Rh4CG342200

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014722
Physical Location & Seq
Forward (+)
60138938 .. 60139516
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG342200.1

Sequence Viewer

Length: 495 bp
ATGGCATCCTTCCAAATGGCCAATGACATCAACCCTCTTTCCCTTTCCCCAAATTTCATTCTCCCAGAGAGTCACAAGCCGCACCTCACTCAGATTTCAACTTTGGATTCAATTCCCATCATTGACTTGAATGACTATGACACTGACAACTTGGTTCAGAAGATATCACAAACTTCTGAGGTCTATGGCTTTTTCCAGATCATCAACCATGGAGTACCTGAGGAAATATGCGATAGAATGATGACTGCCTATACACAATTCTTCAAACTTTCTGCTGAAGAAAAGGCACAGTACTTCACTACAGATCACACCAAGCAGGTCAAGCTTTTCAACTACTTCCTCAAGATTCAAGGTCAAGAACAGGTCACCATGTGGAGTGAAACCTTCCCCCATCTTTGGCATCGCACCAATCTGTTACCTGAAAATCCTCCCCAATACCGTGAGGTTTTCATTGAATATGCAAAGGAGATTGGTACATTGATGAATAGGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

164

Amino Acids

19.24

Weight (kDa)

5.12

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 38 - 135 8e-20 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014722)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25590
malus_domestica MD16G1101600.v1.1 MD16G1101900.v1.1
prunus_persica Prupe.8G011800_v2.0.a1
pyrus_communis pycom16g08630
rosa_chinensis RchiOBHm_Chr5g0016311
rosa_laevigata RLG00000006792
rosa_multiflora Rmu_co8454895.1_g000001 Rmu_sc0001762.1_g000036
rosa_roxburghii Rroxscaffold_5G00374460
rosa_samantha Rh1AG277500 Rh4AG318700 Rh4BG326800 Rh4CG342200 Rh4DG322000 Rh5CG136300
rosa_wichuraiana Rw4G022550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 307
AciI CCGC 1 cut(s) 80
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 297
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 3 cut(s) 216, 293, 475
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 7 cut(s) 99, 111, 130, 265, 331, 350, 455
AluBI AGCT 1 cut(s) 325
AluI AGCT 1 cut(s) 325
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 219
BalI TGGCCA 1 cut(s) 20
BccI CCATC 2 cut(s) 125, 399
BfaI CTAG 1 cut(s) 493
BfmI CTRYAG 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 307
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
BmcAI AGTACT 1 cut(s) 293
BmsI GCATC 2 cut(s) 14, 409
BpuEI CTTGAG 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse21I CCTNAGG 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 1 cut(s) 5
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMII CTCAG 3 cut(s) 104, 168, 210
BshFI GGCC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 396
BsnI GGCC 1 cut(s) 20
Bsp143I GATC 2 cut(s) 198, 304
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 1 cut(s) 80
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 3 cut(s) 103, 169, 211
BspMI ACCTGC 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 2 cut(s) 198, 304
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 291, 440
BstDEI CTNAG 3 cut(s) 90, 177, 219
BstDSI CCRYGG 1 cut(s) 208
BstEII GGTNACC 1 cut(s) 364
BstF5I GGATG 1 cut(s) 5
BstKTI GATC 2 cut(s) 201, 307
BstMBI GATC 2 cut(s) 198, 304
BstMWI GCNNNNNNNGC 1 cut(s) 322
BstPI GGTNACC 1 cut(s) 364
BstSFI CTRYAG 1 cut(s) 300
Bsu36I CCTNAGG 1 cut(s) 219
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 208
BtgZI GCGATG 1 cut(s) 386
BtsCI GGATG 1 cut(s) 5
BtsIMutI CAGTG 1 cut(s) 141
BveI ACCTGC 1 cut(s) 307
Csp6I GTAC 3 cut(s) 215, 292, 474
CviAII CATG 2 cut(s) 209, 370
CviJI RGCY 5 cut(s) 20, 79, 189, 325, 490
CviKI_1 RGCY 5 cut(s) 20, 79, 189, 325, 490
CviQI GTAC 3 cut(s) 215, 292, 474
DdeI CTNAG 3 cut(s) 90, 177, 219
DpnI GATC 2 cut(s) 200, 306
DpnII GATC 2 cut(s) 198, 304
DraIII CACNNNGTG 1 cut(s) 372
EaeI YGGCCR 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 208
Eco32I GATATC 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 297
Eco81I CCTNAGG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 364
EcoRV GATATC 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 2 cut(s) 212, 373
FaiI YATR 7 cut(s) 138, 186, 210, 229, 252, 371, 459
FatI CATG 2 cut(s) 208, 369
Fnu4HI GCNGC 1 cut(s) 80
Fsp4HI GCNGC 1 cut(s) 80
FspBI CTAG 1 cut(s) 493
GluI GCNGC 1 cut(s) 80
HaeIII GGCC 1 cut(s) 20
Hin1II CATG 2 cut(s) 212, 373
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 3 cut(s) 70, 107, 346
HphI GGTGA 1 cut(s) 358
Hpy188I TCNGA 3 cut(s) 93, 159, 178
Hpy188III TCNNGA 3 cut(s) 196, 343, 356
HpyAV CCTTC 2 cut(s) 19, 394
HpyCH4III ACNGT 2 cut(s) 291, 440
HpyCH4V TGCA 1 cut(s) 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 322
HpyF3I CTNAG 3 cut(s) 90, 177, 219
Hsp92II CATG 2 cut(s) 212, 373
Kzo9I GATC 2 cut(s) 198, 304
LpnPI CCDG 6 cut(s) 78, 209, 231, 302, 347, 432
LweI GCATC 2 cut(s) 14, 409
MaeI CTAG 1 cut(s) 493
MaeIII GTNAC 3 cut(s) 71, 364, 414
MalI GATC 2 cut(s) 200, 306
MboI GATC 2 cut(s) 198, 304
MboII GAAGA 3 cut(s) 172, 253, 290
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 3 cut(s) 52, 111, 257
MluNI TGGCCA 1 cut(s) 20
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 7 cut(s) 45, 95, 172, 214, 350, 436, 438
Mox20I TGGCCA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 20
Msp20I TGGCCA 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 322
NcoI CCATGG 1 cut(s) 208
NdeII GATC 2 cut(s) 198, 304
NlaIII CATG 2 cut(s) 212, 373
NmuCI GTSAC 2 cut(s) 71, 364
PfeI GAWTC 2 cut(s) 107, 346
PkrI GCNGC 1 cut(s) 81
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PspEI GGTNACC 1 cut(s) 364
RsaI GTAC 3 cut(s) 216, 293, 475
RsaNI GTAC 3 cut(s) 215, 292, 474
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 2 cut(s) 198, 304
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 1 cut(s) 79
SfaNI GCATC 2 cut(s) 14, 409
SfcI CTRYAG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 3 cut(s) 52, 111, 257
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 493
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 2 cut(s) 291, 440
TasI AATT 3 cut(s) 52, 111, 257
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 2 cut(s) 107, 346
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 2 cut(s) 71, 364
Tsp45I GTSAC 2 cut(s) 71, 364
TspDTI ATGAA 2 cut(s) 46, 439
TspRI CASTG 1 cut(s) 148
XapI RAATTY 1 cut(s) 52
XspI CTAG 1 cut(s) 493
ZrmI AGTACT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.