FvH4_4g27520

NIM1-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008703
Physical Location & Seq
Forward (+)
28490142 .. 28490447
306 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27520.t1

Sequence Viewer

Length: 306 bp
ATGGATGCAGAAAAGCGAAAGCGCGACGATGAGGGCAGAATTGACGGGAAGAGAGCGAAACCGGAGGAAGATAACGGCACGGTGGTGACGGAGGAGGAGGTGGAGGAGTTCTTTACCATACTCAGGAGGATACAAGTGGCAGCCAGGCAGTTTGGGAAACCTGACGGAGATGCTGGCCGTAAGTTGACTACGTCCAAGAAATTGAGGCTCTCTTTCGAAGCAAGAGACTTTGAGGAAGAGAAAAGGCCGGATGCGGATTCGGGTTTCGATCTCAATTTGGATCCTGCTGCTTCGGAAGAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

102

Amino Acids

11.49

Weight (kDa)

4.75

Isoelectric Point (pI)

62.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 24
AciI CCGC 1 cut(s) 254
AclWI GGATC 2 cut(s) 275, 288
AcoI YGGCCR 1 cut(s) 175
AfiI CCNNNNNNNGG 1 cut(s) 123
AjnI CCWGG 1 cut(s) 143
AleI CACNNNNGTG 1 cut(s) 83
AluBI AGCT 1 cut(s) 303
AluI AGCT 1 cut(s) 303
Alw26I GTCTC 1 cut(s) 219
AlwI GGATC 2 cut(s) 275, 288
AoxI GGCC 2 cut(s) 175, 245
ApeKI GCWGC 2 cut(s) 140, 287
AspLEI GCGC 1 cut(s) 24
AsuHPI GGTGA 1 cut(s) 97
AsuII TTCGAA 1 cut(s) 216
BamHI GGATCC 1 cut(s) 280
BbvI GCAGC 2 cut(s) 152, 274
BceAI ACGGC 2 cut(s) 91, 162
BciT130I CCWGG 1 cut(s) 145
BciVI GTATCC 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 219
BfuI GTATCC 1 cut(s) 123
BisI GCNGC 2 cut(s) 141, 288
BlsI GCNGC 2 cut(s) 142, 289
Bme1390I CCNGG 1 cut(s) 145
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 2 cut(s) 160, 241
Bpu14I TTCGAA 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 145
BseGI GGATG 2 cut(s) 10, 256
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 136
BseRI GAGGAG 3 cut(s) 107, 110, 119
BseXI GCAGC 2 cut(s) 152, 274
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 177, 247
BsiSI CCGG 2 cut(s) 62, 248
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 219
BsnI GGCC 2 cut(s) 177, 247
Bsp119I TTCGAA 1 cut(s) 216
Bsp143I GATC 2 cut(s) 268, 280
BspACI CCGC 1 cut(s) 254
BspANI GGCC 2 cut(s) 177, 247
BspCNI CTCAG 1 cut(s) 135
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 2 cut(s) 275, 288
BspT104I TTCGAA 1 cut(s) 216
BssMI GATC 2 cut(s) 268, 280
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 2 cut(s) 44, 231
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 175
BstDEI CTNAG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 10, 256
BstFNI CGCG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 2 cut(s) 271, 283
BstMAI GTCTC 1 cut(s) 219
BstMBI GATC 2 cut(s) 268, 280
BstNI CCWGG 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 143
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 152, 274
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuI GTATCC 1 cut(s) 123
BsuRI GGCC 2 cut(s) 177, 247
BtsCI GGATG 2 cut(s) 10, 256
Cac8I GCNNGC 1 cut(s) 175
CfoI GCGC 1 cut(s) 24
CviJI RGCY 5 cut(s) 143, 177, 208, 247, 303
CviKI_1 RGCY 5 cut(s) 143, 177, 208, 247, 303
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 270, 282
DpnII GATC 2 cut(s) 268, 280
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 2 cut(s) 44, 231
EarI CTCTTC 2 cut(s) 44, 231
EcoRII CCWGG 1 cut(s) 143
FaiI YATR 1 cut(s) 119
Fnu4HI GCNGC 2 cut(s) 141, 288
FokI GGATG 2 cut(s) 17, 263
Fsp4HI GCNGC 2 cut(s) 141, 288
GlaI GCGC 1 cut(s) 23
GluI GCNGC 2 cut(s) 141, 288
HaeIII GGCC 2 cut(s) 177, 247
HapII CCGG 2 cut(s) 62, 248
HhaI GCGC 1 cut(s) 24
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HinfI GANTC 1 cut(s) 257
HpaII CCGG 2 cut(s) 62, 248
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 1 cut(s) 295
Hpy188III TCNNGA 1 cut(s) 124
Hpy8I GTNNAC 1 cut(s) 186
Hpy99I CGWCG 1 cut(s) 29
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 1 cut(s) 8
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 191
HspAI GCGC 1 cut(s) 22
Kzo9I GATC 2 cut(s) 268, 280
LpnPI CCDG 8 cut(s) 75, 109, 130, 157, 159, 174, 261, 297
Lsp1109I GCAGC 2 cut(s) 152, 274
LweI GCATC 2 cut(s) 160, 241
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 85
MalI GATC 2 cut(s) 270, 282
MboI GATC 2 cut(s) 268, 280
MboII GAAGA 3 cut(s) 61, 80, 248
MflI RGATCY 1 cut(s) 280
MluCI AATT 3 cut(s) 39, 200, 274
MnlI CCTC 9 cut(s) 25, 58, 85, 88, 91, 97, 120, 198, 226
MslI CAYNNNNRTG 1 cut(s) 83
MspI CCGG 2 cut(s) 62, 248
MspR9I CCNGG 1 cut(s) 145
MvaI CCWGG 1 cut(s) 145
MvnI CGCG 1 cut(s) 24
NdeII GATC 2 cut(s) 268, 280
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 85
NspV TTCGAA 1 cut(s) 216
OliI CACNNNNGTG 1 cut(s) 83
PfeI GAWTC 1 cut(s) 257
PflFI GACNNNGTC 1 cut(s) 190
PkrI GCNGC 2 cut(s) 142, 289
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 1 cut(s) 282
PsuI RGATCY 1 cut(s) 280
PsyI GACNNNGTC 1 cut(s) 190
RseI CAYNNNNRTG 1 cut(s) 83
SatI GCNGC 2 cut(s) 141, 288
Sau3AI GATC 2 cut(s) 268, 280
ScrFI CCNGG 1 cut(s) 145
SetI ASST 4 cut(s) 102, 163, 194, 305
SfaNI GCATC 2 cut(s) 160, 241
SfuI TTCGAA 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 83
Sse9I AATT 3 cut(s) 39, 200, 274
SsiI CCGC 1 cut(s) 254
StyD4I CCNGG 1 cut(s) 143
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 194
TaqI TCGA 2 cut(s) 216, 267
TasI AATT 3 cut(s) 39, 200, 274
TfiI GAWTC 1 cut(s) 257
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 2 cut(s) 140, 287
Tsp45I GTSAC 1 cut(s) 85
TspGWI ACGGA 2 cut(s) 104, 180
Tth111I GACNNNGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.