FvH4_4g25590

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014722
Physical Location & Seq
Forward (+)
27305282 .. 27306577
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25590.t1

Sequence Viewer

Length: 1020 bp
ATGGCATCCTACCAGATGGCCAATGACATCAACTCTCTTTCCCTTTCCCCAAATTTCATTCTCCCAGAGAATCACAGGCCACACCTCACTCAGGTTTCAACTTTGGATTCAATACCTATCATCGACTTGAATGACACTGACACCATCAATTTGGTTCAGAAGATATCACAAGCTTCTGAGGTCTATGGCTTTTTCCAGATCATCAACCATGGAGTACCTGAGGAGTTATGCAATAGAATGATGACTGCTTATACCCAATTCTTCAAACTTCCTGCTGAAGAAAAGGCACAGTACTTCACTACAGATCACACCAAACAGGTTAAGCTTTTCAACTATTTCCTCAAGATTCAAGGTCAAGAAAAGGTCACCATGTGGAGCGAAACCTTTTCCCATTTTTGGCATCCCACCAATCTGTTACCTGAAAATCCTCCCCAATACCGTGAGGTTTTTATTGAATATGCAAAAGAGATTGGTACATTGATGAATAGGCTATTGGAATTGCTCTCCCACGGACTTGGCCTGGAGAAAGATTGCTTGCATAAGAAGCTGGGTGAGAATCCCACTCTAAAAGCTCAAGCAAACTACTACCCACCATGTCCTGACCCTGAGCTCACACTCGGACTGGCTGTTCATACTGATCTCAATGCACTCACTGTGCTTAGGCAAACAGAGGGAGTGAAGGGCCTTCAAGTCATCAAAGATGGGAAATGGGTTGGTGTTGATCCCCTCTCCAATGCATTTGTTATCAACCTTGGTGATCAAATTCAGGTTCTAAGCAATGGAAGGTATAAGAGTGTGCACCATAGGGCAGTAACCAATAAAACTCGGCCAAGGTTATCAATAGCAATGTTCTATGGACCCAACAAGGACACACAGATTGGTCCAATTGAGGAATTGATAGATGAGGAACACCCTCCATTGTATAGAAGCTATCACTATGCAGAGTTTCTTGAAGAATTCTTCAGGCAAGAAGGTACAAGAAGGCTGGTGAAGGAAGTCTTTGAGTTGCACCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

340

Amino Acids

39.05

Weight (kDa)

5.92

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 38 - 136 1.7e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 193 - 287 3.7e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014722)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25590
malus_domestica MD16G1101600.v1.1 MD16G1101900.v1.1
prunus_persica Prupe.8G011800_v2.0.a1
pyrus_communis pycom16g08630
rosa_chinensis RchiOBHm_Chr5g0016311
rosa_laevigata RLG00000006792
rosa_multiflora Rmu_co8454895.1_g000001 Rmu_sc0001762.1_g000036
rosa_roxburghii Rroxscaffold_5G00374460
rosa_samantha Rh1AG277500 Rh4AG318700 Rh4BG326800 Rh4CG342200 Rh4DG322000 Rh5CG136300
rosa_wichuraiana Rw4G022550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 716
AcoI YGGCCR 2 cut(s) 18, 827
AcsI RAATTY 3 cut(s) 52, 762, 956
AcuI CTGAAG 2 cut(s) 297, 946
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 4 cut(s) 216, 293, 475, 976
AfiI CCNNNNNNNGG 2 cut(s) 91, 396
AgsI TTSAA 9 cut(s) 99, 111, 130, 265, 331, 350, 455, 689, 953
AjnI CCWGG 1 cut(s) 519
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AloI GAACNNNNNNTCC 2 cut(s) 612, 644
AluBI AGCT 6 cut(s) 173, 325, 547, 572, 610, 930
AluI AGCT 6 cut(s) 173, 325, 547, 572, 610, 930
Alw21I GWGCWC 2 cut(s) 612, 801
Alw44I GTGCAC 1 cut(s) 797
AlwI GGATC 1 cut(s) 716
AoxI GGCC 5 cut(s) 18, 77, 517, 682, 827
ApaLI GTGCAC 1 cut(s) 797
ApoI RAATTY 3 cut(s) 52, 762, 956
AspS9I GGNCC 3 cut(s) 682, 857, 881
AsuHPI GGTGA 4 cut(s) 358, 563, 767, 1000
AvaII GGWCC 2 cut(s) 857, 881
AxyI CCTNAGG 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 801
BalI TGGCCA 1 cut(s) 20
BanII GRGCYC 1 cut(s) 612
Bbv12I GWGCWC 2 cut(s) 612, 801
BccI CCATC 3 cut(s) 10, 152, 695
BciT130I CCWGG 1 cut(s) 521
BclI TGATCA 1 cut(s) 757
BfmI CTRYAG 1 cut(s) 300
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 1 cut(s) 521
Bme18I GGWCC 2 cut(s) 857, 881
BmgT120I GGNCC 3 cut(s) 682, 857, 881
BmiI GGNNCC 1 cut(s) 859
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 2 cut(s) 14, 409
BpmI CTGGAG 1 cut(s) 542
Bpu10I CCTNAGC 2 cut(s) 606, 659
BpuEI CTTGAG 2 cut(s) 326, 558
BsaJI CCNNGG 4 cut(s) 208, 508, 751, 830
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 396
Bse1I ACTGG 1 cut(s) 627
Bse21I CCTNAGG 1 cut(s) 219
Bse3DI GCAATG 2 cut(s) 784, 852
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 4 cut(s) 208, 508, 751, 830
BseGI GGATG 2 cut(s) 5, 400
BseLI CCNNNNNNNGG 2 cut(s) 91, 396
BseMI GCAATG 2 cut(s) 784, 852
BseMII CTCAG 4 cut(s) 104, 168, 210, 597
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 801
BseYI CCCAGC 1 cut(s) 547
BshFI GGCC 5 cut(s) 20, 79, 519, 684, 829
BsiHKAI GWGCWC 2 cut(s) 612, 801
BslI CCNNNNNNNGG 2 cut(s) 91, 396
BsnI GGCC 5 cut(s) 20, 79, 519, 684, 829
Bsp1286I GDGCHC 2 cut(s) 612, 801
Bsp143I GATC 5 cut(s) 198, 304, 637, 721, 757
Bsp19I CCATGG 1 cut(s) 208
BspANI GGCC 5 cut(s) 20, 79, 519, 684, 829
BspCNI CTCAG 4 cut(s) 103, 169, 211, 598
BspLI GGNNCC 1 cut(s) 859
BspPI GGATC 1 cut(s) 716
BsrDI GCAATG 2 cut(s) 784, 852
BsrI ACTGG 1 cut(s) 627
BssECI CCNNGG 4 cut(s) 208, 508, 751, 830
BssMI GATC 5 cut(s) 198, 304, 637, 721, 757
BssT1I CCWWGG 3 cut(s) 208, 751, 830
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 3 cut(s) 291, 440, 655
BstC8I GCNNGC 1 cut(s) 536
BstDEI CTNAG 6 cut(s) 90, 177, 219, 606, 659, 773
BstDSI CCRYGG 2 cut(s) 208, 508
BstEII GGTNACC 1 cut(s) 364
BstENI CCTNNNNNAGG 1 cut(s) 89
BstF5I GGATG 2 cut(s) 5, 400
BstKTI GATC 5 cut(s) 201, 307, 640, 724, 760
BstMBI GATC 5 cut(s) 198, 304, 637, 721, 757
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 1 cut(s) 521
BstPI GGTNACC 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 801
BstXI CCANNNNNNTGG 2 cut(s) 151, 515
Bsu36I CCTNAGG 1 cut(s) 219
BsuRI GGCC 5 cut(s) 20, 79, 519, 684, 829
BtgI CCRYGG 2 cut(s) 208, 508
BtsCI GGATG 2 cut(s) 5, 400
BtsIMutI CAGTG 2 cut(s) 135, 651
Cac8I GCNNGC 1 cut(s) 536
Cfr13I GGNCC 3 cut(s) 682, 857, 881
Csp6I GTAC 4 cut(s) 215, 292, 474, 975
CviAII CATG 3 cut(s) 209, 370, 594
CviQI GTAC 4 cut(s) 215, 292, 474, 975
DdeI CTNAG 6 cut(s) 90, 177, 219, 606, 659, 773
DpnI GATC 5 cut(s) 200, 306, 639, 723, 759
DpnII GATC 5 cut(s) 198, 304, 637, 721, 757
DraIII CACNNNGTG 1 cut(s) 372
EaeI YGGCCR 2 cut(s) 18, 827
Ecl136II GAGCTC 1 cut(s) 610
Eco130I CCWWGG 3 cut(s) 208, 751, 830
Eco24I GRGCYC 1 cut(s) 612
Eco32I GATATC 1 cut(s) 165
Eco47I GGWCC 2 cut(s) 857, 881
Eco53kI GAGCTC 1 cut(s) 610
Eco57I CTGAAG 2 cut(s) 297, 946
Eco81I CCTNAGG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 364
EcoICRI GAGCTC 1 cut(s) 610
EcoNI CCTNNNNNAGG 1 cut(s) 89
EcoO109I RGGNCCY 1 cut(s) 682
EcoO65I GGTNACC 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 956
EcoRII CCWGG 1 cut(s) 519
EcoRV GATATC 1 cut(s) 165
EcoT14I CCWWGG 3 cut(s) 208, 751, 830
EcoT22I ATGCAT 1 cut(s) 739
EcoT38I GRGCYC 1 cut(s) 612
ErhI CCWWGG 3 cut(s) 208, 751, 830
FaeI CATG 3 cut(s) 212, 373, 597
FalI AAGNNNNNCTT 2 cut(s) 983, 1015
FatI CATG 3 cut(s) 208, 369, 593
FbaI TGATCA 1 cut(s) 757
FokI GGATG 1 cut(s) 387
FriOI GRGCYC 1 cut(s) 612
GsaI CCCAGC 1 cut(s) 551
GsuI CTGGAG 1 cut(s) 542
HaeIII GGCC 5 cut(s) 20, 79, 519, 684, 829
Hin1II CATG 3 cut(s) 212, 373, 597
HindIII AAGCTT 2 cut(s) 171, 323
HinfI GANTC 4 cut(s) 70, 107, 346, 556
HphI GGTGA 4 cut(s) 358, 563, 767, 1000
Hpy166II GTNNAC 1 cut(s) 799
Hpy188I TCNGA 3 cut(s) 159, 178, 620
Hpy188III TCNNGA 5 cut(s) 196, 343, 356, 599, 950
Hpy8I GTNNAC 1 cut(s) 799
HpyAV CCTTC 6 cut(s) 673, 695, 777, 965, 975, 985
HpyCH4III ACNGT 3 cut(s) 291, 440, 655
HpyCH4V TGCA 8 cut(s) 231, 461, 538, 647, 737, 799, 941, 1009
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 6 cut(s) 90, 177, 219, 606, 659, 773
Hsp92II CATG 3 cut(s) 212, 373, 597
Ksp22I TGATCA 1 cut(s) 757
Kzo9I GATC 5 cut(s) 198, 304, 637, 721, 757
LmnI GCTCC 1 cut(s) 375
LweI GCATC 2 cut(s) 14, 409
MaeIII GTNAC 3 cut(s) 364, 414, 811
MalI GATC 5 cut(s) 200, 306, 639, 723, 759
MboI GATC 5 cut(s) 198, 304, 637, 721, 757
MboII GAAGA 5 cut(s) 172, 253, 290, 952, 965
MfeI CAATTG 1 cut(s) 885
MhlI GDGCHC 2 cut(s) 612, 801
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 9 cut(s) 52, 148, 257, 497, 762, 885, 893, 956, 1015
MluNI TGGCCA 1 cut(s) 20
Mox20I TGGCCA 1 cut(s) 20
Mph1103I ATGCAT 1 cut(s) 739
MscI TGGCCA 1 cut(s) 20
MseI TTAA 2 cut(s) 321, 1018
Msp20I TGGCCA 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 521
MunI CAATTG 1 cut(s) 885
MvaI CCWGG 1 cut(s) 521
MwoI GCNNNNNNNGC 1 cut(s) 544
NcoI CCATGG 1 cut(s) 208
NdeII GATC 5 cut(s) 198, 304, 637, 721, 757
NlaIII CATG 3 cut(s) 212, 373, 597
NlaIV GGNNCC 1 cut(s) 859
NmeAIII GCCGAG 1 cut(s) 805
NmuCI GTSAC 1 cut(s) 364
NsiI ATGCAT 1 cut(s) 739
PfeI GAWTC 4 cut(s) 70, 107, 346, 556
Psp124BI GAGCTC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 519
PspEI GGTNACC 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 547
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 859
PspPI GGNCC 3 cut(s) 682, 857, 881
RsaI GTAC 4 cut(s) 216, 293, 475, 976
RsaNI GTAC 4 cut(s) 215, 292, 474, 975
SacI GAGCTC 1 cut(s) 612
SaqAI TTAA 2 cut(s) 321, 1018
Sau3AI GATC 5 cut(s) 198, 304, 637, 721, 757
Sau96I GGNCC 3 cut(s) 682, 857, 881
ScaI AGTACT 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 2 cut(s) 612, 801
SfaNI GCATC 2 cut(s) 14, 409
SfcI CTRYAG 1 cut(s) 300
SinI GGWCC 2 cut(s) 857, 881
SmlI CTYRAG 2 cut(s) 341, 573
SmoI CTYRAG 2 cut(s) 341, 573
Sse9I AATT 9 cut(s) 52, 148, 257, 497, 762, 885, 893, 956, 1015
SstI GAGCTC 1 cut(s) 612
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 3 cut(s) 208, 751, 830
TaaI ACNGT 3 cut(s) 291, 440, 655
TaqI TCGA 1 cut(s) 123
TasI AATT 9 cut(s) 52, 148, 257, 497, 762, 885, 893, 956, 1015
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 4 cut(s) 70, 107, 346, 556
Tru1I TTAA 2 cut(s) 321, 1018
Tru9I TTAA 2 cut(s) 321, 1018
TscAI CASTG 2 cut(s) 142, 658
TseFI GTSAC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 3 cut(s) 46, 497, 620
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 2 cut(s) 142, 658
VneI GTGCAC 1 cut(s) 797
VpaK11BI GGWCC 2 cut(s) 857, 881
XagI CCTNNNNNAGG 1 cut(s) 89
XapI RAATTY 3 cut(s) 52, 762, 956
ZrmI AGTACT 1 cut(s) 293
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.