MD10G1118000.v1.1

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005813
Physical Location & Seq
Forward (+)
19527538 .. 19530093
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1118000.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGGTGTCATGGATAAACCATTAGTCATAACAATGGAAGAAGATATTCGTCATCATGACTATCTTAGGAGAGCTCTTTTACTGTGTGGGGATCGCATTCGCATGGAATTGGATGACACCAAACACAATGATGTATTTCTCGAACGCCGGAAGGCGATCTCAACGGAGCGGAAAGTGCTACGTGATCTCCACCAAAGCGTTGGTGAGGACTTCCCAGCATGTGCCTACAGGCCTCCAGACAGGAAAAATTGGATAGCGGGGATCAACCCGGAAAAAGTTCACGTAAACAAAATTGTATGGCCAGGAACTCACGACTCTGCAACCAACAAGATTGGAATTCCATGCATCACTAGGCCTTTTGGACAATGCCAAACAATGTCTATATACAAACAGCTTGTCACAGGCACTAGAGTCCTTGACATTCGGGTTCAGAAAGATTGTCATGTGTGCCACGGAATTCTCGTCACATACCACATTGATGTCGTGATAAAGGACATTAAAAAATTTCTGTCAGAAACAGAGTCTGAGATCATACTTCTCGAAATCAGGACGGAATATGGGCACCAGGACCCTCCTGATTTCGATAAGTATTTGGTAGAACAGCTCGGAGAGGTTCTAATCAACCAGAACGACAATGTGTTCAACAAGACTATTTCAGAGCTGTTACCGAAGCGAATAATATGTGTGTGGAAGCCAAGGAATTCACCTCCCCCTAAGGCAGGGGGGCCTCTGTGGAATTCTATCTACCTGAAGGATGATTGGATTGATACGGATTTGCCATCAACAAAATTTGAGAACAATTTGAAGTGTTTGAGTGAACAACCTCCAATTTCATCAAGGAAATTTTTTTACAGGGTGGAGAACACAGTGACACCTCAGGCTGATAACCTTGTGGTATGTGTTAGGCCTGTGACCAGGCGGATTCATGAGTTTGCACGGTTGTTTATAACGGAGTGCTTCTCTAGAGGTATTGCAGATAGGTTGCAAATCTTCTCTACTGATTTTATAGATGAGGATTTTGTTGATGCATGTGTTGCTGTTACATACTCAAGAATTGATCATGGGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

358

Amino Acids

41.26

Weight (kDa)

6.64

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 101 - 214 2.5e-10 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 947
AccB1I GGYRCC 1 cut(s) 561
AccBSI CCGCTC 1 cut(s) 169
AciI CCGC 3 cut(s) 169, 257, 919
AclWI GGATC 2 cut(s) 99, 269
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 7 cut(s) 336, 456, 503, 700, 736, 788, 842
AcuI CTGAAG 1 cut(s) 770
AfiI CCNNNNNNNGG 1 cut(s) 719
AgsI TTSAA 2 cut(s) 643, 805
AjnI CCWGG 3 cut(s) 301, 564, 914
AluBI AGCT 4 cut(s) 74, 394, 604, 661
AluI AGCT 4 cut(s) 74, 394, 604, 661
Alw21I GWGCWC 1 cut(s) 76
AlwI GGATC 2 cut(s) 99, 269
AlwNI CAGNNNCTG 1 cut(s) 524
AoxI GGCC 5 cut(s) 230, 299, 353, 725, 905
ApoI RAATTY 7 cut(s) 336, 456, 503, 700, 736, 788, 842
ArsI GACNNNNNNTTYG 2 cut(s) 381, 413
Asp700I GAANNNNTTC 2 cut(s) 45, 276
AspS9I GGNCC 2 cut(s) 568, 725
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 2 cut(s) 215, 696
AvaII GGWCC 1 cut(s) 568
AxyI CCTNAGG 2 cut(s) 714, 876
BaeGI GKGCMC 1 cut(s) 564
BalI TGGCCA 1 cut(s) 301
BanI GGYRCC 1 cut(s) 561
BanII GRGCYC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 76
BccI CCATC 1 cut(s) 787
BciT130I CCWGG 3 cut(s) 303, 566, 916
BclI TGATCA 1 cut(s) 1057
BcnI CCSGG 1 cut(s) 269
BfaI CTAG 3 cut(s) 351, 408, 963
BfmI CTRYAG 1 cut(s) 226
Bme1390I CCNGG 4 cut(s) 269, 303, 566, 916
Bme18I GGWCC 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 568, 725
BmiI GGNNCC 3 cut(s) 563, 570, 726
BmrFI CCNGG 4 cut(s) 269, 303, 566, 916
BmsI GCATC 2 cut(s) 354, 1015
BplI GAGNNNNNCTC 2 cut(s) 944, 976
BpmI CTGGAG 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 1033
BpuMI CCSGG 1 cut(s) 269
BsaAI YACGTR 2 cut(s) 182, 283
BsaJI CCNNGG 2 cut(s) 451, 695
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse21I CCTNAGG 2 cut(s) 714, 876
BseBI CCWGG 3 cut(s) 303, 566, 916
BseDI CCNNGG 2 cut(s) 451, 695
BseGI GGATG 2 cut(s) 118, 760
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMII CTCAG 2 cut(s) 516, 890
BseSI GKGCMC 1 cut(s) 564
BseYI CCCAGC 1 cut(s) 214
BshFI GGCC 5 cut(s) 232, 301, 355, 727, 907
BshNI GGYRCC 1 cut(s) 561
BsiHKAI GWGCWC 1 cut(s) 76
BsiSI CCGG 2 cut(s) 148, 269
BslI CCNNNNNNNGG 1 cut(s) 719
BsmI GAATGC 1 cut(s) 96
BsnI GGCC 5 cut(s) 232, 301, 355, 727, 907
Bsp1286I GDGCHC 2 cut(s) 76, 564
Bsp143I GATC 6 cut(s) 91, 156, 184, 261, 528, 1057
BspACI CCGC 3 cut(s) 169, 257, 919
BspANI GGCC 5 cut(s) 232, 301, 355, 727, 907
BspCNI CTCAG 2 cut(s) 517, 889
BspHI TCATGA 2 cut(s) 55, 925
BspLI GGNNCC 3 cut(s) 563, 570, 726
BspPI GGATC 2 cut(s) 99, 269
BspT107I GGYRCC 1 cut(s) 561
BsrBI CCGCTC 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 451, 695
BssMI GATC 6 cut(s) 91, 156, 184, 261, 528, 1057
BssT1I CCWWGG 1 cut(s) 695
Bst2UI CCWGG 3 cut(s) 303, 566, 916
Bst4CI ACNGT 3 cut(s) 84, 868, 939
BstAPI GCANNNNNTGC 1 cut(s) 1034
BstBAI YACGTR 2 cut(s) 182, 283
BstDEI CTNAG 4 cut(s) 65, 525, 714, 876
BstDSI CCRYGG 1 cut(s) 451
BstENI CCTNNNNNAGG 1 cut(s) 717
BstF5I GGATG 2 cut(s) 118, 760
BstKTI GATC 6 cut(s) 94, 159, 187, 264, 531, 1060
BstMBI GATC 6 cut(s) 91, 156, 184, 261, 528, 1057
BstMWI GCNNNNNNNGC 2 cut(s) 175, 1034
BstNI CCWGG 3 cut(s) 303, 566, 916
BstNSI RCATGY 2 cut(s) 222, 1032
BstSCI CCNGG 4 cut(s) 267, 301, 564, 914
BstSFI CTRYAG 1 cut(s) 226
BstSLI GKGCMC 1 cut(s) 564
BstXI CCANNNNNNTGG 1 cut(s) 200
Bsu36I CCTNAGG 2 cut(s) 714, 876
BsuRI GGCC 5 cut(s) 232, 301, 355, 727, 907
BtgI CCRYGG 1 cut(s) 451
BtsCI GGATG 2 cut(s) 118, 760
BtsIMutI CAGTG 1 cut(s) 873
CaiI CAGNNNCTG 1 cut(s) 524
CciI TCATGA 2 cut(s) 55, 925
Cfr13I GGNCC 2 cut(s) 568, 725
CviAII CATG 9 cut(s) 10, 56, 103, 219, 342, 443, 926, 1029, 1061
DdeI CTNAG 4 cut(s) 65, 525, 714, 876
DpnI GATC 6 cut(s) 93, 158, 186, 263, 530, 1059
DpnII GATC 6 cut(s) 91, 156, 184, 261, 528, 1057
EaeI YGGCCR 1 cut(s) 299
EciI GGCGGA 1 cut(s) 934
Ecl136II GAGCTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 695
Eco147I AGGCCT 3 cut(s) 232, 355, 907
Eco24I GRGCYC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 568
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 770
Eco81I CCTNAGG 2 cut(s) 714, 876
EcoICRI GAGCTC 1 cut(s) 74
EcoNI CCTNNNNNAGG 1 cut(s) 717
EcoO109I RGGNCCY 2 cut(s) 568, 725
EcoRI GAATTC 4 cut(s) 336, 456, 700, 736
EcoRII CCWGG 3 cut(s) 301, 564, 914
EcoT14I CCWWGG 1 cut(s) 695
EcoT22I ATGCAT 2 cut(s) 347, 1030
EcoT38I GRGCYC 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 695
FaeI CATG 9 cut(s) 13, 59, 106, 222, 345, 446, 929, 1032, 1064
FatI CATG 9 cut(s) 9, 55, 102, 218, 341, 442, 925, 1028, 1060
FauI CCCGC 1 cut(s) 250
FbaI TGATCA 1 cut(s) 1057
FokI GGATG 2 cut(s) 125, 767
FriOI GRGCYC 1 cut(s) 76
FspBI CTAG 3 cut(s) 351, 408, 963
GsaI CCCAGC 1 cut(s) 218
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 5 cut(s) 232, 301, 355, 727, 907
HapII CCGG 2 cut(s) 148, 269
Hin1II CATG 9 cut(s) 13, 59, 106, 222, 345, 446, 929, 1032, 1064
HinfI GANTC 4 cut(s) 314, 411, 521, 922
HpaII CCGG 2 cut(s) 148, 269
HphI GGTGA 2 cut(s) 215, 696
Hpy166II GTNNAC 3 cut(s) 280, 286, 818
Hpy188I TCNGA 5 cut(s) 432, 514, 526, 608, 658
Hpy8I GTNNAC 3 cut(s) 280, 286, 818
HpyAV CCTTC 2 cut(s) 145, 745
HpyCH4III ACNGT 3 cut(s) 84, 868, 939
HpyCH4IV ACGT 2 cut(s) 181, 282
HpyCH4V TGCA 6 cut(s) 320, 345, 935, 974, 985, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 1034
HpyF3I CTNAG 4 cut(s) 65, 525, 714, 876
HpySE526I ACGT 2 cut(s) 181, 282
Hsp92II CATG 9 cut(s) 13, 59, 106, 222, 345, 446, 929, 1032, 1064
Ksp22I TGATCA 1 cut(s) 1057
Kzo9I GATC 6 cut(s) 91, 156, 184, 261, 528, 1057
LmnI GCTCC 1 cut(s) 166
LweI GCATC 2 cut(s) 354, 1015
MaeI CTAG 3 cut(s) 351, 408, 963
MaeII ACGT 2 cut(s) 181, 282
MaeIII GTNAC 6 cut(s) 397, 463, 663, 868, 910, 1039
MalI GATC 6 cut(s) 93, 158, 186, 263, 530, 1059
MbiI CCGCTC 1 cut(s) 169
MboI GATC 6 cut(s) 91, 156, 184, 261, 528, 1057
MboII GAAGA 3 cut(s) 50, 53, 982
MhlI GDGCHC 2 cut(s) 76, 564
MlsI TGGCCA 1 cut(s) 301
MluNI TGGCCA 1 cut(s) 301
MlyI GAGTC 3 cut(s) 308, 420, 530
Mox20I TGGCCA 1 cut(s) 301
Mph1103I ATGCAT 2 cut(s) 347, 1030
MroXI GAANNNNTTC 2 cut(s) 45, 276
MscI TGGCCA 1 cut(s) 301
MseI TTAA 1 cut(s) 498
MslI CAYNNNNRTG 4 cut(s) 32, 101, 129, 477
Msp20I TGGCCA 1 cut(s) 301
MspI CCGG 2 cut(s) 148, 269
MspR9I CCNGG 4 cut(s) 269, 303, 566, 916
Mva1269I GAATGC 1 cut(s) 96
MvaI CCWGG 3 cut(s) 303, 566, 916
MwoI GCNNNNNNNGC 2 cut(s) 175, 1034
NciI CCSGG 1 cut(s) 269
NdeII GATC 6 cut(s) 91, 156, 184, 261, 528, 1057
NlaIII CATG 9 cut(s) 13, 59, 106, 222, 345, 446, 929, 1032, 1064
NlaIV GGNNCC 3 cut(s) 563, 570, 726
NmuCI GTSAC 4 cut(s) 397, 463, 868, 910
NsiI ATGCAT 2 cut(s) 347, 1030
NspI RCATGY 2 cut(s) 222, 1032
PagI TCATGA 2 cut(s) 55, 925
PceI AGGCCT 3 cut(s) 232, 355, 907
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PctI GAATGC 1 cut(s) 96
PdmI GAANNNNTTC 2 cut(s) 45, 276
PfeI GAWTC 1 cut(s) 922
PleI GAGTC 3 cut(s) 308, 419, 529
PpsI GAGTC 3 cut(s) 308, 419, 529
Ppu21I YACGTR 2 cut(s) 182, 283
PpuMI RGGWCCY 1 cut(s) 568
PsiI TTATAA 1 cut(s) 947
Psp124BI GAGCTC 1 cut(s) 76
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 3 cut(s) 301, 564, 914
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 3 cut(s) 301, 564, 914
PspN4I GGNNCC 3 cut(s) 563, 570, 726
PspPI GGNCC 2 cut(s) 568, 725
PspPPI RGGWCCY 1 cut(s) 568
PstNI CAGNNNCTG 1 cut(s) 524
RseI CAYNNNNRTG 4 cut(s) 32, 101, 129, 477
SacI GAGCTC 1 cut(s) 76
SaqAI TTAA 1 cut(s) 498
Sau3AI GATC 6 cut(s) 91, 156, 184, 261, 528, 1057
Sau96I GGNCC 2 cut(s) 568, 725
SchI GAGTC 3 cut(s) 308, 420, 530
ScrFI CCNGG 4 cut(s) 269, 303, 566, 916
SduI GDGCHC 2 cut(s) 76, 564
SfaNI GCATC 2 cut(s) 354, 1015
SfcI CTRYAG 1 cut(s) 226
SinI GGWCC 1 cut(s) 568
SmiMI CAYNNNNRTG 4 cut(s) 32, 101, 129, 477
SmlI CTYRAG 1 cut(s) 1048
SmoI CTYRAG 1 cut(s) 1048
SseBI AGGCCT 3 cut(s) 232, 355, 907
SsiI CCGC 3 cut(s) 169, 257, 919
SspMI CTAG 3 cut(s) 351, 408, 963
SstI GAGCTC 1 cut(s) 76
StuI AGGCCT 3 cut(s) 232, 355, 907
StyD4I CCNGG 4 cut(s) 267, 301, 564, 914
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 3 cut(s) 84, 868, 939
TaiI ACGT 2 cut(s) 184, 285
TaqI TCGA 3 cut(s) 141, 540, 582
TfiI GAWTC 1 cut(s) 922
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 1 cut(s) 873
TseFI GTSAC 4 cut(s) 397, 463, 868, 910
Tsp45I GTSAC 4 cut(s) 397, 463, 868, 910
TspDTI ATGAA 2 cut(s) 822, 914
TspGWI ACGGA 5 cut(s) 179, 468, 566, 785, 965
TspRI CASTG 1 cut(s) 873
VpaK11BI GGWCC 1 cut(s) 568
XagI CCTNNNNNAGG 1 cut(s) 717
XapI RAATTY 7 cut(s) 336, 456, 503, 700, 736, 788, 842
XbaI TCTAGA 1 cut(s) 962
XceI RCATGY 2 cut(s) 222, 1032
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmnI GAANNNNTTC 2 cut(s) 45, 276
XspI CTAG 3 cut(s) 351, 408, 963
Zsp2I ATGCAT 2 cut(s) 347, 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.