Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006525

Physical Location & Seq

Genomic Coordinates Forward (+)

6871524 .. 6877575

Gene Structure 6052 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1097000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGGTCATCGTGTATTGGGTATTGGATTTGGTGGGAGCTGAGTTTCCATGGACAGAAAATAGCACCAATATTTTATGCTGCGAATTGCATGACCCCAGAAGAAGACAACACCAACAACACGAAGAAGAGGATTCAACAAAAGCAAAAAAACAAGAAAGTTTCTCAAGCAATATGGCTTGTAGCTACTCCAAGATTTCCCCAATCATTTCCATTCTTGTTTGCTTCCTTCTGGTTGCTTCTTCAATTTGTCCGGTGGCACAATCTTCTGATATCCACCGCGAAAGCACTGCCAATCAGACGTTGAATCCGGAAAAAGAACTGAAGAAGTTGAAGATTATGAGAGCTCGTCTTAACAAGATAAACAAGCCTGCTTTCAAGACAATTCAGAGTCCGGATGGCGATCTTATAGATTGTGTTCTTTCTCATCACCAACCAGCTTTTGATCATCCCCAGTTAAAGGGACAGAAGCCATTGGACCCGCCGGAGAGACCGAAAGGCCGGAGCCCTCGAGAAATGGCGACTGAAAACTACCAGCTGTGGAGCATGTCAGGAAATCTCTGCCCACAAGGAACAGTTCCAATTAGAAGAACGAGAGAGGAGGATATGATGAGAGCAAGCTCTGTTAAAAGATTTGGAAGAAAACTAAGTAGGCATGTCAGAAGAGATACATCCAGCAACGGCCATGAGCATGCGGTTGGGTATGTGAGTGGAGATCAATACTATGGAGCCAAAGCAAGCATCAATGTGTGGGCCCCCACAGTTGTTAATCAATACGAATTTAGCTTGTCTCAATTGTGGGTCATCTCTGGTTCTTTTGGTGATGATCTCAACACCATTGAAGCTGGTTGGCAGGTCAGCCCGGAGCTATATGGGGACAACTACCCCAGATTCTTTACTTATTGGACTACGGATGCATATCAAGCAACTGGATGCTACAATTTGCTGTGTTCGGGTTTTGTTCAGACCAACAGTAGAATCGCCATTGGAGCTGCAATTTCTCCAACTTCTTCCTACCATGGTGGACAGTTTGATATTAGCTTGTTGATTTGGAAGGATCCGAAGCATGGAAATTGGTGGCTGGAATTCGGGAACGGTGTCCTAGTCGGATACTGGCCATCATTCTTGTTCACTCACCTTCAAGACCACGCAAGCATGGTACAATTCGGTGGAGAAGTTGTGAACTCGAGGCCTTCGGGCTCCCACACAGCTACACAGATGGGCAGTGGGCACTTTGCAGGTGAAGGGTTTGGAAAAGCTTCATATTTTCGAAACATGCAGGTTGTGGATTGGGATAACAGCTTAATCCCATTATCCAACCTTAAAGTTTTGGCTGATCATCCAAATTGCTACGACATTCAAGGAGGGATTAACAACTTTTGGGGGAATTATTTTTACTATGGAGGTCCTGGAAGAAATGTGAGGTGTCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Neprosin Neprosin_AP

Protein Analysis

477

Amino Acids

53.6

Weight (kDa)

7.25

Isoelectric Point (pI)

38.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Neprosin_AP	PF14365	127 - 234	1.3e-37	Neprosin activation peptide
Neprosin	PF03080	258 - 469	5.6e-87	Neprosin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006525)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10750 AT1G10750 AT1G23340 AT1G23340 AT1G23340 AT1G70550 AT1G70550
fragaria_vesca	FvH4_4g25820 FvH4_4g25820 FvH4_4g25820 FvH4_4g25820
malus_domestica	MD13G1097000.v1.1 MD16G1097900.v1.1
prunus_persica	Prupe.1G248600_v2.0.a1
pyrus_communis	pycom13g08470 pycom16g08340
rosa_chinensis	RchiOBHm_Chr4g0433271
rosa_laevigata	RLG00000006768
rosa_multiflora	Rmu_sc0022950.1_g000002
rosa_roxburghii	Rroxscaffold_5G00374830
rosa_rugosa	Rorug04G0267600
rosa_samantha	Rh4AG322100 Rh4BG329500 Rh4CG344800 Rh4DG326200
rosa_wichuraiana	Rw4G027870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1226
Acc36I	ACCTGC	3 cut(s)	841, 1226, 1267
AccII	CGCG	1 cut(s)	279
AccIII	TCCGGA	2 cut(s)	307, 391
AciI	CCGC	3 cut(s)	277, 479, 692
AclWI	GGATC	2 cut(s)	1049, 1062
AcoI	YGGCCR	2 cut(s)	679, 1112
AcsI	RAATTY	2 cut(s)	776, 1082
AcuI	CTGAAG	1 cut(s)	341
AfaI	GTAC	1 cut(s)	1158
AfiI	CCNNNNNNNGG	2 cut(s)	457, 1064
AgsI	TTSAA	8 cut(s)	135, 243, 304, 331, 376, 839, 1139, 1358
AjnI	CCWGG	1 cut(s)	1405
Alw21I	GWGCWC	1 cut(s)	346
Alw26I	GTCTC	2 cut(s)	481, 792
AlwI	GGATC	2 cut(s)	1049, 1062
Ama87I	CYCGRG	2 cut(s)	507, 1183
Aor13HI	TCCGGA	2 cut(s)	307, 391
AoxI	GGCC	5 cut(s)	496, 679, 750, 1112, 1187
ApaI	GGGCCC	1 cut(s)	754
ApeKI	GCWGC	2 cut(s)	78, 989
ApoI	RAATTY	2 cut(s)	776, 1082
Asp700I	GAANNNNTTC	1 cut(s)	1255
AspS9I	GGNCC	4 cut(s)	475, 750, 751, 1403
AsuC2I	CCSGG	1 cut(s)	860
AsuHPI	GGTGA	4 cut(s)	419, 830, 1124, 1250
AsuII	TTCGAA	1 cut(s)	1267
AvaI	CYCGRG	2 cut(s)	507, 1183
AvaII	GGWCC	2 cut(s)	475, 1403
BaeGI	GKGCMC	2 cut(s)	754, 1230
BalI	TGGCCA	1 cut(s)	1114
BamHI	GGATCC	1 cut(s)	1054
BanII	GRGCYC	4 cut(s)	346, 506, 754, 1199
BbsI	GAAGAC	1 cut(s)	109
Bbv12I	GWGCWC	1 cut(s)	346
BbvI	GCAGC	2 cut(s)	65, 976
BccI	CCATC	3 cut(s)	389, 1123, 1210
BceAI	ACGGC	1 cut(s)	694
BcgI	CGANNNNNNTGC	2 cut(s)	269, 303
BciT130I	CCWGG	1 cut(s)	1407
BciVI	GTATCC	1 cut(s)	1100
BclI	TGATCA	2 cut(s)	442, 1333
BcnI	CCSGG	1 cut(s)	860
BcoDI	GTCTC	2 cut(s)	481, 792
BfaI	CTAG	1 cut(s)	1100
BfuAI	ACCTGC	3 cut(s)	841, 1226, 1267
BfuI	GTATCC	1 cut(s)	1100
BisI	GCNGC	2 cut(s)	79, 990
BlsI	GCNGC	2 cut(s)	80, 991
Bme1390I	CCNGG	2 cut(s)	860, 1407
Bme18I	GGWCC	2 cut(s)	475, 1403
BmeT110I	CYCGRG	2 cut(s)	507, 1183
BmgT120I	GGNCC	4 cut(s)	475, 750, 751, 1403
BmiI	GGNNCC	7 cut(s)	477, 503, 727, 752, 753, 1056, 1198
BmrFI	CCNGG	2 cut(s)	860, 1407
BmrI	ACTGGG	1 cut(s)	445
BmsI	GCATC	3 cut(s)	747, 901, 920
BmuI	ACTGGG	1 cut(s)	445
BpiI	GAAGAC	1 cut(s)	109
Bpu14I	TTCGAA	1 cut(s)	1267
BpuEI	CTTGAG	1 cut(s)	148
BpuMI	CCSGG	1 cut(s)	860
BsaBI	GATNNNNATC	2 cut(s)	399, 915
BsaI	GGTCTC	1 cut(s)	481
BsaJI	CCNNGG	2 cut(s)	47, 1015
BsaWI	WCCGGW	3 cut(s)	250, 307, 391
BsaXI	ACNNNNNCTCC	2 cut(s)	1161, 1191
Bsc4I	CCNNNNNNNGG	2 cut(s)	457, 1064
Bse1I	ACTGG	3 cut(s)	451, 931, 1115
Bse8I	GATNNNNATC	2 cut(s)	399, 915
BseAI	TCCGGA	2 cut(s)	307, 391
BseBI	CCWGG	1 cut(s)	1407
BseDI	CCNNGG	2 cut(s)	47, 1015
BseGI	GGATG	6 cut(s)	400, 445, 668, 916, 935, 1336
BseJI	GATNNNNATC	2 cut(s)	399, 915
BseLI	CCNNNNNNNGG	2 cut(s)	457, 1064
BseMII	CTCAG	1 cut(s)	30
BseNI	ACTGG	3 cut(s)	451, 931, 1115
BseRI	GAGGAG	1 cut(s)	611
BseSI	GKGCMC	2 cut(s)	754, 1230
BseXI	GCAGC	2 cut(s)	65, 976
Bsh1236I	CGCG	1 cut(s)	279
BshFI	GGCC	5 cut(s)	498, 681, 752, 1114, 1189
BsiHKAI	GWGCWC	1 cut(s)	346
BsiHKCI	CYCGRG	2 cut(s)	507, 1183
BsiSI	CCGG	6 cut(s)	251, 308, 392, 482, 499, 860
BslFI	GGGAC	3 cut(s)	474, 887, 1410
BslI	CCNNNNNNNGG	2 cut(s)	457, 1064
BsmAI	GTCTC	2 cut(s)	481, 792
BsmFI	GGGAC	3 cut(s)	474, 887, 1410
BsnI	GGCC	5 cut(s)	498, 681, 752, 1114, 1189
Bso31I	GGTCTC	1 cut(s)	481
BsoBI	CYCGRG	2 cut(s)	507, 1183
Bsp119I	TTCGAA	1 cut(s)	1267
Bsp120I	GGGCCC	1 cut(s)	750
Bsp1286I	GDGCHC	5 cut(s)	346, 506, 754, 1199, 1230
Bsp13I	TCCGGA	2 cut(s)	307, 391
Bsp143I	GATC	6 cut(s)	400, 442, 712, 823, 1054, 1333
Bsp19I	CCATGG	2 cut(s)	47, 1015
BspACI	CCGC	3 cut(s)	277, 479, 692
BspANI	GGCC	5 cut(s)	498, 681, 752, 1114, 1189
BspCNI	CTCAG	1 cut(s)	31
BspEI	TCCGGA	2 cut(s)	307, 391
BspFNI	CGCG	1 cut(s)	279
BspLI	GGNNCC	7 cut(s)	477, 503, 727, 752, 753, 1056, 1198
BspMI	ACCTGC	3 cut(s)	841, 1226, 1267
BspPI	GGATC	2 cut(s)	1049, 1062
BspT104I	TTCGAA	1 cut(s)	1267
BspTNI	GGTCTC	1 cut(s)	481
BsrI	ACTGG	3 cut(s)	451, 931, 1115
BssECI	CCNNGG	2 cut(s)	47, 1015
BssMI	GATC	6 cut(s)	400, 442, 712, 823, 1054, 1333
BssT1I	CCWWGG	2 cut(s)	47, 1015
Bst2UI	CCWGG	1 cut(s)	1407
Bst4CI	ACNGT	5 cut(s)	574, 760, 971, 1026, 1094
Bst6I	CTCTTC	2 cut(s)	120, 655
BstBI	TTCGAA	1 cut(s)	1267
BstC8I	GCNNGC	5 cut(s)	369, 616, 690, 736, 1150
BstDEI	CTNAG	2 cut(s)	39, 644
BstDSI	CCRYGG	2 cut(s)	47, 1015
BstF5I	GGATG	6 cut(s)	400, 445, 668, 916, 935, 1336
BstFNI	CGCG	1 cut(s)	279
BstKTI	GATC	6 cut(s)	403, 445, 715, 826, 1057, 1336
BstMAI	GTCTC	2 cut(s)	481, 792
BstMBI	GATC	6 cut(s)	400, 442, 712, 823, 1054, 1333
BstMWI	GCNNNNNNNGC	2 cut(s)	920, 986
BstNI	CCWGG	1 cut(s)	1407
BstNSI	RCATGY	4 cut(s)	547, 656, 692, 1276
BstSCI	CCNGG	2 cut(s)	858, 1405
BstSLI	GKGCMC	2 cut(s)	754, 1230
BstUI	CGCG	1 cut(s)	279
BstV1I	GCAGC	2 cut(s)	65, 976
BstV2I	GAAGAC	1 cut(s)	109
BstX2I	RGATCY	1 cut(s)	1054
BstYI	RGATCY	1 cut(s)	1054
BsuI	GTATCC	1 cut(s)	1100
BsuRI	GGCC	5 cut(s)	498, 681, 752, 1114, 1189
BtgI	CCRYGG	2 cut(s)	47, 1015
BtsCI	GGATG	6 cut(s)	400, 445, 668, 916, 935, 1336
BtsI	GCAGTG	2 cut(s)	285, 1228
BtsIMutI	CAGTG	2 cut(s)	285, 1228
BveI	ACCTGC	3 cut(s)	841, 1226, 1267
Cac8I	GCNNGC	5 cut(s)	369, 616, 690, 736, 1150
Cfr13I	GGNCC	4 cut(s)	475, 750, 751, 1403
Csp6I	GTAC	1 cut(s)	1157
CviQI	GTAC	1 cut(s)	1157
DdeI	CTNAG	2 cut(s)	39, 644
DpnI	GATC	6 cut(s)	402, 444, 714, 825, 1056, 1335
DpnII	GATC	6 cut(s)	400, 442, 712, 823, 1054, 1333
EaeI	YGGCCR	2 cut(s)	679, 1112
Eam1104I	CTCTTC	2 cut(s)	120, 655
EarI	CTCTTC	2 cut(s)	120, 655
Ecl136II	GAGCTC	1 cut(s)	344
Eco130I	CCWWGG	2 cut(s)	47, 1015
Eco147I	AGGCCT	1 cut(s)	1189
Eco24I	GRGCYC	4 cut(s)	346, 506, 754, 1199
Eco31I	GGTCTC	1 cut(s)	481
Eco32I	GATATC	1 cut(s)	271
Eco47I	GGWCC	2 cut(s)	475, 1403
Eco53kI	GAGCTC	1 cut(s)	344
Eco57I	CTGAAG	1 cut(s)	341
Eco88I	CYCGRG	2 cut(s)	507, 1183
EcoICRI	GAGCTC	1 cut(s)	344
EcoO109I	RGGNCCY	2 cut(s)	751, 1403
EcoRI	GAATTC	1 cut(s)	1082
EcoRII	CCWGG	1 cut(s)	1405
EcoRV	GATATC	1 cut(s)	271
EcoT14I	CCWWGG	2 cut(s)	47, 1015
EcoT22I	ATGCAT	1 cut(s)	916
EcoT38I	GRGCYC	4 cut(s)	346, 506, 754, 1199
ErhI	CCWWGG	2 cut(s)	47, 1015
FaqI	GGGAC	3 cut(s)	474, 887, 1410
FauI	CCCGC	1 cut(s)	486
FbaI	TGATCA	2 cut(s)	442, 1333
Fnu4HI	GCNGC	2 cut(s)	79, 990
FokI	GGATG	6 cut(s)	407, 432, 655, 923, 942, 1323
FriOI	GRGCYC	4 cut(s)	346, 506, 754, 1199
Fsp4HI	GCNGC	2 cut(s)	79, 990
FspBI	CTAG	1 cut(s)	1100
GluI	GCNGC	2 cut(s)	79, 990
HaeIII	GGCC	5 cut(s)	498, 681, 752, 1114, 1189
HapII	CCGG	6 cut(s)	251, 308, 392, 482, 499, 860
HindIII	AAGCTT	1 cut(s)	1254
HinfI	GANTC	5 cut(s)	131, 304, 388, 888, 975
HpaII	CCGG	6 cut(s)	251, 308, 392, 482, 499, 860
HphI	GGTGA	4 cut(s)	419, 830, 1124, 1250
Hpy166II	GTNNAC	3 cut(s)	1022, 1128, 1180
Hpy188I	TCNGA	7 cut(s)	268, 297, 387, 659, 963, 1059, 1106
Hpy188III	TCNNGA	7 cut(s)	308, 376, 392, 509, 549, 1087, 1139
Hpy8I	GTNNAC	3 cut(s)	1022, 1128, 1180
HpyAV	CCTTC	5 cut(s)	236, 1045, 1145, 1200, 1235
HpyCH4III	ACNGT	5 cut(s)	574, 760, 971, 1026, 1094
HpyCH4IV	ACGT	1 cut(s)	299
HpyCH4V	TGCA	5 cut(s)	88, 914, 992, 1235, 1276
HpyF10VI	GCNNNNNNNGC	2 cut(s)	920, 986
HpyF3I	CTNAG	2 cut(s)	39, 644
HpySE526I	ACGT	1 cut(s)	299
Kpn2I	TCCGGA	2 cut(s)	307, 391
Ksp22I	TGATCA	2 cut(s)	442, 1333
Kzo9I	GATC	6 cut(s)	400, 442, 712, 823, 1054, 1333
LmnI	GCTCC	7 cut(s)	35, 501, 540, 725, 862, 986, 1202
Lsp1109I	GCAGC	2 cut(s)	65, 976
LweI	GCATC	3 cut(s)	747, 901, 920
MaeI	CTAG	1 cut(s)	1100
MaeII	ACGT	1 cut(s)	299
MalI	GATC	6 cut(s)	402, 444, 714, 825, 1056, 1335
MboI	GATC	6 cut(s)	400, 442, 712, 823, 1054, 1333
MfeI	CAATTG	1 cut(s)	791
MflI	RGATCY	1 cut(s)	1054
MhlI	GDGCHC	5 cut(s)	346, 506, 754, 1199, 1230
MlsI	TGGCCA	1 cut(s)	1114
MluNI	TGGCCA	1 cut(s)	1114
MlyI	GAGTC	1 cut(s)	397
MmeI	TCCRAC	3 cut(s)	1025, 1084, 1338
MnlI	CCTC	8 cut(s)	121, 516, 589, 592, 1179, 1355, 1394, 1413
Mox20I	TGGCCA	1 cut(s)	1114
Mph1103I	ATGCAT	1 cut(s)	916
MroI	TCCGGA	2 cut(s)	307, 391
MroXI	GAANNNNTTC	1 cut(s)	1255
MscI	TGGCCA	1 cut(s)	1114
MseI	TTAA	7 cut(s)	351, 455, 624, 765, 1301, 1320, 1368
MslI	CAYNNNNRTG	2 cut(s)	687, 743
Msp20I	TGGCCA	1 cut(s)	1114
MspA1I	CMGCKG	1 cut(s)	535
MspI	CCGG	6 cut(s)	251, 308, 392, 482, 499, 860
MspR9I	CCNGG	2 cut(s)	860, 1407
MunI	CAATTG	1 cut(s)	791
MvaI	CCWGG	1 cut(s)	1407
MvnI	CGCG	1 cut(s)	279
MwoI	GCNNNNNNNGC	2 cut(s)	920, 986
NciI	CCSGG	1 cut(s)	860
NcoI	CCATGG	2 cut(s)	47, 1015
NdeII	GATC	6 cut(s)	400, 442, 712, 823, 1054, 1333
NlaIV	GGNNCC	7 cut(s)	477, 503, 727, 752, 753, 1056, 1198
NsiI	ATGCAT	1 cut(s)	916
NspI	RCATGY	4 cut(s)	547, 656, 692, 1276
NspV	TTCGAA	1 cut(s)	1267
PaeI	GCATGC	1 cut(s)	692
PaeR7I	CTCGAG	2 cut(s)	507, 1183
PaqCI	CACCTGC	1 cut(s)	1226
PceI	AGGCCT	1 cut(s)	1189
PdmI	GAANNNNTTC	1 cut(s)	1255
PfeI	GAWTC	4 cut(s)	131, 304, 888, 975
PfoI	TCCNGGA	1 cut(s)	1405
PkrI	GCNGC	2 cut(s)	80, 991
PleI	GAGTC	1 cut(s)	396
PpsI	GAGTC	1 cut(s)	396
PpuMI	RGGWCCY	1 cut(s)	1403
Psp124BI	GAGCTC	1 cut(s)	346
Psp5II	RGGWCCY	1 cut(s)	1403
Psp6I	CCWGG	1 cut(s)	1405
PspGI	CCWGG	1 cut(s)	1405
PspN4I	GGNNCC	7 cut(s)	477, 503, 727, 752, 753, 1056, 1198
PspOMI	GGGCCC	1 cut(s)	750
PspPI	GGNCC	4 cut(s)	475, 750, 751, 1403
PspPPI	RGGWCCY	1 cut(s)	1403
PspXI	VCTCGAGB	1 cut(s)	1183
PsuI	RGATCY	1 cut(s)	1054
PvuII	CAGCTG	1 cut(s)	535
RsaI	GTAC	1 cut(s)	1158
RsaNI	GTAC	1 cut(s)	1157
RseI	CAYNNNNRTG	2 cut(s)	687, 743
SacI	GAGCTC	1 cut(s)	346
SaqAI	TTAA	7 cut(s)	351, 455, 624, 765, 1301, 1320, 1368
SatI	GCNGC	2 cut(s)	79, 990
Sau3AI	GATC	6 cut(s)	400, 442, 712, 823, 1054, 1333
Sau96I	GGNCC	4 cut(s)	475, 750, 751, 1403
SchI	GAGTC	1 cut(s)	397
ScrFI	CCNGG	2 cut(s)	860, 1407
SduI	GDGCHC	5 cut(s)	346, 506, 754, 1199, 1230
SfaNI	GCATC	3 cut(s)	747, 901, 920
Sfr274I	CTCGAG	2 cut(s)	507, 1183
SfuI	TTCGAA	1 cut(s)	1267
SinI	GGWCC	2 cut(s)	475, 1403
SlaI	CTCGAG	2 cut(s)	507, 1183
SmiMI	CAYNNNNRTG	2 cut(s)	687, 743
SmlI	CTYRAG	3 cut(s)	163, 507, 1183
SmoI	CTYRAG	3 cut(s)	163, 507, 1183
SphI	GCATGC	1 cut(s)	692
SseBI	AGGCCT	1 cut(s)	1189
SsiI	CCGC	3 cut(s)	277, 479, 692
SspI	AATATT	1 cut(s)	70
SspMI	CTAG	1 cut(s)	1100
SstI	GAGCTC	1 cut(s)	346
StuI	AGGCCT	1 cut(s)	1189
StyD4I	CCNGG	2 cut(s)	858, 1405
StyI	CCWWGG	2 cut(s)	47, 1015
TaaI	ACNGT	5 cut(s)	574, 760, 971, 1026, 1094
TaiI	ACGT	1 cut(s)	302
TaqI	TCGA	3 cut(s)	508, 1184, 1267
TaqII	GACCGA	1 cut(s)	505
TfiI	GAWTC	4 cut(s)	131, 304, 888, 975
Tru1I	TTAA	7 cut(s)	351, 455, 624, 765, 1301, 1320, 1368
Tru9I	TTAA	7 cut(s)	351, 455, 624, 765, 1301, 1320, 1368
TscAI	CASTG	2 cut(s)	292, 1228
TseI	GCWGC	2 cut(s)	78, 989
TspDTI	ATGAA	1 cut(s)	1248
TspGWI	ACGGA	1 cut(s)	923
TspRI	CASTG	2 cut(s)	292, 1228
VpaK11BI	GGWCC	2 cut(s)	475, 1403
XapI	RAATTY	2 cut(s)	776, 1082
XceI	RCATGY	4 cut(s)	547, 656, 692, 1276
XhoI	CTCGAG	2 cut(s)	507, 1183
XmnI	GAANNNNTTC	1 cut(s)	1255
XspI	CTAG	1 cut(s)	1100
Zsp2I	ATGCAT	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.