RchiOBHm_Chr4g0433731

Repressor of RNA polymerase III transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010817
Physical Location & Seq
Forward (+)
57749438 .. 57752672
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40220

Sequence Viewer

Length: 675 bp
ATGAAGTACTTAGAATACACACCCTTGGAGCGATTAAATGATTTCTTTAGTCATTTAAATCTTGGAGAGAGAACCATTAAGGGATGTCTTGAAGCTTACTCTTGTAAACACACGGGATCGGATAAGAAACTGTCTCTGAGTTTGGAGAATGAGATTCTTGACTATATCGGAAAATCTTCTGACACCGACTCTTCCTCACCTGCTGAGTTCCTTCTGAGCAGATCCAGCCGGAAGACATTGATTTACCTGGTCTTGACCCTCTATCACTTGTATCCGGACTATGACTTCAGTGCCATCAATGCTCACCAGTTCTTCACAGAGGAAACCTGGGACAGTTTTAAGCAGATTTTTGATACCTATATGGTGGAAGCATCAAAGGAATGGACAGAGACCAATGAGGGTAGTTCCCTGCTGGATACCTTGTACAAGGCTTTGGATGAGGTTGTTGGATTAGCAGATTGCGAAGTTTATGGTTACAATCCTGATCCTGATGCTGATCCGTTTCCGGAAAGAGGAGCTGTATGGTCATTCAATATCTTTTTCTACAATAGAAAACTGAAAAGGGTTGTGAGTTTCCGTATCTGCTGTTTAAGTAACTTGGTGACTGATGGGTTTATAATGGATGATCTCCGTTATGAAGAAGATGGAGAAATTTTTGATGTCATGGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf1

Protein Analysis

224

Amino Acids

25.98

Weight (kDa)

4.47

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf1 PF09174 26 - 192 1.1e-50 Maf1 regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 617
AarI CACCTGC 1 cut(s) 208
Acc36I ACCTGC 1 cut(s) 208
AccIII TCCGGA 2 cut(s) 274, 505
AclWI GGATC 4 cut(s) 124, 216, 479, 491
AcsI RAATTY 1 cut(s) 651
AcuI CTGAAG 1 cut(s) 271
AfaI GTAC 2 cut(s) 8, 425
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 2 cut(s) 92, 532
AjnI CCWGG 2 cut(s) 246, 326
AluBI AGCT 2 cut(s) 95, 518
AluI AGCT 2 cut(s) 95, 518
Alw26I GTCTC 2 cut(s) 138, 383
AlwI GGATC 4 cut(s) 124, 216, 479, 491
Aor13HI TCCGGA 2 cut(s) 274, 505
ApoI RAATTY 1 cut(s) 651
Asp700I GAANNNNTTC 1 cut(s) 175
AsuHPI GGTGA 3 cut(s) 189, 296, 613
BbsI GAAGAC 1 cut(s) 239
BccI CCATC 3 cut(s) 302, 602, 638
BciT130I CCWGG 2 cut(s) 248, 328
BciVI GTATCC 2 cut(s) 282, 409
BcoDI GTCTC 2 cut(s) 138, 383
BfuAI ACCTGC 1 cut(s) 208
BfuI GTATCC 2 cut(s) 282, 409
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 2 cut(s) 248, 328
BmrFI CCNGG 2 cut(s) 248, 328
BmsI GCATC 2 cut(s) 380, 481
BpiI GAAGAC 1 cut(s) 239
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 24, 327
BsaWI WCCGGW 2 cut(s) 274, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse1I ACTGG 1 cut(s) 307
Bse8I GATNNNNATC 1 cut(s) 495
BseAI TCCGGA 2 cut(s) 274, 505
BseBI CCWGG 2 cut(s) 248, 328
BseDI CCNNGG 2 cut(s) 24, 327
BseGI GGATG 3 cut(s) 89, 442, 628
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMII CTCAG 3 cut(s) 128, 195, 206
BseNI ACTGG 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 528
BsiSI CCGG 3 cut(s) 229, 275, 506
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 2 cut(s) 138, 383
BsmFI GGGAC 1 cut(s) 344
Bso31I GGTCTC 1 cut(s) 383
Bsp13I TCCGGA 2 cut(s) 274, 505
Bsp1407I TGTACA 1 cut(s) 423
Bsp143I GATC 5 cut(s) 116, 221, 484, 496, 625
BspCNI CTCAG 3 cut(s) 129, 196, 207
BspEI TCCGGA 2 cut(s) 274, 505
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 4 cut(s) 124, 216, 479, 491
BspTNI GGTCTC 1 cut(s) 383
BsrGI TGTACA 1 cut(s) 423
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 2 cut(s) 24, 327
BssMI GATC 5 cut(s) 116, 221, 484, 496, 625
BssT1I CCWWGG 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 248, 328
Bst4CI ACNGT 2 cut(s) 132, 335
Bst6I CTCTTC 1 cut(s) 196
BstAUI TGTACA 1 cut(s) 423
BstDEI CTNAG 4 cut(s) 10, 137, 204, 215
BstF5I GGATG 3 cut(s) 89, 442, 628
BstKTI GATC 5 cut(s) 119, 224, 487, 499, 628
BstMAI GTCTC 2 cut(s) 138, 383
BstMBI GATC 5 cut(s) 116, 221, 484, 496, 625
BstMWI GCNNNNNNNGC 2 cut(s) 225, 299
BstNI CCWGG 2 cut(s) 248, 328
BstSCI CCNGG 2 cut(s) 246, 326
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
BsuI GTATCC 2 cut(s) 282, 409
BtsCI GGATG 3 cut(s) 89, 442, 628
BtsIMutI CAGTG 1 cut(s) 295
BveI ACCTGC 1 cut(s) 208
CsiI ACCWGGT 1 cut(s) 246
Csp6I GTAC 2 cut(s) 7, 424
CviAII CATG 1 cut(s) 664
CviJI RGCY 4 cut(s) 95, 228, 431, 518
CviKI_1 RGCY 4 cut(s) 95, 228, 431, 518
CviQI GTAC 2 cut(s) 7, 424
DdeI CTNAG 4 cut(s) 10, 137, 204, 215
DpnI GATC 5 cut(s) 118, 223, 486, 498, 627
DpnII GATC 5 cut(s) 116, 221, 484, 496, 625
DraI TTTAAA 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 246, 326
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 1 cut(s) 667
FaqI GGGAC 1 cut(s) 344
FatI CATG 1 cut(s) 663
FokI GGATG 3 cut(s) 96, 449, 635
HapII CCGG 3 cut(s) 229, 275, 506
Hin1II CATG 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 2 cut(s) 154, 188
HpaII CCGG 3 cut(s) 229, 275, 506
HphI GGTGA 3 cut(s) 189, 296, 613
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 5 cut(s) 121, 138, 170, 181, 216
Hpy188III TCNNGA 7 cut(s) 89, 158, 253, 275, 482, 488, 506
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 2 cut(s) 132, 335
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 299
HpyF3I CTNAG 4 cut(s) 10, 137, 204, 215
Hsp92II CATG 1 cut(s) 667
Kpn2I TCCGGA 2 cut(s) 274, 505
Kzo9I GATC 5 cut(s) 116, 221, 484, 496, 625
LmnI GCTCC 2 cut(s) 28, 515
LweI GCATC 2 cut(s) 380, 481
MabI ACCWGGT 1 cut(s) 246
MaeIII GTNAC 3 cut(s) 473, 593, 601
MalI GATC 5 cut(s) 118, 223, 486, 498, 627
MboI GATC 5 cut(s) 116, 221, 484, 496, 625
MboII GAAGA 6 cut(s) 168, 183, 244, 304, 650, 653
MflI RGATCY 1 cut(s) 221
MluCI AATT 1 cut(s) 651
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 6 cut(s) 205, 269, 313, 391, 433, 506
MroI TCCGGA 2 cut(s) 274, 505
MroXI GAANNNNTTC 1 cut(s) 175
MseI TTAA 5 cut(s) 35, 56, 78, 339, 590
MslI CAYNNNNRTG 1 cut(s) 668
MspI CCGG 3 cut(s) 229, 275, 506
MspR9I CCNGG 2 cut(s) 248, 328
MvaI CCWGG 2 cut(s) 248, 328
MwoI GCNNNNNNNGC 2 cut(s) 225, 299
NdeII GATC 5 cut(s) 116, 221, 484, 496, 625
NlaIII CATG 1 cut(s) 667
NmuCI GTSAC 1 cut(s) 601
PaqCI CACCTGC 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 154
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PsiI TTATAA 1 cut(s) 617
Psp6I CCWGG 2 cut(s) 246, 326
PspGI CCWGG 2 cut(s) 246, 326
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 2 cut(s) 8, 425
RsaNI GTAC 2 cut(s) 7, 424
RseI CAYNNNNRTG 1 cut(s) 668
SaqAI TTAA 5 cut(s) 35, 56, 78, 339, 590
Sau3AI GATC 5 cut(s) 116, 221, 484, 496, 625
ScaI AGTACT 1 cut(s) 8
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 248, 328
SetI ASST 8 cut(s) 97, 202, 249, 329, 359, 422, 444, 520
SexAI ACCWGGT 1 cut(s) 246
SfaNI GCATC 2 cut(s) 380, 481
SmiI ATTTAAAT 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 668
Sse9I AATT 1 cut(s) 651
StyD4I CCNGG 2 cut(s) 246, 326
StyI CCWWGG 1 cut(s) 24
SwaI ATTTAAAT 1 cut(s) 57
TaaI ACNGT 2 cut(s) 132, 335
TasI AATT 1 cut(s) 651
TatI WGTACW 2 cut(s) 6, 423
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 5 cut(s) 35, 56, 78, 339, 590
Tru9I TTAA 5 cut(s) 35, 56, 78, 339, 590
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 601
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 2 cut(s) 17, 651
TspGWI ACGGA 3 cut(s) 489, 566, 620
TspRI CASTG 1 cut(s) 295
XapI RAATTY 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 175
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.