MD13G1042800.v1.1

NIM1-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008703
Physical Location & Seq
Forward (+)
2987006 .. 2989814
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1042800.v1.1.491

Sequence Viewer

Length: 321 bp
ATGGATGCGGATAAGCGGAAGCGCGACGACGGAGGAGAATTAGAGGGGAAGAGAGCGAGAGGAGTCGAGGAAGATCGTAAAAGAGGTCAGGAGACGGTAACGGAGGAGGAGGTGGAGGAGTTCTTTGCGATACTCAGGCGGATACAGGTGGCAGCCAAGCACTTCCGCAGGACAGCGTCGATGGAATGGAGGCGCTCTTTCGAAGTTGAAGATTTTGAGGAAGATAACGAGTCTCTTATGATGAAAGACCAAAAACAGGAAGAGAAAAAAAGGGAGGCGGGGGCGGATACGGGTTTGGATCTTAACACACATCCCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

107

Amino Acids

12.49

Weight (kDa)

5.0

Isoelectric Point (pI)

70.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 24
AciI CCGC 6 cut(s) 8, 16, 139, 166, 278, 284
AclWI GGATC 1 cut(s) 306
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 1 cut(s) 209
Alw26I GTCTC 2 cut(s) 86, 237
AlwI GGATC 1 cut(s) 306
ApeKI GCWGC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 24, 195
AsuII TTCGAA 1 cut(s) 201
BbvI GCAGC 1 cut(s) 164
BccI CCATC 1 cut(s) 175
BciVI GTATCC 2 cut(s) 135, 280
BcoDI GTCTC 2 cut(s) 86, 237
BfoI RGCGCY 1 cut(s) 196
BfuI GTATCC 2 cut(s) 135, 280
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bpu14I TTCGAA 1 cut(s) 201
Bsc4I CCNNNNNNNGG 1 cut(s) 256
BseGI GGATG 2 cut(s) 10, 310
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 148
BseRI GAGGAG 5 cut(s) 48, 75, 119, 122, 131
BseXI GCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 2 cut(s) 86, 237
BsmBI CGTCTC 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 201
Bsp143I GATC 2 cut(s) 73, 298
BspACI CCGC 6 cut(s) 8, 16, 139, 166, 278, 284
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 1 cut(s) 24
BspPI GGATC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 201
BssMI GATC 2 cut(s) 73, 298
Bst4CI ACNGT 1 cut(s) 97
Bst6I CTCTTC 2 cut(s) 44, 255
BstBI TTCGAA 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 10, 310
BstFNI CGCG 1 cut(s) 24
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 24, 195
BstKTI GATC 2 cut(s) 76, 301
BstMAI GTCTC 2 cut(s) 86, 237
BstMBI GATC 2 cut(s) 73, 298
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuI GTATCC 2 cut(s) 135, 280
BtsCI GGATG 2 cut(s) 10, 310
CfoI GCGC 2 cut(s) 24, 195
CseI GACGC 1 cut(s) 165
CviJI RGCY 1 cut(s) 155
CviKI_1 RGCY 1 cut(s) 155
DdeI CTNAG 1 cut(s) 134
DpnI GATC 2 cut(s) 75, 300
DpnII GATC 2 cut(s) 73, 298
Eam1104I CTCTTC 2 cut(s) 44, 255
EarI CTCTTC 2 cut(s) 44, 255
EciI GGCGGA 2 cut(s) 154, 299
Esp3I CGTCTC 1 cut(s) 86
FaiI YATR 2 cut(s) 239, 319
FauI CCCGC 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 2 cut(s) 17, 297
Fsp4HI GCNGC 1 cut(s) 153
GlaI GCGC 2 cut(s) 23, 194
GluI GCNGC 1 cut(s) 153
HaeII RGCGCY 1 cut(s) 196
HgaI GACGC 1 cut(s) 165
HhaI GCGC 2 cut(s) 24, 195
Hin6I GCGC 2 cut(s) 22, 193
HinP1I GCGC 2 cut(s) 22, 193
HinfI GANTC 2 cut(s) 63, 230
Hpy188III TCNNGA 1 cut(s) 89
Hpy99I CGWCG 3 cut(s) 29, 32, 181
HpyCH4III ACNGT 1 cut(s) 97
HpyF3I CTNAG 1 cut(s) 134
HspAI GCGC 2 cut(s) 22, 193
Kzo9I GATC 2 cut(s) 73, 298
LpnPI CCDG 5 cut(s) 74, 121, 131, 154, 242
Lsp1109I GCAGC 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 97
MalI GATC 2 cut(s) 75, 300
MboI GATC 2 cut(s) 73, 298
MboII GAAGA 5 cut(s) 61, 83, 221, 233, 272
MflI RGATCY 1 cut(s) 298
MluCI AATT 1 cut(s) 38
MlyI GAGTC 2 cut(s) 72, 239
MseI TTAA 1 cut(s) 303
MvnI CGCG 1 cut(s) 24
NdeII GATC 2 cut(s) 73, 298
NspV TTCGAA 1 cut(s) 201
PflFI GACNNNGTC 1 cut(s) 175
PkrI GCNGC 1 cut(s) 154
PleI GAGTC 2 cut(s) 71, 238
PpsI GAGTC 2 cut(s) 71, 238
PsuI RGATCY 1 cut(s) 298
PsyI GACNNNGTC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 2 cut(s) 73, 298
SchI GAGTC 2 cut(s) 72, 239
SetI ASST 3 cut(s) 88, 114, 150
SfuI TTCGAA 1 cut(s) 201
Sse9I AATT 1 cut(s) 38
SsiI CCGC 6 cut(s) 8, 16, 139, 166, 278, 284
TaaI ACNGT 1 cut(s) 97
TaqI TCGA 3 cut(s) 66, 179, 201
TasI AATT 1 cut(s) 38
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 257
TspGWI ACGGA 2 cut(s) 45, 116
Tth111I GACNNNGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.