MD10G1121000.v1.1

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005813
Physical Location & Seq
Forward (+)
19773657 .. 19774613
957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1121000.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGGTTCCGAGGTCTCGAAACAGGTAGAACGGAGAAAGGCGATCGCGGCGGAGAAAAAAACACTGTGTGATCTTCACCAGAGCTGTGGTGAAGAGTTTCCATCATGTGCCTACAAGCCTTCAGACAGAAAAAATTGGATGGCTGGGGTCAACCCTGATAAACTTCACATAAACAAGATCGTATGGCCGGGAACTCACGACTCTGCCACCAACAAGATTGGAGTTCCATGCATCACTAGGCCTTTTGCTCAATGCCAAAATCTCTCCATCTACCAGCAGCTCGAGAAAGGCACTAGAGTTCTCGATATTCGAATCCAGGAGGACCGAAAAGTCTGCCACGGAATCCTCCTCACTTACAACATTGATGTTGTGATCAACGACGTCAAGAAGTTTTTATCGGAAACGCAGTATGAGATCATAATACTCGAAATCCGAACTGAATTTGGACATGAAGATCCACCTGAGTTTGATAAGTACTTGGTTGATCAGCTCGGCGAGTTTTTAATCCCCCAGGACGATCATGTTTTCGACAAGACTATTTCAGAACTGCTGCCGAAGAGAGTGATATGCGTTTGGAAGCCGAGGAAGTCACCTCAGCCAAAGGCAGGGAGTCCTCTGTGGAATGCAGGGCATTTAAGGGACAACTGGATTGACACGGATTTGCCATCCACCAAATTCGAGAGCAACCTGAAGAATTTGAGTAATCAGCCGCCGGCTTCATCGAGGAAGTTCTTTTACAGGGTGGAGAACACGGTCACGCCTCAGGCCGATAACCCGGTTTTGTGTGTTAAGCCTGTGACCAGAAGGATTCATGGATTCGCGAGGCTGTTTATAACTCAGTGCTTCTCTAAAGGTATTGCGGATAGATTGCAGATCTTTTCTACAGATTTTATAGACGAGGATTTCGTGGATGCTTGCGTTGCGGTTACATATGCAAGAGTTGAAGGGAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

319

Amino Acids

36.31

Weight (kDa)

7.55

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 63 - 174 2.2e-09 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AasI GACNNNNNNGTC 1 cut(s) 329
AatII GACGTC 1 cut(s) 384
AccII CGCG 2 cut(s) 47, 821
AciI CCGC 5 cut(s) 47, 50, 710, 860, 923
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 3 cut(s) 440, 674, 694
AcuI CTGAAG 2 cut(s) 105, 710
AcyI GRCGYC 1 cut(s) 381
AdeI CACNNNGTG 1 cut(s) 68
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 1 cut(s) 944
AjnI CCWGG 2 cut(s) 315, 510
AluBI AGCT 3 cut(s) 84, 280, 490
AluI AGCT 3 cut(s) 84, 280, 490
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 3 cut(s) 185, 239, 765
ApeKI GCWGC 2 cut(s) 277, 550
ApoI RAATTY 3 cut(s) 440, 674, 694
ArsI GACNNNNNNTTYG 2 cut(s) 887, 919
AsiSI GCGATCGC 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 96
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 2 cut(s) 189, 776
AsuHPI GGTGA 3 cut(s) 68, 101, 582
AsuII TTCGAA 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 322
AxyI CCTNAGG 1 cut(s) 762
BarI GAAGNNNNNNTAC 2 cut(s) 719, 751
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 2 cut(s) 289, 537
BccI CCATC 4 cut(s) 109, 133, 275, 673
BcgI CGANNNNNNTGC 2 cut(s) 315, 349
BciT130I CCWGG 2 cut(s) 317, 512
BclI TGATCA 2 cut(s) 372, 484
BcnI CCSGG 2 cut(s) 189, 776
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 2 cut(s) 237, 294
BfmI CTRYAG 1 cut(s) 882
BglII AGATCT 1 cut(s) 873
BisI GCNGC 4 cut(s) 48, 278, 551, 710
BlsI GCNGC 4 cut(s) 49, 279, 552, 711
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 4 cut(s) 189, 317, 512, 776
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 4 cut(s) 189, 317, 512, 776
BmsI GCATC 2 cut(s) 240, 901
Bpu10I CCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 310
BpuMI CCSGG 2 cut(s) 189, 776
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 4 cut(s) 9, 337, 510, 581
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 1 cut(s) 650
Bse21I CCTNAGG 1 cut(s) 762
BseBI CCWGG 2 cut(s) 317, 512
BseDI CCNNGG 4 cut(s) 9, 337, 510, 581
BseGI GGATG 3 cut(s) 144, 665, 916
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMII CTCAG 4 cut(s) 453, 608, 776, 851
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 2 cut(s) 289, 537
BseYI CCCAGC 1 cut(s) 143
Bsh1236I CGCG 2 cut(s) 47, 821
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 3 cut(s) 187, 241, 767
BsiEI CGRYCG 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 3 cut(s) 188, 713, 776
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 1 cut(s) 605
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 653
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 3 cut(s) 187, 241, 767
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 310
Bsp143I GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 484, 517, 873
Bsp68I TCGCGA 1 cut(s) 821
BspACI CCGC 5 cut(s) 47, 50, 710, 860, 923
BspANI GGCC 3 cut(s) 187, 241, 767
BspCNI CTCAG 4 cut(s) 454, 607, 775, 850
BspFNI CGCG 2 cut(s) 47, 821
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 1 cut(s) 449
BspT104I TTCGAA 1 cut(s) 310
BspTNI GGTCTC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 1 cut(s) 650
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 4 cut(s) 9, 337, 510, 581
BssMI GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 484, 517, 873
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 2 cut(s) 317, 512
Bst4CI ACNGT 2 cut(s) 66, 754
Bst6I CTCTTC 2 cut(s) 87, 551
BstACI GRCGYC 1 cut(s) 381
BstBI TTCGAA 1 cut(s) 310
BstC8I GCNNGC 2 cut(s) 714, 916
BstDEI CTNAG 4 cut(s) 462, 594, 762, 837
BstDSI CCRYGG 1 cut(s) 337
BstF5I GGATG 3 cut(s) 144, 665, 916
BstFNI CGCG 2 cut(s) 47, 821
BstKTI GATC 9 cut(s) 45, 73, 180, 375, 417, 457, 487, 520, 876
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 484, 517, 873
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 47, 920
BstNI CCWGG 2 cut(s) 317, 512
BstSCI CCNGG 4 cut(s) 187, 315, 510, 774
BstSFI CTRYAG 1 cut(s) 882
BstUI CGCG 2 cut(s) 47, 821
BstV1I GCAGC 2 cut(s) 289, 537
BstX2I RGATCY 2 cut(s) 454, 873
BstXI CCANNNNNNTGG 1 cut(s) 86
BstYI RGATCY 2 cut(s) 454, 873
Bsu36I CCTNAGG 1 cut(s) 762
BsuRI GGCC 3 cut(s) 187, 241, 767
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 3 cut(s) 144, 665, 916
BtsIMutI CAGTG 2 cut(s) 62, 845
BtuMI TCGCGA 1 cut(s) 821
Cac8I GCNNGC 2 cut(s) 714, 916
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 1 cut(s) 475
CviAII CATG 6 cut(s) 105, 228, 449, 521, 812, 954
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 4 cut(s) 462, 594, 762, 837
DpnI GATC 9 cut(s) 44, 72, 179, 374, 416, 456, 486, 519, 875
DpnII GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 484, 517, 873
DraIII CACNNNGTG 1 cut(s) 68
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 185
Eam1104I CTCTTC 2 cut(s) 87, 551
EarI CTCTTC 2 cut(s) 87, 551
EciI GGCGGA 1 cut(s) 65
Eco147I AGGCCT 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 105, 710
Eco81I CCTNAGG 1 cut(s) 762
Eco88I CYCGRG 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 315, 510
EcoT22I ATGCAT 1 cut(s) 233
FaeI CATG 6 cut(s) 108, 231, 452, 524, 815, 957
FaqI GGGAC 1 cut(s) 653
FatI CATG 6 cut(s) 104, 227, 448, 520, 811, 953
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 2 cut(s) 372, 484
Fnu4HI GCNGC 4 cut(s) 48, 278, 551, 710
FokI GGATG 3 cut(s) 151, 652, 923
Fsp4HI GCNGC 4 cut(s) 48, 278, 551, 710
FspBI CTAG 2 cut(s) 237, 294
GluI GCNGC 4 cut(s) 48, 278, 551, 710
GsaI CCCAGC 1 cut(s) 147
HaeIII GGCC 3 cut(s) 187, 241, 767
HapII CCGG 3 cut(s) 188, 713, 776
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 6 cut(s) 108, 231, 452, 524, 815, 957
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 6 cut(s) 200, 312, 342, 610, 808, 816
HpaII CCGG 3 cut(s) 188, 713, 776
HphI GGTGA 3 cut(s) 68, 101, 582
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 5 cut(s) 10, 124, 400, 434, 544
Hpy188III TCNNGA 7 cut(s) 16, 197, 283, 302, 385, 679, 820
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 3 cut(s) 129, 798, 938
HpyCH4III ACNGT 2 cut(s) 66, 754
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 4 cut(s) 231, 626, 871, 935
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 920
HpyF3I CTNAG 4 cut(s) 462, 594, 762, 837
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 6 cut(s) 108, 231, 452, 524, 815, 957
KroI GCCGGC 1 cut(s) 712
KroNI GCCGGC 1 cut(s) 714
Ksp22I TGATCA 2 cut(s) 372, 484
Kzo9I GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 484, 517, 873
Lsp1109I GCAGC 2 cut(s) 289, 537
LweI GCATC 2 cut(s) 240, 901
MaeI CTAG 2 cut(s) 237, 294
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 4 cut(s) 588, 754, 796, 925
MalI GATC 9 cut(s) 44, 72, 179, 374, 416, 456, 486, 519, 875
MboI GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 484, 517, 873
MboII GAAGA 5 cut(s) 65, 104, 464, 568, 703
MflI RGATCY 2 cut(s) 454, 873
MluCI AATT 4 cut(s) 133, 440, 674, 694
MlyI GAGTC 2 cut(s) 194, 619
Mph1103I ATGCAT 1 cut(s) 233
MroNI GCCGGC 1 cut(s) 712
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 3 cut(s) 503, 635, 789
MspI CCGG 3 cut(s) 188, 713, 776
MspR9I CCNGG 4 cut(s) 189, 317, 512, 776
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 2 cut(s) 317, 512
MvnI CGCG 2 cut(s) 47, 821
MwoI GCNNNNNNNGC 2 cut(s) 47, 920
NaeI GCCGGC 1 cut(s) 714
NciI CCSGG 2 cut(s) 189, 776
NdeI CATATG 1 cut(s) 931
NdeII GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 484, 517, 873
NgoMIV GCCGGC 1 cut(s) 712
NlaIII CATG 6 cut(s) 108, 231, 452, 524, 815, 957
NlaIV GGNNCC 1 cut(s) 7
NmeAIII GCCGAG 2 cut(s) 471, 606
NmuCI GTSAC 3 cut(s) 588, 754, 796
NruI TCGCGA 1 cut(s) 821
NsiI ATGCAT 1 cut(s) 233
NspV TTCGAA 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 281
PceI AGGCCT 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PctI GAATGC 1 cut(s) 628
PdiI GCCGGC 1 cut(s) 714
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 4 cut(s) 312, 342, 808, 816
PfoI TCCNGGA 1 cut(s) 315
PkrI GCNGC 4 cut(s) 49, 279, 552, 711
Ple19I CGATCG 1 cut(s) 45
PleI GAGTC 2 cut(s) 194, 618
PpsI GAGTC 2 cut(s) 194, 618
PsiI TTATAA 1 cut(s) 833
Psp6I CCWGG 2 cut(s) 315, 510
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 315, 510
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 322
PsuI RGATCY 2 cut(s) 454, 873
PvuI CGATCG 1 cut(s) 45
RgaI GCGATCGC 1 cut(s) 45
RruI TCGCGA 1 cut(s) 821
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SaqAI TTAA 3 cut(s) 503, 635, 789
SatI GCNGC 4 cut(s) 48, 278, 551, 710
Sau3AI GATC 9 cut(s) 42, 70, 177, 372, 414, 454, 484, 517, 873
Sau96I GGNCC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 2 cut(s) 194, 619
ScrFI CCNGG 4 cut(s) 189, 317, 512, 776
SfaAI GCGATCGC 1 cut(s) 45
SfaNI GCATC 2 cut(s) 240, 901
SfcI CTRYAG 1 cut(s) 882
Sfr274I CTCGAG 1 cut(s) 281
SfuI TTCGAA 1 cut(s) 310
SgfI GCGATCGC 1 cut(s) 45
SinI GGWCC 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 4 cut(s) 133, 440, 674, 694
SseBI AGGCCT 1 cut(s) 241
SsiI CCGC 5 cut(s) 47, 50, 710, 860, 923
SspMI CTAG 2 cut(s) 237, 294
StuI AGGCCT 1 cut(s) 241
StyD4I CCNGG 4 cut(s) 187, 315, 510, 774
TaaI ACNGT 2 cut(s) 66, 754
TaiI ACGT 1 cut(s) 384
TaqI TCGA 8 cut(s) 17, 282, 303, 310, 426, 528, 678, 722
TaqII GACCGA 1 cut(s) 339
TasI AATT 4 cut(s) 133, 440, 674, 694
TatI WGTACW 1 cut(s) 474
TauI GCSGC 2 cut(s) 50, 712
TfiI GAWTC 4 cut(s) 312, 342, 808, 816
Tru1I TTAA 3 cut(s) 503, 635, 789
Tru9I TTAA 3 cut(s) 503, 635, 789
TscAI CASTG 2 cut(s) 69, 845
TseFI GTSAC 3 cut(s) 588, 754, 796
TseI GCWGC 2 cut(s) 277, 550
Tsp45I GTSAC 3 cut(s) 588, 754, 796
TspDTI ATGAA 3 cut(s) 465, 708, 800
TspGWI ACGGA 3 cut(s) 46, 354, 671
TspRI CASTG 2 cut(s) 69, 845
VpaK11BI GGWCC 1 cut(s) 322
XapI RAATTY 3 cut(s) 440, 674, 694
XhoI CTCGAG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 2 cut(s) 237, 294
ZraI GACGTC 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 476
Zsp2I ATGCAT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.