Rw6G001980

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005813
Physical Location & Seq
Reverse (-)
3210278 .. 3211764
1487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001980.1

Sequence Viewer

Length: 957 bp
ATGGGTACCCAGTTCTCTAAACAGGTCCAAAGGCGCAAGGCGATTTCGACTGAGAAAAAAGCTCTCTGTGATCTCTATAAAACATGTGGCGAGGACTTTCCAGAGTGTTCATACAAGCCTGCAGACAGAAAAAACTGGATGGCTGGGATCAACCCTGAGAAAGTTCACATCAACAAGATTGTCTGGCCCGGAACTCATGACTCTGCAACCAACAAGATTGGAGTCCCCTGCATCACTAGGCCTTTTGCTCAATGCCAAAAGCTTTCCATCTACAACCAGCTAGTCAAAGGCACCAGACTTCTTGACATTCGTGTTCAGGAGGATCGCCGAGTCTGTCATGGAGTTCTCACCACTTACAGCGTTGATGTTGTGATCAATGACGTCAAGAAGTTCTTATCTGAAACTGAGAAGGAGATCATCATCCTTGAAATCCGAACTGAGTTTGGACATGAAGACCCTCCTGACTTTGACAAGTATTTGGTGGAACAGTTTGGAGAGCTTTTGATCCACCAGGATGACAATGTATTCAACAAAACTATTTCGGAGTTGCTGCCGAAGAGGATCATATGCGTGTGGAAACCTAGGAAGTCTCCTCAGGCCAAGGCAGGGAGTCCATTATGGAACTCCGGGCACTTGAAGGATAATTGGATCGACACAGATTTGCCATCCACGAAATTCGAAAGCAATCTGAAGAATTTGAGTGAGCAGCCACCGGTTACATCACGGAAGTACTTTTACAGGGTGGAGAACACTGTGACACCCCAGGCTGATAACCCTATCTTGTGTGTGAAGCCTGTGACAAGGCGGATTCATGGATATGCAAGGCTGTTTATAACTCAGTGCTTCTCTAGAGGTTGTGCAGATAGGTTGCAGGTCTTCTCCACTGATTTTATTGATGAGGATTTCGTTGATGCGTGCGCCGCGGTTACATATTCGAGAATTGAAGGGAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

318

Amino Acids

36.49

Weight (kDa)

8.54

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 63 - 177 6.7e-10 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AatII GACGTC 1 cut(s) 384
Acc36I ACCTGC 1 cut(s) 862
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 2 cut(s) 5, 290
AccII CGCG 1 cut(s) 923
AciI CCGC 3 cut(s) 805, 921, 923
AclWI GGATC 5 cut(s) 155, 330, 499, 569, 656
AcsI RAATTY 2 cut(s) 674, 694
AcuI CTGAAG 1 cut(s) 710
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 2 cut(s) 7, 731
AfiI CCNNNNNNNGG 1 cut(s) 606
AflIII ACRYGT 1 cut(s) 83
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 4 cut(s) 428, 529, 637, 944
AjnI CCWGG 2 cut(s) 510, 762
AluBI AGCT 4 cut(s) 62, 262, 280, 499
AluI AGCT 4 cut(s) 62, 262, 280, 499
Alw26I GTCTC 1 cut(s) 594
AlwI GGATC 5 cut(s) 155, 330, 499, 569, 656
AoxI GGCC 3 cut(s) 185, 239, 597
ApeKI GCWGC 2 cut(s) 550, 706
ApoI RAATTY 2 cut(s) 674, 694
AsiGI ACCGGT 1 cut(s) 712
Asp718I GGTACC 1 cut(s) 5
AspA2I CCTAGG 1 cut(s) 581
AspLEI GCGC 2 cut(s) 36, 920
AspS9I GGNCC 2 cut(s) 25, 186
AsuC2I CCSGG 2 cut(s) 189, 628
AsuHPI GGTGA 1 cut(s) 340
AsuII TTCGAA 1 cut(s) 678
AvaII GGWCC 1 cut(s) 25
AvrII CCTAGG 1 cut(s) 581
AxyI CCTNAGG 1 cut(s) 594
BaeGI GKGCMC 1 cut(s) 633
BanI GGYRCC 2 cut(s) 5, 290
BarI GAAGNNNNNNTAC 2 cut(s) 719, 751
BbsI GAAGAC 2 cut(s) 459, 868
BbvI GCAGC 2 cut(s) 537, 718
BccI CCATC 3 cut(s) 133, 275, 673
BciT130I CCWGG 2 cut(s) 512, 764
BclI TGATCA 1 cut(s) 372
BcnI CCSGG 2 cut(s) 189, 628
BcoDI GTCTC 1 cut(s) 594
BfaI CTAG 4 cut(s) 237, 281, 582, 849
BfmI CTRYAG 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 862
BisI GCNGC 3 cut(s) 551, 707, 921
BlnI CCTAGG 1 cut(s) 581
BlsI GCNGC 3 cut(s) 552, 708, 922
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 4 cut(s) 189, 512, 628, 764
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 2 cut(s) 25, 186
BmiI GGNNCC 2 cut(s) 7, 292
BmrFI CCNGG 4 cut(s) 189, 512, 628, 764
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 2 cut(s) 240, 901
BmuI ACTGGG 1 cut(s) 4
BpiI GAAGAC 2 cut(s) 459, 868
Bpu14I TTCGAA 1 cut(s) 678
BpuMI CCSGG 2 cut(s) 189, 628
BsaBI GATNNNNATC 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 4 cut(s) 581, 600, 762, 921
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 2 cut(s) 10, 140
Bse21I CCTNAGG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 419
BseBI CCWGG 2 cut(s) 512, 764
BseDI CCNNGG 4 cut(s) 581, 600, 762, 921
BseGI GGATG 4 cut(s) 144, 420, 520, 665
BseJI GATNNNNATC 1 cut(s) 419
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMII CTCAG 6 cut(s) 42, 147, 396, 429, 608, 851
BseNI ACTGG 2 cut(s) 10, 140
BseRI GAGGAG 1 cut(s) 582
BseSI GKGCMC 1 cut(s) 633
BseXI GCAGC 2 cut(s) 537, 718
BseYI CCCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 879
Bsh1236I CGCG 1 cut(s) 923
BshFI GGCC 3 cut(s) 187, 241, 599
BshNI GGYRCC 2 cut(s) 5, 290
BshTI ACCGGT 1 cut(s) 712
BsiSI CCGG 3 cut(s) 189, 627, 713
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 1 cut(s) 594
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 3 cut(s) 187, 241, 599
Bsp119I TTCGAA 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 633
Bsp143I GATC 8 cut(s) 70, 147, 322, 372, 414, 504, 561, 648
BspACI CCGC 3 cut(s) 805, 921, 923
BspANI GGCC 3 cut(s) 187, 241, 599
BspCNI CTCAG 6 cut(s) 43, 148, 397, 430, 607, 850
BspFNI CGCG 1 cut(s) 923
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 7, 292
BspMAI CTGCAG 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 862
BspPI GGATC 5 cut(s) 155, 330, 499, 569, 656
BspT104I TTCGAA 1 cut(s) 678
BspT107I GGYRCC 2 cut(s) 5, 290
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 2 cut(s) 10, 140
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 4 cut(s) 581, 600, 762, 921
BssMI GATC 8 cut(s) 70, 147, 322, 372, 414, 504, 561, 648
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 2 cut(s) 581, 600
Bst2UI CCWGG 2 cut(s) 512, 764
Bst4CI ACNGT 2 cut(s) 489, 754
Bst6I CTCTTC 1 cut(s) 551
BstACI GRCGYC 1 cut(s) 381
BstBI TTCGAA 1 cut(s) 678
BstC8I GCNNGC 2 cut(s) 120, 916
BstDEI CTNAG 6 cut(s) 51, 156, 405, 438, 594, 837
BstDSI CCRYGG 1 cut(s) 921
BstF5I GGATG 4 cut(s) 144, 420, 520, 665
BstFNI CGCG 1 cut(s) 923
BstHHI GCGC 2 cut(s) 36, 920
BstKTI GATC 8 cut(s) 73, 150, 325, 375, 417, 507, 564, 651
BstMAI GTCTC 1 cut(s) 594
BstMBI GATC 8 cut(s) 70, 147, 322, 372, 414, 504, 561, 648
BstMWI GCNNNNNNNGC 1 cut(s) 920
BstNI CCWGG 2 cut(s) 512, 764
BstNSI RCATGY 1 cut(s) 87
BstSCI CCNGG 4 cut(s) 187, 510, 626, 762
BstSFI CTRYAG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 633
BstUI CGCG 1 cut(s) 923
BstV1I GCAGC 2 cut(s) 537, 718
BstV2I GAAGAC 2 cut(s) 459, 868
Bsu36I CCTNAGG 1 cut(s) 594
BsuRI GGCC 3 cut(s) 187, 241, 599
BtgI CCRYGG 1 cut(s) 921
BtsCI GGATG 4 cut(s) 144, 420, 520, 665
BtsIMutI CAGTG 3 cut(s) 750, 845, 882
BveI ACCTGC 1 cut(s) 862
Cac8I GCNNGC 2 cut(s) 120, 916
CciI TCATGA 1 cut(s) 196
CfoI GCGC 2 cut(s) 36, 920
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 2 cut(s) 25, 186
Cfr42I CCGCGG 1 cut(s) 924
Csp6I GTAC 2 cut(s) 6, 730
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 6 cut(s) 84, 197, 338, 449, 812, 954
CviQI GTAC 2 cut(s) 6, 730
DdeI CTNAG 6 cut(s) 51, 156, 405, 438, 594, 837
DpnI GATC 8 cut(s) 72, 149, 324, 374, 416, 506, 563, 650
DpnII GATC 8 cut(s) 70, 147, 322, 372, 414, 504, 561, 648
Eam1104I CTCTTC 1 cut(s) 551
EarI CTCTTC 1 cut(s) 551
EciI GGCGGA 1 cut(s) 820
Eco130I CCWWGG 2 cut(s) 581, 600
Eco147I AGGCCT 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 710
Eco81I CCTNAGG 1 cut(s) 594
EcoRII CCWGG 2 cut(s) 510, 762
EcoT14I CCWWGG 2 cut(s) 581, 600
ErhI CCWWGG 2 cut(s) 581, 600
FaeI CATG 6 cut(s) 87, 200, 341, 452, 815, 957
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 1 cut(s) 209
FatI CATG 6 cut(s) 83, 196, 337, 448, 811, 953
FauNDI CATATG 1 cut(s) 566
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 551, 707, 921
FokI GGATG 4 cut(s) 151, 407, 527, 652
Fsp4HI GCNGC 3 cut(s) 551, 707, 921
FspBI CTAG 4 cut(s) 237, 281, 582, 849
GlaI GCGC 2 cut(s) 35, 919
GluI GCNGC 3 cut(s) 551, 707, 921
GsaI CCCAGC 1 cut(s) 147
HaeIII GGCC 3 cut(s) 187, 241, 599
HapII CCGG 3 cut(s) 189, 627, 713
HhaI GCGC 2 cut(s) 36, 920
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 6 cut(s) 87, 200, 341, 452, 815, 957
Hin6I GCGC 2 cut(s) 34, 918
HinP1I GCGC 2 cut(s) 34, 918
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 5 cut(s) 200, 222, 330, 610, 808
HpaII CCGG 3 cut(s) 189, 627, 713
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 4 cut(s) 400, 434, 544, 690
Hpy188III TCNNGA 8 cut(s) 101, 197, 302, 317, 385, 461, 849, 936
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 3 cut(s) 403, 631, 938
HpyCH4III ACNGT 2 cut(s) 489, 754
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 6 cut(s) 122, 206, 231, 821, 860, 871
HpyF10VI GCNNNNNNNGC 1 cut(s) 920
HpyF3I CTNAG 6 cut(s) 51, 156, 405, 438, 594, 837
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 6 cut(s) 87, 200, 341, 452, 815, 957
HspAI GCGC 2 cut(s) 34, 918
KpnI GGTACC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 372
KspI CCGCGG 1 cut(s) 924
Kzo9I GATC 8 cut(s) 70, 147, 322, 372, 414, 504, 561, 648
Lsp1109I GCAGC 2 cut(s) 537, 718
LweI GCATC 2 cut(s) 240, 901
MaeI CTAG 4 cut(s) 237, 281, 582, 849
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 4 cut(s) 715, 754, 796, 925
MalI GATC 8 cut(s) 72, 149, 324, 374, 416, 506, 563, 650
MboI GATC 8 cut(s) 70, 147, 322, 372, 414, 504, 561, 648
MboII GAAGA 4 cut(s) 464, 568, 703, 868
MhlI GDGCHC 1 cut(s) 633
MluCI AATT 4 cut(s) 643, 674, 694, 939
MlyI GAGTC 4 cut(s) 194, 231, 339, 619
MnlI CCTC 7 cut(s) 85, 313, 468, 552, 603, 845, 892
MslI CAYNNNNRTG 3 cut(s) 513, 569, 816
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 3 cut(s) 189, 627, 713
MspR9I CCNGG 4 cut(s) 189, 512, 628, 764
MvaI CCWGG 2 cut(s) 512, 764
MvnI CGCG 1 cut(s) 923
MwoI GCNNNNNNNGC 1 cut(s) 920
NciI CCSGG 2 cut(s) 189, 628
NdeI CATATG 1 cut(s) 566
NdeII GATC 8 cut(s) 70, 147, 322, 372, 414, 504, 561, 648
NlaIII CATG 6 cut(s) 87, 200, 341, 452, 815, 957
NlaIV GGNNCC 2 cut(s) 7, 292
NmeAIII GCCGAG 1 cut(s) 353
NmuCI GTSAC 2 cut(s) 754, 796
NspI RCATGY 1 cut(s) 87
NspV TTCGAA 1 cut(s) 678
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 241
PciI ACATGT 1 cut(s) 83
PfeI GAWTC 1 cut(s) 808
PinAI ACCGGT 1 cut(s) 712
PkrI GCNGC 3 cut(s) 552, 708, 922
PleI GAGTC 4 cut(s) 194, 230, 338, 618
PpsI GAGTC 4 cut(s) 194, 230, 338, 618
PscI ACATGT 1 cut(s) 83
PsiI TTATAA 1 cut(s) 833
Psp6I CCWGG 2 cut(s) 510, 762
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 510, 762
PspN4I GGNNCC 2 cut(s) 7, 292
PspPI GGNCC 2 cut(s) 25, 186
PstI CTGCAG 1 cut(s) 124
RsaI GTAC 2 cut(s) 7, 731
RsaNI GTAC 2 cut(s) 6, 730
RseI CAYNNNNRTG 3 cut(s) 513, 569, 816
SacII CCGCGG 1 cut(s) 924
SatI GCNGC 3 cut(s) 551, 707, 921
Sau3AI GATC 8 cut(s) 70, 147, 322, 372, 414, 504, 561, 648
Sau96I GGNCC 2 cut(s) 25, 186
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 4 cut(s) 194, 231, 339, 619
ScrFI CCNGG 4 cut(s) 189, 512, 628, 764
SduI GDGCHC 1 cut(s) 633
SfaNI GCATC 2 cut(s) 240, 901
SfcI CTRYAG 1 cut(s) 120
Sfr303I CCGCGG 1 cut(s) 924
SfuI TTCGAA 1 cut(s) 678
SgrBI CCGCGG 1 cut(s) 924
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 3 cut(s) 513, 569, 816
Sse9I AATT 4 cut(s) 643, 674, 694, 939
SseBI AGGCCT 1 cut(s) 241
SsiI CCGC 3 cut(s) 805, 921, 923
SspMI CTAG 4 cut(s) 237, 281, 582, 849
StuI AGGCCT 1 cut(s) 241
StyD4I CCNGG 4 cut(s) 187, 510, 626, 762
StyI CCWWGG 2 cut(s) 581, 600
TaaI ACNGT 2 cut(s) 489, 754
TaiI ACGT 1 cut(s) 384
TaqI TCGA 4 cut(s) 47, 651, 678, 935
TasI AATT 4 cut(s) 643, 674, 694, 939
TatI WGTACW 1 cut(s) 729
TauI GCSGC 1 cut(s) 923
TfiI GAWTC 1 cut(s) 808
TscAI CASTG 3 cut(s) 757, 845, 889
TseFI GTSAC 2 cut(s) 754, 796
TseI GCWGC 2 cut(s) 550, 706
Tsp45I GTSAC 2 cut(s) 754, 796
TspDTI ATGAA 3 cut(s) 99, 465, 800
TspGWI ACGGA 1 cut(s) 739
TspRI CASTG 3 cut(s) 757, 845, 889
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 2 cut(s) 674, 694
XbaI TCTAGA 1 cut(s) 848
XceI RCATGY 1 cut(s) 87
XmaJI CCTAGG 1 cut(s) 581
XspI CTAG 4 cut(s) 237, 281, 582, 849
ZraI GACGTC 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.