RchiOBHm_Chr6g0245281

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005813
Physical Location & Seq
Reverse (-)
1155034 .. 1156065
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21983

Sequence Viewer

Length: 957 bp
ATGGGATCCGGGGTCTCTAGACAGGTAGAAAGGCGCAAGGCACTCACAGTGGAAAGGAGAAAGTTATATGATCTTCATCAAAGCTGCGGTGAAGACTTTCCAGCTTGTGCATTCAGGCCTTCAGATAGAAAAAACTGGATGGATGACCTCCATCCTGAGAAACTTCAGATATGCAAGATTGTTTGGCCCGGAACTCATGATTCTGCAACAGATAGGATTGGAATCCCATTGGTCACCAGGCCTTTTGCACAATGCCAAACTCTATCTGTCTATCGCCAGCTTGTGCTAGGCTGCAGGGTTCTTGACATTCGAGTTCAGAAGGATCGCCGAGTCTGTCATGGGATTCTTGTCTCGTATAATGTTGATGTTGTGATCAATGATATAAAGAAGTTCCTGTCTGAAACACATTCAGAGATCATAATCCTTGAAGTCCGTACCGAGTATGGACATGACGACCCTCCTGAATTCGACAAATATTTGGTAGATCACCTCGGACAATTCCTGATCCACCACAATGACAATGTGTTTCACAAGACTATTTCGGAAGTGTTGCCAAAGAGAATAATATGCATATGGAAGCCAAGGAATTCACCTAACCCCAAAAATGGGAGCCCTCTGTGGAATTCTGGTCATTTGAAGGATGATTGGATTAACACGGATCTGCCATCAACCAAGTTTGAAAGCAACCTCAAGTATTTGAGTGAGCAGCCACCAGTTTCCGCAAGGAAATTTTTTTACAGGGTGGAGAACACAGTGACACCGCGGCCTGATAACCCTGTTGTTTGTGTAGGGCCTGTGACAAAGCGGATTCATGAATTTGCAAGATTGTTCATAACTCAGTGCTTCTCTCGAGGCATTGCAGATAGGTTGCAAATCTTCTCCACAGATTATATAGACGAGGATTTTGTTGATGCCTGTGTTGCACTTACATATGCAAGAGCTGAAGGGAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

318

Amino Acids

36.67

Weight (kDa)

8.28

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 63 - 175 1.5e-09 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 763
AciI CCGC 5 cut(s) 87, 720, 761, 763, 805
AclWI GGATC 4 cut(s) 13, 330, 499, 666
AcsI RAATTY 5 cut(s) 464, 586, 622, 728, 815
AcuI CTGAAG 2 cut(s) 105, 149
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 2 cut(s) 605, 606
AgsI TTSAA 3 cut(s) 428, 637, 680
AjnI CCWGG 1 cut(s) 236
AluBI AGCT 5 cut(s) 84, 104, 280, 941, 953
AluI AGCT 5 cut(s) 84, 104, 280, 941, 953
Alw26I GTCTC 2 cut(s) 19, 355
AlwI GGATC 4 cut(s) 13, 330, 499, 666
Ama87I CYCGRG 1 cut(s) 849
AoxI GGCC 5 cut(s) 116, 185, 239, 764, 791
ApeKI GCWGC 3 cut(s) 84, 291, 706
ApoI RAATTY 5 cut(s) 464, 586, 622, 728, 815
ArsI GACNNNNNNTTYG 2 cut(s) 887, 919
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 186, 791
AsuC2I CCSGG 2 cut(s) 10, 189
AsuHPI GGTGA 4 cut(s) 101, 226, 479, 582
AvaI CYCGRG 1 cut(s) 849
BamHI GGATCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 614
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 3 cut(s) 71, 278, 718
BccI CCATC 3 cut(s) 133, 159, 673
BciT130I CCWGG 1 cut(s) 238
BclI TGATCA 1 cut(s) 372
BcnI CCSGG 2 cut(s) 10, 189
BcoDI GTCTC 2 cut(s) 19, 355
BfaI CTAG 2 cut(s) 18, 287
BfmI CTRYAG 1 cut(s) 292
BisI GCNGC 4 cut(s) 85, 292, 707, 764
BlsI GCNGC 4 cut(s) 86, 293, 708, 765
Bme1390I CCNGG 3 cut(s) 10, 189, 238
BmeT110I CYCGRG 1 cut(s) 849
BmgT120I GGNCC 2 cut(s) 186, 791
BmiI GGNNCC 2 cut(s) 7, 611
BmrFI CCNGG 3 cut(s) 10, 189, 238
BmsI GCATC 1 cut(s) 901
BpiI GAAGAC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 2 cut(s) 10, 189
BsaBI GATNNNNATC 3 cut(s) 75, 221, 419
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 4 cut(s) 9, 490, 581, 761
Bsc4I CCNNNNNNNGG 2 cut(s) 605, 606
Bse1I ACTGG 2 cut(s) 140, 713
Bse3DI GCAATG 1 cut(s) 855
Bse8I GATNNNNATC 3 cut(s) 75, 221, 419
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 4 cut(s) 9, 490, 581, 761
BseGI GGATG 4 cut(s) 144, 148, 151, 646
BseJI GATNNNNATC 3 cut(s) 75, 221, 419
BseLI CCNNNNNNNGG 2 cut(s) 605, 606
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 2 cut(s) 147, 851
BseNI ACTGG 2 cut(s) 140, 713
BseXI GCAGC 3 cut(s) 71, 278, 718
Bsh1236I CGCG 1 cut(s) 763
BshFI GGCC 5 cut(s) 118, 187, 241, 766, 793
BsiHKCI CYCGRG 1 cut(s) 849
BsiSI CCGG 2 cut(s) 9, 189
BslI CCNNNNNNNGG 2 cut(s) 605, 606
BsmAI GTCTC 2 cut(s) 19, 355
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 5 cut(s) 118, 187, 241, 766, 793
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 849
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 8 cut(s) 5, 70, 322, 372, 414, 484, 504, 658
BspACI CCGC 5 cut(s) 87, 720, 761, 763, 805
BspANI GGCC 5 cut(s) 118, 187, 241, 766, 793
BspCNI CTCAG 2 cut(s) 148, 850
BspFNI CGCG 1 cut(s) 763
BspHI TCATGA 2 cut(s) 196, 811
BspLI GGNNCC 2 cut(s) 7, 611
BspMAI CTGCAG 1 cut(s) 296
BspPI GGATC 4 cut(s) 13, 330, 499, 666
BspTNI GGTCTC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 855
BsrI ACTGG 2 cut(s) 140, 713
BssECI CCNNGG 4 cut(s) 9, 490, 581, 761
BssMI GATC 8 cut(s) 5, 70, 322, 372, 414, 484, 504, 658
BssT1I CCWWGG 1 cut(s) 581
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 49, 754
BstC8I GCNNGC 1 cut(s) 278
BstDEI CTNAG 2 cut(s) 156, 837
BstDSI CCRYGG 1 cut(s) 761
BstEII GGTNACC 1 cut(s) 232
BstF5I GGATG 4 cut(s) 144, 148, 151, 646
BstFNI CGCG 1 cut(s) 763
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 8 cut(s) 8, 73, 325, 375, 417, 487, 507, 661
BstMAI GTCTC 2 cut(s) 19, 355
BstMBI GATC 8 cut(s) 5, 70, 322, 372, 414, 484, 504, 658
BstMWI GCNNNNNNNGC 1 cut(s) 920
BstNI CCWGG 1 cut(s) 238
BstPI GGTNACC 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 8, 187, 236
BstSFI CTRYAG 1 cut(s) 292
BstUI CGCG 1 cut(s) 763
BstV1I GCAGC 3 cut(s) 71, 278, 718
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 2 cut(s) 5, 658
BstYI RGATCY 2 cut(s) 5, 658
BsuRI GGCC 5 cut(s) 118, 187, 241, 766, 793
BtgI CCRYGG 1 cut(s) 761
BtsCI GGATG 4 cut(s) 144, 148, 151, 646
BtsIMutI CAGTG 3 cut(s) 54, 759, 845
Cac8I GCNNGC 1 cut(s) 278
CciI TCATGA 2 cut(s) 196, 811
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 186, 791
Cfr42I CCGCGG 1 cut(s) 764
Csp6I GTAC 1 cut(s) 435
CviAII CATG 4 cut(s) 197, 338, 449, 812
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 2 cut(s) 156, 837
DpnI GATC 8 cut(s) 7, 72, 324, 374, 416, 486, 506, 660
DpnII GATC 8 cut(s) 5, 70, 322, 372, 414, 484, 504, 658
Eco130I CCWWGG 1 cut(s) 581
Eco147I AGGCCT 2 cut(s) 118, 241
Eco24I GRGCYC 1 cut(s) 614
Eco31I GGTCTC 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 105, 149
Eco88I CYCGRG 1 cut(s) 849
Eco91I GGTNACC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 791
EcoO65I GGTNACC 1 cut(s) 232
EcoRI GAATTC 3 cut(s) 464, 586, 622
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 581
EcoT22I ATGCAT 1 cut(s) 572
EcoT38I GRGCYC 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 581
FaeI CATG 4 cut(s) 200, 341, 452, 815
FatI CATG 4 cut(s) 196, 337, 448, 811
FauNDI CATATG 2 cut(s) 572, 931
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 4 cut(s) 85, 292, 707, 764
FokI GGATG 4 cut(s) 138, 151, 155, 653
FriOI GRGCYC 1 cut(s) 614
Fsp4HI GCNGC 4 cut(s) 85, 292, 707, 764
FspBI CTAG 2 cut(s) 18, 287
GlaI GCGC 1 cut(s) 35
GluI GCNGC 4 cut(s) 85, 292, 707, 764
HaeIII GGCC 5 cut(s) 118, 187, 241, 766, 793
HapII CCGG 2 cut(s) 9, 189
HhaI GCGC 1 cut(s) 36
Hin1II CATG 4 cut(s) 200, 341, 452, 815
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 951
HinfI GANTC 5 cut(s) 200, 222, 330, 343, 808
HpaII CCGG 2 cut(s) 9, 189
HphI GGTGA 4 cut(s) 101, 226, 479, 582
Hpy188I TCNGA 7 cut(s) 124, 168, 318, 400, 412, 494, 544
Hpy188III TCNNGA 8 cut(s) 18, 155, 197, 302, 461, 502, 812, 849
HpyAV CCTTC 4 cut(s) 129, 313, 631, 938
HpyCH4III ACNGT 2 cut(s) 49, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 920
HpyF3I CTNAG 2 cut(s) 156, 837
Hsp92II CATG 4 cut(s) 200, 341, 452, 815
HspAI GCGC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 372
KspI CCGCGG 1 cut(s) 764
Kzo9I GATC 8 cut(s) 5, 70, 322, 372, 414, 484, 504, 658
LmnI GCTCC 1 cut(s) 609
Lsp1109I GCAGC 3 cut(s) 71, 278, 718
LweI GCATC 1 cut(s) 901
MaeI CTAG 2 cut(s) 18, 287
MaeIII GTNAC 3 cut(s) 232, 754, 796
MalI GATC 8 cut(s) 7, 72, 324, 374, 416, 486, 506, 660
MboI GATC 8 cut(s) 5, 70, 322, 372, 414, 484, 504, 658
MboII GAAGA 3 cut(s) 65, 104, 868
MflI RGATCY 2 cut(s) 5, 658
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 6 cut(s) 464, 497, 586, 622, 728, 815
MlyI GAGTC 1 cut(s) 339
MnlI CCTC 7 cut(s) 158, 468, 500, 624, 698, 845, 892
Mph1103I ATGCAT 1 cut(s) 572
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 1 cut(s) 651
MslI CAYNNNNRTG 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 763
MspI CCGG 2 cut(s) 9, 189
MspR9I CCNGG 3 cut(s) 10, 189, 238
Mva1269I GAATGC 1 cut(s) 110
MvaI CCWGG 1 cut(s) 238
MvnI CGCG 1 cut(s) 763
MwoI GCNNNNNNNGC 1 cut(s) 920
NciI CCSGG 2 cut(s) 10, 189
NdeI CATATG 2 cut(s) 572, 931
NdeII GATC 8 cut(s) 5, 70, 322, 372, 414, 484, 504, 658
NlaIII CATG 4 cut(s) 200, 341, 452, 815
NlaIV GGNNCC 2 cut(s) 7, 611
NmeAIII GCCGAG 1 cut(s) 353
NmuCI GTSAC 3 cut(s) 232, 754, 796
NsiI ATGCAT 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 849
PagI TCATGA 2 cut(s) 196, 811
PceI AGGCCT 2 cut(s) 118, 241
PctI GAATGC 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 4 cut(s) 200, 222, 343, 808
PkrI GCNGC 4 cut(s) 86, 293, 708, 765
PleI GAGTC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 236
PspEI GGTNACC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 7, 611
PspPI GGNCC 2 cut(s) 186, 791
PstI CTGCAG 1 cut(s) 296
PsuI RGATCY 2 cut(s) 5, 658
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 513
SacII CCGCGG 1 cut(s) 764
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 4 cut(s) 85, 292, 707, 764
Sau3AI GATC 8 cut(s) 5, 70, 322, 372, 414, 484, 504, 658
Sau96I GGNCC 2 cut(s) 186, 791
SchI GAGTC 1 cut(s) 339
ScrFI CCNGG 3 cut(s) 10, 189, 238
SduI GDGCHC 1 cut(s) 614
SfaNI GCATC 1 cut(s) 901
SfcI CTRYAG 1 cut(s) 292
Sfr274I CTCGAG 1 cut(s) 849
Sfr303I CCGCGG 1 cut(s) 764
SgrBI CCGCGG 1 cut(s) 764
SlaI CTCGAG 1 cut(s) 849
SmiMI CAYNNNNRTG 1 cut(s) 513
SmlI CTYRAG 2 cut(s) 689, 849
SmoI CTYRAG 2 cut(s) 689, 849
Sse9I AATT 6 cut(s) 464, 497, 586, 622, 728, 815
SseBI AGGCCT 2 cut(s) 118, 241
SsiI CCGC 5 cut(s) 87, 720, 761, 763, 805
SspI AATATT 1 cut(s) 476
SspMI CTAG 2 cut(s) 18, 287
StuI AGGCCT 2 cut(s) 118, 241
StyD4I CCNGG 3 cut(s) 8, 187, 236
StyI CCWWGG 1 cut(s) 581
TaaI ACNGT 2 cut(s) 49, 754
TaqI TCGA 3 cut(s) 310, 468, 850
TasI AATT 6 cut(s) 464, 497, 586, 622, 728, 815
TauI GCSGC 1 cut(s) 766
TfiI GAWTC 4 cut(s) 200, 222, 343, 808
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 3 cut(s) 54, 759, 845
TseFI GTSAC 3 cut(s) 232, 754, 796
TseI GCWGC 3 cut(s) 84, 291, 706
Tsp45I GTSAC 3 cut(s) 232, 754, 796
TspDTI ATGAA 4 cut(s) 65, 800, 820, 828
TspGWI ACGGA 2 cut(s) 422, 671
TspRI CASTG 3 cut(s) 54, 759, 845
XapI RAATTY 5 cut(s) 464, 586, 622, 728, 815
XbaI TCTAGA 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 849
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 2 cut(s) 18, 287
Zsp2I ATGCAT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.