Rh4BG251100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012224
Physical Location & Seq
Reverse (-)
42081193 .. 42082585
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG251100.1

Sequence Viewer

Length: 564 bp
ATGTCGAACAAGTCACCCATTTTTCCGATGCCGGAGCCTCAACACTTCAGCGACTACGGCTTCGACCCCCAAATCGACTATTTCCAGGTCCTTGAAGAAGCTAGAAGGCACAAGCGTGACGGAACGAGATCCATAGACTCGATTCACTTCAAGCTCCAGAAGCCCATTTCCAAGGACGACCACTACTCCAAGAAGCCTCACAAGAAGAAGCGCTGGTGGAAAAGCGCTTTGCTTTTCTTCAAATGGGGCTGGACCCACCACCACCACCACGACTCGGCCCACCGCCACAACCGCCGTGGTGGCCGGGAGGACGTGCACCAGGCCAGGGCCAAGGTTTTCCGGGCTTCCATTTCCGGGCCGGTTTATATCACAGAAAGCAGGAGCGGGTCGAGCACACCGTACCGGACAGCGAGCCGGCCGTCGTCGGGACCGCTGGCCGGGACTATGTCGCCGGTGAGTAAGGGAGAGATGGAGATTCCGTATCTGAGCCTGAGGGAGCTTAACATGGAGCAGCAACAGAGGGTGTCTACCTCATCAGCCATGCCCATTTACTTGGTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

21.71

Weight (kDa)

10.03

Isoelectric Point (pI)

70.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06840
fragaria_vesca FvH4_4g18620
malus_domestica MD13G1165100.v1.1 MD13G1165200.v1.1 MD16G1164800.v1.1
prunus_persica Prupe.1G124100_v2.0.a1
pyrus_communis pycom13g14120 pycom13g14150 pycom16g13950
rosa_chinensis RchiOBHm_Chr4g0423321
rosa_laevigata RLG00000007569
rosa_multiflora Rmu_co8278673.1_g000001 Rmu_sc0005307.1_g000001
rosa_roxburghii Rroxscaffold_5G00365500
rosa_rugosa Rorug04G0186500
rosa_samantha Rh4AG245700 Rh4BG251100 Rh4CG259500
rosa_wichuraiana Rw4G021110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 384
AccI GTMKAC 1 cut(s) 527
AciI CCGC 4 cut(s) 283, 292, 384, 431
AclWI GGATC 1 cut(s) 123
AcoI YGGCCR 3 cut(s) 301, 416, 435
AcuI CTGAAG 1 cut(s) 31
AfaI GTAC 1 cut(s) 401
AfeI AGCGCT 2 cut(s) 212, 226
AfiI CCNNNNNNNGG 5 cut(s) 274, 325, 354, 425, 437
AgsI TTSAA 3 cut(s) 95, 151, 241
AjiI CACGTC 1 cut(s) 313
AjnI CCWGG 3 cut(s) 84, 318, 323
AleI CACNNNNGTG 1 cut(s) 114
AluBI AGCT 3 cut(s) 101, 154, 499
AluI AGCT 3 cut(s) 101, 154, 499
Alw21I GWGCWC 2 cut(s) 318, 395
Alw44I GTGCAC 1 cut(s) 314
AlwI GGATC 1 cut(s) 123
Aor51HI AGCGCT 2 cut(s) 212, 226
AoxI GGCC 7 cut(s) 276, 301, 321, 327, 356, 416, 435
ApaLI GTGCAC 1 cut(s) 314
ApeKI GCWGC 1 cut(s) 511
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 2 cut(s) 213, 227
AspS9I GGNCC 6 cut(s) 88, 252, 277, 327, 356, 428
AsuC2I CCSGG 4 cut(s) 305, 341, 355, 439
AsuHPI GGTGA 2 cut(s) 6, 466
AvaII GGWCC 3 cut(s) 88, 252, 428
AxyI CCTNAGG 1 cut(s) 491
BaeGI GKGCMC 1 cut(s) 318
Bbv12I GWGCWC 2 cut(s) 318, 395
BbvI GCAGC 1 cut(s) 523
BccI CCATC 1 cut(s) 463
BceAI ACGGC 3 cut(s) 73, 279, 403
BciT130I CCWGG 3 cut(s) 86, 320, 325
BcnI CCSGG 4 cut(s) 305, 341, 355, 439
BfaI CTAG 1 cut(s) 102
BfoI RGCGCY 2 cut(s) 214, 228
BglI GCCNNNNNGGC 1 cut(s) 300
BisI GCNGC 1 cut(s) 512
BlsI GCNGC 1 cut(s) 513
Bme1390I CCNGG 7 cut(s) 86, 305, 320, 325, 341, 355, 439
Bme18I GGWCC 3 cut(s) 88, 252, 428
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 6 cut(s) 88, 252, 277, 327, 356, 428
BmiI GGNNCC 3 cut(s) 36, 254, 429
BmrFI CCNGG 7 cut(s) 86, 305, 320, 325, 341, 355, 439
BmsI GCATC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 140
BpuMI CCSGG 4 cut(s) 305, 341, 355, 439
BsaJI CCNNGG 4 cut(s) 171, 295, 324, 330
BsaWI WCCGGW 1 cut(s) 402
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 464, 494
Bsc4I CCNNNNNNNGG 5 cut(s) 274, 325, 354, 425, 437
Bse118I RCCGGY 3 cut(s) 358, 414, 451
Bse21I CCTNAGG 1 cut(s) 491
BseBI CCWGG 3 cut(s) 86, 320, 325
BseDI CCNNGG 4 cut(s) 171, 295, 324, 330
BseLI CCNNNNNNNGG 5 cut(s) 274, 325, 354, 425, 437
BseMII CTCAG 2 cut(s) 476, 482
BseSI GKGCMC 1 cut(s) 318
BseX3I CGGCCG 1 cut(s) 416
BseXI GCAGC 1 cut(s) 523
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 7 cut(s) 278, 303, 323, 329, 358, 418, 437
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 2 cut(s) 318, 395
BsiSI CCGG 9 cut(s) 32, 304, 340, 354, 359, 403, 415, 438, 452
BslFI GGGAC 2 cut(s) 441, 454
BslI CCNNNNNNNGG 5 cut(s) 274, 325, 354, 425, 437
BsmFI GGGAC 2 cut(s) 441, 454
BsnI GGCC 7 cut(s) 278, 303, 323, 329, 358, 418, 437
Bsp1286I GDGCHC 2 cut(s) 318, 395
Bsp143I GATC 1 cut(s) 128
BspACI CCGC 4 cut(s) 283, 292, 384, 431
BspANI GGCC 7 cut(s) 278, 303, 323, 329, 358, 418, 437
BspCNI CTCAG 2 cut(s) 477, 483
BspLI GGNNCC 3 cut(s) 36, 254, 429
BspPI GGATC 1 cut(s) 123
BsrBI CCGCTC 1 cut(s) 384
BsrFI RCCGGY 3 cut(s) 358, 414, 451
BssAI RCCGGY 3 cut(s) 358, 414, 451
BssECI CCNNGG 4 cut(s) 171, 295, 324, 330
BssMI GATC 1 cut(s) 128
BssT1I CCWWGG 2 cut(s) 171, 330
Bst2UI CCWGG 3 cut(s) 86, 320, 325
Bst4CI ACNGT 1 cut(s) 399
BstC8I GCNNGC 3 cut(s) 412, 416, 435
BstDEI CTNAG 2 cut(s) 485, 491
BstDSI CCRYGG 1 cut(s) 295
BstH2I RGCGCY 2 cut(s) 214, 228
BstHHI GCGC 2 cut(s) 213, 227
BstKTI GATC 1 cut(s) 131
BstMBI GATC 1 cut(s) 128
BstMCI CGRYCG 1 cut(s) 419
BstMWI GCNNNNNNNGC 5 cut(s) 57, 160, 291, 300, 390
BstNI CCWGG 3 cut(s) 86, 320, 325
BstSCI CCNGG 7 cut(s) 84, 303, 318, 323, 339, 353, 437
BstSLI GKGCMC 1 cut(s) 318
BstV1I GCAGC 1 cut(s) 523
BstX2I RGATCY 1 cut(s) 128
BstXI CCANNNNNNTGG 1 cut(s) 553
BstYI RGATCY 1 cut(s) 128
BstZI CGGCCG 1 cut(s) 416
Bsu36I CCTNAGG 1 cut(s) 491
BsuRI GGCC 7 cut(s) 278, 303, 323, 329, 358, 418, 437
BtgI CCRYGG 1 cut(s) 295
BtrI CACGTC 1 cut(s) 313
Cac8I GCNNGC 3 cut(s) 412, 416, 435
CfoI GCGC 2 cut(s) 213, 227
Cfr10I RCCGGY 3 cut(s) 358, 414, 451
Cfr13I GGNCC 6 cut(s) 88, 252, 277, 327, 356, 428
Csp6I GTAC 1 cut(s) 400
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviAII CATG 2 cut(s) 505, 541
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 2 cut(s) 485, 491
DpnI GATC 1 cut(s) 130
DpnII GATC 1 cut(s) 128
EaeI YGGCCR 3 cut(s) 301, 416, 435
EagI CGGCCG 1 cut(s) 416
EclXI CGGCCG 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 171, 330
Eco47I GGWCC 3 cut(s) 88, 252, 428
Eco47III AGCGCT 2 cut(s) 212, 226
Eco52I CGGCCG 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 31
Eco81I CCTNAGG 1 cut(s) 491
EcoO109I RGGNCCY 1 cut(s) 88
EcoRII CCWGG 3 cut(s) 84, 318, 323
EcoT14I CCWWGG 2 cut(s) 171, 330
ErhI CCWWGG 2 cut(s) 171, 330
FaeI CATG 2 cut(s) 508, 544
FaiI YATR 5 cut(s) 134, 366, 446, 506, 542
FaqI GGGAC 2 cut(s) 441, 454
FatI CATG 2 cut(s) 504, 540
FauI CCCGC 1 cut(s) 377
FblI GTMKAC 1 cut(s) 527
Fnu4HI GCNGC 1 cut(s) 512
Fsp4HI GCNGC 1 cut(s) 512
FspBI CTAG 1 cut(s) 102
GlaI GCGC 2 cut(s) 212, 226
GluI GCNGC 1 cut(s) 512
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 2 cut(s) 214, 228
HaeIII GGCC 7 cut(s) 278, 303, 323, 329, 358, 418, 437
HapII CCGG 9 cut(s) 32, 304, 340, 354, 359, 403, 415, 438, 452
HhaI GCGC 2 cut(s) 213, 227
Hin1II CATG 2 cut(s) 508, 544
Hin6I GCGC 2 cut(s) 211, 225
HinP1I GCGC 2 cut(s) 211, 225
HinfI GANTC 4 cut(s) 137, 142, 272, 475
HpaII CCGG 9 cut(s) 32, 304, 340, 354, 359, 403, 415, 438, 452
HphI GGTGA 2 cut(s) 6, 466
Hpy166II GTNNAC 2 cut(s) 316, 528
Hpy188I TCNGA 2 cut(s) 27, 486
Hpy188III TCNNGA 2 cut(s) 157, 426
Hpy8I GTNNAC 2 cut(s) 316, 528
Hpy99I CGWCG 2 cut(s) 424, 427
HpyAV CCTTC 1 cut(s) 99
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 1 cut(s) 316
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 160, 291, 300, 390
HpyF3I CTNAG 2 cut(s) 485, 491
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 2 cut(s) 508, 544
HspAI GCGC 2 cut(s) 211, 225
KroI GCCGGC 1 cut(s) 414
KroNI GCCGGC 1 cut(s) 416
Kzo9I GATC 1 cut(s) 128
LmnI GCTCC 5 cut(s) 34, 159, 381, 496, 508
Lsp1109I GCAGC 1 cut(s) 523
LweI GCATC 1 cut(s) 18
MaeI CTAG 1 cut(s) 102
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 3 cut(s) 12, 116, 556
MalI GATC 1 cut(s) 130
MbiI CCGCTC 1 cut(s) 384
MboI GATC 1 cut(s) 128
MboII GAAGA 3 cut(s) 107, 217, 229
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 2 cut(s) 318, 395
MlyI GAGTC 2 cut(s) 131, 266
MnlI CCTC 6 cut(s) 48, 207, 301, 486, 513, 541
MroNI GCCGGC 1 cut(s) 414
MseI TTAA 1 cut(s) 501
MslI CAYNNNNRTG 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 433
MspI CCGG 9 cut(s) 32, 304, 340, 354, 359, 403, 415, 438, 452
MspR9I CCNGG 7 cut(s) 86, 305, 320, 325, 341, 355, 439
MvaI CCWGG 3 cut(s) 86, 320, 325
MwoI GCNNNNNNNGC 5 cut(s) 57, 160, 291, 300, 390
NaeI GCCGGC 1 cut(s) 416
NciI CCSGG 4 cut(s) 305, 341, 355, 439
NdeII GATC 1 cut(s) 128
NgoMIV GCCGGC 1 cut(s) 414
NlaIII CATG 2 cut(s) 508, 544
NlaIV GGNNCC 3 cut(s) 36, 254, 429
NmeAIII GCCGAG 1 cut(s) 254
NmuCI GTSAC 2 cut(s) 12, 116
OliI CACNNNNGTG 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PdiI GCCGGC 1 cut(s) 416
PfeI GAWTC 2 cut(s) 142, 475
PflFI GACNNNGTC 1 cut(s) 445
PkrI GCNGC 1 cut(s) 513
PleI GAGTC 2 cut(s) 131, 266
PpsI GAGTC 2 cut(s) 131, 266
PpuMI RGGWCCY 1 cut(s) 88
Psp5II RGGWCCY 1 cut(s) 88
Psp6I CCWGG 3 cut(s) 84, 318, 323
PspGI CCWGG 3 cut(s) 84, 318, 323
PspN4I GGNNCC 3 cut(s) 36, 254, 429
PspPI GGNCC 6 cut(s) 88, 252, 277, 327, 356, 428
PspPPI RGGWCCY 1 cut(s) 88
PsuI RGATCY 1 cut(s) 128
PsyI GACNNNGTC 1 cut(s) 445
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
RseI CAYNNNNRTG 1 cut(s) 114
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 1 cut(s) 512
Sau3AI GATC 1 cut(s) 128
Sau96I GGNCC 6 cut(s) 88, 252, 277, 327, 356, 428
SchI GAGTC 2 cut(s) 131, 266
ScrFI CCNGG 7 cut(s) 86, 305, 320, 325, 341, 355, 439
SduI GDGCHC 2 cut(s) 318, 395
SetI ASST 7 cut(s) 90, 103, 156, 315, 336, 501, 533
SfaNI GCATC 1 cut(s) 18
SgrAI CRCCGGYG 1 cut(s) 451
SinI GGWCC 3 cut(s) 88, 252, 428
SmiMI CAYNNNNRTG 1 cut(s) 114
SsiI CCGC 4 cut(s) 283, 292, 384, 431
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 7 cut(s) 84, 303, 318, 323, 339, 353, 437
StyI CCWWGG 2 cut(s) 171, 330
TaaI ACNGT 1 cut(s) 399
TaiI ACGT 1 cut(s) 315
TaqI TCGA 5 cut(s) 5, 63, 75, 140, 389
TfiI GAWTC 2 cut(s) 142, 475
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TseFI GTSAC 2 cut(s) 12, 116
TseI GCWGC 1 cut(s) 511
Tsp45I GTSAC 2 cut(s) 12, 116
TspGWI ACGGA 2 cut(s) 135, 468
Tth111I GACNNNGTC 1 cut(s) 445
VneI GTGCAC 1 cut(s) 314
VpaK11BI GGWCC 3 cut(s) 88, 252, 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 527
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.