pycom16g08340

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006525
Physical Location & Seq
Forward (+)
5552671 .. 5556743
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08340.6

Sequence Viewer

Length: 1431 bp
ATGGTCACCATGTATTGGATACTGGCTTTGGTGGGAGCTGAGCTTCCATGGACAAAAAATAGCACCAATATTTTATGCTGCAAATTTCATGACTACAGAAGAAGACAATACCAACAACAACAACAAGAGGATTCAACAAAAGCAAAAAAACAAGAAAACTTCTCAAGCAATATGGCTTGTAGCTACTCCAAGATTTCCCCAATCATTTCCATTGTTATTTGCTTCCTTCTTGTTGCTTCTTCAATGTGTCCGGTAGCACAATCTTCTGATATCCGCCGCGAAACCGCCGCCAATCAGACGTTGAAGCCAGAGCAAGAATTGAAAAAGTTGAAGACTATAAGAGCACGTCTTAACAAGATCAACAAGCCTGCTCTCAAGACATTTCAGAGTCCGGATGGCGATCTTATAGATTGTGTTCTCTCTCATCACCAACCAGCTTTCGATCATCCCCAGTTAAAAGGACAGAAGCCATTGGATCCGCCGGACAGACCGAAAGGCCAAAACCCGCCAGGAATGGCCGCTGAAAATTACCAGCTGTGGAGCATGTCAGGAAATCTCTGCCCAGAAGGAACACTTCCAATTAGAAGAACAAGAGAGGAAGATATGATGAGAGCCAGCTCTGTTAGAAGATTTGGAAGAAAGCTAAGAAGGCATGTCAGAAGAGATACATCCAGTAATGGCCATGAGCATGCAGTTGGGTATGTGAGTGGAGATCAATACTATGGAGCCAAAGCTAGCATCAATGTCTGGGCCCCCAAAGTTGTTAATCAATATGAATTCAGCTTGTCCCAGATGTGGGTCATCTCTGGTTCATTTGGTGATGACCTCAACACCATTGAAGCTGGTTGGCAGGTCAGTCCAGAGCTGTATGGGGACAACTACCCTAGATTCTTTACTTATTGGACTACGGATGCATATCAAGCAACTGGATGCTACAATTTACTGTGTTCTGGATTTGTTCAGACCAATAGTAGAATTGCCATTGGAGCCGCAATTTCTCCAACTTCTTGCTACCATGGTGGACAATTTGATATTAGCTTGTTAATTTGGAAGGATCCAAAGCATGGAAATTGGTGGCTGGAATTCGGGAACGGAGTCCTAGTCGGATACTGGCCATCATTCTTGTTCACTCACCTTCAAAACCACGCAAGCATGGTACAATTTGGTGGAGAAGTTGTGAACTCGAGGCCTTCAGGCTCCCACACAGCCACACAGATGGGCAGTGGGCACTTTGCAGGTGAAGGGTTTGGAAAAGCTTCATATTTTCGAAACATGCAGGTTGTGGATTGGGATAATAGCTTAATCCCATTATCCAACCTCAAAGTTTTGGCTGATCATCCAAATTGCTATGATATTCAAGGGGGGATTAACAACGTTTGGGGGAATTATTTTTACTATGGAGGTCCTGGAAGAAATGTGAGGTGTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

477

Amino Acids

53.65

Weight (kDa)

8.86

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1226
Acc36I ACCTGC 3 cut(s) 841, 1226, 1267
AccB7I CCANNNNNTGG 2 cut(s) 15, 1064
AccII CGCG 1 cut(s) 279
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 8 cut(s) 274, 277, 285, 288, 479, 506, 519, 990
AclI AACGTT 1 cut(s) 1374
AclWI GGATC 4 cut(s) 470, 483, 1049, 1062
AcoI YGGCCR 3 cut(s) 516, 679, 1112
AcsI RAATTY 3 cut(s) 83, 776, 1082
AcuI CTGAAG 1 cut(s) 1176
AfaI GTAC 1 cut(s) 1158
AfiI CCNNNNNNNGG 4 cut(s) 15, 795, 796, 1064
AgsI TTSAA 8 cut(s) 135, 243, 304, 322, 331, 839, 1139, 1358
AjiI CACGTC 1 cut(s) 347
AjnI CCWGG 2 cut(s) 508, 1405
Alw21I GWGCWC 1 cut(s) 346
AlwI GGATC 4 cut(s) 470, 483, 1049, 1062
Ama87I CYCGRG 1 cut(s) 1183
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 6 cut(s) 496, 516, 679, 750, 1112, 1187
ApaI GGGCCC 1 cut(s) 754
ApeKI GCWGC 1 cut(s) 78
ApoI RAATTY 3 cut(s) 83, 776, 1082
Asp700I GAANNNNTTC 1 cut(s) 1255
AspS9I GGNCC 3 cut(s) 750, 751, 1403
AsuHPI GGTGA 4 cut(s) 419, 830, 1124, 1250
AsuII TTCGAA 1 cut(s) 1267
AsuNHI GCTAGC 1 cut(s) 734
AvaI CYCGRG 1 cut(s) 1183
AvaII GGWCC 1 cut(s) 1403
BaeGI GKGCMC 2 cut(s) 754, 1230
BalI TGGCCA 2 cut(s) 681, 1114
BamHI GGATCC 2 cut(s) 475, 1054
BanII GRGCYC 1 cut(s) 754
BbsI GAAGAC 2 cut(s) 109, 338
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 1 cut(s) 65
BccI CCATC 3 cut(s) 389, 1123, 1210
BciT130I CCWGG 2 cut(s) 510, 1407
BciVI GTATCC 2 cut(s) 12, 1100
BclI TGATCA 1 cut(s) 1333
BfaI CTAG 3 cut(s) 735, 885, 1100
BfmI CTRYAG 1 cut(s) 94
BfuAI ACCTGC 3 cut(s) 841, 1226, 1267
BfuI GTATCC 2 cut(s) 12, 1100
BisI GCNGC 5 cut(s) 79, 277, 288, 519, 990
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 5 cut(s) 80, 278, 289, 520, 991
Bme1390I CCNGG 2 cut(s) 510, 1407
Bme18I GGWCC 1 cut(s) 1403
BmeT110I CYCGRG 1 cut(s) 1183
BmgBI CACGTC 1 cut(s) 347
BmgT120I GGNCC 3 cut(s) 750, 751, 1403
BmiI GGNNCC 7 cut(s) 477, 727, 752, 753, 988, 1056, 1198
BmrFI CCNGG 2 cut(s) 510, 1407
BmrI ACTGGG 1 cut(s) 445
BmsI GCATC 3 cut(s) 747, 901, 920
BmtI GCTAGC 1 cut(s) 738
BmuI ACTGGG 1 cut(s) 445
BpiI GAAGAC 2 cut(s) 109, 338
Bpu1102I GCTNAGC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 1267
BpuEI CTTGAG 2 cut(s) 148, 359
BsaBI GATNNNNATC 2 cut(s) 399, 915
BsaJI CCNNGG 2 cut(s) 47, 1015
BsaWI WCCGGW 2 cut(s) 250, 391
BsaXI ACNNNNNCTCC 4 cut(s) 1086, 1116, 1161, 1191
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 795, 796, 1064
Bse1I ACTGG 5 cut(s) 27, 451, 672, 931, 1115
Bse8I GATNNNNATC 2 cut(s) 399, 915
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 2 cut(s) 510, 1407
BseDI CCNNGG 2 cut(s) 47, 1015
BseGI GGATG 6 cut(s) 400, 445, 668, 916, 935, 1336
BseJI GATNNNNATC 2 cut(s) 399, 915
BseLI CCNNNNNNNGG 4 cut(s) 15, 795, 796, 1064
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 5 cut(s) 27, 451, 672, 931, 1115
BseSI GKGCMC 2 cut(s) 754, 1230
BseXI GCAGC 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 6 cut(s) 498, 518, 681, 752, 1114, 1189
BsiHKAI GWGCWC 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 1183
BsiSI CCGG 3 cut(s) 251, 392, 482
BslFI GGGAC 3 cut(s) 772, 887, 1410
BslI CCNNNNNNNGG 4 cut(s) 15, 795, 796, 1064
BsmFI GGGAC 3 cut(s) 772, 887, 1410
BsnI GGCC 6 cut(s) 498, 518, 681, 752, 1114, 1189
BsoBI CYCGRG 1 cut(s) 1183
Bsp119I TTCGAA 1 cut(s) 1267
Bsp120I GGGCCC 1 cut(s) 750
Bsp1286I GDGCHC 3 cut(s) 346, 754, 1230
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 7 cut(s) 357, 400, 442, 475, 712, 1054, 1333
Bsp1720I GCTNAGC 1 cut(s) 39
Bsp19I CCATGG 2 cut(s) 47, 1015
BspACI CCGC 8 cut(s) 274, 277, 285, 288, 479, 506, 519, 990
BspANI GGCC 6 cut(s) 498, 518, 681, 752, 1114, 1189
BspCNI CTCAG 1 cut(s) 31
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 7 cut(s) 477, 727, 752, 753, 988, 1056, 1198
BspMI ACCTGC 3 cut(s) 841, 1226, 1267
BspOI GCTAGC 1 cut(s) 738
BspPI GGATC 4 cut(s) 470, 483, 1049, 1062
BspT104I TTCGAA 1 cut(s) 1267
BsrI ACTGG 5 cut(s) 27, 451, 672, 931, 1115
BssECI CCNNGG 2 cut(s) 47, 1015
BssMI GATC 7 cut(s) 357, 400, 442, 475, 712, 1054, 1333
BssT1I CCWWGG 2 cut(s) 47, 1015
Bst2UI CCWGG 2 cut(s) 510, 1407
Bst4CI ACNGT 1 cut(s) 945
Bst6I CTCTTC 1 cut(s) 655
BstBI TTCGAA 1 cut(s) 1267
BstC8I GCNNGC 5 cut(s) 369, 616, 690, 736, 1150
BstDEI CTNAG 2 cut(s) 39, 644
BstDSI CCRYGG 2 cut(s) 47, 1015
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 6 cut(s) 400, 445, 668, 916, 935, 1336
BstFNI CGCG 1 cut(s) 279
BstKTI GATC 7 cut(s) 360, 403, 445, 478, 715, 1057, 1336
BstMBI GATC 7 cut(s) 357, 400, 442, 475, 712, 1054, 1333
BstMWI GCNNNNNNNGC 3 cut(s) 649, 920, 986
BstNI CCWGG 2 cut(s) 510, 1407
BstNSI RCATGY 4 cut(s) 547, 656, 692, 1276
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 2 cut(s) 508, 1405
BstSFI CTRYAG 1 cut(s) 94
BstSLI GKGCMC 2 cut(s) 754, 1230
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 65
BstV2I GAAGAC 2 cut(s) 109, 338
BstX2I RGATCY 2 cut(s) 475, 1054
BstXI CCANNNNNNTGG 1 cut(s) 1216
BstYI RGATCY 2 cut(s) 475, 1054
BsuI GTATCC 2 cut(s) 12, 1100
BsuRI GGCC 6 cut(s) 498, 518, 681, 752, 1114, 1189
BtgI CCRYGG 2 cut(s) 47, 1015
BtrI CACGTC 1 cut(s) 347
BtsCI GGATG 6 cut(s) 400, 445, 668, 916, 935, 1336
BtsI GCAGTG 1 cut(s) 1228
BtsIMutI CAGTG 1 cut(s) 1228
BveI ACCTGC 3 cut(s) 841, 1226, 1267
Cac8I GCNNGC 5 cut(s) 369, 616, 690, 736, 1150
CciI TCATGA 1 cut(s) 88
Cfr13I GGNCC 3 cut(s) 750, 751, 1403
Csp6I GTAC 1 cut(s) 1157
CviQI GTAC 1 cut(s) 1157
DdeI CTNAG 2 cut(s) 39, 644
DpnI GATC 7 cut(s) 359, 402, 444, 477, 714, 1056, 1335
DpnII GATC 7 cut(s) 357, 400, 442, 475, 712, 1054, 1333
EaeI YGGCCR 3 cut(s) 516, 679, 1112
Eam1104I CTCTTC 1 cut(s) 655
EarI CTCTTC 1 cut(s) 655
EciI GGCGGA 2 cut(s) 263, 468
Eco130I CCWWGG 2 cut(s) 47, 1015
Eco147I AGGCCT 1 cut(s) 1189
Eco24I GRGCYC 1 cut(s) 754
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 1403
Eco57I CTGAAG 1 cut(s) 1176
Eco88I CYCGRG 1 cut(s) 1183
Eco91I GGTNACC 1 cut(s) 4
EcoO109I RGGNCCY 2 cut(s) 751, 1403
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 2 cut(s) 776, 1082
EcoRII CCWGG 2 cut(s) 508, 1405
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 2 cut(s) 47, 1015
EcoT22I ATGCAT 1 cut(s) 916
EcoT38I GRGCYC 1 cut(s) 754
ErhI CCWWGG 2 cut(s) 47, 1015
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FaqI GGGAC 3 cut(s) 772, 887, 1410
FauI CCCGC 1 cut(s) 513
FbaI TGATCA 1 cut(s) 1333
Fnu4HI GCNGC 5 cut(s) 79, 277, 288, 519, 990
FokI GGATG 6 cut(s) 407, 432, 655, 923, 942, 1323
FriOI GRGCYC 1 cut(s) 754
Fsp4HI GCNGC 5 cut(s) 79, 277, 288, 519, 990
FspBI CTAG 3 cut(s) 735, 885, 1100
GluI GCNGC 5 cut(s) 79, 277, 288, 519, 990
HaeIII GGCC 6 cut(s) 498, 518, 681, 752, 1114, 1189
HapII CCGG 3 cut(s) 251, 392, 482
HindIII AAGCTT 1 cut(s) 1254
HinfI GANTC 4 cut(s) 131, 388, 888, 1095
HpaII CCGG 3 cut(s) 251, 392, 482
HphI GGTGA 4 cut(s) 419, 830, 1124, 1250
Hpy166II GTNNAC 3 cut(s) 1022, 1128, 1180
Hpy188I TCNGA 6 cut(s) 268, 297, 387, 659, 963, 1106
Hpy188III TCNNGA 7 cut(s) 89, 376, 392, 549, 860, 951, 1087
Hpy8I GTNNAC 3 cut(s) 1022, 1128, 1180
HpyAV CCTTC 7 cut(s) 236, 560, 642, 1045, 1145, 1200, 1235
HpyCH4III ACNGT 1 cut(s) 945
HpyCH4IV ACGT 3 cut(s) 299, 346, 1374
HpyCH4V TGCA 5 cut(s) 81, 692, 914, 1235, 1276
HpyF10VI GCNNNNNNNGC 3 cut(s) 649, 920, 986
HpyF3I CTNAG 2 cut(s) 39, 644
HpySE526I ACGT 3 cut(s) 299, 346, 1374
Kpn2I TCCGGA 1 cut(s) 391
Ksp22I TGATCA 1 cut(s) 1333
Kzo9I GATC 7 cut(s) 357, 400, 442, 475, 712, 1054, 1333
LmnI GCTCC 5 cut(s) 35, 540, 725, 986, 1202
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 3 cut(s) 747, 901, 920
MaeI CTAG 3 cut(s) 735, 885, 1100
MaeII ACGT 3 cut(s) 299, 346, 1374
MaeIII GTNAC 1 cut(s) 4
MalI GATC 7 cut(s) 359, 402, 444, 477, 714, 1056, 1335
MboI GATC 7 cut(s) 357, 400, 442, 475, 712, 1054, 1333
MflI RGATCY 2 cut(s) 475, 1054
MhlI GDGCHC 3 cut(s) 346, 754, 1230
MlsI TGGCCA 2 cut(s) 681, 1114
MluNI TGGCCA 2 cut(s) 681, 1114
MlyI GAGTC 2 cut(s) 397, 1104
MmeI TCCRAC 3 cut(s) 1025, 1084, 1338
MnlI CCTC 7 cut(s) 121, 589, 836, 1179, 1328, 1394, 1413
Mox20I TGGCCA 2 cut(s) 681, 1114
Mph1103I ATGCAT 1 cut(s) 916
MroI TCCGGA 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 1255
MscI TGGCCA 2 cut(s) 681, 1114
MseI TTAA 6 cut(s) 351, 455, 765, 1043, 1301, 1368
MslI CAYNNNNRTG 2 cut(s) 687, 1214
Msp20I TGGCCA 2 cut(s) 681, 1114
MspA1I CMGCKG 2 cut(s) 521, 535
MspI CCGG 3 cut(s) 251, 392, 482
MspR9I CCNGG 2 cut(s) 510, 1407
MvaI CCWGG 2 cut(s) 510, 1407
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 3 cut(s) 649, 920, 986
NcoI CCATGG 2 cut(s) 47, 1015
NdeII GATC 7 cut(s) 357, 400, 442, 475, 712, 1054, 1333
NheI GCTAGC 1 cut(s) 734
NlaIV GGNNCC 7 cut(s) 477, 727, 752, 753, 988, 1056, 1198
NmuCI GTSAC 1 cut(s) 4
NsiI ATGCAT 1 cut(s) 916
NspI RCATGY 4 cut(s) 547, 656, 692, 1276
NspV TTCGAA 1 cut(s) 1267
PaeI GCATGC 1 cut(s) 692
PaeR7I CTCGAG 1 cut(s) 1183
PagI TCATGA 1 cut(s) 88
PaqCI CACCTGC 1 cut(s) 1226
PceI AGGCCT 1 cut(s) 1189
PdmI GAANNNNTTC 1 cut(s) 1255
PfeI GAWTC 2 cut(s) 131, 888
PflMI CCANNNNNTGG 2 cut(s) 15, 1064
PfoI TCCNGGA 1 cut(s) 1405
PkrI GCNGC 5 cut(s) 80, 278, 289, 520, 991
PleI GAGTC 2 cut(s) 396, 1103
PpsI GAGTC 2 cut(s) 396, 1103
PpuMI RGGWCCY 1 cut(s) 1403
Psp1406I AACGTT 1 cut(s) 1374
Psp5II RGGWCCY 1 cut(s) 1403
Psp6I CCWGG 2 cut(s) 508, 1405
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 508, 1405
PspN4I GGNNCC 7 cut(s) 477, 727, 752, 753, 988, 1056, 1198
PspOMI GGGCCC 1 cut(s) 750
PspPI GGNCC 3 cut(s) 750, 751, 1403
PspPPI RGGWCCY 1 cut(s) 1403
PspXI VCTCGAGB 1 cut(s) 1183
PsuI RGATCY 2 cut(s) 475, 1054
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 1 cut(s) 1158
RsaNI GTAC 1 cut(s) 1157
RseI CAYNNNNRTG 2 cut(s) 687, 1214
SaqAI TTAA 6 cut(s) 351, 455, 765, 1043, 1301, 1368
SatI GCNGC 5 cut(s) 79, 277, 288, 519, 990
Sau3AI GATC 7 cut(s) 357, 400, 442, 475, 712, 1054, 1333
Sau96I GGNCC 3 cut(s) 750, 751, 1403
SchI GAGTC 2 cut(s) 397, 1104
ScrFI CCNGG 2 cut(s) 510, 1407
SduI GDGCHC 3 cut(s) 346, 754, 1230
SfaNI GCATC 3 cut(s) 747, 901, 920
SfcI CTRYAG 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 1183
SfuI TTCGAA 1 cut(s) 1267
SinI GGWCC 1 cut(s) 1403
SlaI CTCGAG 1 cut(s) 1183
SmiMI CAYNNNNRTG 2 cut(s) 687, 1214
SmlI CTYRAG 3 cut(s) 163, 374, 1183
SmoI CTYRAG 3 cut(s) 163, 374, 1183
SphI GCATGC 1 cut(s) 692
SseBI AGGCCT 1 cut(s) 1189
SsiI CCGC 8 cut(s) 274, 277, 285, 288, 479, 506, 519, 990
SspI AATATT 1 cut(s) 70
SspMI CTAG 3 cut(s) 735, 885, 1100
StuI AGGCCT 1 cut(s) 1189
StyD4I CCNGG 2 cut(s) 508, 1405
StyI CCWWGG 2 cut(s) 47, 1015
TaaI ACNGT 1 cut(s) 945
TaiI ACGT 3 cut(s) 302, 349, 1377
TaqI TCGA 3 cut(s) 441, 1184, 1267
TaqII GACCGA 1 cut(s) 505
TauI GCSGC 4 cut(s) 279, 290, 521, 992
TfiI GAWTC 2 cut(s) 131, 888
Tru1I TTAA 6 cut(s) 351, 455, 765, 1043, 1301, 1368
Tru9I TTAA 6 cut(s) 351, 455, 765, 1043, 1301, 1368
TscAI CASTG 1 cut(s) 1228
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 78
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 4 cut(s) 77, 789, 801, 1248
TspGWI ACGGA 2 cut(s) 923, 1107
TspRI CASTG 1 cut(s) 1228
Van91I CCANNNNNTGG 2 cut(s) 15, 1064
VpaK11BI GGWCC 1 cut(s) 1403
XapI RAATTY 3 cut(s) 83, 776, 1082
XceI RCATGY 4 cut(s) 547, 656, 692, 1276
XhoI CTCGAG 1 cut(s) 1183
XmnI GAANNNNTTC 1 cut(s) 1255
XspI CTAG 3 cut(s) 735, 885, 1100
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.