Rh4CG344800

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006525
Physical Location & Seq
Forward (+)
60425958 .. 60429976
4019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG344800.1

Sequence Viewer

Length: 1005 bp
ATGGTCGTCATGTGTTGGAAGAGCAATAGATTGTGTTATTTTGATGACAGAAGAAGACACCAACATCAACGTAAACAACAAAGGGATTCAACAGAAGAAACAAGACAAGAAAATTTCACAGCCATGGCTTCTAGCTGGTCTAATGTTTCCTCCCCAATCATTCCTCTTCTTGTTTGCTTCCTTCTTGTTTCTTCTTCAATATGTCCGGTAGTCCACGCCGCCGAAATGGCCAATCAGACTTTCAATCCGGAGCAACAGTTAAAGAAGCTGAAGAACATGAAGGCTCGTCTCAGAAAAATCAACAAGCCTGCCCTCAAGACATTTCAGAGTCCAGATGGTGATCTTATAGATTGTGTTCTTTCTCATCAGCAACCTGCTTTTGATCATCCTCGATTAAAAGGACAGAAGCCATTGGATCCACCTGAGAGACCAAAAGGTCTTAATCCGACTAGTATGATCCATGAAAATTTTCAACTATGGAGCTTGTCAGGTAATATATGCCCAGATGGTACAATTCCGATTCGAAGAACAAGAGAGCAAGACGTGCTCAGAGCTAGCTCGGTTCGAAGATACGGAAGAAAAACACCCAGGCATGTTCGAAGAGATTCATCCAGCAATGGCCATGAGCATGCAGTTGGGTATGTGAGTGGAGATCAATACTATGGAGCAAAAGCAAGTATCAATGTCTGGGCACCAAAGGTTGCAAATCAATATGAATTCAGCTTATCTCAAATGTGGGTCATTTCTGGTTCATTTGGCGACGACCTCAACACCATTGAAGCTGGTTGGCAGGTCAGCCCGGAGCTATATGGGGACAACTATCCTAGATTCTTTACTTACTGGACTACAGATGCATATCAAGCAACTGGGTGCTACAATCTGCTGTGTTCGGGTTTTGTTCAGACGAATAGTCGAATCGCCATTGGAGCTGCAATTTCTCCAACCTCTGGTTACAATGGTGGACAGTTTGATATTAGTTTATTGATTTGGAAGGTAAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

334

Amino Acids

37.91

Weight (kDa)

9.22

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 107 - 214 7e-37 Neprosin activation peptide
Neprosin PF03080 238 - 331 2.1e-38 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 435
Acc36I ACCTGC 2 cut(s) 382, 781
AccB1I GGYRCC 1 cut(s) 691
AccB7I CCANNNNNTGG 1 cut(s) 947
AccIII TCCGGA 1 cut(s) 247
AciI CCGC 1 cut(s) 219
AclWI GGATC 3 cut(s) 410, 423, 451
AcoI YGGCCR 2 cut(s) 228, 619
AcsI RAATTY 3 cut(s) 112, 466, 716
AcuI CTGAAG 1 cut(s) 290
AfaI GTAC 1 cut(s) 511
AfiI CCNNNNNNNGG 1 cut(s) 947
AgsI TTSAA 5 cut(s) 90, 198, 244, 473, 779
AhdI GACNNNNNGTC 1 cut(s) 909
AhlI ACTAGT 1 cut(s) 449
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 1 cut(s) 587
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 9 cut(s) 135, 268, 483, 554, 558, 723, 782, 805, 929
AluI AGCT 9 cut(s) 135, 268, 483, 554, 558, 723, 782, 805, 929
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 293, 421
AlwI GGATC 3 cut(s) 410, 423, 451
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 2 cut(s) 228, 619
ApeKI GCWGC 1 cut(s) 929
ApoI RAATTY 3 cut(s) 112, 466, 716
Asp700I GAANNNNTTC 2 cut(s) 468, 604
AsuC2I CCSGG 1 cut(s) 800
AsuHPI GGTGA 1 cut(s) 350
AsuII TTCGAA 3 cut(s) 523, 565, 598
AsuNHI GCTAGC 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 694
BalI TGGCCA 2 cut(s) 230, 621
BamHI GGATCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 691
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 916
BccI CCATC 2 cut(s) 329, 500
BciT130I CCWGG 1 cut(s) 589
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 800
BcoDI GTCTC 2 cut(s) 293, 421
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 5 cut(s) 132, 450, 555, 825, 1003
BfmI CTRYAG 1 cut(s) 846
BfuAI ACCTGC 2 cut(s) 382, 781
BglI GCCNNNNNGGC 1 cut(s) 227
BisI GCNGC 2 cut(s) 219, 930
BlsI GCNGC 2 cut(s) 220, 931
Bme1390I CCNGG 2 cut(s) 589, 800
BmeRI GACNNNNNGTC 1 cut(s) 909
BmgBI CACGTC 1 cut(s) 544
BmiI GGNNCC 2 cut(s) 417, 693
BmrFI CCNGG 2 cut(s) 589, 800
BmrI ACTGGG 1 cut(s) 876
BmsI GCATC 1 cut(s) 841
BmtI GCTAGC 1 cut(s) 558
BmuI ACTGGG 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 61
Bpu14I TTCGAA 3 cut(s) 523, 565, 598
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 800
BsaBI GATNNNNATC 2 cut(s) 339, 855
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 2 cut(s) 123, 587
BsaWI WCCGGW 2 cut(s) 205, 247
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 947
Bse1I ACTGG 2 cut(s) 845, 871
Bse3DI GCAATG 1 cut(s) 622
Bse8I GATNNNNATC 2 cut(s) 339, 855
BseAI TCCGGA 1 cut(s) 247
BseBI CCWGG 1 cut(s) 589
BseDI CCNNGG 2 cut(s) 123, 587
BseGI GGATG 2 cut(s) 385, 608
BseJI GATNNNNATC 2 cut(s) 339, 855
BseLI CCNNNNNNNGG 1 cut(s) 947
BseMI GCAATG 1 cut(s) 622
BseMII CTCAG 3 cut(s) 304, 414, 562
BseNI ACTGG 2 cut(s) 845, 871
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 1 cut(s) 916
BshFI GGCC 2 cut(s) 230, 621
BshNI GGYRCC 1 cut(s) 691
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 3 cut(s) 206, 248, 800
BslFI GGGAC 1 cut(s) 827
BslI CCNNNNNNNGG 1 cut(s) 947
BsmAI GTCTC 2 cut(s) 293, 421
BsmBI CGTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 827
BsnI GGCC 2 cut(s) 230, 621
Bso31I GGTCTC 1 cut(s) 421
Bsp119I TTCGAA 3 cut(s) 523, 565, 598
Bsp1286I GDGCHC 2 cut(s) 549, 694
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 5 cut(s) 340, 382, 415, 456, 652
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 1 cut(s) 219
BspANI GGCC 2 cut(s) 230, 621
BspCNI CTCAG 3 cut(s) 303, 415, 561
BspEI TCCGGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 417, 693
BspMI ACCTGC 2 cut(s) 382, 781
BspOI GCTAGC 1 cut(s) 558
BspPI GGATC 3 cut(s) 410, 423, 451
BspQI GCTCTTC 1 cut(s) 14
BspT104I TTCGAA 3 cut(s) 523, 565, 598
BspT107I GGYRCC 1 cut(s) 691
BspTNI GGTCTC 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 622
BsrI ACTGG 2 cut(s) 845, 871
BssECI CCNNGG 2 cut(s) 123, 587
BssMI GATC 5 cut(s) 340, 382, 415, 456, 652
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 589
Bst4CI ACNGT 2 cut(s) 258, 966
Bst6I CTCTTC 3 cut(s) 14, 171, 595
BstAPI GCANNNNNTGC 1 cut(s) 544
BstBI TTCGAA 3 cut(s) 523, 565, 598
BstC8I GCNNGC 3 cut(s) 309, 556, 630
BstDEI CTNAG 3 cut(s) 290, 423, 548
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 2 cut(s) 385, 608
BstKTI GATC 5 cut(s) 343, 385, 418, 459, 655
BstMAI GTCTC 2 cut(s) 293, 421
BstMBI GATC 5 cut(s) 340, 382, 415, 456, 652
BstMWI GCNNNNNNNGC 4 cut(s) 227, 544, 860, 926
BstNI CCWGG 1 cut(s) 589
BstNSI RCATGY 2 cut(s) 596, 632
BstSCI CCNGG 2 cut(s) 587, 798
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 1 cut(s) 694
BstV1I GCAGC 1 cut(s) 916
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 2 cut(s) 230, 621
BtgI CCRYGG 1 cut(s) 123
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 2 cut(s) 385, 608
BveI ACCTGC 2 cut(s) 382, 781
Cac8I GCNNGC 3 cut(s) 309, 556, 630
Csp6I GTAC 1 cut(s) 510
CviAII CATG 7 cut(s) 10, 124, 277, 461, 593, 623, 629
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 3 cut(s) 290, 423, 548
DpnI GATC 5 cut(s) 342, 384, 417, 458, 654
DpnII GATC 5 cut(s) 340, 382, 415, 456, 652
DrdI GACNNNNNNGTC 1 cut(s) 435
DriI GACNNNNNGTC 1 cut(s) 909
DseDI GACNNNNNNGTC 1 cut(s) 435
EaeI YGGCCR 2 cut(s) 228, 619
Eam1104I CTCTTC 3 cut(s) 14, 171, 595
Eam1105I GACNNNNNGTC 1 cut(s) 909
EarI CTCTTC 3 cut(s) 14, 171, 595
Eco130I CCWWGG 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 716
EcoRII CCWGG 1 cut(s) 587
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 1 cut(s) 856
ErhI CCWWGG 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 7 cut(s) 13, 127, 280, 464, 596, 626, 632
FaqI GGGAC 1 cut(s) 827
FatI CATG 7 cut(s) 9, 123, 276, 460, 592, 622, 628
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 219, 930
FokI GGATG 2 cut(s) 372, 595
Fsp4HI GCNGC 2 cut(s) 219, 930
FspBI CTAG 5 cut(s) 132, 450, 555, 825, 1003
GluI GCNGC 2 cut(s) 219, 930
HaeIII GGCC 2 cut(s) 230, 621
HapII CCGG 3 cut(s) 206, 248, 800
Hin1II CATG 7 cut(s) 13, 127, 280, 464, 596, 626, 632
HinfI GANTC 6 cut(s) 86, 328, 520, 605, 828, 915
HpaII CCGG 3 cut(s) 206, 248, 800
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 3 cut(s) 74, 214, 962
Hpy188I TCNGA 7 cut(s) 237, 293, 327, 447, 519, 551, 903
Hpy188III TCNNGA 3 cut(s) 248, 316, 332
Hpy8I GTNNAC 3 cut(s) 74, 214, 962
Hpy99I CGWCG 1 cut(s) 764
HpyAV CCTTC 3 cut(s) 191, 274, 985
HpyCH4III ACNGT 2 cut(s) 258, 966
HpyCH4IV ACGT 2 cut(s) 70, 543
HpyCH4V TGCA 4 cut(s) 632, 704, 854, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 544, 860, 926
HpyF3I CTNAG 3 cut(s) 290, 423, 548
HpySE526I ACGT 2 cut(s) 70, 543
Hsp92II CATG 7 cut(s) 13, 127, 280, 464, 596, 626, 632
Kpn2I TCCGGA 1 cut(s) 247
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 340, 382, 415, 456, 652
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 5 cut(s) 250, 480, 665, 802, 926
Lsp1109I GCAGC 1 cut(s) 916
LweI GCATC 1 cut(s) 841
MaeI CTAG 5 cut(s) 132, 450, 555, 825, 1003
MaeII ACGT 2 cut(s) 70, 543
MaeIII GTNAC 1 cut(s) 950
MalI GATC 5 cut(s) 342, 384, 417, 458, 654
MboI GATC 5 cut(s) 340, 382, 415, 456, 652
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 2 cut(s) 549, 694
MlsI TGGCCA 2 cut(s) 230, 621
MluCI AATT 5 cut(s) 112, 466, 513, 716, 933
MluNI TGGCCA 2 cut(s) 230, 621
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 2 cut(s) 470, 965
MnlI CCTC 6 cut(s) 160, 174, 323, 399, 776, 955
Mox20I TGGCCA 2 cut(s) 230, 621
Mph1103I ATGCAT 1 cut(s) 856
MroI TCCGGA 1 cut(s) 247
MroXI GAANNNNTTC 2 cut(s) 468, 604
MscI TGGCCA 2 cut(s) 230, 621
MseI TTAA 3 cut(s) 260, 395, 441
MslI CAYNNNNRTG 2 cut(s) 122, 627
Msp20I TGGCCA 2 cut(s) 230, 621
MspI CCGG 3 cut(s) 206, 248, 800
MspR9I CCNGG 2 cut(s) 589, 800
MvaI CCWGG 1 cut(s) 589
MwoI GCNNNNNNNGC 4 cut(s) 227, 544, 860, 926
NciI CCSGG 1 cut(s) 800
NcoI CCATGG 1 cut(s) 123
NdeII GATC 5 cut(s) 340, 382, 415, 456, 652
NheI GCTAGC 1 cut(s) 554
NlaIII CATG 7 cut(s) 13, 127, 280, 464, 596, 626, 632
NlaIV GGNNCC 2 cut(s) 417, 693
NsiI ATGCAT 1 cut(s) 856
NspI RCATGY 2 cut(s) 596, 632
NspV TTCGAA 3 cut(s) 523, 565, 598
PaeI GCATGC 1 cut(s) 632
PciSI GCTCTTC 1 cut(s) 14
PdmI GAANNNNTTC 2 cut(s) 468, 604
PfeI GAWTC 5 cut(s) 86, 520, 605, 828, 915
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 2 cut(s) 220, 931
PleI GAGTC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 587
PspGI CCWGG 1 cut(s) 587
PspN4I GGNNCC 2 cut(s) 417, 693
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
RseI CAYNNNNRTG 2 cut(s) 122, 627
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 3 cut(s) 260, 395, 441
SatI GCNGC 2 cut(s) 219, 930
Sau3AI GATC 5 cut(s) 340, 382, 415, 456, 652
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 2 cut(s) 589, 800
SduI GDGCHC 2 cut(s) 549, 694
SfaNI GCATC 1 cut(s) 841
SfcI CTRYAG 1 cut(s) 846
SfuI TTCGAA 3 cut(s) 523, 565, 598
SmiMI CAYNNNNRTG 2 cut(s) 122, 627
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
SpeI ACTAGT 1 cut(s) 449
SphI GCATGC 1 cut(s) 632
Sse9I AATT 5 cut(s) 112, 466, 513, 716, 933
SsiI CCGC 1 cut(s) 219
SspMI CTAG 5 cut(s) 132, 450, 555, 825, 1003
StyD4I CCNGG 2 cut(s) 587, 798
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 258, 966
TaiI ACGT 2 cut(s) 73, 546
TaqI TCGA 5 cut(s) 391, 523, 565, 598, 913
TasI AATT 5 cut(s) 112, 466, 513, 716, 933
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 5 cut(s) 86, 520, 605, 828, 915
Tru1I TTAA 3 cut(s) 260, 395, 441
Tru9I TTAA 3 cut(s) 260, 395, 441
TseI GCWGC 1 cut(s) 929
TspDTI ATGAA 5 cut(s) 293, 477, 597, 729, 741
TspGWI ACGGA 1 cut(s) 588
Van91I CCANNNNNTGG 1 cut(s) 947
XapI RAATTY 3 cut(s) 112, 466, 716
XceI RCATGY 2 cut(s) 596, 632
XmnI GAANNNNTTC 2 cut(s) 468, 604
XspI CTAG 5 cut(s) 132, 450, 555, 825, 1003
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.