MD16G1101600.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014722
Physical Location & Seq
Reverse (-)
7093010 .. 7094220
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1101600.v1.1.491

Sequence Viewer

Length: 720 bp
ATGATGACTGCCATGGCCCAGTTCTTCAAATTGCCTCCTGAAGAAAGGGCAAAATACTTCACCACTGATCACTCCAAGCAAGTAAAGCTTTTCAATTTCTACCTCAAAGTTGAAGGGCAAGAGCAAAAGGTCACTATGTGGAGTGAAACCTTCTCACATCTTTGGCATCCTCTTAATTCCTTGCCTGAAAATCCTCCCCAATACGGATTGATGAAAACGCTCTTGGGATTGCTATCCCAGGGGCTTGGACTAGAGAAGGACTGCTTACAAAAGAAGCTGGATGATAATCCTACTCTGAAAGCACAGGGAAACTACCATCCACCATGTCCAGACCCTGAGGTCACACTTGGACTAGCTATCCATACTGATCTCAATGCACTCACAGTACTCAAGCAGACAGAGGGAGTGACTGGCCTTCAAGTGATCAAAGATGGGAAATGGGTTGCTGTCGATTCTCTCCCCCAATGCCTTTGTATAAGAGTGTGCACCACAGCCCACAGGGCAGTGACCAACAAAATTCAGCCACGTCTGTCATTGGCAATGTTTTATGGACCCAACAGGGACACTGTGATTGGCCCAATTAAGGATTTGATTGATGAGCAACACTCTACATTGTATCGGAACTACAAGTATGCAGAGTTTTTGAAGAGTTCTACAGGCAAGAAGGAGCAAGAAGGAGCAAGAAGGTTTGTCAAGGAAGTTTTTGAGTTGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

240

Amino Acids

27.2

Weight (kDa)

8.49

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 104 - 185 2.2e-14 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014722)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25590
malus_domestica MD16G1101600.v1.1 MD16G1101900.v1.1
prunus_persica Prupe.8G011800_v2.0.a1
pyrus_communis pycom16g08630
rosa_chinensis RchiOBHm_Chr5g0016311
rosa_laevigata RLG00000006792
rosa_multiflora Rmu_co8454895.1_g000001 Rmu_sc0001762.1_g000036
rosa_roxburghii Rroxscaffold_5G00374460
rosa_samantha Rh1AG277500 Rh4AG318700 Rh4BG326800 Rh4CG342200 Rh4DG322000 Rh5CG136300
rosa_wichuraiana Rw4G022550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AcsI RAATTY 1 cut(s) 516
AcuI CTGAAG 1 cut(s) 60
AdeI CACNNNGTG 1 cut(s) 138
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 2 cut(s) 203, 583
AgsI TTSAA 5 cut(s) 28, 94, 113, 419, 646
AjiI CACGTC 1 cut(s) 527
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 3 cut(s) 88, 277, 356
AluI AGCT 3 cut(s) 88, 277, 356
Alw21I GWGCWC 1 cut(s) 488
Alw44I GTGCAC 1 cut(s) 484
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 3 cut(s) 15, 412, 574
ApaLI GTGCAC 1 cut(s) 484
ApoI RAATTY 1 cut(s) 516
AspS9I GGNCC 3 cut(s) 16, 551, 575
AsuHPI GGTGA 1 cut(s) 52
AvaII GGWCC 1 cut(s) 551
AxyI CCTNAGG 1 cut(s) 336
BaeGI GKGCMC 1 cut(s) 488
Bbv12I GWGCWC 1 cut(s) 488
BccI CCATC 2 cut(s) 324, 425
BciT130I CCWGG 1 cut(s) 239
BclI TGATCA 2 cut(s) 67, 423
BfaI CTAG 2 cut(s) 251, 353
BfmI CTRYAG 1 cut(s) 654
BglI GCCNNNNNGGC 1 cut(s) 500
BmcAI AGTACT 1 cut(s) 387
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 551
BmgBI CACGTC 1 cut(s) 527
BmgT120I GGNCC 3 cut(s) 16, 551, 575
BmiI GGNNCC 1 cut(s) 553
BmrFI CCNGG 1 cut(s) 239
BmrI ACTGGG 1 cut(s) 13
BmsI GCATC 1 cut(s) 175
BmuI ACTGGG 1 cut(s) 13
BplI GAGNNNNNCTC 2 cut(s) 590, 622
BpuEI CTTGAG 1 cut(s) 374
BsaBI GATNNNNATC 2 cut(s) 232, 285
BsaJI CCNNGG 3 cut(s) 12, 237, 238
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 583
Bse1I ACTGG 2 cut(s) 19, 415
Bse21I CCTNAGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 546
Bse8I GATNNNNATC 2 cut(s) 232, 285
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 3 cut(s) 12, 237, 238
BseGI GGATG 3 cut(s) 166, 286, 316
BseJI GATNNNNATC 2 cut(s) 232, 285
BseLI CCNNNNNNNGG 2 cut(s) 203, 583
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 2 cut(s) 19, 415
BseSI GKGCMC 1 cut(s) 488
BshFI GGCC 3 cut(s) 17, 414, 576
BsiHKAI GWGCWC 1 cut(s) 488
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 2 cut(s) 203, 583
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 3 cut(s) 17, 414, 576
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 3 cut(s) 67, 367, 423
Bsp19I CCATGG 1 cut(s) 12
BspANI GGCC 3 cut(s) 17, 414, 576
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 553
BsrDI GCAATG 1 cut(s) 546
BsrI ACTGG 2 cut(s) 19, 415
BssECI CCNNGG 3 cut(s) 12, 237, 238
BssMI GATC 3 cut(s) 67, 367, 423
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 2 cut(s) 385, 568
Bst6I CTCTTC 1 cut(s) 641
BstDEI CTNAG 1 cut(s) 336
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 166, 286, 316
BstKTI GATC 3 cut(s) 70, 370, 426
BstMBI GATC 3 cut(s) 67, 367, 423
BstMWI GCNNNNNNNGC 2 cut(s) 85, 500
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 1 cut(s) 488
BstXI CCANNNNNNTGG 1 cut(s) 245
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 3 cut(s) 17, 414, 576
BtgI CCRYGG 1 cut(s) 12
BtrI CACGTC 1 cut(s) 527
BtsCI GGATG 3 cut(s) 166, 286, 316
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 3 cut(s) 63, 510, 564
CaiI CAGNNNCTG 1 cut(s) 335
Cfr13I GGNCC 3 cut(s) 16, 551, 575
Csp6I GTAC 1 cut(s) 386
CviAII CATG 2 cut(s) 13, 324
CviJI RGCY 9 cut(s) 17, 88, 244, 277, 356, 414, 494, 523, 576
CviKI_1 RGCY 9 cut(s) 17, 88, 244, 277, 356, 414, 494, 523, 576
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 1 cut(s) 336
DpnI GATC 3 cut(s) 69, 369, 425
DpnII GATC 3 cut(s) 67, 367, 423
DraIII CACNNNGTG 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
Eco130I CCWWGG 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 551
Eco57I CTGAAG 1 cut(s) 60
Eco81I CCTNAGG 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 2 cut(s) 16, 327
FaiI YATR 7 cut(s) 14, 137, 325, 363, 476, 549, 633
FalI AAGNNNNNCTT 4 cut(s) 72, 104, 248, 280
FaqI GGGAC 1 cut(s) 575
FatI CATG 2 cut(s) 12, 323
FbaI TGATCA 2 cut(s) 67, 423
FokI GGATG 3 cut(s) 153, 293, 303
FspBI CTAG 2 cut(s) 251, 353
HaeIII GGCC 3 cut(s) 17, 414, 576
Hin1II CATG 2 cut(s) 16, 327
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 1 cut(s) 452
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 486
Hpy188I TCNGA 2 cut(s) 297, 621
Hpy188III TCNNGA 2 cut(s) 38, 329
Hpy8I GTNNAC 1 cut(s) 486
HpyAV CCTTC 7 cut(s) 107, 160, 250, 425, 658, 668, 678
HpyCH4III ACNGT 2 cut(s) 385, 568
HpyCH4IV ACGT 1 cut(s) 526
HpyCH4V TGCA 4 cut(s) 377, 486, 635, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 500
HpyF3I CTNAG 1 cut(s) 336
HpySE526I ACGT 1 cut(s) 526
Hsp92II CATG 2 cut(s) 16, 327
Ksp22I TGATCA 2 cut(s) 67, 423
Kzo9I GATC 3 cut(s) 67, 367, 423
LmnI GCTCC 2 cut(s) 667, 677
LweI GCATC 1 cut(s) 175
MaeI CTAG 2 cut(s) 251, 353
MaeII ACGT 1 cut(s) 526
MaeIII GTNAC 4 cut(s) 130, 340, 406, 505
MalI GATC 3 cut(s) 69, 369, 425
MboI GATC 3 cut(s) 67, 367, 423
MboII GAAGA 3 cut(s) 16, 53, 658
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 6 cut(s) 29, 94, 175, 516, 579, 715
MnlI CCTC 6 cut(s) 45, 113, 180, 204, 331, 394
MseI TTAA 2 cut(s) 174, 582
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 85, 500
NcoI CCATGG 1 cut(s) 12
NdeII GATC 3 cut(s) 67, 367, 423
NlaIII CATG 2 cut(s) 16, 327
NlaIV GGNNCC 1 cut(s) 553
NmuCI GTSAC 4 cut(s) 130, 340, 406, 505
PasI CCCWGGG 1 cut(s) 238
PfeI GAWTC 1 cut(s) 452
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 3 cut(s) 16, 551, 575
PstNI CAGNNNCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SaqAI TTAA 2 cut(s) 174, 582
Sau3AI GATC 3 cut(s) 67, 367, 423
Sau96I GGNCC 3 cut(s) 16, 551, 575
ScaI AGTACT 1 cut(s) 387
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 1 cut(s) 488
SetI ASST 9 cut(s) 90, 105, 132, 152, 279, 342, 358, 529, 689
SfaNI GCATC 1 cut(s) 175
SfcI CTRYAG 1 cut(s) 654
SinI GGWCC 1 cut(s) 551
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 6 cut(s) 29, 94, 175, 516, 579, 715
SspMI CTAG 2 cut(s) 251, 353
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 2 cut(s) 385, 568
TaiI ACGT 1 cut(s) 529
TaqI TCGA 1 cut(s) 450
TasI AATT 6 cut(s) 29, 94, 175, 516, 579, 715
TatI WGTACW 1 cut(s) 385
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 2 cut(s) 174, 582
Tru9I TTAA 2 cut(s) 174, 582
TscAI CASTG 3 cut(s) 70, 510, 571
TseFI GTSAC 4 cut(s) 130, 340, 406, 505
Tsp45I GTSAC 4 cut(s) 130, 340, 406, 505
TspDTI ATGAA 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 3 cut(s) 70, 510, 571
VneI GTGCAC 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 551
XapI RAATTY 1 cut(s) 516
XspI CTAG 2 cut(s) 251, 353
ZrmI AGTACT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.