MD05G1115500.v1.1

PI-PLC X-box domain-containing protein DDB_G0293730-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005813
Physical Location & Seq
Forward (+)
23661766 .. 23662725
960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1115500.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGGTTCCTGCTTCCCTAAACAGCTCGAACGCCGGAAGGCGATCTCAACGGAGCGGAAAGCACTACGTGATCTCCACCAAAGCGTTGGTGAGGACTTCCCAGCATGTGCCTACAGGCCTCCGGACAGGAAAAATTGGATGGCGGGAATCAACCCGAAAAAAGTTCACGTAAACAAAATCGTATGGCCGGGAACTCATGACTCTGCAACCGACAAGATTGGAATTCCATGCATCACTAGGCCTTTTGGACAATGCCAAACAATGTCTATATACAAACAGCTTGTCACAGGCACCAGAGTGCTTGACATTCGGGTTCAAAAAGATCGCCGGGTGTGCCACGGAATTCTTATCACATACCACATTGATGTTGTGATCAAGGACATCAAAAAATTTCTGTCCGAAACAAAGTCTGAGATCATACTCCTTGAAATCAGGACGGAATATGGGCACCAAGACCCTCCTGATTTCGACAAGTATTTGGTGGATGAGCTCGGAGAGGTCCTTATCAACCAGGACGACAATGTGTTCAACAAGACTATTTCAGAGTTGTTGCCGAAGCGAATAATATGCGTGTGGAAGCCGAGGAATTCACCTTCCCCTGAGGCAGGGGCGCCTCTGTGGAATTCTGTCTACTTGAATGACGATTGGATTGACACGGATTTGCCATCAACGAAATTCGAGAACAATTTGAAGTGTTTGAGTGAACAACCTCCAATTTCATCAAGGAGATTTTTTTACAGGGTGGAGAACACAGTGACACCTCAGGCTGATAACCCTGTTGTGTGTGTTAGGCCTGTGACCGAACGGATTCACGAGTTTGCGAGGTTGTTTATAACGGAGTGCTTCTCGAGGGGTATTGCAGATAGGTTGCAAATCTTCTCTACTGATTTTATAGATGAGGATTTTGTTGATGCATGTGTTGCTGTTACATACTCAAGAATTGATCACGGGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PI-PLC-X

Protein Analysis

320

Amino Acids

36.67

Weight (kDa)

7.06

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 64 - 177 7.3e-10 Phosphatidylinositol-specific phospholipase C, X domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AccB1I GGYRCC 3 cut(s) 290, 447, 610
AccBSI CCGCTC 1 cut(s) 55
AccI GTMKAC 1 cut(s) 630
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 2 cut(s) 55, 143
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 6 cut(s) 222, 342, 389, 586, 622, 674
AcyI GRCGYC 1 cut(s) 611
AdeI CACNNNGTG 1 cut(s) 68
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 5 cut(s) 317, 428, 529, 637, 691
AjnI CCWGG 1 cut(s) 510
AleI CACNNNNGTG 1 cut(s) 296
AluBI AGCT 3 cut(s) 25, 280, 490
AluI AGCT 3 cut(s) 25, 280, 490
Alw21I GWGCWC 1 cut(s) 492
Ama87I CYCGRG 1 cut(s) 847
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 4 cut(s) 116, 185, 239, 791
ApoI RAATTY 6 cut(s) 222, 342, 389, 586, 622, 674
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
Asp700I GAANNNNTTC 1 cut(s) 807
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 1 cut(s) 499
AsuC2I CCSGG 2 cut(s) 189, 329
AsuHPI GGTGA 2 cut(s) 101, 582
AvaI CYCGRG 1 cut(s) 847
AvaII GGWCC 1 cut(s) 499
AxyI CCTNAGG 2 cut(s) 600, 762
BaeGI GKGCMC 1 cut(s) 450
BanI GGYRCC 3 cut(s) 290, 447, 610
BanII GRGCYC 1 cut(s) 492
BauI CACGAG 1 cut(s) 812
Bbv12I GWGCWC 1 cut(s) 492
BccI CCATC 2 cut(s) 133, 673
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 1 cut(s) 512
BclI TGATCA 2 cut(s) 372, 943
BcnI CCSGG 2 cut(s) 189, 329
BfaI CTAG 1 cut(s) 237
BfmI CTRYAG 1 cut(s) 112
BfoI RGCGCY 1 cut(s) 614
Bme1390I CCNGG 3 cut(s) 189, 329, 512
Bme18I GGWCC 1 cut(s) 499
BmeT110I CYCGRG 1 cut(s) 847
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 4 cut(s) 7, 292, 449, 612
BmrFI CCNGG 3 cut(s) 189, 329, 512
BmsI GCATC 2 cut(s) 240, 901
BplI GAGNNNNNCTC 2 cut(s) 830, 862
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 2 cut(s) 189, 329
BsaAI YACGTR 2 cut(s) 68, 169
BsaHI GRCGYC 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 337, 581
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse21I CCTNAGG 2 cut(s) 600, 762
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 2 cut(s) 337, 581
BseGI GGATG 2 cut(s) 144, 490
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMII CTCAG 3 cut(s) 402, 591, 776
BseSI GKGCMC 1 cut(s) 450
BseYI CCCAGC 1 cut(s) 100
BshFI GGCC 4 cut(s) 118, 187, 241, 793
BshNI GGYRCC 3 cut(s) 290, 447, 610
BsiHKAI GWGCWC 1 cut(s) 492
BsiHKCI CYCGRG 1 cut(s) 847
BsiSI CCGG 4 cut(s) 34, 122, 188, 328
BslI CCNNNNNNNGG 1 cut(s) 605
BsnI GGCC 4 cut(s) 118, 187, 241, 793
BsoBI CYCGRG 1 cut(s) 847
Bsp1286I GDGCHC 2 cut(s) 450, 492
Bsp13I TCCGGA 1 cut(s) 121
Bsp143I GATC 6 cut(s) 42, 70, 322, 372, 414, 943
BspACI CCGC 2 cut(s) 55, 143
BspANI GGCC 4 cut(s) 118, 187, 241, 793
BspCNI CTCAG 3 cut(s) 403, 592, 775
BspEI TCCGGA 1 cut(s) 121
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 4 cut(s) 7, 292, 449, 612
BspT107I GGYRCC 3 cut(s) 290, 447, 610
BsrBI CCGCTC 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 337, 581
BssMI GATC 6 cut(s) 42, 70, 322, 372, 414, 943
BssNI GRCGYC 1 cut(s) 611
BssSI CACGAG 1 cut(s) 812
Bst2BI CACGAG 1 cut(s) 812
Bst2UI CCWGG 1 cut(s) 512
Bst4CI ACNGT 1 cut(s) 754
BstACI GRCGYC 1 cut(s) 611
BstAPI GCANNNNNTGC 1 cut(s) 920
BstBAI YACGTR 2 cut(s) 68, 169
BstDEI CTNAG 3 cut(s) 411, 600, 762
BstDSI CCRYGG 1 cut(s) 337
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 144, 490
BstH2I RGCGCY 1 cut(s) 614
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 6 cut(s) 45, 73, 325, 375, 417, 946
BstMBI GATC 6 cut(s) 42, 70, 322, 372, 414, 943
BstMWI GCNNNNNNNGC 2 cut(s) 333, 920
BstNI CCWGG 1 cut(s) 512
BstNSI RCATGY 2 cut(s) 108, 918
BstSCI CCNGG 3 cut(s) 187, 327, 510
BstSFI CTRYAG 1 cut(s) 112
BstSLI GKGCMC 1 cut(s) 450
BstXI CCANNNNNNTGG 1 cut(s) 86
Bsu36I CCTNAGG 2 cut(s) 600, 762
BsuRI GGCC 4 cut(s) 118, 187, 241, 793
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 2 cut(s) 144, 490
BtsIMutI CAGTG 1 cut(s) 759
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 613
Cfr13I GGNCC 1 cut(s) 499
CviAII CATG 4 cut(s) 105, 197, 228, 915
DdeI CTNAG 3 cut(s) 411, 600, 762
DinI GGCGCC 1 cut(s) 612
DpnI GATC 6 cut(s) 44, 72, 324, 374, 416, 945
DpnII GATC 6 cut(s) 42, 70, 322, 372, 414, 943
DraIII CACNNNGTG 1 cut(s) 68
EaeI YGGCCR 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 490
Eco147I AGGCCT 3 cut(s) 118, 241, 793
Eco24I GRGCYC 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 499
Eco53kI GAGCTC 1 cut(s) 490
Eco81I CCTNAGG 2 cut(s) 600, 762
Eco88I CYCGRG 1 cut(s) 847
EcoICRI GAGCTC 1 cut(s) 490
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoO109I RGGNCCY 1 cut(s) 499
EcoRI GAATTC 4 cut(s) 222, 342, 586, 622
EcoRII CCWGG 1 cut(s) 510
EcoT22I ATGCAT 2 cut(s) 233, 916
EcoT38I GRGCYC 1 cut(s) 492
EgeI GGCGCC 1 cut(s) 612
EheI GGCGCC 1 cut(s) 612
FaeI CATG 4 cut(s) 108, 200, 231, 918
FatI CATG 4 cut(s) 104, 196, 227, 914
FauI CCCGC 1 cut(s) 136
FbaI TGATCA 2 cut(s) 372, 943
FblI GTMKAC 1 cut(s) 630
FokI GGATG 2 cut(s) 151, 497
FriOI GRGCYC 1 cut(s) 492
FspBI CTAG 1 cut(s) 237
GlaI GCGC 1 cut(s) 612
GsaI CCCAGC 1 cut(s) 104
HaeII RGCGCY 1 cut(s) 614
HaeIII GGCC 4 cut(s) 118, 187, 241, 793
HapII CCGG 4 cut(s) 34, 122, 188, 328
HhaI GCGC 1 cut(s) 613
Hin1I GRCGYC 1 cut(s) 611
Hin1II CATG 4 cut(s) 108, 200, 231, 918
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HinfI GANTC 3 cut(s) 147, 200, 808
HpaII CCGG 4 cut(s) 34, 122, 188, 328
HphI GGTGA 2 cut(s) 101, 582
Hpy166II GTNNAC 4 cut(s) 166, 172, 631, 704
Hpy188I TCNGA 4 cut(s) 400, 412, 494, 544
Hpy188III TCNNGA 8 cut(s) 122, 197, 433, 461, 679, 812, 847, 936
Hpy8I GTNNAC 4 cut(s) 166, 172, 631, 704
HpyAV CCTTC 2 cut(s) 31, 603
HpyCH4III ACNGT 1 cut(s) 754
HpyCH4IV ACGT 2 cut(s) 67, 168
HpyCH4V TGCA 5 cut(s) 206, 231, 860, 871, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 920
HpyF3I CTNAG 3 cut(s) 411, 600, 762
HpySE526I ACGT 2 cut(s) 67, 168
Hsp92I GRCGYC 1 cut(s) 611
Hsp92II CATG 4 cut(s) 108, 200, 231, 918
HspAI GCGC 1 cut(s) 611
KasI GGCGCC 1 cut(s) 610
Kpn2I TCCGGA 1 cut(s) 121
Ksp22I TGATCA 2 cut(s) 372, 943
Kzo9I GATC 6 cut(s) 42, 70, 322, 372, 414, 943
LmnI GCTCC 1 cut(s) 52
LweI GCATC 2 cut(s) 240, 901
MaeI CTAG 1 cut(s) 237
MaeII ACGT 2 cut(s) 67, 168
MaeIII GTNAC 4 cut(s) 283, 754, 796, 925
MalI GATC 6 cut(s) 44, 72, 324, 374, 416, 945
MbiI CCGCTC 1 cut(s) 55
MboI GATC 6 cut(s) 42, 70, 322, 372, 414, 943
MboII GAAGA 1 cut(s) 868
MhlI GDGCHC 2 cut(s) 450, 492
Mly113I GGCGCC 1 cut(s) 611
MlyI GAGTC 1 cut(s) 194
Mph1103I ATGCAT 2 cut(s) 233, 916
MroI TCCGGA 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 807
MslI CAYNNNNRTG 2 cut(s) 296, 363
MspI CCGG 4 cut(s) 34, 122, 188, 328
MspR9I CCNGG 3 cut(s) 189, 329, 512
MvaI CCWGG 1 cut(s) 512
MwoI GCNNNNNNNGC 2 cut(s) 333, 920
NarI GGCGCC 1 cut(s) 611
NciI CCSGG 2 cut(s) 189, 329
NdeII GATC 6 cut(s) 42, 70, 322, 372, 414, 943
NlaIII CATG 4 cut(s) 108, 200, 231, 918
NlaIV GGNNCC 4 cut(s) 7, 292, 449, 612
NmeAIII GCCGAG 1 cut(s) 606
NmuCI GTSAC 3 cut(s) 283, 754, 796
NsiI ATGCAT 2 cut(s) 233, 916
NspI RCATGY 2 cut(s) 108, 918
OliI CACNNNNGTG 1 cut(s) 296
PaeR7I CTCGAG 1 cut(s) 847
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 3 cut(s) 118, 241, 793
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 2 cut(s) 147, 808
PleI GAGTC 1 cut(s) 194
PluTI GGCGCC 1 cut(s) 614
PpsI GAGTC 1 cut(s) 194
Ppu21I YACGTR 2 cut(s) 68, 169
PpuMI RGGWCCY 1 cut(s) 499
PsiI TTATAA 1 cut(s) 833
Psp124BI GAGCTC 1 cut(s) 492
Psp5II RGGWCCY 1 cut(s) 499
Psp6I CCWGG 1 cut(s) 510
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 1 cut(s) 510
PspN4I GGNNCC 4 cut(s) 7, 292, 449, 612
PspPI GGNCC 1 cut(s) 499
PspPPI RGGWCCY 1 cut(s) 499
RseI CAYNNNNRTG 2 cut(s) 296, 363
SacI GAGCTC 1 cut(s) 492
Sau3AI GATC 6 cut(s) 42, 70, 322, 372, 414, 943
Sau96I GGNCC 1 cut(s) 499
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 3 cut(s) 189, 329, 512
SduI GDGCHC 2 cut(s) 450, 492
SfaNI GCATC 2 cut(s) 240, 901
SfcI CTRYAG 1 cut(s) 112
SfoI GGCGCC 1 cut(s) 612
Sfr274I CTCGAG 1 cut(s) 847
SinI GGWCC 1 cut(s) 499
SlaI CTCGAG 1 cut(s) 847
SmiMI CAYNNNNRTG 2 cut(s) 296, 363
SmlI CTYRAG 2 cut(s) 847, 934
SmoI CTYRAG 2 cut(s) 847, 934
SseBI AGGCCT 3 cut(s) 118, 241, 793
SsiI CCGC 2 cut(s) 55, 143
SspDI GGCGCC 1 cut(s) 610
SspMI CTAG 1 cut(s) 237
SstI GAGCTC 1 cut(s) 492
StuI AGGCCT 3 cut(s) 118, 241, 793
StyD4I CCNGG 3 cut(s) 187, 327, 510
TaaI ACNGT 1 cut(s) 754
TaiI ACGT 2 cut(s) 70, 171
TaqI TCGA 4 cut(s) 27, 468, 678, 848
TaqII GACCGA 1 cut(s) 815
TfiI GAWTC 2 cut(s) 147, 808
TscAI CASTG 1 cut(s) 759
TseFI GTSAC 3 cut(s) 283, 754, 796
Tsp45I GTSAC 3 cut(s) 283, 754, 796
TspDTI ATGAA 1 cut(s) 708
TspGWI ACGGA 6 cut(s) 65, 354, 452, 671, 820, 851
TspRI CASTG 1 cut(s) 759
VpaK11BI GGWCC 1 cut(s) 499
XagI CCTNNNNNAGG 1 cut(s) 603
XapI RAATTY 6 cut(s) 222, 342, 389, 586, 622, 674
XceI RCATGY 2 cut(s) 108, 918
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XhoI CTCGAG 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 630
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 1 cut(s) 237
Zsp2I ATGCAT 2 cut(s) 233, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.