MD16G1031300.v1.1

Repressor of RNA polymerase III transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010817
Physical Location & Seq
Forward (+)
2225140 .. 2228622
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1031300.v1.1.491

Sequence Viewer

Length: 675 bp
ATGAAGTTCTTGGAATACACCCCGTTGGAGCGGTTAAATGATTTCTTTAGTGATTTGAATCTCGGAGAGCGAACAATTCAGGGTTGTCTTGAAGCTTACTCGTGCAAACACACCGGATCGGATAAGAAATTGTCTCTCGGTTTGGAGAATGAGATTCTTGATTATCTTGGGAAATCTTCGGACTCTGATTCTTCCTCGCCTGCTGAATTTCTGCTGACCAGATCCAGCCGGAAGACATTGATTTACCTTGTTTTGACACTCTATCACTTGTATCCAGACTATGACTTCAGTGCAGTAAATGCTCATCAGTTTTTCACAGAGGAAAGCTGGGACAGTTTTAAGCAGATTTTTGATACCTATATGTTTGAAGCATCAAAGGAATGGACAGAGACAAATGAGGGCAGTTCCTTGCTGGAAGCCTTGTACAAGGCTTTGGATGAGGTTGTTAGACTAGCGGAATGTGAAGTTTACAGTTACAGTCCGGACTCGGATGCTGATCCGTTTCTTGAGAGAGGATCCATATGGTCGTTCAATTTCTTTTTCTACAATAGGAAACTTAAGCGTGTTGTGAGCTTCCACGTTTGCTGTTTAAGTAACCTGGTGGCTGATGGATTTCTGATGGACAAGTTGCGCTACGATGAAGATGGAGAAATTTTCGACAACATGGACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf1

Protein Analysis

225

Amino Acids

26.04

Weight (kDa)

4.48

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf1 PF09174 26 - 193 1.6e-52 Maf1 regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 31
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 2 cut(s) 31, 455
AclWI GGATC 5 cut(s) 124, 216, 491, 510, 523
AcsI RAATTY 2 cut(s) 206, 651
AcuI CTGAAG 1 cut(s) 271
AfaI GTAC 1 cut(s) 425
AflII CTTAAG 1 cut(s) 557
AgsI TTSAA 4 cut(s) 58, 92, 368, 532
AjnI CCWGG 1 cut(s) 597
AluBI AGCT 3 cut(s) 95, 327, 573
AluI AGCT 3 cut(s) 95, 327, 573
Alw26I GTCTC 2 cut(s) 138, 383
AlwI GGATC 5 cut(s) 124, 216, 491, 510, 523
Aor13HI TCCGGA 1 cut(s) 481
ApoI RAATTY 2 cut(s) 206, 651
AspLEI GCGC 1 cut(s) 633
BamHI GGATCC 1 cut(s) 515
BauI CACGAG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 239
BccI CCATC 3 cut(s) 602, 613, 638
BciT130I CCWGG 1 cut(s) 599
BciVI GTATCC 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 138, 383
BfaI CTAG 1 cut(s) 452
BfrI CTTAAG 1 cut(s) 557
BfuI GTATCC 1 cut(s) 282
Bme1390I CCNGG 1 cut(s) 599
BmiI GGNNCC 1 cut(s) 517
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 2 cut(s) 380, 481
BpiI GAAGAC 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 527
BsaBI GATNNNNATC 2 cut(s) 57, 495
BsaWI WCCGGW 2 cut(s) 113, 481
Bse8I GATNNNNATC 2 cut(s) 57, 495
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 1 cut(s) 599
BseGI GGATG 2 cut(s) 442, 496
BseJI GATNNNNATC 2 cut(s) 57, 495
BseYI CCCAGC 1 cut(s) 327
BsgI GTGCAG 1 cut(s) 312
BsiSI CCGG 3 cut(s) 114, 229, 482
BslFI GGGAC 1 cut(s) 344
BsmAI GTCTC 2 cut(s) 138, 383
BsmFI GGGAC 1 cut(s) 344
Bsp13I TCCGGA 1 cut(s) 481
Bsp1407I TGTACA 1 cut(s) 423
Bsp143I GATC 4 cut(s) 116, 221, 496, 515
BspACI CCGC 2 cut(s) 31, 455
BspEI TCCGGA 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 517
BspPI GGATC 5 cut(s) 124, 216, 491, 510, 523
BspTI CTTAAG 1 cut(s) 557
BsrBI CCGCTC 1 cut(s) 31
BsrGI TGTACA 1 cut(s) 423
BssMI GATC 4 cut(s) 116, 221, 496, 515
BssSI CACGAG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 3 cut(s) 335, 473, 479
BstAFI CTTAAG 1 cut(s) 557
BstAPI GCANNNNNTGC 1 cut(s) 299
BstAUI TGTACA 1 cut(s) 423
BstC8I GCNNGC 1 cut(s) 201
BstF5I GGATG 2 cut(s) 442, 496
BstHHI GCGC 1 cut(s) 633
BstKTI GATC 4 cut(s) 119, 224, 499, 518
BstMAI GTCTC 2 cut(s) 138, 383
BstMBI GATC 4 cut(s) 116, 221, 496, 515
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNI CCWGG 1 cut(s) 599
BstSCI CCNGG 1 cut(s) 597
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 2 cut(s) 221, 515
BstYI RGATCY 2 cut(s) 221, 515
BsuI GTATCC 1 cut(s) 282
BtsCI GGATG 2 cut(s) 442, 496
BtsIMutI CAGTG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 201
CfoI GCGC 1 cut(s) 633
CsiI ACCWGGT 1 cut(s) 597
Csp6I GTAC 1 cut(s) 424
CviAII CATG 1 cut(s) 664
CviJI RGCY 7 cut(s) 95, 228, 327, 419, 431, 573, 605
CviKI_1 RGCY 7 cut(s) 95, 228, 327, 419, 431, 573, 605
CviQI GTAC 1 cut(s) 424
DpnI GATC 4 cut(s) 118, 223, 498, 517
DpnII GATC 4 cut(s) 116, 221, 496, 515
Eco57I CTGAAG 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 597
FaeI CATG 1 cut(s) 667
FaiI YATR 6 cut(s) 282, 360, 362, 521, 523, 665
FaqI GGGAC 1 cut(s) 344
FatI CATG 1 cut(s) 663
FauNDI CATATG 1 cut(s) 521
FokI GGATG 2 cut(s) 449, 503
FspBI CTAG 1 cut(s) 452
GlaI GCGC 1 cut(s) 632
GsaI CCCAGC 1 cut(s) 331
HapII CCGG 3 cut(s) 114, 229, 482
HhaI GCGC 1 cut(s) 633
Hin1II CATG 1 cut(s) 667
Hin6I GCGC 1 cut(s) 631
HinP1I GCGC 1 cut(s) 631
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 5 cut(s) 58, 154, 182, 188, 485
HpaII CCGG 3 cut(s) 114, 229, 482
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 6 cut(s) 65, 121, 181, 187, 490, 618
Hpy188III TCNNGA 5 cut(s) 89, 158, 275, 482, 506
Hpy8I GTNNAC 1 cut(s) 469
HpyCH4III ACNGT 3 cut(s) 335, 473, 479
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 2 cut(s) 105, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpySE526I ACGT 1 cut(s) 579
Hsp92II CATG 1 cut(s) 667
HspAI GCGC 1 cut(s) 631
Kpn2I TCCGGA 1 cut(s) 481
Kzo9I GATC 4 cut(s) 116, 221, 496, 515
LmnI GCTCC 1 cut(s) 28
LweI GCATC 2 cut(s) 380, 481
MabI ACCWGGT 1 cut(s) 597
MaeI CTAG 1 cut(s) 452
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 2 cut(s) 473, 593
MalI GATC 4 cut(s) 118, 223, 498, 517
MbiI CCGCTC 1 cut(s) 31
MboI GATC 4 cut(s) 116, 221, 496, 515
MboII GAAGA 4 cut(s) 168, 183, 244, 653
MflI RGATCY 2 cut(s) 221, 515
MluCI AATT 5 cut(s) 75, 128, 206, 532, 651
MlyI GAGTC 2 cut(s) 176, 479
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 5 cut(s) 205, 313, 391, 433, 506
MroI TCCGGA 1 cut(s) 481
MseI TTAA 4 cut(s) 35, 339, 558, 590
MspCI CTTAAG 1 cut(s) 557
MspI CCGG 3 cut(s) 114, 229, 482
MspR9I CCNGG 1 cut(s) 599
MvaI CCWGG 1 cut(s) 599
MwoI GCNNNNNNNGC 1 cut(s) 299
NdeI CATATG 1 cut(s) 521
NdeII GATC 4 cut(s) 116, 221, 496, 515
NlaIII CATG 1 cut(s) 667
NlaIV GGNNCC 1 cut(s) 517
PfeI GAWTC 3 cut(s) 58, 154, 188
PleI GAGTC 2 cut(s) 176, 479
PpsI GAGTC 2 cut(s) 176, 479
Psp6I CCWGG 1 cut(s) 597
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 1 cut(s) 597
PspN4I GGNNCC 1 cut(s) 517
PsuI RGATCY 2 cut(s) 221, 515
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SaqAI TTAA 4 cut(s) 35, 339, 558, 590
Sau3AI GATC 4 cut(s) 116, 221, 496, 515
SchI GAGTC 2 cut(s) 176, 479
ScrFI CCNGG 1 cut(s) 599
SetI ASST 8 cut(s) 97, 249, 329, 359, 444, 575, 582, 600
SexAI ACCWGGT 1 cut(s) 597
SfaNI GCATC 2 cut(s) 380, 481
SmlI CTYRAG 2 cut(s) 506, 557
SmoI CTYRAG 2 cut(s) 506, 557
Sse9I AATT 5 cut(s) 75, 128, 206, 532, 651
SsiI CCGC 2 cut(s) 31, 455
SspMI CTAG 1 cut(s) 452
StyD4I CCNGG 1 cut(s) 597
TaaI ACNGT 3 cut(s) 335, 473, 479
TaiI ACGT 1 cut(s) 582
TaqI TCGA 1 cut(s) 657
TasI AATT 5 cut(s) 75, 128, 206, 532, 651
TatI WGTACW 1 cut(s) 423
TfiI GAWTC 3 cut(s) 58, 154, 188
Tru1I TTAA 4 cut(s) 35, 339, 558, 590
Tru9I TTAA 4 cut(s) 35, 339, 558, 590
TscAI CASTG 1 cut(s) 295
TspDTI ATGAA 2 cut(s) 17, 654
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 1 cut(s) 295
Vha464I CTTAAG 1 cut(s) 557
XapI RAATTY 2 cut(s) 206, 651
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.