom13g14120 pycom13g14150 pycom16g13950
rosa_chinensis RchiOBHm_Chr4g0423321
rosa_laevigata RLG00000007569
rosa_multiflora Rmu_co8278673.1_g000001 Rmu_sc0005307.1_g000001
rosa_roxburghii Rroxscaffold_5G00365500
rosa_rugosa Rorug04G0186500
rosa_samantha Rh4AG245700 Rh4BG251100 Rh4CG259500
rosa_wichuraiana Rw4G021110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 282
AciI CCGC 6 cut(s) 115, 280, 282, 288, 375, 422
AclWI GGATC 1 cut(s) 123
AcuI CTGAAG 1 cut(s) 31
AfaI GTAC 2 cut(s) 144, 400
AfeI AGCGCT 2 cut(s) 218, 232
AfiI CCNNNNNNNGG 2 cut(s) 416, 428
AgsI TTSAA 2 cut(s) 151, 247
AjnI CCWGG 1 cut(s) 314
AloI GAACNNNNNNTCC 2 cut(s) 424, 456
AluBI AGCT 4 cut(s) 154, 259, 490, 516
AluI AGCT 4 cut(s) 154, 259, 490, 516
Alw21I GWGCWC 2 cut(s) 386, 518
Alw26I GTCTC 2 cut(s) 113, 444
AlwI GGATC 1 cut(s) 123
AlwNI CAGNNNCTG 1 cut(s) 91
Aor51HI AGCGCT 2 cut(s) 218, 232
AoxI GGCC 6 cut(s) 283, 312, 318, 324, 347, 407
ApeKI GCWGC 1 cut(s) 502
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 1 cut(s) 303
AspLEI GCGC 2 cut(s) 219, 233
AspS9I GGNCC 4 cut(s) 284, 318, 347, 419
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 6, 305
AvaII GGWCC 1 cut(s) 419
AxyI CCTNAGG 1 cut(s) 482
BanII GRGCYC 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 386, 518
BbvI GCAGC 1 cut(s) 514
BccI CCATC 2 cut(s) 173, 454
BceAI ACGGC 1 cut(s) 394
BciT130I CCWGG 1 cut(s) 316
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 2 cut(s) 113, 444
BfaI CTAG 1 cut(s) 402
BfoI RGCGCY 2 cut(s) 220, 234
BglI GCCNNNNNGGC 1 cut(s) 332
BisI GCNGC 2 cut(s) 283, 503
BlsI GCNGC 2 cut(s) 284, 504
BmcAI AGTACT 1 cut(s) 400
Bme1390I CCNGG 2 cut(s) 316, 332
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 4 cut(s) 284, 318, 347, 419
BmiI GGNNCC 2 cut(s) 286, 420
BmrFI CCNGG 2 cut(s) 316, 332
BmsI GCATC 2 cut(s) 18, 474
BpmI CTGGAG 1 cut(s) 113
Bpu10I CCTNAGC 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 332
BsaBI GATNNNNATC 2 cut(s) 306, 464
BsaJI CCNNGG 3 cut(s) 280, 315, 321
BsaWI WCCGGW 1 cut(s) 393
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 2 cut(s) 416, 428
Bse118I RCCGGY 2 cut(s) 290, 349
Bse21I CCTNAGG 1 cut(s) 482
Bse8I GATNNNNATC 2 cut(s) 306, 464
BseBI CCWGG 1 cut(s) 316
BseDI CCNNGG 3 cut(s) 280, 315, 321
BseGI GGATG 1 cut(s) 465
BseJI GATNNNNATC 2 cut(s) 306, 464
BseLI CCNNNNNNNGG 2 cut(s) 416, 428
BseMII CTCAG 3 cut(s) 354, 467, 473
BseRI GAGGAG 1 cut(s) 526
BseXI GCAGC 1 cut(s) 514
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 6 cut(s) 285, 314, 320, 326, 349, 409
BsiHKAI GWGCWC 2 cut(s) 386, 518
BsiSI CCGG 6 cut(s) 291, 331, 350, 394, 429, 443
BslFI GGGAC 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 416, 428
BsmAI GTCTC 2 cut(s) 113, 444
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 432
BsnI GGCC 6 cut(s) 285, 314, 320, 326, 349, 409
Bsp1286I GDGCHC 2 cut(s) 386, 518
Bsp143I GATC 1 cut(s) 128
BspACI CCGC 6 cut(s) 115, 280, 282, 288, 375, 422
BspANI GGCC 6 cut(s) 285, 314, 320, 326, 349, 409
BspCNI CTCAG 3 cut(s) 355, 468, 474
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 286, 420
BspPI GGATC 1 cut(s) 123
BsrFI RCCGGY 2 cut(s) 290, 349
BssAI RCCGGY 2 cut(s) 290, 349
BssECI CCNNGG 3 cut(s) 280, 315, 321
BssMI GATC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 316
BstC8I GCNNGC 3 cut(s) 386, 407, 426
BstDEI CTNAG 3 cut(s) 363, 476, 482
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 2 cut(s) 220, 234
BstHHI GCGC 2 cut(s) 219, 233
BstKTI GATC 1 cut(s) 131
BstMAI GTCTC 2 cut(s) 113, 444
BstMBI GATC 1 cut(s) 128
BstMWI GCNNNNNNNGC 3 cut(s) 57, 332, 381
BstNI CCWGG 1 cut(s) 316
BstSCI CCNGG 2 cut(s) 314, 330
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 514
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
Bsu36I CCTNAGG 1 cut(s) 482
BsuRI GGCC 6 cut(s) 285, 314, 320, 326, 349, 409
BtgI CCRYGG 1 cut(s) 280
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 3 cut(s) 386, 407, 426
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 2 cut(s) 219, 233
Cfr10I RCCGGY 2 cut(s) 290, 349
Cfr13I GGNCC 4 cut(s) 284, 318, 347, 419
Cfr42I CCGCGG 1 cut(s) 283
CseI GACGC 1 cut(s) 107
Csp6I GTAC 2 cut(s) 143, 399
CviAII CATG 2 cut(s) 496, 529
CviQI GTAC 2 cut(s) 143, 399
DdeI CTNAG 3 cut(s) 363, 476, 482
DpnI GATC 1 cut(s) 130
DpnII GATC 1 cut(s) 128
Ecl136II GAGCTC 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 321
Eco147I AGGCCT 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 518
Eco47I GGWCC 1 cut(s) 419
Eco47III AGCGCT 2 cut(s) 218, 232
Eco53kI GAGCTC 1 cut(s) 516
Eco57I CTGAAG 1 cut(s) 31
Eco81I CCTNAGG 1 cut(s) 482
EcoICRI GAGCTC 1 cut(s) 516
EcoRII CCWGG 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 321
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 2 cut(s) 499, 532
FaiI YATR 6 cut(s) 57, 134, 497, 530, 536, 538
FaqI GGGAC 1 cut(s) 432
FatI CATG 2 cut(s) 495, 528
FauI CCCGC 3 cut(s) 108, 295, 368
Fnu4HI GCNGC 2 cut(s) 283, 503
FokI GGATG 1 cut(s) 452
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 2 cut(s) 283, 503
FspBI CTAG 1 cut(s) 402
GlaI GCGC 2 cut(s) 218, 232
GluI GCNGC 2 cut(s) 283, 503
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 220, 234
HaeIII GGCC 6 cut(s) 285, 314, 320, 326, 349, 409
HapII CCGG 6 cut(s) 291, 331, 350, 394, 429, 443
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 219, 233
Hin1II CATG 2 cut(s) 499, 532
Hin6I GCGC 2 cut(s) 217, 231
HinP1I GCGC 2 cut(s) 217, 231
HinfI GANTC 1 cut(s) 137
HpaII CCGG 6 cut(s) 291, 331, 350, 394, 429, 443
HphI GGTGA 2 cut(s) 6, 305
Hpy166II GTNNAC 2 cut(s) 145, 355
Hpy188I TCNGA 1 cut(s) 27
Hpy188III TCNNGA 2 cut(s) 92, 417
Hpy8I GTNNAC 2 cut(s) 145, 355
Hpy99I CGWCG 3 cut(s) 127, 415, 418
HpyAV CCTTC 1 cut(s) 337
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 332, 381
HpyF3I CTNAG 3 cut(s) 363, 476, 482
Hsp92II CATG 2 cut(s) 499, 532
HspAI GCGC 2 cut(s) 217, 231
KspI CCGCGG 1 cut(s) 283
Kzo9I GATC 1 cut(s) 128
LmnI GCTCC 4 cut(s) 159, 487, 499, 513
Lsp1109I GCAGC 1 cut(s) 514
LweI GCATC 2 cut(s) 18, 474
MaeI CTAG 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 12, 544
MalI GATC 1 cut(s) 130
MboI GATC 1 cut(s) 128
MboII GAAGA 3 cut(s) 223, 235, 311
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 2 cut(s) 386, 518
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 4 cut(s) 88, 99, 477, 504
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 2 cut(s) 492, 550
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 6 cut(s) 291, 331, 350, 394, 429, 443
MspR9I CCNGG 2 cut(s) 316, 332
MvaI CCWGG 1 cut(s) 316
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 3 cut(s) 57, 332, 381
NciI CCSGG 1 cut(s) 332
NdeII GATC 1 cut(s) 128
NlaIII CATG 2 cut(s) 499, 532
NlaIV GGNNCC 2 cut(s) 286, 420
NmuCI GTSAC 1 cut(s) 12
PceI AGGCCT 1 cut(s) 326
PdmI GAANNNNTTC 1 cut(s) 303
PkrI GCNGC 2 cut(s) 284, 504
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 314
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 286, 420
PspPI GGNCC 4 cut(s) 284, 318, 347, 419
PstNI CAGNNNCTG 1 cut(s) 91
PsuI RGATCY 1 cut(s) 128
RsaI GTAC 2 cut(s) 144, 400
RsaNI GTAC 2 cut(s) 143, 399
SacI GAGCTC 1 cut(s) 518
SacII CCGCGG 1 cut(s) 283
SaqAI TTAA 2 cut(s) 492, 550
SatI GCNGC 2 cut(s) 283, 503
Sau3AI GATC 1 cut(s) 128
Sau96I GGNCC 4 cut(s) 284, 318, 347, 419
ScaI AGTACT 1 cut(s) 400
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 2 cut(s) 316, 332
SduI GDGCHC 2 cut(s) 386, 518
SetI ASST 6 cut(s) 90, 156, 261, 477, 492, 518
SfaNI GCATC 2 cut(s) 18, 474
Sfr303I CCGCGG 1 cut(s) 283
SgrAI CRCCGGYG 1 cut(s) 290
SgrBI CCGCGG 1 cut(s) 283
SinI GGWCC 1 cut(s) 419
SseBI AGGCCT 1 cut(s) 326
SsiI CCGC 6 cut(s) 115, 280, 282, 288, 375, 422
SspMI CTAG 1 cut(s) 402
SstI GAGCTC 1 cut(s) 518
StuI AGGCCT 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 314, 330
StyI CCWWGG 1 cut(s) 321
TaqI TCGA 4 cut(s) 63, 75, 338, 380
TatI WGTACW 1 cut(s) 398
TauI GCSGC 1 cut(s) 285
Tru1I TTAA 2 cut(s) 492, 550
Tru9I TTAA 2 cut(s) 492, 550
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 12
TseI GCWGC 1 cut(s) 502
Tsp45I GTSAC 1 cut(s) 12
TspDTI ATGAA 2 cut(s) 296, 312
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 1 cut(s) 402
ZrmI AGTACT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.