Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0006525

Physical Location & Seq

Genomic Coordinates Forward (+)

27482105 .. 27486389

Gene Structure 4285 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g25820.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1380 bp

ATGGTCGTCGTGTGTTGGAAGAGCAATCGATTGTGTTATTTTGATGACACAAGAAGACAATTACCACATCAACGTAAACAACAAAGGGATTCAACACAAGAAACAGGACAAGAAAATTTCACAGCAGCCATGGCTTCTAGCAGCTGGTCTATGATTTCCTCCCCAATCATTCCTCTTCTTGTTTGCTTCCTTCTTGTTTCTTCTTCAATATGTCAAGTAGTCCACGCCTCCGAAATGGCCAATCAGACTTTCAATCCCGAGCAACAGTTAAAGAAGTTGAAGAACATGAAGGCTCGTCTCAGAAGAATCAACAAGCCTGCCCTCAAGACATTTCAGAGTCCAGATGGTGATCTCATAGATTGTGTTCACTCTCATCACCAACCTGCTTTTGATCATCCTCGATTAAAAGGACAGAAGCCATTGGATCCACCGGAGAGACCAAAAGGACTTAACCCCACTAGCATGATCCATGAAAATTTTCAGCTATGGAGCTTATCAGGTAATATATGCCCAGATGGAACGATTCCGATTCGAAGAACAAGAGAGCAAGACGTGCTCAGAGCTAGCTCGGTTCGACGATACGGAAGAAAATCACCAAGGCATGTTCGAAGAGATACATCTAGCAATGGCCATGAGCATGCAGTTGGGTATGTGAGTGGAGATCAATACTATGGAGCAAAAGCAAGCATCAATGTATGGGCACCAAAGGTTGTGAATCAATATGAATTCAGCTTATCTCAAATGTGGGTTATTTCTGGTTCATTTGGCGACGATCTCAACACCATTGAAGCTGGTTGGCAGGTCAGCCCAGAGCTATATGGGGACAACTATCCTAGATTCTTTACTTACTGGACTTCAGATGCCTATCAAGCAACTGGGTGCTACAATCTGCTGTGTTCGGGTTTTGTTCAGACGAATAGTCGAATTGCCATTGGAGCTGCAATTTCTCCGACTTCTGGTTACAATGGTGGACAGTTTGATATTAGCTTATTGATTTGGAAGGATCCAAAGCATGGAAATTGGTGGCTGGAATTTGGGAATGGGGTTCTAGTTGGATACTGGCCATCATTCTTGTTCACTCATCTTCAAAACCACGCAAGCATGGTACAATTTGGTGGAGAAGTTGTAAACTCAAGGCCTTCTGGATCTCACACTGCAACACAAATGGGAAGTGGTCATTTTGCAGGAGAAGGCTTTGGCAAAGCTTCTTACTTTAGAAACTTGCAGGTTGTGGATTGGGACAACAGCCTAATCCCATTATCAAACCTCAAAGTTTTGGCAGATCATCCAAATTGCTATGACATCCAAGGAGGGATTAACAATTTTTGGGGGAATTATTTTTACTATGGTGGTCCTGGAAGAAATGTGAGGTGTCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Neprosin Neprosin_AP

Protein Analysis

460

Amino Acids

51.69

Weight (kDa)

8.89

Isoelectric Point (pI)

33.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Neprosin_AP	PF14365	110 - 217	1.1e-37	Neprosin activation peptide
Neprosin	PF03080	241 - 452	5.3e-87	Neprosin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006525)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10750 AT1G10750 AT1G23340 AT1G23340 AT1G23340 AT1G70550 AT1G70550
fragaria_vesca	FvH4_4g25820 FvH4_4g25820 FvH4_4g25820 FvH4_4g25820
malus_domestica	MD13G1097000.v1.1 MD16G1097900.v1.1
prunus_persica	Prupe.1G248600_v2.0.a1
pyrus_communis	pycom13g08470 pycom16g08340
rosa_chinensis	RchiOBHm_Chr4g0433271
rosa_laevigata	RLG00000006768
rosa_multiflora	Rmu_sc0022950.1_g000002
rosa_roxburghii	Rroxscaffold_5G00374830
rosa_rugosa	Rorug04G0267600
rosa_samantha	Rh4AG322100 Rh4BG329500 Rh4CG344800 Rh4DG326200
rosa_wichuraiana	Rw4G027870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	391, 790, 1216
AccB1I	GGYRCC	1 cut(s)	700
AccB7I	CCANNNNNTGG	1 cut(s)	1013
AclWI	GGATC	6 cut(s)	419, 432, 460, 998, 1011, 1153
AcoI	YGGCCR	3 cut(s)	237, 628, 1061
AcsI	RAATTY	4 cut(s)	115, 475, 725, 1031
AcuI	CTGAAG	1 cut(s)	840
AfaI	GTAC	1 cut(s)	1107
AfiI	CCNNNNNNNGG	2 cut(s)	956, 1013
AgsI	TTSAA	6 cut(s)	93, 207, 253, 280, 788, 1088
AhdI	GACNNNNNGTC	1 cut(s)	918
AjiI	CACGTC	1 cut(s)	553
AjnI	CCWGG	1 cut(s)	1354
AjuI	GAANNNNNNNTTGG	2 cut(s)	233, 265
Alw21I	GWGCWC	1 cut(s)	558
Alw26I	GTCTC	2 cut(s)	302, 430
AlwI	GGATC	6 cut(s)	419, 432, 460, 998, 1011, 1153
Ama87I	CYCGRG	1 cut(s)	257
AoxI	GGCC	4 cut(s)	237, 628, 1061, 1136
ApeKI	GCWGC	3 cut(s)	125, 141, 938
ApoI	RAATTY	4 cut(s)	115, 475, 725, 1031
Asp700I	GAANNNNTTC	1 cut(s)	477
AspS9I	GGNCC	1 cut(s)	1352
AsuHPI	GGTGA	3 cut(s)	359, 368, 585
AsuII	TTCGAA	2 cut(s)	532, 607
AsuNHI	GCTAGC	1 cut(s)	563
AvaI	CYCGRG	1 cut(s)	257
AvaII	GGWCC	1 cut(s)	1352
BaeGI	GKGCMC	1 cut(s)	703
BalI	TGGCCA	3 cut(s)	239, 630, 1063
BamHI	GGATCC	2 cut(s)	424, 1003
BanI	GGYRCC	1 cut(s)	700
BbsI	GAAGAC	1 cut(s)	61
Bbv12I	GWGCWC	1 cut(s)	558
BbvI	GCAGC	3 cut(s)	137, 153, 925
BccI	CCATC	3 cut(s)	338, 509, 1072
BciT130I	CCWGG	1 cut(s)	1356
BciVI	GTATCC	1 cut(s)	1049
BclI	TGATCA	1 cut(s)	391
BcoDI	GTCTC	2 cut(s)	302, 430
BfaI	CTAG	6 cut(s)	138, 459, 564, 621, 834, 1049
BfuAI	ACCTGC	3 cut(s)	391, 790, 1216
BfuI	GTATCC	1 cut(s)	1049
BisI	GCNGC	3 cut(s)	126, 142, 939
BlsI	GCNGC	3 cut(s)	127, 143, 940
Bme1390I	CCNGG	1 cut(s)	1356
Bme18I	GGWCC	1 cut(s)	1352
BmeRI	GACNNNNNGTC	1 cut(s)	918
BmeT110I	CYCGRG	1 cut(s)	257
BmgBI	CACGTC	1 cut(s)	553
BmgT120I	GGNCC	1 cut(s)	1352
BmiI	GGNNCC	3 cut(s)	426, 702, 1005
BmrFI	CCNGG	1 cut(s)	1356
BmrI	ACTGGG	1 cut(s)	885
BmsI	GCATC	2 cut(s)	696, 850
BmtI	GCTAGC	1 cut(s)	567
BmuI	ACTGGG	1 cut(s)	885
BpiI	GAAGAC	1 cut(s)	61
Bpu14I	TTCGAA	2 cut(s)	532, 607
BpuEI	CTTGAG	2 cut(s)	308, 1117
Bsa29I	ATCGAT	1 cut(s)	28
BsaBI	GATNNNNATC	2 cut(s)	348, 864
BsaI	GGTCTC	1 cut(s)	430
BsaJI	CCNNGG	3 cut(s)	129, 596, 1306
BsaWI	WCCGGW	1 cut(s)	430
BsaXI	ACNNNNNCTCC	2 cut(s)	1110, 1140
Bsc4I	CCNNNNNNNGG	2 cut(s)	956, 1013
Bse1I	ACTGG	3 cut(s)	854, 880, 1064
Bse3DI	GCAATG	1 cut(s)	631
Bse8I	GATNNNNATC	2 cut(s)	348, 864
BseBI	CCWGG	1 cut(s)	1356
BseCI	ATCGAT	1 cut(s)	28
BseDI	CCNNGG	3 cut(s)	129, 596, 1306
BseGI	GGATG	3 cut(s)	394, 1285, 1302
BseJI	GATNNNNATC	2 cut(s)	348, 864
BseLI	CCNNNNNNNGG	2 cut(s)	956, 1013
BseMI	GCAATG	1 cut(s)	631
BseMII	CTCAG	2 cut(s)	313, 571
BseNI	ACTGG	3 cut(s)	854, 880, 1064
BseSI	GKGCMC	1 cut(s)	703
BseXI	GCAGC	3 cut(s)	137, 153, 925
BshFI	GGCC	4 cut(s)	239, 630, 1063, 1138
BshNI	GGYRCC	1 cut(s)	700
BshVI	ATCGAT	1 cut(s)	28
BsiHKAI	GWGCWC	1 cut(s)	558
BsiHKCI	CYCGRG	1 cut(s)	257
BsiSI	CCGG	1 cut(s)	431
BslFI	GGGAC	3 cut(s)	836, 1253, 1359
BslI	CCNNNNNNNGG	2 cut(s)	956, 1013
BsmAI	GTCTC	2 cut(s)	302, 430
BsmBI	CGTCTC	1 cut(s)	302
BsmFI	GGGAC	3 cut(s)	836, 1253, 1359
BsnI	GGCC	4 cut(s)	239, 630, 1063, 1138
Bso31I	GGTCTC	1 cut(s)	430
BsoBI	CYCGRG	1 cut(s)	257
Bsp119I	TTCGAA	2 cut(s)	532, 607
Bsp1286I	GDGCHC	2 cut(s)	558, 703
Bsp143I	GATC	9 cut(s)	349, 391, 424, 465, 661, 772, 1003, 1145, 1282
Bsp19I	CCATGG	1 cut(s)	129
BspANI	GGCC	4 cut(s)	239, 630, 1063, 1138
BspCNI	CTCAG	2 cut(s)	312, 570
BspDI	ATCGAT	1 cut(s)	28
BspLI	GGNNCC	3 cut(s)	426, 702, 1005
BspMI	ACCTGC	3 cut(s)	391, 790, 1216
BspOI	GCTAGC	1 cut(s)	567
BspPI	GGATC	6 cut(s)	419, 432, 460, 998, 1011, 1153
BspQI	GCTCTTC	1 cut(s)	14
BspT104I	TTCGAA	2 cut(s)	532, 607
BspT107I	GGYRCC	1 cut(s)	700
BspTNI	GGTCTC	1 cut(s)	430
BsrDI	GCAATG	1 cut(s)	631
BsrI	ACTGG	3 cut(s)	854, 880, 1064
BssECI	CCNNGG	3 cut(s)	129, 596, 1306
BssMI	GATC	9 cut(s)	349, 391, 424, 465, 661, 772, 1003, 1145, 1282
BssT1I	CCWWGG	3 cut(s)	129, 596, 1306
Bst2UI	CCWGG	1 cut(s)	1356
Bst4CI	ACNGT	2 cut(s)	267, 975
Bst6I	CTCTTC	3 cut(s)	14, 180, 604
BstAPI	GCANNNNNTGC	1 cut(s)	553
BstBI	TTCGAA	2 cut(s)	532, 607
BstC8I	GCNNGC	5 cut(s)	318, 565, 639, 685, 1099
BstDEI	CTNAG	2 cut(s)	299, 557
BstDSI	CCRYGG	1 cut(s)	129
BstF5I	GGATG	3 cut(s)	394, 1285, 1302
BstKTI	GATC	9 cut(s)	352, 394, 427, 468, 664, 775, 1006, 1148, 1285
BstMAI	GTCTC	2 cut(s)	302, 430
BstMBI	GATC	9 cut(s)	349, 391, 424, 465, 661, 772, 1003, 1145, 1282
BstMWI	GCNNNNNNNGC	4 cut(s)	131, 553, 869, 935
BstNI	CCWGG	1 cut(s)	1356
BstNSI	RCATGY	2 cut(s)	605, 641
BstSCI	CCNGG	1 cut(s)	1354
BstSLI	GKGCMC	1 cut(s)	703
BstV1I	GCAGC	3 cut(s)	137, 153, 925
BstV2I	GAAGAC	1 cut(s)	61
BstX2I	RGATCY	3 cut(s)	424, 1003, 1145
BstYI	RGATCY	3 cut(s)	424, 1003, 1145
Bsu15I	ATCGAT	1 cut(s)	28
BsuI	GTATCC	1 cut(s)	1049
BsuRI	GGCC	4 cut(s)	239, 630, 1063, 1138
BsuTUI	ATCGAT	1 cut(s)	28
BtgI	CCRYGG	1 cut(s)	129
BtrI	CACGTC	1 cut(s)	553
BtsCI	GGATG	3 cut(s)	394, 1285, 1302
BtsI	GCAGTG	1 cut(s)	1152
BtsIMutI	CAGTG	1 cut(s)	1152
BveI	ACCTGC	3 cut(s)	391, 790, 1216
Cac8I	GCNNGC	5 cut(s)	318, 565, 639, 685, 1099
Cfr13I	GGNCC	1 cut(s)	1352
ClaI	ATCGAT	1 cut(s)	28
Csp6I	GTAC	1 cut(s)	1106
CviAII	CATG	9 cut(s)	130, 286, 463, 470, 602, 632, 638, 1013, 1102
CviQI	GTAC	1 cut(s)	1106
DdeI	CTNAG	2 cut(s)	299, 557
DpnI	GATC	9 cut(s)	351, 393, 426, 467, 663, 774, 1005, 1147, 1284
DpnII	GATC	9 cut(s)	349, 391, 424, 465, 661, 772, 1003, 1145, 1282
DriI	GACNNNNNGTC	1 cut(s)	918
EaeI	YGGCCR	3 cut(s)	237, 628, 1061
Eam1104I	CTCTTC	3 cut(s)	14, 180, 604
Eam1105I	GACNNNNNGTC	1 cut(s)	918
EarI	CTCTTC	3 cut(s)	14, 180, 604
Eco130I	CCWWGG	3 cut(s)	129, 596, 1306
Eco147I	AGGCCT	1 cut(s)	1138
Eco31I	GGTCTC	1 cut(s)	430
Eco47I	GGWCC	1 cut(s)	1352
Eco57I	CTGAAG	1 cut(s)	840
Eco88I	CYCGRG	1 cut(s)	257
EcoRI	GAATTC	1 cut(s)	725
EcoRII	CCWGG	1 cut(s)	1354
EcoT14I	CCWWGG	3 cut(s)	129, 596, 1306
ErhI	CCWWGG	3 cut(s)	129, 596, 1306
Esp3I	CGTCTC	1 cut(s)	302
FaeI	CATG	9 cut(s)	133, 289, 466, 473, 605, 635, 641, 1016, 1105
FaqI	GGGAC	3 cut(s)	836, 1253, 1359
FatI	CATG	9 cut(s)	129, 285, 462, 469, 601, 631, 637, 1012, 1101
FbaI	TGATCA	1 cut(s)	391
Fnu4HI	GCNGC	3 cut(s)	126, 142, 939
FokI	GGATG	3 cut(s)	381, 1272, 1289
Fsp4HI	GCNGC	3 cut(s)	126, 142, 939
FspBI	CTAG	6 cut(s)	138, 459, 564, 621, 834, 1049
GluI	GCNGC	3 cut(s)	126, 142, 939
HaeIII	GGCC	4 cut(s)	239, 630, 1063, 1138
HapII	CCGG	1 cut(s)	431
Hin1II	CATG	9 cut(s)	133, 289, 466, 473, 605, 635, 641, 1016, 1105
HindIII	AAGCTT	1 cut(s)	1203
HinfI	GANTC	7 cut(s)	89, 306, 337, 523, 529, 715, 837
HpaII	CCGG	1 cut(s)	431
HphI	GGTGA	3 cut(s)	359, 368, 585
Hpy166II	GTNNAC	6 cut(s)	77, 223, 367, 971, 1077, 1129
Hpy188I	TCNGA	9 cut(s)	232, 246, 302, 336, 528, 560, 859, 912, 951
Hpy188III	TCNNGA	4 cut(s)	257, 325, 341, 1143
Hpy8I	GTNNAC	6 cut(s)	77, 223, 367, 971, 1077, 1129
Hpy99I	CGWCG	3 cut(s)	11, 579, 773
HpyAV	CCTTC	5 cut(s)	200, 283, 994, 1149, 1184
HpyCH4III	ACNGT	2 cut(s)	267, 975
HpyCH4IV	ACGT	2 cut(s)	73, 552
HpyCH4V	TGCA	5 cut(s)	641, 941, 1157, 1184, 1225
HpyF10VI	GCNNNNNNNGC	4 cut(s)	131, 553, 869, 935
HpyF3I	CTNAG	2 cut(s)	299, 557
HpySE526I	ACGT	2 cut(s)	73, 552
Hsp92II	CATG	9 cut(s)	133, 289, 466, 473, 605, 635, 641, 1016, 1105
Ksp22I	TGATCA	1 cut(s)	391
Kzo9I	GATC	9 cut(s)	349, 391, 424, 465, 661, 772, 1003, 1145, 1282
LguI	GCTCTTC	1 cut(s)	14
LmnI	GCTCC	3 cut(s)	489, 674, 935
Lsp1109I	GCAGC	3 cut(s)	137, 153, 925
LweI	GCATC	2 cut(s)	696, 850
MaeI	CTAG	6 cut(s)	138, 459, 564, 621, 834, 1049
MaeII	ACGT	2 cut(s)	73, 552
MaeIII	GTNAC	1 cut(s)	959
MalI	GATC	9 cut(s)	351, 393, 426, 467, 663, 774, 1005, 1147, 1284
MboI	GATC	9 cut(s)	349, 391, 424, 465, 661, 772, 1003, 1145, 1282
MflI	RGATCY	3 cut(s)	424, 1003, 1145
MhlI	GDGCHC	2 cut(s)	558, 703
MlsI	TGGCCA	3 cut(s)	239, 630, 1063
MluNI	TGGCCA	3 cut(s)	239, 630, 1063
MlyI	GAGTC	1 cut(s)	346
MmeI	TCCRAC	2 cut(s)	974, 1033
MnlI	CCTC	8 cut(s)	169, 183, 238, 332, 408, 1277, 1304, 1362
Mox20I	TGGCCA	3 cut(s)	239, 630, 1063
MroXI	GAANNNNTTC	1 cut(s)	477
MscI	TGGCCA	3 cut(s)	239, 630, 1063
MseI	TTAA	4 cut(s)	269, 404, 450, 1317
MslI	CAYNNNNRTG	2 cut(s)	461, 636
Msp20I	TGGCCA	3 cut(s)	239, 630, 1063
MspA1I	CMGCKG	1 cut(s)	144
MspI	CCGG	1 cut(s)	431
MspR9I	CCNGG	1 cut(s)	1356
MvaI	CCWGG	1 cut(s)	1356
MwoI	GCNNNNNNNGC	4 cut(s)	131, 553, 869, 935
NcoI	CCATGG	1 cut(s)	129
NdeII	GATC	9 cut(s)	349, 391, 424, 465, 661, 772, 1003, 1145, 1282
NheI	GCTAGC	1 cut(s)	563
NlaIII	CATG	9 cut(s)	133, 289, 466, 473, 605, 635, 641, 1016, 1105
NlaIV	GGNNCC	3 cut(s)	426, 702, 1005
NspI	RCATGY	2 cut(s)	605, 641
NspV	TTCGAA	2 cut(s)	532, 607
PaeI	GCATGC	1 cut(s)	641
PceI	AGGCCT	1 cut(s)	1138
PciSI	GCTCTTC	1 cut(s)	14
PdmI	GAANNNNTTC	1 cut(s)	477
PfeI	GAWTC	6 cut(s)	89, 306, 523, 529, 715, 837
PflMI	CCANNNNNTGG	1 cut(s)	1013
PfoI	TCCNGGA	1 cut(s)	1354
PkrI	GCNGC	3 cut(s)	127, 143, 940
PleI	GAGTC	1 cut(s)	345
PpsI	GAGTC	1 cut(s)	345
Psp6I	CCWGG	1 cut(s)	1354
PspGI	CCWGG	1 cut(s)	1354
PspN4I	GGNNCC	3 cut(s)	426, 702, 1005
PspPI	GGNCC	1 cut(s)	1352
PsuI	RGATCY	3 cut(s)	424, 1003, 1145
PvuII	CAGCTG	1 cut(s)	144
RsaI	GTAC	1 cut(s)	1107
RsaNI	GTAC	1 cut(s)	1106
RseI	CAYNNNNRTG	2 cut(s)	461, 636
SapI	GCTCTTC	1 cut(s)	14
SaqAI	TTAA	4 cut(s)	269, 404, 450, 1317
SatI	GCNGC	3 cut(s)	126, 142, 939
Sau3AI	GATC	9 cut(s)	349, 391, 424, 465, 661, 772, 1003, 1145, 1282
Sau96I	GGNCC	1 cut(s)	1352
SchI	GAGTC	1 cut(s)	346
ScrFI	CCNGG	1 cut(s)	1356
SduI	GDGCHC	2 cut(s)	558, 703
SfaNI	GCATC	2 cut(s)	696, 850
SfuI	TTCGAA	2 cut(s)	532, 607
SinI	GGWCC	1 cut(s)	1352
SmiMI	CAYNNNNRTG	2 cut(s)	461, 636
SmlI	CTYRAG	2 cut(s)	323, 1132
SmoI	CTYRAG	2 cut(s)	323, 1132
SphI	GCATGC	1 cut(s)	641
SseBI	AGGCCT	1 cut(s)	1138
SspMI	CTAG	6 cut(s)	138, 459, 564, 621, 834, 1049
StuI	AGGCCT	1 cut(s)	1138
StyD4I	CCNGG	1 cut(s)	1354
StyI	CCWWGG	3 cut(s)	129, 596, 1306
TaaI	ACNGT	2 cut(s)	267, 975
TaiI	ACGT	2 cut(s)	76, 555
TaqI	TCGA	6 cut(s)	28, 400, 532, 574, 607, 922
TfiI	GAWTC	6 cut(s)	89, 306, 523, 529, 715, 837
Tru1I	TTAA	4 cut(s)	269, 404, 450, 1317
Tru9I	TTAA	4 cut(s)	269, 404, 450, 1317
TscAI	CASTG	1 cut(s)	1159
TseI	GCWGC	3 cut(s)	125, 141, 938
TspDTI	ATGAA	4 cut(s)	302, 486, 738, 750
TspGWI	ACGGA	1 cut(s)	597
TspRI	CASTG	1 cut(s)	1159
Van91I	CCANNNNNTGG	1 cut(s)	1013
VpaK11BI	GGWCC	1 cut(s)	1352
XapI	RAATTY	4 cut(s)	115, 475, 725, 1031
XceI	RCATGY	2 cut(s)	605, 641
XmnI	GAANNNNTTC	1 cut(s)	477
XspI	CTAG	6 cut(s)	138, 459, 564, 621, 834, 1049

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.