pycom05g10990

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005813
Physical Location & Seq
Forward (+)
14469710 .. 14470669
960 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g10990.1

Sequence Viewer

Length: 960 bp
ATGGGTTCCTGCTTCCCTAAACAGCTCGAACGCCGGAAGGCGATCTCAACGGAGCGGAAAGCACTACGCGATCTCCACCAAAGCGTTGGTGAGGACTTCCCAGCATGTGCCTACAGGCCTCCAGACAGGAAAAATTGGATGGCGGGAATCAACCCGAAAAAAGTTCACATAAACAAAATTGTATGGCCGGGAACTCATGATTCTGCAACCGACAAGATTGGAATTCCATGCATCACTAGGCCTTTTGGACAATGCCAAACAATGTCTATATACAAACAGCTTGTCACAGGCACCAGAGTGCTTGACATTCGGGTTCAAAAAGATCGCCGGGTGTGCCACGGAATTCTTATCACGTACCATATTGATGTTGTGATCAAGGACATTAAAAAATTTCTGTCCGAAACAAAGTCTGAGATCGTACTCCTTGAAATCAGGACGGAATATGGGCACCAAGACCCTCCTGATTTCGATAAGTATTTGGTGGATCAGCTCGGAGAGGTCCTTATCAACCAGGACGACAATGTGTTCAACAAGACTATTTCAGAGTTGTTGCCGAAGCGAATAATATGCGTGTGGAAGCCGAGGAATTCACCTTCCCCTGAGGCAGGGGCGCCTCTGTGGAATTCTGTCTACTTGAATGATGATTGGATTGACACGGATTTGCCATCAACGAAATTCAAGAACAATTTGAAGTGTTTGAGTGAACAACCTCCAATTTCATCAAGGAAATTTTTTTACAGGGTGGAGAACACAGTGACACCTCAGGCTGATAACCCTATTGTGTGTGTTAGACCTGTGACCCAACGGATTCACGAGTTTGCGAGGTTGTTTATAACGGAGTGCTTCTGTAGGGGTATTGCAGATAGGTTGCAAATCTTCTCTACTGATTTTATAGATGAGGATTTTGTTGATGCATGTGTTGCTGTTACGTACTCAAGAATTGATCACGGGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

320

Amino Acids

36.66

Weight (kDa)

8.42

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 64 - 177 3.6e-10 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AccB1I GGYRCC 3 cut(s) 290, 447, 610
AccBSI CCGCTC 1 cut(s) 55
AccI GTMKAC 1 cut(s) 630
AccII CGCG 1 cut(s) 69
AciI CCGC 2 cut(s) 55, 143
AclWI GGATC 1 cut(s) 492
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 7 cut(s) 222, 342, 389, 586, 622, 674, 728
AcyI GRCGYC 1 cut(s) 611
AfaI GTAC 3 cut(s) 356, 420, 932
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 6 cut(s) 317, 428, 529, 637, 679, 691
AjnI CCWGG 1 cut(s) 510
AleI CACNNNNGTG 1 cut(s) 296
AluBI AGCT 3 cut(s) 25, 280, 490
AluI AGCT 3 cut(s) 25, 280, 490
AlwI GGATC 1 cut(s) 492
AoxI GGCC 3 cut(s) 116, 185, 239
ApoI RAATTY 7 cut(s) 222, 342, 389, 586, 622, 674, 728
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 1 cut(s) 499
AsuC2I CCSGG 2 cut(s) 189, 329
AsuHPI GGTGA 2 cut(s) 101, 582
AvaII GGWCC 1 cut(s) 499
AxyI CCTNAGG 2 cut(s) 600, 762
BaeGI GKGCMC 1 cut(s) 450
BanI GGYRCC 3 cut(s) 290, 447, 610
BauI CACGAG 1 cut(s) 812
BccI CCATC 2 cut(s) 133, 673
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 1 cut(s) 512
BclI TGATCA 2 cut(s) 372, 943
BcnI CCSGG 2 cut(s) 189, 329
BfaI CTAG 1 cut(s) 237
BfmI CTRYAG 2 cut(s) 112, 847
BfoI RGCGCY 1 cut(s) 614
Bme1390I CCNGG 3 cut(s) 189, 329, 512
Bme18I GGWCC 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 4 cut(s) 7, 292, 449, 612
BmrFI CCNGG 3 cut(s) 189, 329, 512
BmsI GCATC 2 cut(s) 240, 901
BpmI CTGGAG 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 2 cut(s) 189, 329
BsaAI YACGTR 2 cut(s) 354, 930
BsaHI GRCGYC 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 337, 581
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse21I CCTNAGG 2 cut(s) 600, 762
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 2 cut(s) 337, 581
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMII CTCAG 3 cut(s) 402, 591, 776
BseSI GKGCMC 1 cut(s) 450
BseYI CCCAGC 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 3 cut(s) 118, 187, 241
BshNI GGYRCC 3 cut(s) 290, 447, 610
BsiSI CCGG 3 cut(s) 34, 188, 328
BslI CCNNNNNNNGG 1 cut(s) 605
BsnI GGCC 3 cut(s) 118, 187, 241
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 7 cut(s) 42, 70, 322, 372, 414, 484, 943
BspACI CCGC 2 cut(s) 55, 143
BspANI GGCC 3 cut(s) 118, 187, 241
BspCNI CTCAG 3 cut(s) 403, 592, 775
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 4 cut(s) 7, 292, 449, 612
BspPI GGATC 1 cut(s) 492
BspT107I GGYRCC 3 cut(s) 290, 447, 610
BsrBI CCGCTC 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 337, 581
BssMI GATC 7 cut(s) 42, 70, 322, 372, 414, 484, 943
BssNI GRCGYC 1 cut(s) 611
BssSI CACGAG 1 cut(s) 812
Bst2BI CACGAG 1 cut(s) 812
Bst2UI CCWGG 1 cut(s) 512
Bst4CI ACNGT 1 cut(s) 754
BstACI GRCGYC 1 cut(s) 611
BstAPI GCANNNNNTGC 1 cut(s) 920
BstBAI YACGTR 2 cut(s) 354, 930
BstDEI CTNAG 3 cut(s) 411, 600, 762
BstDSI CCRYGG 1 cut(s) 337
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 1 cut(s) 144
BstFNI CGCG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 614
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 7 cut(s) 45, 73, 325, 375, 417, 487, 946
BstMBI GATC 7 cut(s) 42, 70, 322, 372, 414, 484, 943
BstMWI GCNNNNNNNGC 2 cut(s) 333, 920
BstNI CCWGG 1 cut(s) 512
BstNSI RCATGY 2 cut(s) 108, 918
BstSCI CCNGG 3 cut(s) 187, 327, 510
BstSFI CTRYAG 2 cut(s) 112, 847
BstSLI GKGCMC 1 cut(s) 450
BstSNI TACGTA 1 cut(s) 930
BstUI CGCG 1 cut(s) 69
BstXI CCANNNNNNTGG 1 cut(s) 86
Bsu36I CCTNAGG 2 cut(s) 600, 762
BsuRI GGCC 3 cut(s) 118, 187, 241
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 759
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 613
Cfr13I GGNCC 1 cut(s) 499
Csp6I GTAC 3 cut(s) 355, 419, 931
CviAII CATG 4 cut(s) 105, 197, 228, 915
CviJI RGCY 9 cut(s) 25, 118, 187, 241, 280, 490, 580, 767, 956
CviKI_1 RGCY 9 cut(s) 25, 118, 187, 241, 280, 490, 580, 767, 956
CviQI GTAC 3 cut(s) 355, 419, 931
DdeI CTNAG 3 cut(s) 411, 600, 762
DinI GGCGCC 1 cut(s) 612
DpnI GATC 7 cut(s) 44, 72, 324, 374, 416, 486, 945
DpnII GATC 7 cut(s) 42, 70, 322, 372, 414, 484, 943
EaeI YGGCCR 1 cut(s) 185
Eco105I TACGTA 1 cut(s) 930
Eco147I AGGCCT 2 cut(s) 118, 241
Eco47I GGWCC 1 cut(s) 499
Eco81I CCTNAGG 2 cut(s) 600, 762
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoO109I RGGNCCY 1 cut(s) 499
EcoRI GAATTC 4 cut(s) 222, 342, 586, 622
EcoRII CCWGG 1 cut(s) 510
EcoT22I ATGCAT 2 cut(s) 233, 916
EgeI GGCGCC 1 cut(s) 612
EheI GGCGCC 1 cut(s) 612
FaeI CATG 4 cut(s) 108, 200, 231, 918
FatI CATG 4 cut(s) 104, 196, 227, 914
FauI CCCGC 1 cut(s) 136
FbaI TGATCA 2 cut(s) 372, 943
FblI GTMKAC 1 cut(s) 630
FokI GGATG 1 cut(s) 151
FspBI CTAG 1 cut(s) 237
GlaI GCGC 1 cut(s) 612
GsaI CCCAGC 1 cut(s) 104
GsuI CTGGAG 1 cut(s) 105
HaeII RGCGCY 1 cut(s) 614
HaeIII GGCC 3 cut(s) 118, 187, 241
HapII CCGG 3 cut(s) 34, 188, 328
HhaI GCGC 1 cut(s) 613
Hin1I GRCGYC 1 cut(s) 611
Hin1II CATG 4 cut(s) 108, 200, 231, 918
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HinfI GANTC 3 cut(s) 147, 200, 808
HpaII CCGG 3 cut(s) 34, 188, 328
HphI GGTGA 2 cut(s) 101, 582
Hpy166II GTNNAC 3 cut(s) 166, 631, 704
Hpy188I TCNGA 4 cut(s) 400, 412, 494, 544
Hpy188III TCNNGA 7 cut(s) 122, 197, 433, 461, 679, 812, 936
Hpy8I GTNNAC 3 cut(s) 166, 631, 704
HpyAV CCTTC 2 cut(s) 31, 603
HpyCH4III ACNGT 1 cut(s) 754
HpyCH4IV ACGT 2 cut(s) 353, 929
HpyCH4V TGCA 5 cut(s) 206, 231, 860, 871, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 920
HpyF3I CTNAG 3 cut(s) 411, 600, 762
HpySE526I ACGT 2 cut(s) 353, 929
Hsp92I GRCGYC 1 cut(s) 611
Hsp92II CATG 4 cut(s) 108, 200, 231, 918
HspAI GCGC 1 cut(s) 611
KasI GGCGCC 1 cut(s) 610
Ksp22I TGATCA 2 cut(s) 372, 943
Kzo9I GATC 7 cut(s) 42, 70, 322, 372, 414, 484, 943
LmnI GCTCC 1 cut(s) 52
LweI GCATC 2 cut(s) 240, 901
MaeI CTAG 1 cut(s) 237
MaeII ACGT 2 cut(s) 353, 929
MaeIII GTNAC 4 cut(s) 283, 754, 796, 925
MalI GATC 7 cut(s) 44, 72, 324, 374, 416, 486, 945
MbiI CCGCTC 1 cut(s) 55
MboI GATC 7 cut(s) 42, 70, 322, 372, 414, 484, 943
MboII GAAGA 1 cut(s) 868
MhlI GDGCHC 1 cut(s) 450
Mly113I GGCGCC 1 cut(s) 611
Mph1103I ATGCAT 2 cut(s) 233, 916
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 2 cut(s) 296, 363
MspI CCGG 3 cut(s) 34, 188, 328
MspR9I CCNGG 3 cut(s) 189, 329, 512
MvaI CCWGG 1 cut(s) 512
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 333, 920
NarI GGCGCC 1 cut(s) 611
NciI CCSGG 2 cut(s) 189, 329
NdeII GATC 7 cut(s) 42, 70, 322, 372, 414, 484, 943
NlaIII CATG 4 cut(s) 108, 200, 231, 918
NlaIV GGNNCC 4 cut(s) 7, 292, 449, 612
NmeAIII GCCGAG 1 cut(s) 606
NmuCI GTSAC 3 cut(s) 283, 754, 796
NsiI ATGCAT 2 cut(s) 233, 916
NspI RCATGY 2 cut(s) 108, 918
OliI CACNNNNGTG 1 cut(s) 296
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 2 cut(s) 118, 241
PfeI GAWTC 3 cut(s) 147, 200, 808
PluTI GGCGCC 1 cut(s) 614
Ppu21I YACGTR 2 cut(s) 354, 930
PpuMI RGGWCCY 1 cut(s) 499
PsiI TTATAA 1 cut(s) 833
Psp5II RGGWCCY 1 cut(s) 499
Psp6I CCWGG 1 cut(s) 510
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 1 cut(s) 510
PspN4I GGNNCC 4 cut(s) 7, 292, 449, 612
PspPI GGNCC 1 cut(s) 499
PspPPI RGGWCCY 1 cut(s) 499
RsaI GTAC 3 cut(s) 356, 420, 932
RsaNI GTAC 3 cut(s) 355, 419, 931
RseI CAYNNNNRTG 2 cut(s) 296, 363
SaqAI TTAA 1 cut(s) 384
Sau3AI GATC 7 cut(s) 42, 70, 322, 372, 414, 484, 943
Sau96I GGNCC 1 cut(s) 499
ScrFI CCNGG 3 cut(s) 189, 329, 512
SduI GDGCHC 1 cut(s) 450
SfaNI GCATC 2 cut(s) 240, 901
SfcI CTRYAG 2 cut(s) 112, 847
SfoI GGCGCC 1 cut(s) 612
SinI GGWCC 1 cut(s) 499
SmiMI CAYNNNNRTG 2 cut(s) 296, 363
SmlI CTYRAG 1 cut(s) 934
SmoI CTYRAG 1 cut(s) 934
SnaBI TACGTA 1 cut(s) 930
SseBI AGGCCT 2 cut(s) 118, 241
SsiI CCGC 2 cut(s) 55, 143
SspDI GGCGCC 1 cut(s) 610
SspMI CTAG 1 cut(s) 237
StuI AGGCCT 2 cut(s) 118, 241
StyD4I CCNGG 3 cut(s) 187, 327, 510
TaaI ACNGT 1 cut(s) 754
TaiI ACGT 2 cut(s) 356, 932
TaqI TCGA 2 cut(s) 27, 468
TfiI GAWTC 3 cut(s) 147, 200, 808
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 1 cut(s) 759
TseFI GTSAC 3 cut(s) 283, 754, 796
Tsp45I GTSAC 3 cut(s) 283, 754, 796
TspDTI ATGAA 1 cut(s) 708
TspGWI ACGGA 6 cut(s) 65, 354, 452, 671, 820, 851
TspRI CASTG 1 cut(s) 759
VpaK11BI GGWCC 1 cut(s) 499
XagI CCTNNNNNAGG 1 cut(s) 603
XapI RAATTY 7 cut(s) 222, 342, 389, 586, 622, 674, 728
XceI RCATGY 2 cut(s) 108, 918
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 630
XspI CTAG 1 cut(s) 237
Zsp2I ATGCAT 2 cut(s) 233, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.