Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0006525

Physical Location & Seq

Genomic Coordinates Forward (+)

54613620 .. 54617250

Gene Structure 3631 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G027870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1371 bp

ATGGTCGTCATGTGTTGGAAGAGCAATAGATTGTGTTATTTTGATGACAGAAGAAGACACCAACATCAACGTAAACAACAAAGGGATTCAACAGAAGAAACAAGACAAGAAAATTTCACAACCATGGCTTCTAGCTGGTCTAATGTTTCCTCCCCAATCATTCCTCTTCTTGTTTGCTTCCTTCTTGTTTCTTCTTCAATATGTCCGGTAGTCCACTCCGCCGAAATGGCCAATCAGACTTTCAATCCGGAGCAACAGTTAAAGAAGCTGAAGAACATGAAGGCTCGTCTCAGAAAAATCAACAAGCCTGCCCTCAAGACATTTCAGAGTCCAGATGGTGATCTTATAGATTGTGTTCTTTCTCATCAGCAACCTGCTTTTGATCATCCTCGATTAAAAGGACAGAAGCCATTGGATCCACCTGAGAGACCAAAAGGTCTTAATCCCACTAGTATGATCCATGAAAATTTTCAACTATGGAGCTTGTCAGGTAATATATGCCCAGATGGTACAATTCCGATTCGAAGAACAAGAGAGCAAGACGTGCTCAGAGCTAGCTCGGTTCGAAGATACGGAAGAAAAACACCCAGGCATGTTCGAAGAGATTCATCCAGCAATGGCCATGAGCATGCAGTTGGGTATGTGAGTGGAGATCAATATTATGGAGCAAAAGCAAGTATCAATGTCTGGGCACCAAAGGTTGCGAATCAATATGAATTCAGCTTATCTCAAATGTGGGTCATTTCTGGTTCATTTGGCGACGACCTCAACACCATTGAAGCTGGTTGGCAGGTCAGCCCAGAGCTATATGGGGACAACTATCCTAGATTCTTTACTTATTGGACAACAGATGCATATCAAGCAACTGGGTGCTACAATCTGCTGTGTTCGGGTTTTGTTCAGACGAATAGTCGAATCGCCATTGGAGCTGCAATTTCTCCAACCTCTGGTTACAATGGTGGACAGTTTGATATTAGTTTATTGATTTGGAAGGATCCAAAGCATGGAAATTGGTGGCTGGAATTTGGGAATGGGGTTCTAGTTGGATACTGGCCATCATTCTTGTTCACTCATCTTCAAGACCACGCAAGCATGGTACAATTTGGTGGAGAAGTTGTAAACTCAAGGCCTTCTGGATCTCACACTGCAACACAAATGGGAAGTGGTCATTTTGCAGGAGAAGGTTTTGGCAAAGCTTCTTACTTTAGAAACTTGCAGGTTGTGGATTGGGACAACAGCCTAATCCCATTATCAAACCTCAAAGTTTTGGCAGATCATCCAAATTGCTATGACATCCAAGGAGGCATTAACAATTTTTGGGGGAATTACTTTTACTATGGTGGTCCTGGAAGAAATGTGAGGTGTCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Neprosin Neprosin_AP

Protein Analysis

456

Amino Acids

51.54

Weight (kDa)

8.89

Isoelectric Point (pI)

35.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Neprosin_AP	PF14365	107 - 214	1.3e-36	Neprosin activation peptide
Neprosin	PF03080	238 - 449	4.5e-87	Neprosin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006525)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10750 AT1G10750 AT1G23340 AT1G23340 AT1G23340 AT1G70550 AT1G70550
fragaria_vesca	FvH4_4g25820 FvH4_4g25820 FvH4_4g25820 FvH4_4g25820
malus_domestica	MD13G1097000.v1.1 MD16G1097900.v1.1
prunus_persica	Prupe.1G248600_v2.0.a1
pyrus_communis	pycom13g08470 pycom16g08340
rosa_chinensis	RchiOBHm_Chr4g0433271
rosa_laevigata	RLG00000006768
rosa_multiflora	Rmu_sc0022950.1_g000002
rosa_roxburghii	Rroxscaffold_5G00374830
rosa_rugosa	Rorug04G0267600
rosa_samantha	Rh4AG322100 Rh4BG329500 Rh4CG344800 Rh4DG326200
rosa_wichuraiana	Rw4G027870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	435
Acc36I	ACCTGC	3 cut(s)	382, 781, 1207
AccB1I	GGYRCC	1 cut(s)	691
AccB7I	CCANNNNNTGG	2 cut(s)	947, 1004
AccIII	TCCGGA	1 cut(s)	247
AciI	CCGC	1 cut(s)	219
AclWI	GGATC	6 cut(s)	410, 423, 451, 989, 1002, 1144
AcoI	YGGCCR	3 cut(s)	228, 619, 1052
AcsI	RAATTY	4 cut(s)	112, 466, 716, 1022
AcuI	CTGAAG	1 cut(s)	290
AfaI	GTAC	2 cut(s)	511, 1098
AfiI	CCNNNNNNNGG	2 cut(s)	947, 1004
AgsI	TTSAA	6 cut(s)	90, 198, 244, 473, 779, 1079
AhdI	GACNNNNNGTC	1 cut(s)	909
AhlI	ACTAGT	1 cut(s)	449
AjiI	CACGTC	1 cut(s)	544
AjnI	CCWGG	2 cut(s)	587, 1345
AjuI	GAANNNNNNNTTGG	2 cut(s)	224, 256
Alw21I	GWGCWC	1 cut(s)	549
Alw26I	GTCTC	2 cut(s)	293, 421
AlwI	GGATC	6 cut(s)	410, 423, 451, 989, 1002, 1144
Aor13HI	TCCGGA	1 cut(s)	247
AoxI	GGCC	4 cut(s)	228, 619, 1052, 1127
ApeKI	GCWGC	1 cut(s)	929
ApoI	RAATTY	4 cut(s)	112, 466, 716, 1022
Asp700I	GAANNNNTTC	2 cut(s)	468, 604
AspS9I	GGNCC	1 cut(s)	1343
AsuHPI	GGTGA	1 cut(s)	350
AsuII	TTCGAA	3 cut(s)	523, 565, 598
AsuNHI	GCTAGC	1 cut(s)	554
AvaII	GGWCC	1 cut(s)	1343
BaeGI	GKGCMC	1 cut(s)	694
BalI	TGGCCA	3 cut(s)	230, 621, 1054
BamHI	GGATCC	2 cut(s)	415, 994
BanI	GGYRCC	1 cut(s)	691
BbsI	GAAGAC	1 cut(s)	61
Bbv12I	GWGCWC	1 cut(s)	549
BbvI	GCAGC	1 cut(s)	916
BccI	CCATC	3 cut(s)	329, 500, 1063
BciT130I	CCWGG	2 cut(s)	589, 1347
BciVI	GTATCC	1 cut(s)	1040
BclI	TGATCA	1 cut(s)	382
BcoDI	GTCTC	2 cut(s)	293, 421
BcuI	ACTAGT	1 cut(s)	449
BfaI	CTAG	5 cut(s)	132, 450, 555, 825, 1040
BfuAI	ACCTGC	3 cut(s)	382, 781, 1207
BfuI	GTATCC	1 cut(s)	1040
BglI	GCCNNNNNGGC	1 cut(s)	227
BisI	GCNGC	1 cut(s)	930
BlsI	GCNGC	1 cut(s)	931
Bme1390I	CCNGG	2 cut(s)	589, 1347
Bme18I	GGWCC	1 cut(s)	1343
BmeRI	GACNNNNNGTC	1 cut(s)	909
BmgBI	CACGTC	1 cut(s)	544
BmgT120I	GGNCC	1 cut(s)	1343
BmiI	GGNNCC	3 cut(s)	417, 693, 996
BmrFI	CCNGG	2 cut(s)	589, 1347
BmrI	ACTGGG	1 cut(s)	876
BmsI	GCATC	1 cut(s)	841
BmtI	GCTAGC	1 cut(s)	558
BmuI	ACTGGG	1 cut(s)	876
BpiI	GAAGAC	1 cut(s)	61
Bpu14I	TTCGAA	3 cut(s)	523, 565, 598
BpuEI	CTTGAG	2 cut(s)	299, 1108
BsaBI	GATNNNNATC	2 cut(s)	339, 855
BsaI	GGTCTC	1 cut(s)	421
BsaJI	CCNNGG	3 cut(s)	123, 587, 1297
BsaWI	WCCGGW	2 cut(s)	205, 247
BsaXI	ACNNNNNCTCC	4 cut(s)	242, 272, 1101, 1131
Bsc4I	CCNNNNNNNGG	2 cut(s)	947, 1004
Bse1I	ACTGG	2 cut(s)	871, 1055
Bse3DI	GCAATG	1 cut(s)	622
Bse8I	GATNNNNATC	2 cut(s)	339, 855
BseAI	TCCGGA	1 cut(s)	247
BseBI	CCWGG	2 cut(s)	589, 1347
BseDI	CCNNGG	3 cut(s)	123, 587, 1297
BseGI	GGATG	4 cut(s)	385, 608, 1276, 1293
BseJI	GATNNNNATC	2 cut(s)	339, 855
BseLI	CCNNNNNNNGG	2 cut(s)	947, 1004
BseMI	GCAATG	1 cut(s)	622
BseMII	CTCAG	3 cut(s)	304, 414, 562
BseNI	ACTGG	2 cut(s)	871, 1055
BseSI	GKGCMC	1 cut(s)	694
BseXI	GCAGC	1 cut(s)	916
BshFI	GGCC	4 cut(s)	230, 621, 1054, 1129
BshNI	GGYRCC	1 cut(s)	691
BsiHKAI	GWGCWC	1 cut(s)	549
BsiSI	CCGG	2 cut(s)	206, 248
BslFI	GGGAC	3 cut(s)	827, 1244, 1350
BslI	CCNNNNNNNGG	2 cut(s)	947, 1004
BsmAI	GTCTC	2 cut(s)	293, 421
BsmBI	CGTCTC	1 cut(s)	293
BsmFI	GGGAC	3 cut(s)	827, 1244, 1350
BsnI	GGCC	4 cut(s)	230, 621, 1054, 1129
Bso31I	GGTCTC	1 cut(s)	421
Bsp119I	TTCGAA	3 cut(s)	523, 565, 598
Bsp1286I	GDGCHC	2 cut(s)	549, 694
Bsp13I	TCCGGA	1 cut(s)	247
Bsp143I	GATC	8 cut(s)	340, 382, 415, 456, 652, 994, 1136, 1273
Bsp19I	CCATGG	1 cut(s)	123
BspACI	CCGC	1 cut(s)	219
BspANI	GGCC	4 cut(s)	230, 621, 1054, 1129
BspCNI	CTCAG	3 cut(s)	303, 415, 561
BspEI	TCCGGA	1 cut(s)	247
BspLI	GGNNCC	3 cut(s)	417, 693, 996
BspMI	ACCTGC	3 cut(s)	382, 781, 1207
BspOI	GCTAGC	1 cut(s)	558
BspPI	GGATC	6 cut(s)	410, 423, 451, 989, 1002, 1144
BspQI	GCTCTTC	1 cut(s)	14
BspT104I	TTCGAA	3 cut(s)	523, 565, 598
BspT107I	GGYRCC	1 cut(s)	691
BspTNI	GGTCTC	1 cut(s)	421
BsrDI	GCAATG	1 cut(s)	622
BsrI	ACTGG	2 cut(s)	871, 1055
BssECI	CCNNGG	3 cut(s)	123, 587, 1297
BssMI	GATC	8 cut(s)	340, 382, 415, 456, 652, 994, 1136, 1273
BssT1I	CCWWGG	2 cut(s)	123, 1297
Bst2UI	CCWGG	2 cut(s)	589, 1347
Bst4CI	ACNGT	2 cut(s)	258, 966
Bst6I	CTCTTC	3 cut(s)	14, 171, 595
BstAPI	GCANNNNNTGC	1 cut(s)	544
BstBI	TTCGAA	3 cut(s)	523, 565, 598
BstC8I	GCNNGC	4 cut(s)	309, 556, 630, 1090
BstDEI	CTNAG	3 cut(s)	290, 423, 548
BstDSI	CCRYGG	1 cut(s)	123
BstF5I	GGATG	4 cut(s)	385, 608, 1276, 1293
BstKTI	GATC	8 cut(s)	343, 385, 418, 459, 655, 997, 1139, 1276
BstMAI	GTCTC	2 cut(s)	293, 421
BstMBI	GATC	8 cut(s)	340, 382, 415, 456, 652, 994, 1136, 1273
BstMWI	GCNNNNNNNGC	4 cut(s)	227, 544, 860, 926
BstNI	CCWGG	2 cut(s)	589, 1347
BstNSI	RCATGY	2 cut(s)	596, 632
BstSCI	CCNGG	2 cut(s)	587, 1345
BstSLI	GKGCMC	1 cut(s)	694
BstV1I	GCAGC	1 cut(s)	916
BstV2I	GAAGAC	1 cut(s)	61
BstX2I	RGATCY	3 cut(s)	415, 994, 1136
BstYI	RGATCY	3 cut(s)	415, 994, 1136
BsuI	GTATCC	1 cut(s)	1040
BsuRI	GGCC	4 cut(s)	230, 621, 1054, 1129
BtgI	CCRYGG	1 cut(s)	123
BtrI	CACGTC	1 cut(s)	544
BtsCI	GGATG	4 cut(s)	385, 608, 1276, 1293
BtsI	GCAGTG	1 cut(s)	1143
BtsIMutI	CAGTG	1 cut(s)	1143
BveI	ACCTGC	3 cut(s)	382, 781, 1207
Cac8I	GCNNGC	4 cut(s)	309, 556, 630, 1090
Cfr13I	GGNCC	1 cut(s)	1343
Csp6I	GTAC	2 cut(s)	510, 1097
CviAII	CATG	9 cut(s)	10, 124, 277, 461, 593, 623, 629, 1004, 1093
CviQI	GTAC	2 cut(s)	510, 1097
DdeI	CTNAG	3 cut(s)	290, 423, 548
DpnI	GATC	8 cut(s)	342, 384, 417, 458, 654, 996, 1138, 1275
DpnII	GATC	8 cut(s)	340, 382, 415, 456, 652, 994, 1136, 1273
DrdI	GACNNNNNNGTC	1 cut(s)	435
DriI	GACNNNNNGTC	1 cut(s)	909
DseDI	GACNNNNNNGTC	1 cut(s)	435
EaeI	YGGCCR	3 cut(s)	228, 619, 1052
Eam1104I	CTCTTC	3 cut(s)	14, 171, 595
Eam1105I	GACNNNNNGTC	1 cut(s)	909
EarI	CTCTTC	3 cut(s)	14, 171, 595
EciI	GGCGGA	1 cut(s)	208
Eco130I	CCWWGG	2 cut(s)	123, 1297
Eco147I	AGGCCT	1 cut(s)	1129
Eco31I	GGTCTC	1 cut(s)	421
Eco47I	GGWCC	1 cut(s)	1343
Eco57I	CTGAAG	1 cut(s)	290
EcoRI	GAATTC	1 cut(s)	716
EcoRII	CCWGG	2 cut(s)	587, 1345
EcoT14I	CCWWGG	2 cut(s)	123, 1297
EcoT22I	ATGCAT	1 cut(s)	856
ErhI	CCWWGG	2 cut(s)	123, 1297
Esp3I	CGTCTC	1 cut(s)	293
FaeI	CATG	9 cut(s)	13, 127, 280, 464, 596, 626, 632, 1007, 1096
FaqI	GGGAC	3 cut(s)	827, 1244, 1350
FatI	CATG	9 cut(s)	9, 123, 276, 460, 592, 622, 628, 1003, 1092
FbaI	TGATCA	1 cut(s)	382
Fnu4HI	GCNGC	1 cut(s)	930
FokI	GGATG	4 cut(s)	372, 595, 1263, 1280
Fsp4HI	GCNGC	1 cut(s)	930
FspBI	CTAG	5 cut(s)	132, 450, 555, 825, 1040
GluI	GCNGC	1 cut(s)	930
HaeIII	GGCC	4 cut(s)	230, 621, 1054, 1129
HapII	CCGG	2 cut(s)	206, 248
Hin1II	CATG	9 cut(s)	13, 127, 280, 464, 596, 626, 632, 1007, 1096
HindIII	AAGCTT	1 cut(s)	1194
HinfI	GANTC	7 cut(s)	86, 328, 520, 605, 706, 828, 915
HpaII	CCGG	2 cut(s)	206, 248
HphI	GGTGA	1 cut(s)	350
Hpy166II	GTNNAC	5 cut(s)	74, 214, 962, 1068, 1120
Hpy188I	TCNGA	6 cut(s)	237, 293, 327, 519, 551, 903
Hpy188III	TCNNGA	5 cut(s)	248, 316, 332, 1079, 1134
Hpy8I	GTNNAC	5 cut(s)	74, 214, 962, 1068, 1120
Hpy99I	CGWCG	1 cut(s)	764
HpyAV	CCTTC	5 cut(s)	191, 274, 985, 1140, 1175
HpyCH4III	ACNGT	2 cut(s)	258, 966
HpyCH4IV	ACGT	2 cut(s)	70, 543
HpyCH4V	TGCA	6 cut(s)	632, 854, 932, 1148, 1175, 1216
HpyF10VI	GCNNNNNNNGC	4 cut(s)	227, 544, 860, 926
HpyF3I	CTNAG	3 cut(s)	290, 423, 548
HpySE526I	ACGT	2 cut(s)	70, 543
Hsp92II	CATG	9 cut(s)	13, 127, 280, 464, 596, 626, 632, 1007, 1096
Kpn2I	TCCGGA	1 cut(s)	247
Ksp22I	TGATCA	1 cut(s)	382
Kzo9I	GATC	8 cut(s)	340, 382, 415, 456, 652, 994, 1136, 1273
LguI	GCTCTTC	1 cut(s)	14
LmnI	GCTCC	4 cut(s)	250, 480, 665, 926
Lsp1109I	GCAGC	1 cut(s)	916
LweI	GCATC	1 cut(s)	841
MaeI	CTAG	5 cut(s)	132, 450, 555, 825, 1040
MaeII	ACGT	2 cut(s)	70, 543
MaeIII	GTNAC	1 cut(s)	950
MalI	GATC	8 cut(s)	342, 384, 417, 458, 654, 996, 1138, 1275
MboI	GATC	8 cut(s)	340, 382, 415, 456, 652, 994, 1136, 1273
MflI	RGATCY	3 cut(s)	415, 994, 1136
MhlI	GDGCHC	2 cut(s)	549, 694
MlsI	TGGCCA	3 cut(s)	230, 621, 1054
MluNI	TGGCCA	3 cut(s)	230, 621, 1054
MlyI	GAGTC	1 cut(s)	337
MmeI	TCCRAC	2 cut(s)	965, 1024
MnlI	CCTC	9 cut(s)	160, 174, 323, 399, 776, 955, 1268, 1295, 1353
Mox20I	TGGCCA	3 cut(s)	230, 621, 1054
Mph1103I	ATGCAT	1 cut(s)	856
MroI	TCCGGA	1 cut(s)	247
MroXI	GAANNNNTTC	2 cut(s)	468, 604
MscI	TGGCCA	3 cut(s)	230, 621, 1054
MseI	TTAA	4 cut(s)	260, 395, 441, 1308
MslI	CAYNNNNRTG	3 cut(s)	122, 452, 627
Msp20I	TGGCCA	3 cut(s)	230, 621, 1054
MspI	CCGG	2 cut(s)	206, 248
MspR9I	CCNGG	2 cut(s)	589, 1347
MvaI	CCWGG	2 cut(s)	589, 1347
MwoI	GCNNNNNNNGC	4 cut(s)	227, 544, 860, 926
NcoI	CCATGG	1 cut(s)	123
NdeII	GATC	8 cut(s)	340, 382, 415, 456, 652, 994, 1136, 1273
NheI	GCTAGC	1 cut(s)	554
NlaIII	CATG	9 cut(s)	13, 127, 280, 464, 596, 626, 632, 1007, 1096
NlaIV	GGNNCC	3 cut(s)	417, 693, 996
NsiI	ATGCAT	1 cut(s)	856
NspI	RCATGY	2 cut(s)	596, 632
NspV	TTCGAA	3 cut(s)	523, 565, 598
PaeI	GCATGC	1 cut(s)	632
PceI	AGGCCT	1 cut(s)	1129
PciSI	GCTCTTC	1 cut(s)	14
PdmI	GAANNNNTTC	2 cut(s)	468, 604
PfeI	GAWTC	6 cut(s)	86, 520, 605, 706, 828, 915
PflMI	CCANNNNNTGG	2 cut(s)	947, 1004
PfoI	TCCNGGA	1 cut(s)	1345
PkrI	GCNGC	1 cut(s)	931
PleI	GAGTC	1 cut(s)	336
PpsI	GAGTC	1 cut(s)	336
Psp6I	CCWGG	2 cut(s)	587, 1345
PspGI	CCWGG	2 cut(s)	587, 1345
PspN4I	GGNNCC	3 cut(s)	417, 693, 996
PspPI	GGNCC	1 cut(s)	1343
PsuI	RGATCY	3 cut(s)	415, 994, 1136
RsaI	GTAC	2 cut(s)	511, 1098
RsaNI	GTAC	2 cut(s)	510, 1097
RseI	CAYNNNNRTG	3 cut(s)	122, 452, 627
SapI	GCTCTTC	1 cut(s)	14
SaqAI	TTAA	4 cut(s)	260, 395, 441, 1308
SatI	GCNGC	1 cut(s)	930
Sau3AI	GATC	8 cut(s)	340, 382, 415, 456, 652, 994, 1136, 1273
Sau96I	GGNCC	1 cut(s)	1343
SchI	GAGTC	1 cut(s)	337
ScrFI	CCNGG	2 cut(s)	589, 1347
SduI	GDGCHC	2 cut(s)	549, 694
SfaNI	GCATC	1 cut(s)	841
SfuI	TTCGAA	3 cut(s)	523, 565, 598
SinI	GGWCC	1 cut(s)	1343
SmiMI	CAYNNNNRTG	3 cut(s)	122, 452, 627
SmlI	CTYRAG	2 cut(s)	314, 1123
SmoI	CTYRAG	2 cut(s)	314, 1123
SpeI	ACTAGT	1 cut(s)	449
SphI	GCATGC	1 cut(s)	632
SseBI	AGGCCT	1 cut(s)	1129
SsiI	CCGC	1 cut(s)	219
SspI	AATATT	1 cut(s)	659
SspMI	CTAG	5 cut(s)	132, 450, 555, 825, 1040
StuI	AGGCCT	1 cut(s)	1129
StyD4I	CCNGG	2 cut(s)	587, 1345
StyI	CCWWGG	2 cut(s)	123, 1297
TaaI	ACNGT	2 cut(s)	258, 966
TaiI	ACGT	2 cut(s)	73, 546
TaqI	TCGA	5 cut(s)	391, 523, 565, 598, 913
TfiI	GAWTC	6 cut(s)	86, 520, 605, 706, 828, 915
Tru1I	TTAA	4 cut(s)	260, 395, 441, 1308
Tru9I	TTAA	4 cut(s)	260, 395, 441, 1308
TscAI	CASTG	1 cut(s)	1150
TseI	GCWGC	1 cut(s)	929
TspDTI	ATGAA	5 cut(s)	293, 477, 597, 729, 741
TspGWI	ACGGA	1 cut(s)	588
TspRI	CASTG	1 cut(s)	1150
Van91I	CCANNNNNTGG	2 cut(s)	947, 1004
VpaK11BI	GGWCC	1 cut(s)	1343
XapI	RAATTY	4 cut(s)	112, 466, 716, 1022
XceI	RCATGY	2 cut(s)	596, 632
XmnI	GAANNNNTTC	2 cut(s)	468, 604
XspI	CTAG	5 cut(s)	132, 450, 555, 825, 1040
Zsp2I	ATGCAT	1 cut(s)	856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.