MD13G1028600.v1.1

Repressor of RNA polymerase III transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010817
Physical Location & Seq
Forward (+)
2037422 .. 2040631
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1028600.v1.1.491

Sequence Viewer

Length: 675 bp
ATGAAGTTTTTGGAATACACCCCGCTGGAGCGATTAAATGATTTCTTTAGTCATTTGAATCTCGGAGAGCGAACCATTAAGGGTTGTCTTGAAGCTTACTCTTGCAAACACACCGGATCCGATAAGAAATTGTCTCTCAGTTTGGAGAATGAGATTCTTGATTATCTTGGGAAATCGTCTGACAGTGATTCTTCCTCGCCTGCTGAATTCCTGCTGACTAGATCCAGCCGGAAGACATTGATTTACCTGGTTTTGACACTCTATCACTTGTATCCGGACTATGACTTCAGTGCAGTAAATGCTCATCAGTTCTTCACAGAGGAAAGCTGGGACAGTTTTAAGCAGATTTTTGATACCTACATGTTTGAAGCATCAAAGGAATGGACAGAGACAAATGAGGGCAGTTCCTTGCTGGAAGCCCTTTACAAGGCTTTGGATGAGGTTGTTAGACTAGCGGAATGTGAAGTTTACAGTTACATTCCAGACTCTGATGCCGATCCGTTTCTTGAGAGAGGATCTATATGGTCGTTCAATTTTTTTTTCTACAATAGGAAACTTAAGCGTGTCGTGAGCTTCCGTGTCTGCTGTTTAAGTAACTTGGTGGCTGATGGATATCTGATGGACGAGTTGGGGTATGATGGAGATGGAGAAATTTTCGACAACATGGACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf1

Protein Analysis

225

Amino Acids

25.98

Weight (kDa)

4.52

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf1 PF09174 26 - 192 1.8e-52 Maf1 regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 2 cut(s) 23, 455
AclWI GGATC 5 cut(s) 111, 124, 216, 491, 523
AcsI RAATTY 2 cut(s) 206, 651
AcuI CTGAAG 1 cut(s) 271
AfiI CCNNNNNNNGG 1 cut(s) 427
AflII CTTAAG 1 cut(s) 557
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 4 cut(s) 58, 92, 368, 532
AjnI CCWGG 1 cut(s) 246
AluBI AGCT 3 cut(s) 95, 327, 573
AluI AGCT 3 cut(s) 95, 327, 573
Alw26I GTCTC 2 cut(s) 138, 383
AlwI GGATC 5 cut(s) 111, 124, 216, 491, 523
AlwNI CAGNNNCTG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 274
ApoI RAATTY 2 cut(s) 206, 651
BamHI GGATCC 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 239
BccI CCATC 4 cut(s) 602, 613, 632, 638
BciT130I CCWGG 1 cut(s) 248
BciVI GTATCC 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 138, 383
BfaI CTAG 2 cut(s) 219, 452
BfrI CTTAAG 1 cut(s) 557
BfuI GTATCC 1 cut(s) 282
Bme1390I CCNGG 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 2 cut(s) 380, 481
BpiI GAAGAC 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 527
BsaBI GATNNNNATC 2 cut(s) 495, 612
BsaWI WCCGGW 2 cut(s) 113, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse8I GATNNNNATC 2 cut(s) 495, 612
BseAI TCCGGA 1 cut(s) 274
BseBI CCWGG 1 cut(s) 248
BseGI GGATG 1 cut(s) 442
BseJI GATNNNNATC 2 cut(s) 495, 612
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMII CTCAG 1 cut(s) 151
BseYI CCCAGC 1 cut(s) 327
BsgI GTGCAG 1 cut(s) 312
BsiSI CCGG 3 cut(s) 114, 229, 275
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 427
BsmAI GTCTC 2 cut(s) 138, 383
BsmFI GGGAC 1 cut(s) 344
Bsp13I TCCGGA 1 cut(s) 274
Bsp143I GATC 4 cut(s) 116, 221, 496, 515
BspACI CCGC 2 cut(s) 23, 455
BspCNI CTCAG 1 cut(s) 150
BspEI TCCGGA 1 cut(s) 274
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 5 cut(s) 111, 124, 216, 491, 523
BspTI CTTAAG 1 cut(s) 557
BssMI GATC 4 cut(s) 116, 221, 496, 515
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 3 cut(s) 185, 335, 473
BstAFI CTTAAG 1 cut(s) 557
BstAPI GCANNNNNTGC 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 137
BstENI CCTNNNNNAGG 1 cut(s) 425
BstF5I GGATG 1 cut(s) 442
BstKTI GATC 4 cut(s) 119, 224, 499, 518
BstMAI GTCTC 2 cut(s) 138, 383
BstMBI GATC 4 cut(s) 116, 221, 496, 515
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 246
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 3 cut(s) 116, 221, 515
BstYI RGATCY 3 cut(s) 116, 221, 515
BsuI GTATCC 1 cut(s) 282
BtsCI GGATG 1 cut(s) 442
BtsIMutI CAGTG 2 cut(s) 190, 295
Cac8I GCNNGC 1 cut(s) 201
CaiI CAGNNNCTG 1 cut(s) 488
CsiI ACCWGGT 1 cut(s) 246
CviAII CATG 2 cut(s) 361, 664
CviJI RGCY 7 cut(s) 95, 228, 327, 419, 431, 573, 605
CviKI_1 RGCY 7 cut(s) 95, 228, 327, 419, 431, 573, 605
DdeI CTNAG 1 cut(s) 137
DpnI GATC 4 cut(s) 118, 223, 498, 517
DpnII GATC 4 cut(s) 116, 221, 496, 515
Eco32I GATATC 1 cut(s) 614
Eco57I CTGAAG 1 cut(s) 271
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 246
EcoRV GATATC 1 cut(s) 614
FaeI CATG 2 cut(s) 364, 667
FaiI YATR 6 cut(s) 282, 362, 521, 523, 636, 665
FaqI GGGAC 1 cut(s) 344
FatI CATG 2 cut(s) 360, 663
FauI CCCGC 1 cut(s) 30
FokI GGATG 1 cut(s) 449
FspBI CTAG 2 cut(s) 219, 452
GsaI CCCAGC 1 cut(s) 331
GsuI CTGGAG 1 cut(s) 47
HapII CCGG 3 cut(s) 114, 229, 275
Hin1II CATG 2 cut(s) 364, 667
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 4 cut(s) 58, 154, 188, 485
HpaII CCGG 3 cut(s) 114, 229, 275
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 5 cut(s) 65, 121, 181, 490, 618
Hpy188III TCNNGA 6 cut(s) 89, 158, 275, 482, 506, 568
Hpy8I GTNNAC 1 cut(s) 469
HpyCH4III ACNGT 3 cut(s) 185, 335, 473
HpyCH4V TGCA 2 cut(s) 105, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 1 cut(s) 137
Hsp92II CATG 2 cut(s) 364, 667
Kpn2I TCCGGA 1 cut(s) 274
Kzo9I GATC 4 cut(s) 116, 221, 496, 515
LmnI GCTCC 1 cut(s) 28
LweI GCATC 2 cut(s) 380, 481
MabI ACCWGGT 1 cut(s) 246
MaeI CTAG 2 cut(s) 219, 452
MaeIII GTNAC 2 cut(s) 473, 593
MalI GATC 4 cut(s) 118, 223, 498, 517
MboI GATC 4 cut(s) 116, 221, 496, 515
MboII GAAGA 3 cut(s) 183, 244, 304
MflI RGATCY 3 cut(s) 116, 221, 515
MluCI AATT 4 cut(s) 128, 206, 532, 651
MlyI GAGTC 1 cut(s) 479
MnlI CCTC 5 cut(s) 205, 313, 391, 433, 506
MroI TCCGGA 1 cut(s) 274
MseI TTAA 5 cut(s) 35, 78, 339, 558, 590
MspA1I CMGCKG 1 cut(s) 25
MspCI CTTAAG 1 cut(s) 557
MspI CCGG 3 cut(s) 114, 229, 275
MspR9I CCNGG 1 cut(s) 248
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 299
NdeII GATC 4 cut(s) 116, 221, 496, 515
NlaIII CATG 2 cut(s) 364, 667
NlaIV GGNNCC 1 cut(s) 118
NspI RCATGY 1 cut(s) 364
PciI ACATGT 1 cut(s) 360
PfeI GAWTC 3 cut(s) 58, 154, 188
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
PscI ACATGT 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 246
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 1 cut(s) 118
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 3 cut(s) 116, 221, 515
SaqAI TTAA 5 cut(s) 35, 78, 339, 558, 590
Sau3AI GATC 4 cut(s) 116, 221, 496, 515
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 1 cut(s) 248
SetI ASST 6 cut(s) 97, 249, 329, 359, 444, 575
SexAI ACCWGGT 1 cut(s) 246
SfaNI GCATC 2 cut(s) 380, 481
SmlI CTYRAG 2 cut(s) 506, 557
SmoI CTYRAG 2 cut(s) 506, 557
Sse9I AATT 4 cut(s) 128, 206, 532, 651
SsiI CCGC 2 cut(s) 23, 455
SspMI CTAG 2 cut(s) 219, 452
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 3 cut(s) 185, 335, 473
TaqI TCGA 1 cut(s) 657
TasI AATT 4 cut(s) 128, 206, 532, 651
TfiI GAWTC 3 cut(s) 58, 154, 188
Tru1I TTAA 5 cut(s) 35, 78, 339, 558, 590
Tru9I TTAA 5 cut(s) 35, 78, 339, 558, 590
TscAI CASTG 2 cut(s) 190, 295
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 489, 566
TspRI CASTG 2 cut(s) 190, 295
Vha464I CTTAAG 1 cut(s) 557
XagI CCTNNNNNAGG 1 cut(s) 425
XapI RAATTY 2 cut(s) 206, 651
XceI RCATGY 1 cut(s) 364
XspI CTAG 2 cut(s) 219, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.