FvH4_4g26160

Repressor of RNA polymerase III transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010817
Physical Location & Seq
Forward (+)
27676560 .. 27679800
3241 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26160.t1

Sequence Viewer

Length: 675 bp
ATGAAGTTCTTGGAGTACACACCCTTGGATCGATTAAATGATTTCTTTAGTCATTTAAATCTTGGAGAGAGAACTATTAAGGGCTGTCTTGAAGCTTACTCTTGTAAACACACGGGTTTGGATAAGAAATTGTCTCTGAGTTTGGAGAATGAGATTCTTGACTATCTCGTAAAATCTTCTGACACTGACTGTTCCTCGCCCGCTGAGTCCCTTCTGAGTAGATCCAGTCGGAAGACATTGATTTACCTGGTCTTGACCCTCTATCACTTGTATCCGGACTATGACTTCAGTGCTATCAATGCTCACCAGTTCTTCACAGAGGAAACCTGGGACAGTTTTAAGCAGATTTTTGATACCTATATGGTGGAAGCATCAAAGGAATGGACAGAGACAAATGAGGGTAGTTCCCTGCTGGATACCTTGTACAAGGCTTTGGATGAGGTTGTTGGATTAGCAGAATGCGAAATTTATGGTTACAATCCTGATCCTGATGCTGATCCGTTTCCCGAAAGAGGAGCTGTATGGTCATTCAATTTCTTTTTCTACAATAGAAAACTGAAACGGGTCGTTACCTTCCGTATCTGCTGTTTAAGTAACTTGGTGACTGACGGGTTTATAATGGATGAACTCCGTCACGAGGAAGATGGAGAAATTTTTGATGTCATGGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf1

Protein Analysis

225

Amino Acids

26.04

Weight (kDa)

4.53

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf1 PF09174 26 - 192 1.7e-50 Maf1 regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 617
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 1 cut(s) 201
AclWI GGATC 4 cut(s) 36, 216, 479, 491
AcsI RAATTY 2 cut(s) 465, 651
AcuI CTGAAG 1 cut(s) 271
AfaI GTAC 2 cut(s) 17, 425
AfiI CCNNNNNNNGG 2 cut(s) 512, 637
AgsI TTSAA 2 cut(s) 92, 532
AjnI CCWGG 2 cut(s) 246, 326
AluBI AGCT 2 cut(s) 95, 518
AluI AGCT 2 cut(s) 95, 518
Alw26I GTCTC 2 cut(s) 138, 383
AlwI GGATC 4 cut(s) 36, 216, 479, 491
Aor13HI TCCGGA 1 cut(s) 274
ApoI RAATTY 2 cut(s) 465, 651
AsuHPI GGTGA 2 cut(s) 296, 613
BauI CACGAG 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 239
BccI CCATC 1 cut(s) 638
BciT130I CCWGG 2 cut(s) 248, 328
BciVI GTATCC 2 cut(s) 282, 409
BcoDI GTCTC 2 cut(s) 138, 383
BfuI GTATCC 2 cut(s) 282, 409
Bme1390I CCNGG 2 cut(s) 248, 328
BmrFI CCNGG 2 cut(s) 248, 328
BmsI GCATC 2 cut(s) 380, 481
BpiI GAAGAC 1 cut(s) 239
Bsa29I ATCGAT 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 24, 327
BsaWI WCCGGW 1 cut(s) 274
Bsc4I CCNNNNNNNGG 2 cut(s) 512, 637
Bse1I ACTGG 2 cut(s) 225, 307
Bse8I GATNNNNATC 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 274
BseBI CCWGG 2 cut(s) 248, 328
BseCI ATCGAT 1 cut(s) 31
BseDI CCNNGG 2 cut(s) 24, 327
BseGI GGATG 2 cut(s) 442, 628
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 2 cut(s) 512, 637
BseMII CTCAG 3 cut(s) 128, 195, 206
BseNI ACTGG 2 cut(s) 225, 307
BseRI GAGGAG 1 cut(s) 528
BshVI ATCGAT 1 cut(s) 31
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 2 cut(s) 193, 344
BslI CCNNNNNNNGG 2 cut(s) 512, 637
BsmAI GTCTC 2 cut(s) 138, 383
BsmFI GGGAC 2 cut(s) 193, 344
BsmI GAATGC 1 cut(s) 464
Bsp13I TCCGGA 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 423
Bsp143I GATC 4 cut(s) 28, 221, 484, 496
BspACI CCGC 1 cut(s) 201
BspCNI CTCAG 3 cut(s) 129, 196, 207
BspDI ATCGAT 1 cut(s) 31
BspEI TCCGGA 1 cut(s) 274
BspPI GGATC 4 cut(s) 36, 216, 479, 491
BsrGI TGTACA 1 cut(s) 423
BsrI ACTGG 2 cut(s) 225, 307
BssECI CCNNGG 2 cut(s) 24, 327
BssMI GATC 4 cut(s) 28, 221, 484, 496
BssSI CACGAG 1 cut(s) 635
BssT1I CCWWGG 1 cut(s) 24
Bst2BI CACGAG 1 cut(s) 635
Bst2UI CCWGG 2 cut(s) 248, 328
Bst4CI ACNGT 2 cut(s) 191, 335
BstAUI TGTACA 1 cut(s) 423
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 3 cut(s) 137, 204, 215
BstF5I GGATG 2 cut(s) 442, 628
BstKTI GATC 4 cut(s) 31, 224, 487, 499
BstMAI GTCTC 2 cut(s) 138, 383
BstMBI GATC 4 cut(s) 28, 221, 484, 496
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNI CCWGG 2 cut(s) 248, 328
BstSCI CCNGG 2 cut(s) 246, 326
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
Bsu15I ATCGAT 1 cut(s) 31
BsuI GTATCC 2 cut(s) 282, 409
BsuTUI ATCGAT 1 cut(s) 31
BtsCI GGATG 2 cut(s) 442, 628
BtsIMutI CAGTG 2 cut(s) 183, 295
Cac8I GCNNGC 1 cut(s) 201
ClaI ATCGAT 1 cut(s) 31
CsiI ACCWGGT 1 cut(s) 246
Csp6I GTAC 2 cut(s) 16, 424
CviAII CATG 1 cut(s) 664
CviJI RGCY 4 cut(s) 84, 95, 431, 518
CviKI_1 RGCY 4 cut(s) 84, 95, 431, 518
CviQI GTAC 2 cut(s) 16, 424
DdeI CTNAG 3 cut(s) 137, 204, 215
DpnI GATC 4 cut(s) 30, 223, 486, 498
DpnII GATC 4 cut(s) 28, 221, 484, 496
DraI TTTAAA 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 246, 326
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 1 cut(s) 667
FaiI YATR 8 cut(s) 282, 360, 362, 471, 523, 617, 665, 671
FaqI GGGAC 2 cut(s) 193, 344
FatI CATG 1 cut(s) 663
FauI CCCGC 1 cut(s) 208
FokI GGATG 2 cut(s) 449, 635
HapII CCGG 1 cut(s) 275
Hin1II CATG 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 2 cut(s) 154, 206
HpaII CCGG 1 cut(s) 275
HphI GGTGA 2 cut(s) 296, 613
Hpy166II GTNNAC 2 cut(s) 18, 107
Hpy188I TCNGA 4 cut(s) 138, 181, 216, 231
Hpy188III TCNNGA 8 cut(s) 89, 158, 253, 275, 482, 488, 506, 635
Hpy8I GTNNAC 2 cut(s) 18, 107
HpyAV CCTTC 2 cut(s) 221, 583
HpyCH4III ACNGT 2 cut(s) 191, 335
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 3 cut(s) 137, 204, 215
Hsp92II CATG 1 cut(s) 667
Kpn2I TCCGGA 1 cut(s) 274
Kzo9I GATC 4 cut(s) 28, 221, 484, 496
LmnI GCTCC 1 cut(s) 515
LweI GCATC 2 cut(s) 380, 481
MabI ACCWGGT 1 cut(s) 246
MaeIII GTNAC 5 cut(s) 473, 568, 593, 601, 632
MalI GATC 4 cut(s) 30, 223, 486, 498
MboI GATC 4 cut(s) 28, 221, 484, 496
MboII GAAGA 4 cut(s) 168, 244, 304, 653
MflI RGATCY 1 cut(s) 221
MluCI AATT 4 cut(s) 128, 465, 532, 651
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 2 cut(s) 209, 427
MnlI CCTC 7 cut(s) 205, 269, 313, 391, 433, 506, 631
MroI TCCGGA 1 cut(s) 274
MseI TTAA 5 cut(s) 35, 56, 78, 339, 590
MslI CAYNNNNRTG 1 cut(s) 668
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 248, 328
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 2 cut(s) 248, 328
MwoI GCNNNNNNNGC 1 cut(s) 299
NdeII GATC 4 cut(s) 28, 221, 484, 496
NlaIII CATG 1 cut(s) 667
NmuCI GTSAC 2 cut(s) 601, 632
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 154
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PsiI TTATAA 1 cut(s) 617
Psp6I CCWGG 2 cut(s) 246, 326
PspGI CCWGG 2 cut(s) 246, 326
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 2 cut(s) 17, 425
RsaNI GTAC 2 cut(s) 16, 424
RseI CAYNNNNRTG 1 cut(s) 668
SaqAI TTAA 5 cut(s) 35, 56, 78, 339, 590
Sau3AI GATC 4 cut(s) 28, 221, 484, 496
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 2 cut(s) 248, 328
SetI ASST 8 cut(s) 97, 249, 329, 359, 422, 444, 520, 575
SexAI ACCWGGT 1 cut(s) 246
SfaNI GCATC 2 cut(s) 380, 481
SmiI ATTTAAAT 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 668
Sse9I AATT 4 cut(s) 128, 465, 532, 651
SsiI CCGC 1 cut(s) 201
StyD4I CCNGG 2 cut(s) 246, 326
StyI CCWWGG 1 cut(s) 24
SwaI ATTTAAAT 1 cut(s) 57
TaaI ACNGT 2 cut(s) 191, 335
TaqI TCGA 1 cut(s) 31
TasI AATT 4 cut(s) 128, 465, 532, 651
TatI WGTACW 2 cut(s) 15, 423
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 5 cut(s) 35, 56, 78, 339, 590
Tru9I TTAA 5 cut(s) 35, 56, 78, 339, 590
TscAI CASTG 2 cut(s) 190, 295
TseFI GTSAC 2 cut(s) 601, 632
Tsp45I GTSAC 2 cut(s) 601, 632
TspDTI ATGAA 2 cut(s) 17, 639
TspGWI ACGGA 3 cut(s) 489, 566, 620
TspRI CASTG 2 cut(s) 190, 295
XapI RAATTY 2 cut(s) 465, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.