MD05G1118000.v1.1

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005813
Physical Location & Seq
Forward (+)
23893128 .. 23894084
957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1118000.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGGTTCCCATGTCTCGAAGCAGGTAGAACGTCGGAAGGCGATCGCGGTGGAAAAACACACACTGTGTGATCTTCACCAGAGCTGTGGTGAAGAGTTTCCATCATGTGCCTACAAGCCTTCGGACAGAAAAAGCTGGATGGCTGGGATCAACCCTGAGAAACTTCACATAAATAAGATTGTATGGCCTGGAACTCATGACTCTGCAACCAACAAGATTGGATTTCCGTTCATCACTAGGCCTTTTGCTCAGTGTCAAAATCTGTCCATCTACCAGCAGCTCGAGAAGGGCGCCAGAGTTCTCGATATTCGAATCCAGAAGGACCGAAAAGTCTGCCACGGAGTCCTCCTCACTTACAACGTTGATGCTGTGATCAGCGACGTCAAGAAGTTTTTGTCCGAAACGCAGTATGAGATCATAATACTCGAAATCCGAACTGAATTCGGCCACGAAGATCCTCCTGAATTTGATAAGTACTTGGTTGATCAGCTCGGCGAGTTTTTAATCCCCCAAGATGATCATGTTTTCGACAAGACTATTTCGGAACTGCTGCCGAAGAGAGTGATATGCGTGTGGAAGCCGAGGAAGTCACCTCAGCCGAAGGCAGGGAGTCCTCTGTGGAATGCAGGGCATTTGAAGGACAACTGGATCGACACGGACTTGCCATCCACGAAGTTCGAGAGCAACCTGAAACATTTGAGTAATCAGCCGCCGGTTTCATCAAGGAAGTTCTTTTACAGGGTGGAGAACACGGTCACGCCCAAGGCTGATAACCCGGTTTTGTGTGTCAAGCCTGTGACGGGACGGATTCATGGATACGCGAGGCTGTTTATAACTCAGTGCTTCTCTAGAGGTATTGCGGATAGGTTGCAGATCCTTTCTACAGATTTTATAGACGAGGATTTCGTGGACGCTTGCGTTGCGGTTACATATGCAAGAGTTGAAGGGAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

319

Amino Acids

36.26

Weight (kDa)

8.29

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 65 - 174 1.3e-09 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AasI GACNNNNNNGTC 1 cut(s) 329
AatII GACGTC 1 cut(s) 384
Acc36I ACCTGC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 290
AccII CGCG 2 cut(s) 47, 821
AciI CCGC 4 cut(s) 47, 710, 860, 923
AclI AACGTT 1 cut(s) 360
AclWI GGATC 4 cut(s) 155, 449, 656, 868
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 2 cut(s) 440, 464
AcyI GRCGYC 2 cut(s) 291, 381
AdeI CACNNNGTG 2 cut(s) 66, 68
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 2 cut(s) 605, 800
AgsI TTSAA 2 cut(s) 637, 944
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 4 cut(s) 84, 135, 280, 490
AluI AGCT 4 cut(s) 84, 135, 280, 490
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 4 cut(s) 155, 449, 656, 868
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 3 cut(s) 185, 239, 445
ApeKI GCWGC 2 cut(s) 277, 550
ApoI RAATTY 2 cut(s) 440, 464
ArsI GACNNNNNNTTYG 2 cut(s) 887, 919
AsiSI GCGATCGC 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 3 cut(s) 68, 101, 582
AsuII TTCGAA 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 322
BanI GGYRCC 1 cut(s) 290
BarI GAAGNNNNNNTAC 2 cut(s) 719, 751
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 2 cut(s) 289, 537
BccI CCATC 4 cut(s) 109, 133, 275, 673
BcgI CGANNNNNNTGC 2 cut(s) 315, 349
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 1 cut(s) 809
BclI TGATCA 3 cut(s) 372, 484, 517
BcnI CCSGG 1 cut(s) 776
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 2 cut(s) 237, 849
BfmI CTRYAG 1 cut(s) 882
BfoI RGCGCY 1 cut(s) 294
BfuAI ACCTGC 1 cut(s) 13
BfuI GTATCC 1 cut(s) 809
BisI GCNGC 3 cut(s) 278, 551, 710
BlsI GCNGC 3 cut(s) 279, 552, 711
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 2 cut(s) 189, 776
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 7, 292
BmrFI CCNGG 2 cut(s) 189, 776
BmsI GCATC 1 cut(s) 355
BplI GAGNNNNNCTC 2 cut(s) 333, 365
Bpu10I CCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 310
BpuMI CCSGG 1 cut(s) 776
BsaHI GRCGYC 2 cut(s) 291, 381
BsaJI CCNNGG 3 cut(s) 337, 581, 762
Bsc4I CCNNNNNNNGG 2 cut(s) 605, 800
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 1 cut(s) 650
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 3 cut(s) 337, 581, 762
BseGI GGATG 2 cut(s) 144, 665
BseLI CCNNNNNNNGG 2 cut(s) 605, 800
BseMII CTCAG 4 cut(s) 147, 263, 608, 851
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 2 cut(s) 289, 537
BseYI CCCAGC 1 cut(s) 143
Bsh1236I CGCG 2 cut(s) 47, 821
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 3 cut(s) 187, 241, 447
BshNI GGYRCC 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 713, 776
BslFI GGGAC 1 cut(s) 816
BslI CCNNNNNNNGG 2 cut(s) 605, 800
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 816
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 3 cut(s) 187, 241, 447
BsoBI CYCGRG 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 310
BspACI CCGC 4 cut(s) 47, 710, 860, 923
BspANI GGCC 3 cut(s) 187, 241, 447
BspCNI CTCAG 4 cut(s) 148, 262, 607, 850
BspFNI CGCG 2 cut(s) 47, 821
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 7, 292
BspMI ACCTGC 1 cut(s) 13
BspPI GGATC 4 cut(s) 155, 449, 656, 868
BspT104I TTCGAA 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 290
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 1 cut(s) 650
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 3 cut(s) 337, 581, 762
BssNI GRCGYC 2 cut(s) 291, 381
BssT1I CCWWGG 1 cut(s) 762
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 66, 754
Bst6I CTCTTC 2 cut(s) 87, 551
BstACI GRCGYC 2 cut(s) 291, 381
BstBI TTCGAA 1 cut(s) 310
BstC8I GCNNGC 1 cut(s) 916
BstDEI CTNAG 4 cut(s) 156, 249, 594, 837
BstDSI CCRYGG 1 cut(s) 337
BstF5I GGATG 2 cut(s) 144, 665
BstFNI CGCG 2 cut(s) 47, 821
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 1 cut(s) 293
BstMAI GTCTC 1 cut(s) 19
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 1 cut(s) 920
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 2 cut(s) 187, 774
BstSFI CTRYAG 1 cut(s) 882
BstUI CGCG 2 cut(s) 47, 821
BstV1I GCAGC 2 cut(s) 289, 537
BstX2I RGATCY 2 cut(s) 454, 873
BstXI CCANNNNNNTGG 1 cut(s) 86
BstYI RGATCY 2 cut(s) 454, 873
BsuI GTATCC 1 cut(s) 809
BsuRI GGCC 3 cut(s) 187, 241, 447
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 2 cut(s) 144, 665
BtsIMutI CAGTG 3 cut(s) 62, 257, 845
BveI ACCTGC 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 916
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 293
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 322
CseI GACGC 1 cut(s) 920
Csp6I GTAC 1 cut(s) 475
CviAII CATG 6 cut(s) 11, 105, 197, 521, 812, 954
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 4 cut(s) 156, 249, 594, 837
DinI GGCGCC 1 cut(s) 292
DraIII CACNNNGTG 2 cut(s) 66, 68
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 87, 551
EarI CTCTTC 2 cut(s) 87, 551
Eco130I CCWWGG 1 cut(s) 762
Eco147I AGGCCT 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 762
EgeI GGCGCC 1 cut(s) 292
EheI GGCGCC 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 6 cut(s) 14, 108, 200, 524, 815, 957
FaqI GGGAC 1 cut(s) 816
FatI CATG 6 cut(s) 10, 104, 196, 520, 811, 953
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 3 cut(s) 372, 484, 517
Fnu4HI GCNGC 3 cut(s) 278, 551, 710
FokI GGATG 2 cut(s) 151, 652
Fsp4HI GCNGC 3 cut(s) 278, 551, 710
FspBI CTAG 2 cut(s) 237, 849
GlaI GCGC 1 cut(s) 292
GluI GCNGC 3 cut(s) 278, 551, 710
GsaI CCCAGC 1 cut(s) 147
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 3 cut(s) 187, 241, 447
HapII CCGG 2 cut(s) 713, 776
HgaI GACGC 1 cut(s) 920
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 2 cut(s) 291, 381
Hin1II CATG 6 cut(s) 14, 108, 200, 524, 815, 957
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HinfI GANTC 5 cut(s) 200, 312, 342, 610, 808
HpaII CCGG 2 cut(s) 713, 776
HphI GGTGA 3 cut(s) 68, 101, 582
Hpy166II GTNNAC 1 cut(s) 910
Hpy188I TCNGA 5 cut(s) 36, 124, 400, 434, 544
Hpy188III TCNNGA 9 cut(s) 16, 197, 283, 302, 316, 385, 461, 679, 849
Hpy8I GTNNAC 1 cut(s) 910
Hpy99I CGWCG 2 cut(s) 36, 383
HpyAV CCTTC 7 cut(s) 31, 129, 280, 313, 595, 631, 938
HpyCH4III ACNGT 2 cut(s) 66, 754
HpyCH4IV ACGT 3 cut(s) 31, 360, 381
HpyCH4V TGCA 4 cut(s) 206, 626, 871, 935
HpyF10VI GCNNNNNNNGC 1 cut(s) 920
HpyF3I CTNAG 4 cut(s) 156, 249, 594, 837
HpySE526I ACGT 3 cut(s) 31, 360, 381
Hsp92I GRCGYC 2 cut(s) 291, 381
Hsp92II CATG 6 cut(s) 14, 108, 200, 524, 815, 957
HspAI GCGC 1 cut(s) 291
KasI GGCGCC 1 cut(s) 290
Ksp22I TGATCA 3 cut(s) 372, 484, 517
Lsp1109I GCAGC 2 cut(s) 289, 537
LweI GCATC 1 cut(s) 355
MaeI CTAG 2 cut(s) 237, 849
MaeII ACGT 3 cut(s) 31, 360, 381
MaeIII GTNAC 4 cut(s) 588, 754, 796, 925
MboII GAAGA 4 cut(s) 65, 104, 464, 568
MflI RGATCY 2 cut(s) 454, 873
MluCI AATT 2 cut(s) 440, 464
Mly113I GGCGCC 1 cut(s) 291
MlyI GAGTC 3 cut(s) 194, 351, 619
MmeI TCCRAC 1 cut(s) 14
MnlI CCTC 9 cut(s) 356, 359, 468, 576, 603, 624, 816, 845, 892
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 1 cut(s) 503
MspI CCGG 2 cut(s) 713, 776
MspR9I CCNGG 2 cut(s) 189, 776
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 2 cut(s) 47, 821
MwoI GCNNNNNNNGC 1 cut(s) 920
NarI GGCGCC 1 cut(s) 291
NciI CCSGG 1 cut(s) 776
NdeI CATATG 1 cut(s) 931
NlaIII CATG 6 cut(s) 14, 108, 200, 524, 815, 957
NlaIV GGNNCC 2 cut(s) 7, 292
NmeAIII GCCGAG 2 cut(s) 471, 606
NmuCI GTSAC 3 cut(s) 588, 754, 796
NspV TTCGAA 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 281
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PctI GAATGC 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 2 cut(s) 312, 808
PkrI GCNGC 3 cut(s) 279, 552, 711
Ple19I CGATCG 1 cut(s) 45
PleI GAGTC 3 cut(s) 194, 350, 618
PluTI GGCGCC 1 cut(s) 294
PpsI GAGTC 3 cut(s) 194, 350, 618
PsiI TTATAA 1 cut(s) 833
Psp1406I AACGTT 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 7, 292
PspPI GGNCC 1 cut(s) 322
PsuI RGATCY 2 cut(s) 454, 873
PvuI CGATCG 1 cut(s) 45
RgaI GCGATCGC 1 cut(s) 45
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 3 cut(s) 278, 551, 710
Sau96I GGNCC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 3 cut(s) 194, 351, 619
ScrFI CCNGG 2 cut(s) 189, 776
SfaAI GCGATCGC 1 cut(s) 45
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 882
SfoI GGCGCC 1 cut(s) 292
Sfr274I CTCGAG 1 cut(s) 281
SfuI TTCGAA 1 cut(s) 310
SgfI GCGATCGC 1 cut(s) 45
SinI GGWCC 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 2 cut(s) 440, 464
SseBI AGGCCT 1 cut(s) 241
SsiI CCGC 4 cut(s) 47, 710, 860, 923
SspDI GGCGCC 1 cut(s) 290
SspMI CTAG 2 cut(s) 237, 849
StuI AGGCCT 1 cut(s) 241
StyD4I CCNGG 2 cut(s) 187, 774
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 2 cut(s) 66, 754
TaiI ACGT 3 cut(s) 34, 363, 384
TaqI TCGA 8 cut(s) 17, 282, 303, 310, 426, 528, 651, 678
TaqII GACCGA 1 cut(s) 339
TasI AATT 2 cut(s) 440, 464
TatI WGTACW 1 cut(s) 474
TauI GCSGC 1 cut(s) 712
TfiI GAWTC 2 cut(s) 312, 808
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 3 cut(s) 69, 257, 845
TseFI GTSAC 3 cut(s) 588, 754, 796
TseI GCWGC 2 cut(s) 277, 550
Tsp45I GTSAC 3 cut(s) 588, 754, 796
TspDTI ATGAA 3 cut(s) 220, 708, 800
TspGWI ACGGA 4 cut(s) 216, 354, 671, 820
TspRI CASTG 3 cut(s) 69, 257, 845
VpaK11BI GGWCC 1 cut(s) 322
XapI RAATTY 2 cut(s) 440, 464
XbaI TCTAGA 1 cut(s) 848
XhoI CTCGAG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 2 cut(s) 237, 849
ZraI GACGTC 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.