Rh4DG322000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014722
Physical Location & Seq
Forward (+)
54543596 .. 54545675
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG322000.1

Sequence Viewer

Length: 444 bp
ATGATCGAGTGTGTTTCACATGTTGCCATTCATTTAGACTCTCAAACTCACCTATTCTCAAGCATGACATCCTTCCAAATGGCCAATGACATCAACCCTCTTTTCCTTTCCCCAAATTTCATTCTCCCAGAGAGTCACAGGCCGCACCTCACTCAGATTTCAACTTTGGATTCAATTCTCATCATTGACTTGAATGACTATGACACTGACAACTTGGTTCAGAAGATATTACAAGCTTCTGAGGTCTATGGCTTTTTCCAGATCATCAACCATGGAGCACCTGAGGAAATATGCGATAGAATGATGACTACCTATACACAATTCTTCAAACTTTCTGCTGAAGAAAAGGCACAGTACTTCACTACAGATCACACCAAGCAGGTCAAGCTTTTCAACCTACTTCCTCAAGATTCAAGGTCAAGAACAGGTCACCATGTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

147

Amino Acids

16.94

Weight (kDa)

5.33

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 61 - 125 5.3e-16 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014722)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25590
malus_domestica MD16G1101600.v1.1 MD16G1101900.v1.1
prunus_persica Prupe.8G011800_v2.0.a1
pyrus_communis pycom16g08630
rosa_chinensis RchiOBHm_Chr5g0016311
rosa_laevigata RLG00000006792
rosa_multiflora Rmu_co8454895.1_g000001 Rmu_sc0001762.1_g000036
rosa_roxburghii Rroxscaffold_5G00374460
rosa_samantha Rh1AG277500 Rh4AG318700 Rh4BG326800 Rh4CG342200 Rh4DG322000 Rh5CG136300
rosa_wichuraiana Rw4G022550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 370
AciI CCGC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 115
AcuI CTGAAG 1 cut(s) 360
AdeI CACNNNGTG 1 cut(s) 436
AfaI GTAC 1 cut(s) 356
AflIII ACRYGT 1 cut(s) 19
AgsI TTSAA 6 cut(s) 162, 174, 193, 328, 394, 414
AluBI AGCT 2 cut(s) 236, 388
AluI AGCT 2 cut(s) 236, 388
Alw21I GWGCWC 1 cut(s) 280
AoxI GGCC 2 cut(s) 81, 140
ApoI RAATTY 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 41, 422
AxyI CCTNAGG 1 cut(s) 282
BalI TGGCCA 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 280
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 370
BisI GCNGC 1 cut(s) 143
BlsI GCNGC 1 cut(s) 144
BmcAI AGTACT 1 cut(s) 356
BpuEI CTTGAG 2 cut(s) 43, 390
BsaJI CCNNGG 1 cut(s) 271
Bse21I CCTNAGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 1 cut(s) 68
BseMII CTCAG 3 cut(s) 167, 231, 273
BshFI GGCC 2 cut(s) 83, 142
BsiHKAI GWGCWC 1 cut(s) 280
BsnI GGCC 2 cut(s) 83, 142
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 3 cut(s) 3, 261, 367
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 1 cut(s) 143
BspANI GGCC 2 cut(s) 83, 142
BspCNI CTCAG 3 cut(s) 166, 232, 274
BspMI ACCTGC 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 3 cut(s) 3, 261, 367
BssT1I CCWWGG 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 354
BstDEI CTNAG 3 cut(s) 153, 240, 282
BstDSI CCRYGG 1 cut(s) 271
BstEII GGTNACC 1 cut(s) 428
BstF5I GGATG 1 cut(s) 68
BstKTI GATC 3 cut(s) 6, 264, 370
BstMBI GATC 3 cut(s) 3, 261, 367
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstNSI RCATGY 1 cut(s) 23
BstPI GGTNACC 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 363
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 2 cut(s) 83, 142
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 68
BtsIMutI CAGTG 1 cut(s) 204
BveI ACCTGC 1 cut(s) 370
Csp6I GTAC 1 cut(s) 355
CviAII CATG 4 cut(s) 20, 64, 272, 434
CviJI RGCY 5 cut(s) 83, 142, 236, 252, 388
CviKI_1 RGCY 5 cut(s) 83, 142, 236, 252, 388
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 3 cut(s) 153, 240, 282
DpnI GATC 3 cut(s) 5, 263, 369
DpnII GATC 3 cut(s) 3, 261, 367
DraIII CACNNNGTG 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 360
Eco81I CCTNAGG 1 cut(s) 282
Eco91I GGTNACC 1 cut(s) 428
EcoO65I GGTNACC 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 4 cut(s) 23, 67, 275, 437
FaiI YATR 8 cut(s) 21, 65, 201, 249, 273, 292, 315, 435
FatI CATG 4 cut(s) 19, 63, 271, 433
Fnu4HI GCNGC 1 cut(s) 143
FokI GGATG 1 cut(s) 55
Fsp4HI GCNGC 1 cut(s) 143
GluI GCNGC 1 cut(s) 143
HaeIII GGCC 2 cut(s) 83, 142
Hin1II CATG 4 cut(s) 23, 67, 275, 437
HindIII AAGCTT 2 cut(s) 234, 386
HinfI GANTC 4 cut(s) 38, 133, 170, 410
HphI GGTGA 2 cut(s) 41, 422
Hpy188I TCNGA 3 cut(s) 156, 222, 241
Hpy188III TCNNGA 3 cut(s) 259, 407, 420
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
HpyF3I CTNAG 3 cut(s) 153, 240, 282
Hsp92II CATG 4 cut(s) 23, 67, 275, 437
Kzo9I GATC 3 cut(s) 3, 261, 367
LmnI GCTCC 1 cut(s) 275
LpnPI CCDG 6 cut(s) 124, 141, 272, 294, 365, 411
MaeIII GTNAC 2 cut(s) 134, 428
MalI GATC 3 cut(s) 5, 263, 369
MboI GATC 3 cut(s) 3, 261, 367
MboII GAAGA 3 cut(s) 235, 316, 353
MhlI GDGCHC 1 cut(s) 280
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 3 cut(s) 115, 174, 320
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 2 cut(s) 32, 142
MnlI CCTC 5 cut(s) 108, 158, 235, 277, 414
Mox20I TGGCCA 1 cut(s) 83
MscI TGGCCA 1 cut(s) 83
Msp20I TGGCCA 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 385
NcoI CCATGG 1 cut(s) 271
NdeII GATC 3 cut(s) 3, 261, 367
NlaIII CATG 4 cut(s) 23, 67, 275, 437
NmuCI GTSAC 2 cut(s) 134, 428
NspI RCATGY 1 cut(s) 23
PciI ACATGT 1 cut(s) 19
PfeI GAWTC 2 cut(s) 170, 410
PkrI GCNGC 1 cut(s) 144
PleI GAGTC 2 cut(s) 32, 141
PpsI GAGTC 2 cut(s) 32, 141
PscI ACATGT 1 cut(s) 19
PspEI GGTNACC 1 cut(s) 428
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SatI GCNGC 1 cut(s) 143
Sau3AI GATC 3 cut(s) 3, 261, 367
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 2 cut(s) 32, 142
SduI GDGCHC 1 cut(s) 280
SfcI CTRYAG 1 cut(s) 363
SmlI CTYRAG 2 cut(s) 58, 405
SmoI CTYRAG 2 cut(s) 58, 405
Sse9I AATT 3 cut(s) 115, 174, 320
SsiI CCGC 1 cut(s) 143
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 1 cut(s) 354
TaqI TCGA 1 cut(s) 6
TasI AATT 3 cut(s) 115, 174, 320
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 2 cut(s) 170, 410
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 2 cut(s) 134, 428
Tsp45I GTSAC 2 cut(s) 134, 428
TspDTI ATGAA 2 cut(s) 20, 109
TspRI CASTG 1 cut(s) 211
XapI RAATTY 1 cut(s) 115
XceI RCATGY 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.