Rorug04G0284400

NIM1-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008703
Physical Location & Seq
Forward (+)
45749744 .. 45755334
5591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0284400.1

Sequence Viewer

Length: 2340 bp
ATGAGTCACGACAATCTCAAACTAATATTCGAATCTGTAACTTCCATAATATCGACTCATTACGTTGAAGCAACCAGAGACTTTTTAAGTCTGTGCACACTTCTGGGAGCTTCCCCACATTCCTTGTTCATATCTCCTCAACGTTCTTCTTCTTCACTTGGGACCTCGCTCTTCAATGGACACTACTACTCACTAGCTAACAGAGCTGGAGCTATTGAAAAAATCCAAATGGTCGAAAGTGATCTCGACCACCTCAAGCAAGAAATGTCCCAGGTCACCAGCCACCGCCGAATCGCCAGCTCCGGTACTCCGCAGCCCAAAACGAGCGGCAGTGCCAGTCGGCTGCCGTCGACTTTTAACTTGAGTGATGCTCAGTATCTCAACATATTGCAGTCGATTGGGCAGTCTGTGCATGCAATGGATCGTAGTGGTCGTATAATCTACTGGAACAGAAGTGCTGAAAAACTGTATGGTTATTCTCCGGAAGAGGCCCTTGGACAGGTTTCCATTAAGCTCATAGTAGATTCCCGGGACTTTGGTGTAGCATATAATATAGTCCACCGTGTCATCCACGGGGAGAGTTGGACTGGGCAGTTCCCTGTAAAGCACAAATCGGGGGCGAGGTTTTCAGTCATTACAACAAATACTCCATTCTATGATGATGCTGGCAGTTTAATTGGGGTTGTTGCTACATCAACTGATTCTCAGCCCTTTTATGAAATGAGGGTTCCAATGTCAGCTGAGAAGCAACCACAACAAGATCTAAGCTTCAGCAGTCGTGGGGCTAGTGTTACAACTAAATTCGGTGCTGATCCTCAGCAGCCTCTGCGAAAAGCGGTTTTGTCAAAACTAACAGACTTGGCATCGAAGATGAGTTTCAAAGTCAAGTCAAAAATTCAGGCAAGAGAAAACAACATGGATCCCAAAGGTGACAGCGAAGATAGTCATCATCCGTTTCATGGTTCAGATTCTGTCACATCTGCCAATAGGGAGGATGGGAATGCAAGTGGGACTAGCACAGCTACAGGAACTATACCCCCAGCAACTCCTTTTGGTGTATTTAACCATGTTGAGAGCAAGCCTCTAGGAGAACCCTCCATAGACTCTGCCAGTGAGAGCAAAGAAAAATCTTCAATTCACAAGCTAATTGCCTCAAAGGCAGAAGCATGGATTAGTAAGCAGGAGATATCATGGCCTTGGAAAGGAAATGAGCGAGATGGGTCAGTGGCTAGGGCCAGTGGTTTTGTTTGGCCCTGGTTTCATAATGATCAAGTCAATTTTGCCATTCCTCAGAAGAGTTATTGTGTCTCAGAACCAGAAATTCAGGTGAGCGAAAGTAATCGGCCAACAAATAATAATGATGAGACTCAAGGATCCTGGACCTCTCCATTGGATATTAACAGCATAAGCCGTGCCATCAGCCTTAGAAGGACCAGTAGTGCTTTGCCTACGGTGGATGTGGACACTGATTGCTTGGATTATGAAATCTTATGGGAAGACATGATAATTGGAAGACAGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTCAGTGGGATGGATCAGATGTTGCCGTTAAGGTATTCTCCAAGTTAGAATATCCGGATGATTTGTTACTTTCCTTCAAACAAGAGGTATCTCTAATGAAAAGGCTTCGACATCCAAATGTTTTGCTTTTCATGGGAGCGGTGACTTCACCTGAGCGTCTCTGCATTGTCACAGAGTTCCTCCCACGTGGAAGTTTGTATCAAATACTACAGAGGAACCCATCAAAACTAGACTGGAGACGACGTATTAATATGGCTATGGATATAGCATGGGGCATGAATTATCTTCACCATTTCAACCCACCAATCATTCATCGAGACTTAAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGCGATTTTGGTCTTTCACGTTTTAAGCATGAAACTTTTCTTATAAACAAGAGTGGCAGGGGAACGCCTCAATGGATGTCACCAGAATTTCTACGTAGTGAACCCTCGGATGAGAAGTCTGACATATATAGCTACGGGGTGATATTGTGGGAGCTTGCTACTGAGAAGATTCCTTGGGATAATCTAAACCCAATGCAGGTGATTGGAGCTGTTGGATTCATGAACCAAAGACTGGAGATCCCAAAAGATGTGGACCCAAAGTGGGCTTCTATAATGGAGAGTTGCTGGCACAGTGATCCAGCTAGCCGGCCAACATTTCTAGAACTGCTCGAAAAGCTTAGAGTGTTGCATAGACAATATAATCTCCAGTTCAAGGCAGCGCGTTCCGCCTCTAGTGAGAGCACCCAAAAGGAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

779

Amino Acids

87.2

Weight (kDa)

6.68

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 125 - 231 3.7e-11 PAS fold
PAS_4 PF08448 131 - 235 4.2e-09 PAS fold
PAS_9 PF13426 141 - 234 5.3e-08 PAS domain
PAS_3 PF08447 146 - 231 1.9e-08 PAS fold
PK_Tyr_Ser-Thr PF07714 503 - 753 1.9e-66 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 504 - 751 1e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1964
AarI CACCTGC 1 cut(s) 2107
Acc36I ACCTGC 1 cut(s) 2107
AccB7I CCANNNNNTGG 1 cut(s) 2152
AccBSI CCGCTC 2 cut(s) 327, 1691
AccI GTMKAC 2 cut(s) 350, 1545
AccII CGCG 1 cut(s) 2302
AccIII TCCGGA 2 cut(s) 481, 1606
AciI CCGC 6 cut(s) 286, 311, 327, 836, 1691, 2307
AclI AACGTT 1 cut(s) 142
AclWI GGATC 9 cut(s) 429, 806, 914, 927, 1368, 1381, 1573, 2152, 2210
AcoI YGGCCR 2 cut(s) 1343, 2228
AcsI RAATTY 4 cut(s) 800, 894, 1320, 2006
AcuI CTGAAG 1 cut(s) 754
AcvI CACGTG 1 cut(s) 1739
AfaI GTAC 1 cut(s) 307
AfiI CCNNNNNNNGG 7 cut(s) 499, 959, 1202, 2116, 2152, 2182, 2293
AgsI TTSAA 9 cut(s) 68, 175, 218, 880, 1134, 1630, 1849, 1884, 2293
AhdI GACNNNNNGTC 1 cut(s) 1876
AjnI CCWGG 3 cut(s) 270, 1253, 1376
AjuI GAANNNNNNNTTGG 4 cut(s) 477, 509, 2170, 2202
Alw21I GWGCWC 2 cut(s) 98, 2324
Alw26I GTCTC 6 cut(s) 72, 1312, 1358, 1715, 1783, 1863
Alw44I GTGCAC 1 cut(s) 94
AlwI GGATC 9 cut(s) 429, 806, 914, 927, 1368, 1381, 1573, 2152, 2210
AlwNI CAGNNNCTG 2 cut(s) 826, 971
Ama87I CYCGRG 1 cut(s) 528
Aor13HI TCCGGA 2 cut(s) 481, 1606
AoxI GGCC 6 cut(s) 489, 1193, 1233, 1250, 1343, 2228
ApaLI GTGCAC 1 cut(s) 94
ApeKI GCWGC 4 cut(s) 313, 343, 820, 2297
ApoI RAATTY 4 cut(s) 800, 894, 1320, 2006
AseI ATTAAT 1 cut(s) 1800
Asp700I GAANNNNTTC 1 cut(s) 1956
AspLEI GCGC 1 cut(s) 2302
AspS9I GGNCC 7 cut(s) 162, 490, 1233, 1251, 1380, 1431, 2173
AsuC2I CCSGG 2 cut(s) 529, 530
AsuHPI GGTGA 9 cut(s) 268, 941, 1339, 1692, 1705, 1832, 1992, 2071, 2131
AsuII TTCGAA 1 cut(s) 30
AsuNHI GCTAGC 1 cut(s) 2222
AvaI CYCGRG 1 cut(s) 528
AvaII GGWCC 4 cut(s) 162, 1380, 1431, 2173
BaeGI GKGCMC 1 cut(s) 98
BamHI GGATCC 2 cut(s) 919, 1373
BbrPI CACGTG 1 cut(s) 1739
BbsI GAAGAC 3 cut(s) 1503, 1519, 1872
Bbv12I GWGCWC 2 cut(s) 98, 2324
BbvCI CCTCAGC 1 cut(s) 816
BbvI GCAGC 4 cut(s) 325, 330, 832, 2309
BccI CCATC 5 cut(s) 989, 1211, 1424, 1556, 1780
BceAI ACGGC 3 cut(s) 331, 1395, 1562
BciT130I CCWGG 3 cut(s) 272, 1255, 1378
BclI TGATCA 1 cut(s) 1267
BcnI CCSGG 2 cut(s) 529, 530
BcoDI GTCTC 6 cut(s) 72, 1312, 1358, 1715, 1783, 1863
BfmI CTRYAG 2 cut(s) 1023, 1760
BfuAI ACCTGC 1 cut(s) 2107
BglI GCCNNNNNGGC 1 cut(s) 1157
BglII AGATCT 1 cut(s) 760
BisI GCNGC 5 cut(s) 314, 328, 344, 821, 2298
BlsI GCNGC 5 cut(s) 315, 329, 345, 822, 2299
Bme1390I CCNGG 5 cut(s) 272, 529, 530, 1255, 1378
Bme18I GGWCC 4 cut(s) 162, 1380, 1431, 2173
BmeRI GACNNNNNGTC 1 cut(s) 1876
BmeT110I CYCGRG 1 cut(s) 528
BmgT120I GGNCC 7 cut(s) 162, 490, 1233, 1251, 1380, 1431, 2173
BmiI GGNNCC 6 cut(s) 163, 729, 921, 1375, 1769, 2175
BmrFI CCNGG 5 cut(s) 272, 529, 530, 1255, 1378
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 3 cut(s) 358, 652, 872
BmtI GCTAGC 1 cut(s) 2226
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 3 cut(s) 1503, 1519, 1872
BplI GAGNNNNNCTC 4 cut(s) 355, 387, 1066, 1098
BpmI CTGGAG 4 cut(s) 228, 1807, 2174, 2270
Bpu10I CCTNAGC 2 cut(s) 816, 1704
Bpu14I TTCGAA 1 cut(s) 30
BpuEI CTTGAG 3 cut(s) 239, 382, 1353
BpuMI CCSGG 2 cut(s) 529, 530
BsaAI YACGTR 2 cut(s) 1739, 2015
BsaBI GATNNNNATC 1 cut(s) 945
BsaJI CCNNGG 9 cut(s) 270, 493, 528, 571, 1196, 1253, 1549, 2025, 2093
BsaWI WCCGGW 3 cut(s) 302, 481, 1606
BsaXI ACNNNNNCTCC 4 cut(s) 631, 661, 1780, 1810
Bsc4I CCNNNNNNNGG 7 cut(s) 499, 959, 1202, 2116, 2152, 2182, 2293
Bse118I RCCGGY 1 cut(s) 2226
Bse3DI GCAATG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 945
BseAI TCCGGA 2 cut(s) 481, 1606
BseBI CCWGG 3 cut(s) 272, 1255, 1378
BseDI CCNNGG 9 cut(s) 270, 493, 528, 571, 1196, 1253, 1549, 2025, 2093
BseGI GGATG 9 cut(s) 567, 949, 1000, 1462, 1567, 1615, 1663, 2001, 2035
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 7 cut(s) 499, 959, 1202, 2116, 2152, 2182, 2293
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 8 cut(s) 386, 719, 732, 830, 1304, 1323, 1695, 2073
BseRI GAGGAG 1 cut(s) 126
BseSI GKGCMC 1 cut(s) 98
BseXI GCAGC 4 cut(s) 325, 330, 832, 2309
BseYI CCCAGC 1 cut(s) 1039
Bsh1236I CGCG 1 cut(s) 2302
BshFI GGCC 6 cut(s) 491, 1195, 1235, 1252, 1345, 2230
BsiHKAI GWGCWC 2 cut(s) 98, 2324
BsiHKCI CYCGRG 1 cut(s) 528
BsiSI CCGG 5 cut(s) 303, 482, 529, 1607, 2227
BslFI GGGAC 4 cut(s) 175, 253, 545, 1024
BslI CCNNNNNNNGG 7 cut(s) 499, 959, 1202, 2116, 2152, 2182, 2293
BsmAI GTCTC 6 cut(s) 72, 1312, 1358, 1715, 1783, 1863
BsmBI CGTCTC 2 cut(s) 1715, 1783
BsmFI GGGAC 4 cut(s) 175, 253, 545, 1024
BsmI GAATGC 1 cut(s) 1006
BsnI GGCC 6 cut(s) 491, 1195, 1235, 1252, 1345, 2230
BsoBI CYCGRG 1 cut(s) 528
Bsp119I TTCGAA 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 98, 2324
Bsp13I TCCGGA 2 cut(s) 481, 1606
Bsp19I CCATGG 1 cut(s) 1549
BspACI CCGC 6 cut(s) 286, 311, 327, 836, 1691, 2307
BspANI GGCC 6 cut(s) 491, 1195, 1235, 1252, 1345, 2230
BspCNI CTCAG 8 cut(s) 385, 718, 733, 829, 1303, 1322, 1696, 2074
BspEI TCCGGA 2 cut(s) 481, 1606
BspFNI CGCG 1 cut(s) 2302
BspHI TCATGA 1 cut(s) 2139
BspLI GGNNCC 6 cut(s) 163, 729, 921, 1375, 1769, 2175
BspMI ACCTGC 1 cut(s) 2107
BspOI GCTAGC 1 cut(s) 2226
BspPI GGATC 9 cut(s) 429, 806, 914, 927, 1368, 1381, 1573, 2152, 2210
BspQI GCTCTTC 1 cut(s) 176
BspT104I TTCGAA 1 cut(s) 30
BsrBI CCGCTC 2 cut(s) 327, 1691
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 2226
BssAI RCCGGY 1 cut(s) 2226
BssECI CCNNGG 9 cut(s) 270, 493, 528, 571, 1196, 1253, 1549, 2025, 2093
BssNAI GTATAC 1 cut(s) 1546
BssT1I CCWWGG 4 cut(s) 493, 1196, 1549, 2093
Bst1107I GTATAC 1 cut(s) 1546
Bst2UI CCWGG 3 cut(s) 272, 1255, 1378
Bst4CI ACNGT 6 cut(s) 468, 563, 1453, 1543, 1912, 2213
Bst6I CTCTTC 3 cut(s) 176, 480, 1289
BstAPI GCANNNNNTGC 2 cut(s) 409, 826
BstBAI YACGTR 2 cut(s) 1739, 2015
BstBI TTCGAA 1 cut(s) 30
BstC8I GCNNGC 8 cut(s) 298, 414, 667, 1079, 2076, 2207, 2224, 2228
BstDSI CCRYGG 2 cut(s) 571, 1549
BstEII GGTNACC 1 cut(s) 274
BstENI CCTNNNNNAGG 2 cut(s) 497, 1200
BstF5I GGATG 9 cut(s) 567, 949, 1000, 1462, 1567, 1615, 1663, 2001, 2035
BstFNI CGCG 1 cut(s) 2302
BstHHI GCGC 1 cut(s) 2302
BstMAI GTCTC 6 cut(s) 72, 1312, 1358, 1715, 1783, 1863
BstMWI GCNNNNNNNGC 6 cut(s) 203, 409, 826, 1157, 2254, 2306
BstNI CCWGG 3 cut(s) 272, 1255, 1378
BstNSI RCATGY 1 cut(s) 416
BstPI GGTNACC 1 cut(s) 274
BstSCI CCNGG 5 cut(s) 270, 527, 528, 1253, 1376
BstSFI CTRYAG 2 cut(s) 1023, 1760
BstSLI GKGCMC 1 cut(s) 98
BstSNI TACGTA 1 cut(s) 2015
BstUI CGCG 1 cut(s) 2302
BstV1I GCAGC 4 cut(s) 325, 330, 832, 2309
BstV2I GAAGAC 3 cut(s) 1503, 1519, 1872
BstX2I RGATCY 4 cut(s) 760, 919, 1373, 2157
BstYI RGATCY 4 cut(s) 760, 919, 1373, 2157
BstZ17I GTATAC 1 cut(s) 1546
BsuRI GGCC 6 cut(s) 491, 1195, 1235, 1252, 1345, 2230
BtgI CCRYGG 2 cut(s) 571, 1549
BtsCI GGATG 9 cut(s) 567, 949, 1000, 1462, 1567, 1615, 1663, 2001, 2035
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 7 cut(s) 337, 1117, 1230, 1243, 1464, 1562, 2218
BveI ACCTGC 1 cut(s) 2107
Cac8I GCNNGC 8 cut(s) 298, 414, 667, 1079, 2076, 2207, 2224, 2228
CaiI CAGNNNCTG 2 cut(s) 826, 971
CciI TCATGA 1 cut(s) 2139
CfoI GCGC 1 cut(s) 2302
Cfr10I RCCGGY 1 cut(s) 2226
Cfr13I GGNCC 7 cut(s) 162, 490, 1233, 1251, 1380, 1431, 2173
Cfr9I CCCGGG 1 cut(s) 528
CseI GACGC 1 cut(s) 1697
Csp6I GTAC 1 cut(s) 306
CspCI CAANNNNNGTGG 4 cut(s) 105, 140, 2151, 2186
CviQI GTAC 1 cut(s) 306
DriI GACNNNNNGTC 1 cut(s) 1876
EaeI YGGCCR 2 cut(s) 1343, 2228
Eam1104I CTCTTC 3 cut(s) 176, 480, 1289
Eam1105I GACNNNNNGTC 1 cut(s) 1876
EarI CTCTTC 3 cut(s) 176, 480, 1289
EciI GGCGGA 1 cut(s) 2296
Eco105I TACGTA 1 cut(s) 2015
Eco130I CCWWGG 4 cut(s) 493, 1196, 1549, 2093
Eco32I GATATC 1 cut(s) 1188
Eco47I GGWCC 4 cut(s) 162, 1380, 1431, 2173
Eco57I CTGAAG 1 cut(s) 754
Eco72I CACGTG 1 cut(s) 1739
Eco88I CYCGRG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 274
EcoNI CCTNNNNNAGG 2 cut(s) 497, 1200
EcoO109I RGGNCCY 2 cut(s) 162, 490
EcoO65I GGTNACC 1 cut(s) 274
EcoRII CCWGG 3 cut(s) 270, 1253, 1376
EcoRV GATATC 1 cut(s) 1188
EcoT14I CCWWGG 4 cut(s) 493, 1196, 1549, 2093
ErhI CCWWGG 4 cut(s) 493, 1196, 1549, 2093
Esp3I CGTCTC 2 cut(s) 1715, 1783
FalI AAGNNNNNCTT 2 cut(s) 477, 509
FaqI GGGAC 4 cut(s) 175, 253, 545, 1024
FbaI TGATCA 1 cut(s) 1267
FblI GTMKAC 2 cut(s) 350, 1545
Fnu4HI GCNGC 5 cut(s) 314, 328, 344, 821, 2298
FokI GGATG 9 cut(s) 554, 936, 1007, 1469, 1574, 1622, 1650, 2008, 2042
Fsp4HI GCNGC 5 cut(s) 314, 328, 344, 821, 2298
GlaI GCGC 1 cut(s) 2301
GluI GCNGC 5 cut(s) 314, 328, 344, 821, 2298
GsaI CCCAGC 1 cut(s) 1043
GsuI CTGGAG 4 cut(s) 228, 1807, 2174, 2270
HaeIII GGCC 6 cut(s) 491, 1195, 1235, 1252, 1345, 2230
HapII CCGG 5 cut(s) 303, 482, 529, 1607, 2227
HgaI GACGC 1 cut(s) 1697
HhaI GCGC 1 cut(s) 2302
Hin6I GCGC 1 cut(s) 2300
HinP1I GCGC 1 cut(s) 2300
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HindIII AAGCTT 2 cut(s) 766, 2255
HpaII CCGG 5 cut(s) 303, 482, 529, 1607, 2227
HphI GGTGA 9 cut(s) 268, 941, 1339, 1692, 1705, 1832, 1992, 2071, 2131
Hpy166II GTNNAC 7 cut(s) 96, 351, 559, 1462, 1546, 2021, 2173
Hpy188I TCNGA 6 cut(s) 967, 1293, 1312, 1570, 2029, 2041
Hpy188III TCNNGA 7 cut(s) 8, 245, 482, 1607, 1868, 2140, 2240
Hpy8I GTNNAC 7 cut(s) 96, 351, 559, 1462, 1546, 2021, 2173
Hpy99I CGWCG 2 cut(s) 352, 1797
HpyAV CCTTC 2 cut(s) 1422, 1636
HpyCH4III ACNGT 6 cut(s) 468, 563, 1453, 1543, 1912, 2213
HpyCH4IV ACGT 6 cut(s) 63, 142, 1738, 1795, 1939, 2014
HpyCH4V TGCA 8 cut(s) 96, 391, 412, 416, 1004, 1716, 2116, 2269
HpyF10VI GCNNNNNNNGC 6 cut(s) 203, 409, 826, 1157, 2254, 2306
HpySE526I ACGT 6 cut(s) 63, 142, 1738, 1795, 1939, 2014
HspAI GCGC 1 cut(s) 2300
Kpn2I TCCGGA 2 cut(s) 481, 1606
KroI GCCGGC 1 cut(s) 2226
KroNI GCCGGC 1 cut(s) 2228
Ksp22I TGATCA 1 cut(s) 1267
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 6 cut(s) 107, 209, 305, 1688, 2071, 2126
Lsp1109I GCAGC 4 cut(s) 325, 330, 832, 2309
LweI GCATC 3 cut(s) 358, 652, 872
MaeII ACGT 6 cut(s) 63, 142, 1738, 1795, 1939, 2014
MbiI CCGCTC 2 cut(s) 327, 1691
MflI RGATCY 4 cut(s) 760, 919, 1373, 2157
MhlI GDGCHC 2 cut(s) 98, 2324
MlyI GAGTC 4 cut(s) 13, 49, 1097, 1360
MmeI TCCRAC 2 cut(s) 563, 2113
MroI TCCGGA 2 cut(s) 481, 1606
MroNI GCCGGC 1 cut(s) 2226
MroXI GAANNNNTTC 1 cut(s) 1956
MslI CAYNNNNRTG 1 cut(s) 1668
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 5 cut(s) 303, 482, 529, 1607, 2227
MspR9I CCNGG 5 cut(s) 272, 529, 530, 1255, 1378
Mva1269I GAATGC 1 cut(s) 1006
MvaI CCWGG 3 cut(s) 272, 1255, 1378
MvnI CGCG 1 cut(s) 2302
MwoI GCNNNNNNNGC 6 cut(s) 203, 409, 826, 1157, 2254, 2306
NaeI GCCGGC 1 cut(s) 2228
NciI CCSGG 2 cut(s) 529, 530
NcoI CCATGG 1 cut(s) 1549
NgoMIV GCCGGC 1 cut(s) 2226
NheI GCTAGC 1 cut(s) 2222
NlaIV GGNNCC 6 cut(s) 163, 729, 921, 1375, 1769, 2175
NmuCI GTSAC 7 cut(s) 5, 274, 929, 973, 1693, 1720, 1998
NspI RCATGY 1 cut(s) 416
NspV TTCGAA 1 cut(s) 30
PaeI GCATGC 1 cut(s) 416
PagI TCATGA 1 cut(s) 2139
PaqCI CACCTGC 1 cut(s) 2107
PciSI GCTCTTC 1 cut(s) 176
PcsI WCGNNNNNNNCGW 1 cut(s) 430
PctI GAATGC 1 cut(s) 1006
PdiI GCCGGC 1 cut(s) 2228
PdmI GAANNNNTTC 1 cut(s) 1956
PfeI GAWTC 7 cut(s) 32, 291, 524, 701, 968, 2089, 2136
PflMI CCANNNNNTGG 1 cut(s) 2152
PfoI TCCNGGA 1 cut(s) 1376
PkrI GCNGC 5 cut(s) 315, 329, 345, 822, 2299
PleI GAGTC 4 cut(s) 12, 49, 1097, 1360
PmaCI CACGTG 1 cut(s) 1739
PmlI CACGTG 1 cut(s) 1739
PpsI GAGTC 4 cut(s) 12, 49, 1097, 1360
Ppu21I YACGTR 2 cut(s) 1739, 2015
PpuMI RGGWCCY 1 cut(s) 162
PshBI ATTAAT 1 cut(s) 1800
PsiI TTATAA 1 cut(s) 1964
Psp1406I AACGTT 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 3 cut(s) 270, 1253, 1376
PspCI CACGTG 1 cut(s) 1739
PspEI GGTNACC 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 1039
PspGI CCWGG 3 cut(s) 270, 1253, 1376
PspN4I GGNNCC 6 cut(s) 163, 729, 921, 1375, 1769, 2175
PspPI GGNCC 7 cut(s) 162, 490, 1233, 1251, 1380, 1431, 2173
PspPPI RGGWCCY 1 cut(s) 162
PstNI CAGNNNCTG 2 cut(s) 826, 971
PsuI RGATCY 4 cut(s) 760, 919, 1373, 2157
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 1 cut(s) 307
RsaNI GTAC 1 cut(s) 306
RseI CAYNNNNRTG 1 cut(s) 1668
SalI GTCGAC 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 176
SatI GCNGC 5 cut(s) 314, 328, 344, 821, 2298
Sau96I GGNCC 7 cut(s) 162, 490, 1233, 1251, 1380, 1431, 2173
SchI GAGTC 4 cut(s) 13, 49, 1097, 1360
ScrFI CCNGG 5 cut(s) 272, 529, 530, 1255, 1378
SduI GDGCHC 2 cut(s) 98, 2324
SfaNI GCATC 3 cut(s) 358, 652, 872
SfcI CTRYAG 2 cut(s) 1023, 1760
SfuI TTCGAA 1 cut(s) 30
SinI GGWCC 4 cut(s) 162, 1380, 1431, 2173
SmaI CCCGGG 1 cut(s) 530
SmiMI CAYNNNNRTG 1 cut(s) 1668
SmlI CTYRAG 3 cut(s) 254, 361, 1368
SmoI CTYRAG 3 cut(s) 254, 361, 1368
SnaBI TACGTA 1 cut(s) 2015
SphI GCATGC 1 cut(s) 416
SsiI CCGC 6 cut(s) 286, 311, 327, 836, 1691, 2307
SspI AATATT 1 cut(s) 27
StyD4I CCNGG 5 cut(s) 270, 527, 528, 1253, 1376
StyI CCWWGG 4 cut(s) 493, 1196, 1549, 2093
TaaI ACNGT 6 cut(s) 468, 563, 1453, 1543, 1912, 2213
TaiI ACGT 6 cut(s) 66, 145, 1741, 1798, 1942, 2017
TauI GCSGC 1 cut(s) 330
TfiI GAWTC 7 cut(s) 32, 291, 524, 701, 968, 2089, 2136
TscAI CASTG 7 cut(s) 337, 1117, 1230, 1243, 1471, 1562, 2218
TseFI GTSAC 7 cut(s) 5, 274, 929, 973, 1693, 1720, 1998
TseI GCWGC 4 cut(s) 313, 343, 820, 2297
Tsp45I GTSAC 7 cut(s) 5, 274, 929, 973, 1693, 1720, 1998
TspGWI ACGGA 1 cut(s) 942
TspMI CCCGGG 1 cut(s) 528
TspRI CASTG 7 cut(s) 337, 1117, 1230, 1243, 1471, 1562, 2218
Van91I CCANNNNNTGG 1 cut(s) 2152
VneI GTGCAC 1 cut(s) 94
VpaK11BI GGWCC 4 cut(s) 162, 1380, 1431, 2173
VspI ATTAAT 1 cut(s) 1800
XagI CCTNNNNNAGG 2 cut(s) 497, 1200
XapI RAATTY 4 cut(s) 800, 894, 1320, 2006
XbaI TCTAGA 1 cut(s) 2239
XceI RCATGY 1 cut(s) 416
XmaI CCCGGG 1 cut(s) 528
XmiI GTMKAC 2 cut(s) 350, 1545
XmnI GAANNNNTTC 1 cut(s) 1956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.