FvH4_4g21970

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005813
Physical Location & Seq
Reverse (-)
24917987 .. 24920067
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21970.t2

Sequence Viewer

Length: 957 bp
ATGGGCACACACGTCTCCAAACAGGTCGAAAGGCGCAAGGCGATTTCGACTGAGAAGAAAACTCTGTGTGGTCTGCATGAAAAATGTGGCGTGGACTTTCCAGAATGTTCATACAAGCCTGCAGACAGGAAAAACTGGATGGCTGGGATCAACCCTGACAAGGTTCACATAAACCAGATTGTCTGGCCCGGAACTCATGACTCTGCAACTAACAGGATTGGAGTCCCTTGCATCACTAGGCCTTTTGCTCAGTGCCAGAACCTCTCCATCTACAAACAGCTAGTCAAAGGCACCAGAGTTCTTGACATTCGAGTTCAGGAGGATCGCCGAGTCTGTCATGGAATTCTCAAAGCTTACAACGTTGATGTTGTGATCAATGACGTCAAGAAGTTTTTATCCGAAACGGAGAAGGAGATCATCATCCTTGAAATTCGAACTGAGTTTGGACATGAAGACCCTGCTGAGTTTGACAAGTATCTGGTGGAGCAGTTTGGAGAGCTTTTGATCCACCAGGATGACAATGTGTTTCACAAGACCATTTCAGAACTGCTGCCGAAAAGGATCATATGTGTGTGGAAGCCAAGGAAGTCTCCTCAGGCCAAGGCAGGGAGTCCATTATGGAACTCCGGGCATTTGAAGGATAATTGGATTGATACGGATTTGCCATCCACAAAATTCGAGAGCAATATGAAACATTTGAGTGAGCAGCCACCGGTGACATCAAGGAAATACTTTTACAGGGTGGAGAACACTGTGACACCCCAGGCTGATAACCCTATCTTGTGTGTCAAGCCTGTGACAGGGCGGATTCATGGATATGCAAGGCTGTTTATAACTCAGTGCTTCTCTAGAGGATGTGCAGATAGGTTGCAGATCTTCTCCACAGATTTTATTGATGAGGATTTCGTTGATGCGTGCGTTGCAGTTACATATTCTAGAATTGAAGGGAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

319

Amino Acids

36.4

Weight (kDa)

8.41

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 77 - 175 8.9e-10 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AatII GACGTC 1 cut(s) 384
AccB1I GGYRCC 1 cut(s) 290
AciI CCGC 1 cut(s) 805
AclI AACGTT 1 cut(s) 360
AclWI GGATC 4 cut(s) 155, 330, 499, 569
AcsI RAATTY 3 cut(s) 342, 429, 674
AcyI GRCGYC 1 cut(s) 381
AfiI CCNNNNNNNGG 3 cut(s) 160, 606, 800
AflIII ACRYGT 1 cut(s) 10
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 3 cut(s) 428, 637, 944
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 510, 762
AluBI AGCT 3 cut(s) 280, 353, 499
AluI AGCT 3 cut(s) 280, 353, 499
Alw26I GTCTC 2 cut(s) 19, 594
AlwI GGATC 4 cut(s) 155, 330, 499, 569
AoxI GGCC 3 cut(s) 185, 239, 597
ApeKI GCWGC 2 cut(s) 550, 706
ApoI RAATTY 3 cut(s) 342, 429, 674
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
AsiGI ACCGGT 1 cut(s) 712
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 1 cut(s) 186
AsuC2I CCSGG 2 cut(s) 189, 628
AsuHPI GGTGA 1 cut(s) 727
AsuII TTCGAA 1 cut(s) 433
AxyI CCTNAGG 1 cut(s) 594
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 459
BbvI GCAGC 2 cut(s) 537, 718
BccI CCATC 3 cut(s) 133, 275, 673
BciT130I CCWGG 2 cut(s) 512, 764
BclI TGATCA 1 cut(s) 372
BcnI CCSGG 2 cut(s) 189, 628
BcoDI GTCTC 2 cut(s) 19, 594
BfaI CTAG 4 cut(s) 237, 281, 849, 936
BfmI CTRYAG 1 cut(s) 120
BglII AGATCT 1 cut(s) 873
BisI GCNGC 2 cut(s) 551, 707
BlsI GCNGC 2 cut(s) 552, 708
Bme1390I CCNGG 4 cut(s) 189, 512, 628, 764
BmgBI CACGTC 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 4 cut(s) 189, 512, 628, 764
BmsI GCATC 2 cut(s) 240, 901
BpiI GAAGAC 1 cut(s) 459
Bpu14I TTCGAA 1 cut(s) 433
BpuMI CCSGG 2 cut(s) 189, 628
BsaBI GATNNNNATC 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 581, 600, 762
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 606, 800
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 1 cut(s) 140
Bse21I CCTNAGG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 419
BseBI CCWGG 2 cut(s) 512, 764
BseDI CCNNGG 3 cut(s) 581, 600, 762
BseGI GGATG 5 cut(s) 144, 420, 520, 665, 860
BseJI GATNNNNATC 1 cut(s) 419
BseLI CCNNNNNNNGG 3 cut(s) 160, 606, 800
BseMII CTCAG 6 cut(s) 42, 263, 429, 453, 608, 851
BseNI ACTGG 1 cut(s) 140
BseRI GAGGAG 1 cut(s) 582
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 2 cut(s) 537, 718
BseYI CCCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 879
BshFI GGCC 3 cut(s) 187, 241, 599
BshNI GGYRCC 1 cut(s) 290
BshTI ACCGGT 1 cut(s) 712
BsiSI CCGG 3 cut(s) 189, 627, 713
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 3 cut(s) 160, 606, 800
BsmAI GTCTC 2 cut(s) 19, 594
BsmBI CGTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 3 cut(s) 187, 241, 599
Bsp119I TTCGAA 1 cut(s) 433
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 7 cut(s) 147, 322, 372, 414, 504, 561, 873
BspACI CCGC 1 cut(s) 805
BspANI GGCC 3 cut(s) 187, 241, 599
BspCNI CTCAG 6 cut(s) 43, 262, 430, 454, 607, 850
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 292
BspMAI CTGCAG 1 cut(s) 124
BspPI GGATC 4 cut(s) 155, 330, 499, 569
BspT104I TTCGAA 1 cut(s) 433
BspT107I GGYRCC 1 cut(s) 290
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 3 cut(s) 581, 600, 762
BssMI GATC 7 cut(s) 147, 322, 372, 414, 504, 561, 873
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 2 cut(s) 581, 600
Bst2UI CCWGG 2 cut(s) 512, 764
Bst4CI ACNGT 1 cut(s) 754
BstACI GRCGYC 1 cut(s) 381
BstBI TTCGAA 1 cut(s) 433
BstC8I GCNNGC 2 cut(s) 120, 916
BstDEI CTNAG 6 cut(s) 51, 249, 438, 462, 594, 837
BstENI CCTNNNNNAGG 1 cut(s) 798
BstF5I GGATG 5 cut(s) 144, 420, 520, 665, 860
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 7 cut(s) 150, 325, 375, 417, 507, 564, 876
BstMAI GTCTC 2 cut(s) 19, 594
BstMBI GATC 7 cut(s) 147, 322, 372, 414, 504, 561, 873
BstMWI GCNNNNNNNGC 1 cut(s) 920
BstNI CCWGG 2 cut(s) 512, 764
BstSCI CCNGG 4 cut(s) 187, 510, 626, 762
BstSFI CTRYAG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 537, 718
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 1 cut(s) 873
BstYI RGATCY 1 cut(s) 873
Bsu36I CCTNAGG 1 cut(s) 594
BsuRI GGCC 3 cut(s) 187, 241, 599
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 5 cut(s) 144, 420, 520, 665, 860
BtsIMutI CAGTG 3 cut(s) 257, 750, 845
Cac8I GCNNGC 2 cut(s) 120, 916
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 36
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 186
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 6 cut(s) 77, 197, 338, 449, 812, 954
DdeI CTNAG 6 cut(s) 51, 249, 438, 462, 594, 837
DpnI GATC 7 cut(s) 149, 324, 374, 416, 506, 563, 875
DpnII GATC 7 cut(s) 147, 322, 372, 414, 504, 561, 873
EciI GGCGGA 1 cut(s) 820
Eco130I CCWWGG 2 cut(s) 581, 600
Eco147I AGGCCT 1 cut(s) 241
Eco81I CCTNAGG 1 cut(s) 594
EcoNI CCTNNNNNAGG 1 cut(s) 798
EcoRI GAATTC 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 510, 762
EcoT14I CCWWGG 2 cut(s) 581, 600
ErhI CCWWGG 2 cut(s) 581, 600
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 6 cut(s) 80, 200, 341, 452, 815, 957
FaqI GGGAC 1 cut(s) 209
FatI CATG 6 cut(s) 76, 196, 337, 448, 811, 953
FauNDI CATATG 1 cut(s) 566
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 2 cut(s) 551, 707
FokI GGATG 5 cut(s) 151, 407, 527, 652, 867
Fsp4HI GCNGC 2 cut(s) 551, 707
FspBI CTAG 4 cut(s) 237, 281, 849, 936
GlaI GCGC 1 cut(s) 35
GluI GCNGC 2 cut(s) 551, 707
GsaI CCCAGC 1 cut(s) 147
HaeIII GGCC 3 cut(s) 187, 241, 599
HapII CCGG 3 cut(s) 189, 627, 713
HhaI GCGC 1 cut(s) 36
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 6 cut(s) 80, 200, 341, 452, 815, 957
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 5 cut(s) 200, 222, 330, 610, 808
HpaII CCGG 3 cut(s) 189, 627, 713
HphI GGTGA 1 cut(s) 727
Hpy166II GTNNAC 2 cut(s) 94, 166
Hpy188I TCNGA 2 cut(s) 400, 544
Hpy188III TCNNGA 8 cut(s) 101, 197, 302, 317, 385, 679, 849, 936
Hpy8I GTNNAC 2 cut(s) 94, 166
HpyAV CCTTC 4 cut(s) 403, 631, 938, 943
HpyCH4III ACNGT 1 cut(s) 754
HpyCH4IV ACGT 3 cut(s) 12, 360, 381
HpyCH4V TGCA 8 cut(s) 76, 122, 206, 231, 821, 860, 871, 923
HpyF10VI GCNNNNNNNGC 1 cut(s) 920
HpyF3I CTNAG 6 cut(s) 51, 249, 438, 462, 594, 837
HpySE526I ACGT 3 cut(s) 12, 360, 381
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 6 cut(s) 80, 200, 341, 452, 815, 957
HspAI GCGC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 372
Kzo9I GATC 7 cut(s) 147, 322, 372, 414, 504, 561, 873
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 2 cut(s) 537, 718
LweI GCATC 2 cut(s) 240, 901
MaeI CTAG 4 cut(s) 237, 281, 849, 936
MaeII ACGT 3 cut(s) 12, 360, 381
MaeIII GTNAC 4 cut(s) 715, 754, 796, 925
MalI GATC 7 cut(s) 149, 324, 374, 416, 506, 563, 875
MboI GATC 7 cut(s) 147, 322, 372, 414, 504, 561, 873
MboII GAAGA 3 cut(s) 67, 464, 868
MflI RGATCY 1 cut(s) 873
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 5 cut(s) 342, 429, 643, 674, 939
MlyI GAGTC 4 cut(s) 194, 231, 339, 619
MnlI CCTC 5 cut(s) 272, 313, 603, 845, 892
MslI CAYNNNNRTG 4 cut(s) 513, 569, 699, 816
MspI CCGG 3 cut(s) 189, 627, 713
MspR9I CCNGG 4 cut(s) 189, 512, 628, 764
MvaI CCWGG 2 cut(s) 512, 764
MwoI GCNNNNNNNGC 1 cut(s) 920
NciI CCSGG 2 cut(s) 189, 628
NdeI CATATG 1 cut(s) 566
NdeII GATC 7 cut(s) 147, 322, 372, 414, 504, 561, 873
NlaIII CATG 6 cut(s) 80, 200, 341, 452, 815, 957
NlaIV GGNNCC 1 cut(s) 292
NmeAIII GCCGAG 1 cut(s) 353
NmuCI GTSAC 3 cut(s) 715, 754, 796
NspV TTCGAA 1 cut(s) 433
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 241
PfeI GAWTC 1 cut(s) 808
PinAI ACCGGT 1 cut(s) 712
PkrI GCNGC 2 cut(s) 552, 708
PleI GAGTC 4 cut(s) 194, 230, 338, 618
PpsI GAGTC 4 cut(s) 194, 230, 338, 618
PsiI TTATAA 1 cut(s) 833
Psp1406I AACGTT 1 cut(s) 360
Psp6I CCWGG 2 cut(s) 510, 762
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 510, 762
PspN4I GGNNCC 1 cut(s) 292
PspPI GGNCC 1 cut(s) 186
PstI CTGCAG 1 cut(s) 124
PsuI RGATCY 1 cut(s) 873
RseI CAYNNNNRTG 4 cut(s) 513, 569, 699, 816
SatI GCNGC 2 cut(s) 551, 707
Sau3AI GATC 7 cut(s) 147, 322, 372, 414, 504, 561, 873
Sau96I GGNCC 1 cut(s) 186
SchI GAGTC 4 cut(s) 194, 231, 339, 619
ScrFI CCNGG 4 cut(s) 189, 512, 628, 764
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 2 cut(s) 240, 901
SfcI CTRYAG 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 433
SgrAI CRCCGGYG 1 cut(s) 712
SmiMI CAYNNNNRTG 4 cut(s) 513, 569, 699, 816
Sse9I AATT 5 cut(s) 342, 429, 643, 674, 939
SseBI AGGCCT 1 cut(s) 241
SsiI CCGC 1 cut(s) 805
SspMI CTAG 4 cut(s) 237, 281, 849, 936
StuI AGGCCT 1 cut(s) 241
StyD4I CCNGG 4 cut(s) 187, 510, 626, 762
StyI CCWWGG 2 cut(s) 581, 600
TaaI ACNGT 1 cut(s) 754
TaiI ACGT 3 cut(s) 15, 363, 384
TaqI TCGA 5 cut(s) 27, 47, 310, 433, 678
TasI AATT 5 cut(s) 342, 429, 643, 674, 939
TfiI GAWTC 1 cut(s) 808
TscAI CASTG 3 cut(s) 257, 757, 845
TseFI GTSAC 3 cut(s) 715, 754, 796
TseI GCWGC 2 cut(s) 550, 706
Tsp45I GTSAC 3 cut(s) 715, 754, 796
TspDTI ATGAA 5 cut(s) 93, 99, 465, 704, 800
TspGWI ACGGA 2 cut(s) 419, 671
TspRI CASTG 3 cut(s) 257, 757, 845
XagI CCTNNNNNAGG 1 cut(s) 798
XapI RAATTY 3 cut(s) 342, 429, 674
XbaI TCTAGA 2 cut(s) 848, 935
XspI CTAG 4 cut(s) 237, 281, 849, 936
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.