pycom13g03750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008703
Physical Location & Seq
Forward (+)
2491127 .. 2491498
372 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03750.1

Sequence Viewer

Length: 372 bp
ATGTTTAACGGAAAAGAAATGGAGGCAGAGAAGAGAAAGCAGCCGGACAGCGACGGCATCATCTCAGGGAGGCAAAGGCAAAAGAAGGCTCGGGATTATAAGGGAGACGTCACGGCCGTGAAGGAGGACGAGGTTGACGAGTTCTTCGCTATCCTCGGACGGATACGCCGGGTGTTCAGGCACTTCAAGAATGCTGATGGTGGCGGCAGCCTTAAAGTAGCGGGCGGAGGATCCCGATTGAGGGCAATGCTCGAGCGTGATGAAGAAGCAAACGACGTTCAACTGGGGACCAACATGCAAGATTGTGGTGTGGAGGAGAATATGGGCTTGGATTTGAATGCAGATCCGGATCCGGAATGTGATCCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

13.68

Weight (kDa)

4.88

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 99
AatII GACGTC 1 cut(s) 111
AccIII TCCGGA 2 cut(s) 346, 352
AciI CCGC 3 cut(s) 204, 221, 225
AclWI GGATC 6 cut(s) 225, 238, 338, 344, 356, 357
AcoI YGGCCR 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 240, 241
AgsI TTSAA 3 cut(s) 187, 281, 337
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AleI CACNNNNGTG 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 6 cut(s) 225, 238, 338, 344, 356, 357
Ama87I CYCGRG 2 cut(s) 90, 251
Aor13HI TCCGGA 2 cut(s) 346, 352
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 2 cut(s) 40, 207
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspS9I GGNCC 1 cut(s) 288
AsuC2I CCSGG 1 cut(s) 170
AvaI CYCGRG 2 cut(s) 90, 251
AvaII GGWCC 1 cut(s) 288
BamHI GGATCC 2 cut(s) 230, 349
BbvI GCAGC 2 cut(s) 52, 219
BccI CCATC 1 cut(s) 191
BceAI ACGGC 3 cut(s) 70, 101, 129
BciVI GTATCC 1 cut(s) 156
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 99
BfuI GTATCC 1 cut(s) 156
BisI GCNGC 3 cut(s) 41, 205, 208
BlsI GCNGC 3 cut(s) 42, 206, 209
Bme1390I CCNGG 1 cut(s) 170
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 2 cut(s) 90, 251
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 3 cut(s) 232, 289, 351
BmrFI CCNGG 1 cut(s) 170
BmrI ACTGGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 66
BmuI ACTGGG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 154
BsaWI WCCGGW 2 cut(s) 346, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 241
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 348
BseAI TCCGGA 2 cut(s) 346, 352
BseDI CCNNGG 1 cut(s) 154
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 2 cut(s) 240, 241
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 329
BseX3I CGGCCG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 52, 219
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 116
BsiEI CGRYCG 1 cut(s) 117
BsiHKCI CYCGRG 2 cut(s) 90, 251
BsiSI CCGG 4 cut(s) 44, 169, 347, 353
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 240, 241
BsmAI GTCTC 1 cut(s) 99
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 1 cut(s) 301
BsmI GAATGC 2 cut(s) 196, 343
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 2 cut(s) 90, 251
Bsp13I TCCGGA 2 cut(s) 346, 352
Bsp143I GATC 4 cut(s) 230, 343, 349, 361
BspACI CCGC 3 cut(s) 204, 221, 225
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 1 cut(s) 77
BspEI TCCGGA 2 cut(s) 346, 352
BspLI GGNNCC 3 cut(s) 232, 289, 351
BspPI GGATC 6 cut(s) 225, 238, 338, 344, 356, 357
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 4 cut(s) 230, 343, 349, 361
BssNI GRCGYC 1 cut(s) 108
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 1 cut(s) 64
BstKTI GATC 4 cut(s) 233, 346, 352, 364
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 4 cut(s) 230, 343, 349, 361
BstMCI CGRYCG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 52, 219
BstX2I RGATCY 3 cut(s) 230, 343, 349
BstYI RGATCY 3 cut(s) 230, 343, 349
BstZI CGGCCG 1 cut(s) 114
BsuI GTATCC 1 cut(s) 156
BsuRI GGCC 1 cut(s) 116
Cac8I GCNNGC 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 288
CviAII CATG 1 cut(s) 295
CviJI RGCY 5 cut(s) 43, 89, 116, 210, 327
CviKI_1 RGCY 5 cut(s) 43, 89, 116, 210, 327
DdeI CTNAG 1 cut(s) 64
DpnI GATC 4 cut(s) 232, 345, 351, 363
DpnII GATC 4 cut(s) 230, 343, 349, 361
EaeI YGGCCR 1 cut(s) 114
EagI CGGCCG 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 240
EclXI CGGCCG 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 288
Eco52I CGGCCG 1 cut(s) 114
Eco88I CYCGRG 2 cut(s) 90, 251
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 1 cut(s) 298
FaiI YATR 3 cut(s) 99, 296, 323
FaqI GGGAC 1 cut(s) 301
FatI CATG 1 cut(s) 294
FauI CCCGC 1 cut(s) 214
Fnu4HI GCNGC 3 cut(s) 41, 205, 208
Fsp4HI GCNGC 3 cut(s) 41, 205, 208
GluI GCNGC 3 cut(s) 41, 205, 208
HaeIII GGCC 1 cut(s) 116
HapII CCGG 4 cut(s) 44, 169, 347, 353
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 1 cut(s) 298
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HpaII CCGG 4 cut(s) 44, 169, 347, 353
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 158
Hpy188III TCNNGA 5 cut(s) 92, 187, 234, 347, 353
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 2 cut(s) 56, 278
HpyAV CCTTC 2 cut(s) 79, 115
HpyCH4IV ACGT 2 cut(s) 108, 276
HpyCH4V TGCA 2 cut(s) 298, 341
HpyF3I CTNAG 1 cut(s) 64
HpySE526I ACGT 2 cut(s) 108, 276
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 1 cut(s) 298
Kpn2I TCCGGA 2 cut(s) 346, 352
Kzo9I GATC 4 cut(s) 230, 343, 349, 361
LpnPI CCDG 7 cut(s) 51, 57, 163, 182, 269, 360, 366
Lsp1109I GCAGC 2 cut(s) 52, 219
LweI GCATC 1 cut(s) 66
MaeII ACGT 2 cut(s) 108, 276
MaeIII GTNAC 1 cut(s) 109
MalI GATC 4 cut(s) 232, 345, 351, 363
MboI GATC 4 cut(s) 230, 343, 349, 361
MboII GAAGA 3 cut(s) 43, 136, 275
MflI RGATCY 3 cut(s) 230, 343, 349
MnlI CCTC 8 cut(s) 16, 63, 118, 124, 164, 221, 234, 307
MroI TCCGGA 2 cut(s) 346, 352
MseI TTAA 2 cut(s) 6, 213
MslI CAYNNNNRTG 1 cut(s) 116
MspI CCGG 4 cut(s) 44, 169, 347, 353
MspR9I CCNGG 1 cut(s) 170
Mva1269I GAATGC 2 cut(s) 196, 343
NciI CCSGG 1 cut(s) 170
NdeII GATC 4 cut(s) 230, 343, 349, 361
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 3 cut(s) 232, 289, 351
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 298
OliI CACNNNNGTG 1 cut(s) 116
PaeR7I CTCGAG 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 135
PctI GAATGC 2 cut(s) 196, 343
PkrI GCNGC 3 cut(s) 42, 206, 209
PsiI TTATAA 1 cut(s) 99
PspN4I GGNNCC 3 cut(s) 232, 289, 351
PspPI GGNCC 1 cut(s) 288
PspXI VCTCGAGB 1 cut(s) 251
PsuI RGATCY 3 cut(s) 230, 343, 349
RseI CAYNNNNRTG 1 cut(s) 116
SaqAI TTAA 2 cut(s) 6, 213
SatI GCNGC 3 cut(s) 41, 205, 208
Sau3AI GATC 4 cut(s) 230, 343, 349, 361
Sau96I GGNCC 1 cut(s) 288
ScrFI CCNGG 1 cut(s) 170
SetI ASST 3 cut(s) 111, 135, 279
SfaNI GCATC 1 cut(s) 66
Sfr274I CTCGAG 1 cut(s) 251
SinI GGWCC 1 cut(s) 288
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SsiI CCGC 3 cut(s) 204, 221, 225
StyD4I CCNGG 1 cut(s) 168
TaiI ACGT 2 cut(s) 111, 279
TaqI TCGA 1 cut(s) 252
TauI GCSGC 1 cut(s) 207
Tru1I TTAA 2 cut(s) 6, 213
Tru9I TTAA 2 cut(s) 6, 213
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 2 cut(s) 40, 207
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 276
TspGWI ACGGA 2 cut(s) 24, 175
VpaK11BI GGWCC 1 cut(s) 288
XceI RCATGY 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 251
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.