Rh4BG326800

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014722
Physical Location & Seq
Forward (+)
49237405 .. 49254539
17135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG326800.1

Sequence Viewer

Length: 573 bp
ATGGCATCCTTCCAAATGGCCAATGACATCAACCCTCTTTCCCTTTCCCCAAATTTCATTCTCCCAGAGAGTCACAAGCCGCACCTCACTCAGATTTCAACTTTGGATTCAATTCCCATCATTGACTTGAATGACTATGACACTGACAACTTGGTTCAGAAGATATCACAAACTTCTGAGGTCTATGGCTTTTTCCAGATCATCAACCATGGAGTACCTGAGGAAATATGCGATAGAATGATGACTGCCTATACACAATTCTTCAAACTTTCTGCTGAAGAAAAGGCACAGTACTTCACTACAGATCACACCAAGCAGGTCAAGCTTTTCAACTACTTCCTCAAGATTCAAGGTCAAGAACAGGTCACCATGTGGAGTGAAACCTTCCCCCATCTTTGGCATCGCACCAATCTGTTACCTGAAAATCCTCCCCAATACCGACCTCTGAAGCGCTTGCACGTAGGATTTTCAGACTTCTGTTACGCAAATATGTGGTTAGACCAATTTGTGAAATTTTTTGTTACAGTCGTCATGCATGACTACAATTTGGGGCTTGTAAAGCACGTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

190

Amino Acids

22.34

Weight (kDa)

5.79

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 38 - 140 6.8e-20 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014722)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25590
malus_domestica MD16G1101600.v1.1 MD16G1101900.v1.1
prunus_persica Prupe.8G011800_v2.0.a1
pyrus_communis pycom16g08630
rosa_chinensis RchiOBHm_Chr5g0016311
rosa_laevigata RLG00000006792
rosa_multiflora Rmu_co8454895.1_g000001 Rmu_sc0001762.1_g000036
rosa_roxburghii Rroxscaffold_5G00374460
rosa_samantha Rh1AG277500 Rh4AG318700 Rh4BG326800 Rh4CG342200 Rh4DG322000 Rh5CG136300
rosa_wichuraiana Rw4G022550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 307
AciI CCGC 1 cut(s) 80
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 2 cut(s) 52, 512
AcuI CTGAAG 2 cut(s) 297, 467
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 2 cut(s) 216, 293
AfeI AGCGCT 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 6 cut(s) 99, 111, 130, 265, 331, 350
AluBI AGCT 1 cut(s) 325
AluI AGCT 1 cut(s) 325
Aor51HI AGCGCT 1 cut(s) 452
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 2 cut(s) 52, 512
AspLEI GCGC 1 cut(s) 453
AsuHPI GGTGA 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 219
BalI TGGCCA 1 cut(s) 20
BccI CCATC 2 cut(s) 125, 399
BfmI CTRYAG 1 cut(s) 300
BfoI RGCGCY 1 cut(s) 454
BfuAI ACCTGC 1 cut(s) 307
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
BmcAI AGTACT 1 cut(s) 293
BmsI GCATC 2 cut(s) 14, 409
BpuEI CTTGAG 1 cut(s) 326
BsaAI YACGTR 2 cut(s) 460, 565
BsaJI CCNNGG 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse21I CCTNAGG 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 1 cut(s) 5
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMII CTCAG 3 cut(s) 104, 168, 210
BshFI GGCC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 396
BsnI GGCC 1 cut(s) 20
Bsp143I GATC 2 cut(s) 198, 304
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 1 cut(s) 80
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 3 cut(s) 103, 169, 211
BspMI ACCTGC 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 2 cut(s) 198, 304
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 291, 526
BstBAI YACGTR 2 cut(s) 460, 565
BstC8I GCNNGC 1 cut(s) 455
BstDEI CTNAG 3 cut(s) 90, 177, 219
BstDSI CCRYGG 1 cut(s) 208
BstEII GGTNACC 1 cut(s) 364
BstF5I GGATG 1 cut(s) 5
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 1 cut(s) 453
BstKTI GATC 2 cut(s) 201, 307
BstMBI GATC 2 cut(s) 198, 304
BstMWI GCNNNNNNNGC 2 cut(s) 322, 559
BstPI GGTNACC 1 cut(s) 364
BstSFI CTRYAG 1 cut(s) 300
Bsu36I CCTNAGG 1 cut(s) 219
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 208
BtgZI GCGATG 1 cut(s) 386
BtsCI GGATG 1 cut(s) 5
BtsIMutI CAGTG 1 cut(s) 141
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 455
CfoI GCGC 1 cut(s) 453
Csp6I GTAC 2 cut(s) 215, 292
CviAII CATG 4 cut(s) 209, 370, 532, 536
CviJI RGCY 5 cut(s) 20, 79, 189, 325, 553
CviKI_1 RGCY 5 cut(s) 20, 79, 189, 325, 553
CviQI GTAC 2 cut(s) 215, 292
DdeI CTNAG 3 cut(s) 90, 177, 219
DpnI GATC 2 cut(s) 200, 306
DpnII GATC 2 cut(s) 198, 304
DraIII CACNNNGTG 1 cut(s) 372
EaeI YGGCCR 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 208
Eco32I GATATC 1 cut(s) 165
Eco47III AGCGCT 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 297, 467
Eco81I CCTNAGG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 364
EcoRV GATATC 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 208
EcoT22I ATGCAT 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 4 cut(s) 212, 373, 535, 539
FaiI YATR 9 cut(s) 138, 186, 210, 229, 252, 371, 491, 533, 537
FatI CATG 4 cut(s) 208, 369, 531, 535
Fnu4HI GCNGC 1 cut(s) 80
Fsp4HI GCNGC 1 cut(s) 80
GlaI GCGC 1 cut(s) 452
GluI GCNGC 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 1 cut(s) 20
HhaI GCGC 1 cut(s) 453
Hin1II CATG 4 cut(s) 212, 373, 535, 539
Hin6I GCGC 1 cut(s) 451
HinP1I GCGC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 3 cut(s) 70, 107, 346
HphI GGTGA 1 cut(s) 358
Hpy188I TCNGA 5 cut(s) 93, 159, 178, 447, 472
Hpy188III TCNNGA 3 cut(s) 196, 343, 356
HpyAV CCTTC 2 cut(s) 19, 394
HpyCH4III ACNGT 2 cut(s) 291, 526
HpyCH4IV ACGT 2 cut(s) 459, 564
HpyCH4V TGCA 2 cut(s) 457, 535
HpyF10VI GCNNNNNNNGC 2 cut(s) 322, 559
HpyF3I CTNAG 3 cut(s) 90, 177, 219
HpySE526I ACGT 2 cut(s) 459, 564
Hsp92II CATG 4 cut(s) 212, 373, 535, 539
HspAI GCGC 1 cut(s) 451
Kzo9I GATC 2 cut(s) 198, 304
LpnPI CCDG 6 cut(s) 78, 209, 231, 302, 347, 432
LweI GCATC 2 cut(s) 14, 409
MaeII ACGT 2 cut(s) 459, 564
MaeIII GTNAC 5 cut(s) 71, 364, 414, 479, 520
MalI GATC 2 cut(s) 200, 306
MboI GATC 2 cut(s) 198, 304
MboII GAAGA 3 cut(s) 172, 253, 290
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 7 cut(s) 52, 111, 257, 503, 512, 544, 568
MluNI TGGCCA 1 cut(s) 20
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 7 cut(s) 45, 95, 172, 214, 350, 438, 453
Mox20I TGGCCA 1 cut(s) 20
Mph1103I ATGCAT 1 cut(s) 537
MscI TGGCCA 1 cut(s) 20
Msp20I TGGCCA 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 322, 559
NcoI CCATGG 1 cut(s) 208
NdeII GATC 2 cut(s) 198, 304
NlaIII CATG 4 cut(s) 212, 373, 535, 539
NmuCI GTSAC 2 cut(s) 71, 364
NsiI ATGCAT 1 cut(s) 537
PfeI GAWTC 2 cut(s) 107, 346
PkrI GCNGC 1 cut(s) 81
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
Ppu21I YACGTR 2 cut(s) 460, 565
PspEI GGTNACC 1 cut(s) 364
RsaI GTAC 2 cut(s) 216, 293
RsaNI GTAC 2 cut(s) 215, 292
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 2 cut(s) 198, 304
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 1 cut(s) 79
SfaNI GCATC 2 cut(s) 14, 409
SfcI CTRYAG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 7 cut(s) 52, 111, 257, 503, 512, 544, 568
SsiI CCGC 1 cut(s) 80
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 2 cut(s) 291, 526
TaiI ACGT 2 cut(s) 462, 567
TasI AATT 7 cut(s) 52, 111, 257, 503, 512, 544, 568
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 2 cut(s) 107, 346
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 2 cut(s) 71, 364
Tsp45I GTSAC 2 cut(s) 71, 364
TspDTI ATGAA 1 cut(s) 46
TspRI CASTG 1 cut(s) 148
XapI RAATTY 2 cut(s) 52, 512
ZrmI AGTACT 1 cut(s) 293
Zsp2I ATGCAT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.