Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0008703

Physical Location & Seq

Genomic Coordinates Forward (+)

45741374 .. 45747996

Gene Structure 6623 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0284300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3402 bp

ATGGCTTCAGCAGGAGGCGGAGGCGGCGGCGGCTGGGGGAAGGCTCAGTCGTCTTCGGGCACCAGCAATTTAAAGCAGCACAACAGCAGCACTCCGGCGGAGTCGGTGAGCAAAGCGGTGGCGCAGTACACGGTCGACGCTCGGCTGCACGCCGTGTTCGAGCAGTCCGAGTCCGGGAAGTCTTTCGACTACTCGCAGAGCATGAGAAGCACCAAAGAATCGGTGCCGGAGCAGCAAATCACGGCGTACCTGTCCAAAATCCAGAGGGGCGGCCATATCCAGCCGTTCGGGTGCACGATTGCCGTGGACGAGTCGACCTTCGGGGTCATTGCCTACAGCGAGAACGCCCGCGACCTGCTCGACCTAATGCCCCAGTCGGTTCCGGTGCTCGAGAGGCGGGAGATCCTCACAATCGGGACCGACGTCAGGACGCTCTTCACGCCTTCCAGCTCGACTTTGCTGGAGAAGGCGTTTGGCGCTCGGGAGATTACTCTGCTCAACCCAATTTGGATACATTCGAGGGTTTCCGGCAAGCCGTTTTACGCGATTTTGCATAGGATTGATGTCGGGGTTGTCATTGATTTGGAGCCTGCTAGAACAGAGGACCCGGCTCTGTCTATAGCCGGGGCCGTGCAGTCTCAGAAGCTGGCCGTGAGGGCGATTTCGCTGCTGCAGTCACTGCCCGGTGGGGATATTAAGCTTTTGTGCGACACGGTGGTGGAGAGTGTGAGGGAGCTTACTGGGTATGATAGAGTTATGGTGTACAAGTTTCATGAGGATGAGCACGGGGAGGTTTTGGCGGAGAGTAAGAGGCCCGATTTGGAGCCTTATCTTGGGCTGCATTATCCGGCCACGGACATACCTCAGGCGTCGAGGTTTTTGTTCAAGCAAAATAGGGTGAGAATGATTGTGGATTGTCATGCCACGCCGGTTCATGTGATCCAGGATGAAGGGCTTATGCAGCCTCTGTGCTTGGTTGGATCAACGCTGCGCGCGCCGCATGGTTGTCATTCCCAGTATATGGCTAATATGGGCTCCATTGCGTCCTTGGCTTTGGCGGTTATCATTAATGGAAATGATGATGAGGCTATTGGTGGACGGAGCTCGATGAGGCTGTGGGGCTTGGTTGTTTGCCATCACACTTCTGCGAGGTGCATCCCGTTTCCTCTTCGGTATGCCTGTGAGTTTCTAATGCAGGCCTTTGGACTACAGTTGAATATGGAATTGCAGTTGGCTTCACAGATGTCGGAGAAACATGTTTTGAGGACTCAGACTCTGTTGTGTGATATGCTTCTTCGTGACACCCCGACTGGGATTATTACTCAAAGTCCTAGTATCATGAATCTTGTCAAGTGTGATGGGGCAGCGCTTTACTACCAAGACAAGTACTACCCAATTGGTGTCACCCCCACTGAAGCTCAGATCAGGGACATTGTTGAGTGGTTGTTGGCTTCACATGGAACTTCGACAGGTTTAAGCACTGACAGTTTGGCTGATGCTGGGTACCCTGGAGCTGCCTCACTTGGTGATGCTGTCTGTGGGATGGCTGCTGCTTATATTACTAAAAGGGACTTTCTCTTCTGGTTCCGATCCCACACTGCAAAAGAGGTGAAATGGGGTGGAGCAAAGCATCATCCTGAGGACAAGGATGATGGGCAGAGGATGCATCCACGTTCTTCCTTTAAGGCGTTTTTGGAAGTGGTTAAAAGCCGTAGTTTGCCATGGGACAATGCAGAAATGGATGCCATACATTCCTTGCAAATTATTTTGCGAGACTCATTTAAGGATGCTGAGACAAACAACTTAAAGGCTGTTACACAGGCCCAGCGTGGGGATCTCGAATATCAAGGGATTGACGAGCTCAGCTCAGTGGCAAGAGAAATGGTTAGATTGATAGAGACTGCAACTGCTCCCATATTTGCTGTAGATGTTAATGGGTGTATAAATGGGTGGAATGCAAAGGTTGCAGAGCTGACAGGGCTCTCAGTTGAGGAGGCTACAGGGAAATCCTTGGTACATGATCTGGTTTACAAAGAATCTGAAGAAACTGTTGACAAACTTCTCCTCCACGCTCTGACAGGAGAAGAAGATAAAAATGTAGAGATAAAACTGAGGACATTTGGCCCAGAGCATGAAAACAAGCCTGTTTTTGTGGTGGTTAATGCTTGCTCTAGCAAGGATTACACTAATAATATCGTGGGAGTTTGCTTTGTTGGTCAGGATGTTACTGGTCAAAAAGTGGTAATGGACAAATTCATAAACATACAAGGTGATTACAAGGCCATTGTTCATAGTCCCAATGCTTTGATCCCGCCCATATTTGCGTCAGATGATAACACATGTTGCTCAGAGTGGAACACTGCCATGGAAAAACTTACTGGGTGGAGCCGTGGAGATATCATTGGAAAAATGTTGGTTGGGGAGGTCTTTGGCAGTTGTTGTCGACTCAAGGGTCCAGATGCTATAACAAAATTCATGATTGTCTTGCACAACGCCATTGGAGGCTTAGACACGGACAAGTTTCCTTTTTCATTCTTTGACCAGAATGGGAAATATGTACAAGCTCTCTTGACAGCAAATAAGAGGGTGAATATAGACGGCCAGGTTATTGGAGCTTTCTGCTTTCTGCAGATTCCTAGTCCAGAACTGCAGCAAGCTCTTAGAGTTCAGAGACAACAGGAGAAGCAATGTTTCACTAGGATGAAAGAATTCGCTTACATATGTCAGGAAATAAAAAATCCTTTAAGTGGTATACGCTTTACTAACTCACTTTTGGAGGCTACAGAGTTGACTGAAGATCAAAAGCAGTTCTTGGAGACTAGTGGTGCTTGTGAGAAGCAAATTTTGAAGATTATAAAGGACGTTGATCTGGCTAGCATTGAAGATGGTTCTTTGGAGCTTGAGAAGGAAGAATTCTTACTTGGAAGTGTCATAAATGCTGTTGTTAGCCAAGTAATGCTATTGCTGAGGGAAAGAAATTTGCAATTGATTCGGGATATTCCTGAAGAAGTCAAAACACTAGCTGTTTATGGTGATCAAGTGAGAATTCAGCAGGTCTTAGCTGATTTCTTATTGAATATGGTACGTTATGCACCATCTGAGGAAGGCTGGGTGGAGATTCATGTTCGTCCAATCTTAACTACAATTCCTGATGGACACTCTATGGTTCATACAGAATTCAGGTTGGTATGCCCTGGTGAAGGTCTTCCCCCTCAATTAGTTCAAGACATGTTCCATAGTTGTCGATGGATGACTCAGGAGGGCCTAGGACTGAGCATGTGCAGGAAGATTCTAAAGCTCATGAAGGGAGAAGTCCAATATATCAGAGAATCAGAGAGATGTTATTTCTTAGTTATTCTCGAGCTTGCTATGCCTCGGATACATGCAAAGAGTGTTGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005911 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010383 GO:0010410 GO:0010411 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0030312 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045493 GO:0046556 GO:0046686 GO:0048046 GO:0050896 GO:0055044 GO:0071554 GO:0071704 GO:0071944 GO:0080176 GO:0097599 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

1133

Amino Acids

125.55

Weight (kDa)

5.59

Isoelectric Point (pI)

45.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAS_2	PF08446	82 - 198	2.7e-37	PAS fold
GAF	PF01590	232 - 409	4.1e-16	GAF domain
PHY	PF00360	422 - 596	1.1e-57	Phytochrome region
PAS	PF00989	627 - 742	1.6e-24	PAS fold
PAS	PF00989	758 - 877	4.6e-20	PAS fold
HisKA	PF00512	902 - 963	1.9e-13	His Kinase A (phospho-acceptor) domain
HATPase_c	PF02518	1011 - 1117	4.4e-13	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008703)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25882
fragaria_vesca	FvH4_4g27501 FvH4_4g27520
malus_domestica	MD13G1042800.v1.1
prunus_persica	Prupe.1G309800_v2.0.a1
pyrus_communis	pycom13g03750 pycom13g03760 pycom16g03780 pycom16g03790
rosa_chinensis	RchiOBHm_Chr4g0435331 RchiOBHm_Chr4g0435341
rosa_laevigata	RLG00000006598
rosa_roxburghii	Rroxscaffold_5G00376600 Rroxscaffold_5G00376610
rosa_rugosa	Rorug04G0284300 Rorug04G0284400
rosa_samantha	Rh4AG339900 Rh4AG340000 Rh4BG348400 Rh4CG363000 Rh4DG342200 Rh4DG342400
rosa_wichuraiana	Rw4G029650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2855
AasI	GACNNNNNNGTC	1 cut(s)	2451
AatII	GACGTC	1 cut(s)	426
Acc36I	ACCTGC	2 cut(s)	363, 3043
Acc65I	GGTACC	1 cut(s)	1503
AccB1I	GGYRCC	3 cut(s)	59, 223, 1503
AccB7I	CCANNNNNTGG	1 cut(s)	1021
AccI	GTMKAC	4 cut(s)	135, 314, 2443, 2752
AccII	CGCG	4 cut(s)	351, 545, 993, 995
AclWI	GGATC	6 cut(s)	397, 934, 988, 1584, 1842, 2302
AcoI	YGGCCR	4 cut(s)	271, 648, 849, 2599
AcsI	RAATTY	8 cut(s)	2252, 2471, 2708, 2841, 2912, 2977, 3045, 3176
AcuI	CTGAAG	4 cut(s)	1434, 2061, 2814, 3024
AcyI	GRCGYC	2 cut(s)	423, 869
AdeI	CACNNNGTG	2 cut(s)	154, 1526
AfaI	GTAC	8 cut(s)	128, 248, 764, 1388, 1505, 2016, 2559, 3084
AfeI	AGCGCT	1 cut(s)	1368
AfiI	CCNNNNNNNGG	9 cut(s)	174, 426, 833, 1021, 1311, 1830, 1831, 2747, 3200
AflIII	ACRYGT	3 cut(s)	1255, 2339, 3228
AgsI	TTSAA	6 cut(s)	886, 1216, 2848, 2882, 3076, 3224
AhlI	ACTAGT	1 cut(s)	2819
AjnI	CCWGG	4 cut(s)	942, 1507, 2601, 3193
AjuI	GAANNNNNNNTTGG	2 cut(s)	2904, 2936
AleI	CACNNNNGTG	1 cut(s)	716
Alw21I	GWGCWC	5 cut(s)	296, 390, 786, 1106, 1863
Alw26I	GTCTC	6 cut(s)	642, 1767, 1787, 1892, 2665, 2810
Alw44I	GTGCAC	1 cut(s)	292
AlwI	GGATC	6 cut(s)	397, 934, 988, 1584, 1842, 2302
AlwNI	CAGNNNCTG	4 cut(s)	646, 679, 967, 1276
Ama87I	CYCGRG	3 cut(s)	389, 480, 3359
Aor51HI	AGCGCT	1 cut(s)	1368
ApaLI	GTGCAC	1 cut(s)	292
ApoI	RAATTY	8 cut(s)	2252, 2471, 2708, 2841, 2912, 2977, 3045, 3176
AseI	ATTAAT	1 cut(s)	1068
Asp700I	GAANNNNTTC	3 cut(s)	182, 2708, 3204
Asp718I	GGTACC	1 cut(s)	1503
AspA2I	CCTAGG	1 cut(s)	3265
AspLEI	GCGC	6 cut(s)	124, 479, 993, 995, 997, 1369
AspS9I	GGNCC	8 cut(s)	417, 604, 627, 813, 1822, 2123, 2453, 3262
AsuC2I	CCSGG	4 cut(s)	175, 608, 625, 684
AsuHPI	GGTGA	9 cut(s)	118, 910, 1396, 1538, 1621, 2282, 2599, 3044, 3209
AsuNHI	GCTAGC	1 cut(s)	2873
AvaI	CYCGRG	3 cut(s)	389, 480, 3359
AvaII	GGWCC	3 cut(s)	417, 604, 2453
AvrII	CCTAGG	1 cut(s)	3265
AxyI	CCTNAGG	2 cut(s)	864, 1638
BaeGI	GKGCMC	2 cut(s)	62, 296
BanI	GGYRCC	3 cut(s)	59, 223, 1503
BanII	GRGCYC	4 cut(s)	1037, 1106, 1863, 1983
BarI	GAAGNNNNNNTAC	2 cut(s)	2901, 2933
BbsI	GAAGAC	2 cut(s)	45, 3197
Bbv12I	GWGCWC	5 cut(s)	296, 390, 786, 1106, 1863
BbvCI	CCTCAGC	1 cut(s)	2966
BccI	CCATC	8 cut(s)	1143, 1352, 1537, 1646, 2879, 3103, 3146, 3240
BcgI	CGANNNNNNTGC	4 cut(s)	649, 683, 2972, 3006
BciT130I	CCWGG	4 cut(s)	944, 1509, 2603, 3195
BciVI	GTATCC	2 cut(s)	504, 3372
BclI	TGATCA	1 cut(s)	3034
BcnI	CCSGG	4 cut(s)	175, 608, 625, 684
BcoDI	GTCTC	6 cut(s)	642, 1767, 1787, 1892, 2665, 2810
BcuI	ACTAGT	1 cut(s)	2819
BfmI	CTRYAG	9 cut(s)	334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
BfoI	RGCGCY	2 cut(s)	480, 1370
BfuAI	ACCTGC	2 cut(s)	363, 3043
BfuI	GTATCC	2 cut(s)	504, 3372
BglI	GCCNNNNNGGC	1 cut(s)	656
BlnI	CCTAGG	1 cut(s)	3265
BlpI	GCTNAGC	1 cut(s)	1862
BmcAI	AGTACT	1 cut(s)	1388
Bme1390I	CCNGG	8 cut(s)	175, 608, 625, 684, 944, 1509, 2603, 3195
Bme18I	GGWCC	3 cut(s)	417, 604, 2453
BmeT110I	CYCGRG	3 cut(s)	389, 480, 3359
BmgT120I	GGNCC	8 cut(s)	417, 604, 627, 813, 1822, 2123, 2453, 3262
BmrFI	CCNGG	8 cut(s)	175, 608, 625, 684, 944, 1509, 2603, 3195
BmrI	ACTGGG	5 cut(s)	367, 750, 1009, 1320, 2388
BmsI	GCATC	9 cut(s)	1164, 1486, 1519, 1639, 1653, 1675, 1732, 1777, 2449
BmtI	GCTAGC	1 cut(s)	2877
BmuI	ACTGGG	5 cut(s)	367, 750, 1009, 1320, 2388
BoxI	GACNNNNGTC	1 cut(s)	422
BpiI	GAAGAC	2 cut(s)	45, 3197
BplI	GAGNNNNNCTC	2 cut(s)	1850, 1882
BpmI	CTGGAG	2 cut(s)	482, 1530
Bpu10I	CCTNAGC	1 cut(s)	2966
Bpu1102I	GCTNAGC	1 cut(s)	1862
BpuEI	CTTGAG	2 cut(s)	2432, 2921
BpuMI	CCSGG	4 cut(s)	175, 608, 625, 684
BsaHI	GRCGYC	2 cut(s)	423, 869
BsaWI	WCCGGW	1 cut(s)	382
BsaXI	ACNNNNNCTCC	4 cut(s)	455, 485, 2049, 2079
Bsc4I	CCNNNNNNNGG	9 cut(s)	174, 426, 833, 1021, 1311, 1830, 1831, 2747, 3200
Bse118I	RCCGGY	1 cut(s)	928
Bse1I	ACTGG	6 cut(s)	373, 745, 1015, 1315, 2233, 2383
Bse21I	CCTNAGG	2 cut(s)	864, 1638
Bse3DI	GCAATG	3 cut(s)	327, 1038, 2693
BseBI	CCWGG	4 cut(s)	944, 1509, 2603, 3195
BseLI	CCNNNNNNNGG	9 cut(s)	174, 426, 833, 1021, 1311, 1830, 1831, 2747, 3200
BseMI	GCAATG	3 cut(s)	327, 1038, 2693
BseNI	ACTGG	6 cut(s)	373, 745, 1015, 1315, 2233, 2383
BsePI	GCGCGC	2 cut(s)	991, 993
BseRI	GAGGAG	2 cut(s)	2006, 2054
BseSI	GKGCMC	2 cut(s)	62, 296
BseYI	CCCAGC	4 cut(s)	33, 1499, 1824, 3108
BsgI	GTGCAG	3 cut(s)	131, 653, 3301
Bsh1236I	CGCG	4 cut(s)	351, 545, 993, 995
Bsh1285I	CGRYCG	1 cut(s)	135
BshNI	GGYRCC	3 cut(s)	59, 223, 1503
BsiEI	CGRYCG	1 cut(s)	135
BsiHKAI	GWGCWC	5 cut(s)	296, 390, 786, 1106, 1863
BsiHKCI	CYCGRG	3 cut(s)	389, 480, 3359
BslFI	GGGAC	5 cut(s)	430, 1442, 1583, 1739, 2280
BslI	CCNNNNNNNGG	9 cut(s)	174, 426, 833, 1021, 1311, 1830, 1831, 2747, 3200
BsmAI	GTCTC	6 cut(s)	642, 1767, 1787, 1892, 2665, 2810
BsmFI	GGGAC	5 cut(s)	430, 1442, 1583, 1739, 2280
BsmI	GAATGC	1 cut(s)	1960
BsoBI	CYCGRG	3 cut(s)	389, 480, 3359
Bsp1286I	GDGCHC	8 cut(s)	62, 296, 390, 786, 1037, 1106, 1863, 1983
Bsp1407I	TGTACA	2 cut(s)	762, 2557
Bsp1720I	GCTNAGC	1 cut(s)	1862
Bsp19I	CCATGG	2 cut(s)	1721, 2364
BspFNI	CGCG	4 cut(s)	351, 545, 993, 995
BspHI	TCATGA	4 cut(s)	772, 1338, 2475, 3300
BspMAI	CTGCAG	3 cut(s)	675, 2631, 2652
BspMI	ACCTGC	2 cut(s)	363, 3043
BspOI	GCTAGC	1 cut(s)	2877
BspPI	GGATC	6 cut(s)	397, 934, 988, 1584, 1842, 2302
BspQI	GCTCTTC	1 cut(s)	440
BspT107I	GGYRCC	3 cut(s)	59, 223, 1503
BsrDI	GCAATG	3 cut(s)	327, 1038, 2693
BsrFI	RCCGGY	1 cut(s)	928
BsrGI	TGTACA	2 cut(s)	762, 2557
BsrI	ACTGG	6 cut(s)	373, 745, 1015, 1315, 2233, 2383
BssAI	RCCGGY	1 cut(s)	928
BssHII	GCGCGC	2 cut(s)	991, 993
BssNAI	GTATAC	1 cut(s)	2753
BssNI	GRCGYC	2 cut(s)	423, 869
BssT1I	CCWWGG	5 cut(s)	1047, 1721, 2010, 2364, 3265
Bst1107I	GTATAC	1 cut(s)	2753
Bst2UI	CCWGG	4 cut(s)	944, 1509, 2603, 3195
Bst4CI	ACNGT	5 cut(s)	133, 715, 1212, 1487, 2050
Bst6I	CTCTTC	3 cut(s)	440, 1173, 1583
BstACI	GRCGYC	2 cut(s)	423, 869
BstAPI	GCANNNNNTGC	3 cut(s)	679, 1663, 1964
BstAUI	TGTACA	2 cut(s)	762, 2557
BstDSI	CCRYGG	5 cut(s)	303, 852, 1721, 2364, 2389
BstENI	CCTNNNNNAGG	1 cut(s)	3198
BstFNI	CGCG	4 cut(s)	351, 545, 993, 995
BstH2I	RGCGCY	2 cut(s)	480, 1370
BstHHI	GCGC	6 cut(s)	124, 479, 993, 995, 997, 1369
BstMAI	GTCTC	6 cut(s)	642, 1767, 1787, 1892, 2665, 2810
BstMCI	CGRYCG	1 cut(s)	135
BstNI	CCWGG	4 cut(s)	944, 1509, 2603, 3195
BstNSI	RCATGY	5 cut(s)	1259, 2343, 3232, 3280, 3386
BstPAI	GACNNNNGTC	1 cut(s)	422
BstSCI	CCNGG	8 cut(s)	173, 606, 623, 682, 942, 1507, 2601, 3193
BstSFI	CTRYAG	9 cut(s)	334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
BstSLI	GKGCMC	2 cut(s)	62, 296
BstUI	CGCG	4 cut(s)	351, 545, 993, 995
BstV2I	GAAGAC	2 cut(s)	45, 3197
BstX2I	RGATCY	2 cut(s)	402, 1834
BstXI	CCANNNNNNTGG	1 cut(s)	2609
BstYI	RGATCY	2 cut(s)	402, 1834
BstZ17I	GTATAC	1 cut(s)	2753
Bsu36I	CCTNAGG	2 cut(s)	864, 1638
BsuI	GTATCC	2 cut(s)	504, 3372
BtgI	CCRYGG	5 cut(s)	303, 852, 1721, 2364, 2389
BtsI	GCAGTG	3 cut(s)	677, 1596, 2358
BtsIMutI	CAGTG	6 cut(s)	677, 1410, 1479, 1596, 1875, 2358
BveI	ACCTGC	2 cut(s)	363, 3043
CaiI	CAGNNNCTG	4 cut(s)	646, 679, 967, 1276
CciI	TCATGA	4 cut(s)	772, 1338, 2475, 3300
CfoI	GCGC	6 cut(s)	124, 479, 993, 995, 997, 1369
Cfr10I	RCCGGY	1 cut(s)	928
Cfr13I	GGNCC	8 cut(s)	417, 604, 627, 813, 1822, 2123, 2453, 3262
CseI	GACGC	5 cut(s)	146, 439, 858, 1032, 2313
Csp6I	GTAC	8 cut(s)	127, 247, 763, 1387, 1504, 2015, 2558, 3083
CviQI	GTAC	8 cut(s)	127, 247, 763, 1387, 1504, 2015, 2558, 3083
DraI	TTTAAA	1 cut(s)	72
DraIII	CACNNNGTG	2 cut(s)	154, 1526
DrdI	GACNNNNNNGTC	1 cut(s)	2451
DseDI	GACNNNNNNGTC	1 cut(s)	2451
EaeI	YGGCCR	4 cut(s)	271, 648, 849, 2599
Eam1104I	CTCTTC	3 cut(s)	440, 1173, 1583
EarI	CTCTTC	3 cut(s)	440, 1173, 1583
EciI	GGCGGA	3 cut(s)	33, 113, 815
Ecl136II	GAGCTC	2 cut(s)	1104, 1861
Eco130I	CCWWGG	5 cut(s)	1047, 1721, 2010, 2364, 3265
Eco147I	AGGCCT	1 cut(s)	1199
Eco24I	GRGCYC	4 cut(s)	1037, 1106, 1863, 1983
Eco32I	GATATC	1 cut(s)	2398
Eco47I	GGWCC	3 cut(s)	417, 604, 2453
Eco47III	AGCGCT	1 cut(s)	1368
Eco53kI	GAGCTC	2 cut(s)	1104, 1861
Eco57I	CTGAAG	4 cut(s)	1434, 2061, 2814, 3024
Eco81I	CCTNAGG	2 cut(s)	864, 1638
Eco88I	CYCGRG	3 cut(s)	389, 480, 3359
EcoICRI	GAGCTC	2 cut(s)	1104, 1861
EcoNI	CCTNNNNNAGG	1 cut(s)	3198
EcoO109I	RGGNCCY	2 cut(s)	604, 3262
EcoRI	GAATTC	4 cut(s)	2708, 2912, 3045, 3176
EcoRII	CCWGG	4 cut(s)	942, 1507, 2601, 3193
EcoRV	GATATC	1 cut(s)	2398
EcoT14I	CCWWGG	5 cut(s)	1047, 1721, 2010, 2364, 3265
EcoT22I	ATGCAT	1 cut(s)	1668
EcoT38I	GRGCYC	4 cut(s)	1037, 1106, 1863, 1983
ErhI	CCWWGG	5 cut(s)	1047, 1721, 2010, 2364, 3265
FalI	AAGNNNNNCTT	2 cut(s)	2795, 2827
FaqI	GGGAC	5 cut(s)	430, 1442, 1583, 1739, 2280
FauI	CCCGC	3 cut(s)	356, 390, 2319
FauNDI	CATATG	1 cut(s)	2720
FbaI	TGATCA	1 cut(s)	3034
FblI	GTMKAC	4 cut(s)	135, 314, 2443, 2752
FriOI	GRGCYC	4 cut(s)	1037, 1106, 1863, 1983
GlaI	GCGC	6 cut(s)	123, 478, 992, 994, 996, 1368
GsaI	CCCAGC	4 cut(s)	37, 1503, 1828, 3112
GsuI	CTGGAG	2 cut(s)	482, 1530
HaeII	RGCGCY	2 cut(s)	480, 1370
HgaI	GACGC	5 cut(s)	146, 439, 858, 1032, 2313
HhaI	GCGC	6 cut(s)	124, 479, 993, 995, 997, 1369
Hin1I	GRCGYC	2 cut(s)	423, 869
Hin6I	GCGC	6 cut(s)	122, 477, 991, 993, 995, 1367
HinP1I	GCGC	6 cut(s)	122, 477, 991, 993, 995, 1367
HincII	GTYRAC	6 cut(s)	136, 315, 2053, 2444, 2790, 3397
HindII	GTYRAC	6 cut(s)	136, 315, 2053, 2444, 2790, 3397
HindIII	AAGCTT	1 cut(s)	698
HphI	GGTGA	9 cut(s)	118, 910, 1396, 1538, 1621, 2282, 2599, 3044, 3209
Hpy99I	CGWCG	3 cut(s)	140, 425, 874
HpyAV	CCTTC	9 cut(s)	34, 328, 453, 460, 944, 2899, 3098, 3194, 3298
HpyCH4III	ACNGT	5 cut(s)	133, 715, 1212, 1487, 2050
HpyCH4IV	ACGT	4 cut(s)	423, 1672, 2862, 3085
HpySE526I	ACGT	4 cut(s)	423, 1672, 2862, 3085
Hsp92I	GRCGYC	2 cut(s)	423, 869
HspAI	GCGC	6 cut(s)	122, 477, 991, 993, 995, 1367
KpnI	GGTACC	1 cut(s)	1507
Ksp22I	TGATCA	1 cut(s)	3034
LguI	GCTCTTC	1 cut(s)	440
LweI	GCATC	9 cut(s)	1164, 1486, 1519, 1639, 1653, 1675, 1732, 1777, 2449
MaeII	ACGT	4 cut(s)	423, 1672, 2862, 3085
MaeIII	GTNAC	5 cut(s)	675, 1298, 1402, 1813, 2224
MfeI	CAATTG	2 cut(s)	1395, 2984
MflI	RGATCY	2 cut(s)	402, 1834
MhlI	GDGCHC	8 cut(s)	62, 296, 390, 786, 1037, 1106, 1863, 1983
MlyI	GAGTC	8 cut(s)	110, 179, 320, 1261, 1267, 1769, 2439, 3247
MmeI	TCCRAC	2 cut(s)	958, 1227
Mph1103I	ATGCAT	1 cut(s)	1668
MroXI	GAANNNNTTC	3 cut(s)	182, 2708, 3204
MslI	CAYNNNNRTG	4 cut(s)	716, 777, 2363, 2699
MspR9I	CCNGG	8 cut(s)	175, 608, 625, 684, 944, 1509, 2603, 3195
MunI	CAATTG	2 cut(s)	1395, 2984
Mva1269I	GAATGC	1 cut(s)	1960
MvaI	CCWGG	4 cut(s)	944, 1509, 2603, 3195
MvnI	CGCG	4 cut(s)	351, 545, 993, 995
NciI	CCSGG	4 cut(s)	175, 608, 625, 684
NcoI	CCATGG	2 cut(s)	1721, 2364
NdeI	CATATG	1 cut(s)	2720
NheI	GCTAGC	1 cut(s)	2873
NmeAIII	GCCGAG	1 cut(s)	121
NmuCI	GTSAC	3 cut(s)	675, 1298, 1402
NsiI	ATGCAT	1 cut(s)	1668
NspI	RCATGY	5 cut(s)	1259, 2343, 3232, 3280, 3386
OliI	CACNNNNGTG	1 cut(s)	716
PaeR7I	CTCGAG	2 cut(s)	389, 3359
PagI	TCATGA	4 cut(s)	772, 1338, 2475, 3300
PauI	GCGCGC	2 cut(s)	991, 993
PceI	AGGCCT	1 cut(s)	1199
PciI	ACATGT	3 cut(s)	1255, 2339, 3228
PciSI	GCTCTTC	1 cut(s)	440
PcsI	WCGNNNNNNNCGW	3 cut(s)	156, 165, 420
PctI	GAATGC	1 cut(s)	1960
PdmI	GAANNNNTTC	3 cut(s)	182, 2708, 3204
PfeI	GAWTC	8 cut(s)	218, 1342, 2036, 2632, 2989, 3118, 3289, 3329
PflMI	CCANNNNNTGG	1 cut(s)	1021
PfoI	TCCNGGA	2 cut(s)	173, 942
PleI	GAGTC	8 cut(s)	109, 178, 319, 1261, 1267, 1769, 2439, 3247
PpsI	GAGTC	8 cut(s)	109, 178, 319, 1261, 1267, 1769, 2439, 3247
PpuMI	RGGWCCY	1 cut(s)	604
PscI	ACATGT	3 cut(s)	1255, 2339, 3228
PshAI	GACNNNNGTC	1 cut(s)	422
PshBI	ATTAAT	1 cut(s)	1068
PsiI	TTATAA	1 cut(s)	2855
Psp124BI	GAGCTC	2 cut(s)	1106, 1863
Psp5II	RGGWCCY	1 cut(s)	604
Psp6I	CCWGG	4 cut(s)	942, 1507, 2601, 3193
PspFI	CCCAGC	4 cut(s)	33, 1499, 1824, 3108
PspGI	CCWGG	4 cut(s)	942, 1507, 2601, 3193
PspPI	GGNCC	8 cut(s)	417, 604, 627, 813, 1822, 2123, 2453, 3262
PspPPI	RGGWCCY	1 cut(s)	604
PstI	CTGCAG	3 cut(s)	675, 2631, 2652
PstNI	CAGNNNCTG	4 cut(s)	646, 679, 967, 1276
PsuI	RGATCY	2 cut(s)	402, 1834
PteI	GCGCGC	2 cut(s)	991, 993
RsaI	GTAC	8 cut(s)	128, 248, 764, 1388, 1505, 2016, 2559, 3084
RsaNI	GTAC	8 cut(s)	127, 247, 763, 1387, 1504, 2015, 2558, 3083
RseI	CAYNNNNRTG	4 cut(s)	716, 777, 2363, 2699
SacI	GAGCTC	2 cut(s)	1106, 1863
SalI	GTCGAC	3 cut(s)	134, 313, 2442
SapI	GCTCTTC	1 cut(s)	440
Sau96I	GGNCC	8 cut(s)	417, 604, 627, 813, 1822, 2123, 2453, 3262
ScaI	AGTACT	1 cut(s)	1388
SchI	GAGTC	8 cut(s)	110, 179, 320, 1261, 1267, 1769, 2439, 3247
ScrFI	CCNGG	8 cut(s)	175, 608, 625, 684, 944, 1509, 2603, 3195
SduI	GDGCHC	8 cut(s)	62, 296, 390, 786, 1037, 1106, 1863, 1983
SfaNI	GCATC	9 cut(s)	1164, 1486, 1519, 1639, 1653, 1675, 1732, 1777, 2449
SfcI	CTRYAG	9 cut(s)	334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
Sfr274I	CTCGAG	2 cut(s)	389, 3359
SinI	GGWCC	3 cut(s)	417, 604, 2453
SlaI	CTCGAG	2 cut(s)	389, 3359
SmiMI	CAYNNNNRTG	4 cut(s)	716, 777, 2363, 2699
SmlI	CTYRAG	4 cut(s)	389, 2447, 2900, 3359
SmoI	CTYRAG	4 cut(s)	389, 2447, 2900, 3359
SpeI	ACTAGT	1 cut(s)	2819
SseBI	AGGCCT	1 cut(s)	1199
SstI	GAGCTC	2 cut(s)	1106, 1863
StuI	AGGCCT	1 cut(s)	1199
StyD4I	CCNGG	8 cut(s)	173, 606, 623, 682, 942, 1507, 2601, 3193
StyI	CCWWGG	5 cut(s)	1047, 1721, 2010, 2364, 3265
TaaI	ACNGT	5 cut(s)	133, 715, 1212, 1487, 2050
TaiI	ACGT	4 cut(s)	426, 1675, 2865, 3088
TaqII	GACCGA	1 cut(s)	434
TatI	WGTACW	4 cut(s)	126, 762, 1386, 2557
TauI	GCSGC	5 cut(s)	27, 30, 33, 273, 1000
TfiI	GAWTC	8 cut(s)	218, 1342, 2036, 2632, 2989, 3118, 3289, 3329
TscAI	CASTG	6 cut(s)	684, 1417, 1486, 1603, 1875, 2365
TseFI	GTSAC	3 cut(s)	675, 1298, 1402
Tsp45I	GTSAC	3 cut(s)	675, 1298, 1402
TspGWI	ACGGA	3 cut(s)	869, 1114, 2528
TspRI	CASTG	6 cut(s)	684, 1417, 1486, 1603, 1875, 2365
Van91I	CCANNNNNTGG	1 cut(s)	1021
VneI	GTGCAC	1 cut(s)	292
VpaK11BI	GGWCC	3 cut(s)	417, 604, 2453
VspI	ATTAAT	1 cut(s)	1068
XagI	CCTNNNNNAGG	1 cut(s)	3198
XapI	RAATTY	8 cut(s)	2252, 2471, 2708, 2841, 2912, 2977, 3045, 3176
XceI	RCATGY	5 cut(s)	1259, 2343, 3232, 3280, 3386
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1045
XhoI	CTCGAG	2 cut(s)	389, 3359
XmaJI	CCTAGG	1 cut(s)	3265
XmiI	GTMKAC	4 cut(s)	135, 314, 2443, 2752
XmnI	GAANNNNTTC	3 cut(s)	182, 2708, 3204
ZraI	GACGTC	1 cut(s)	424
ZrmI	AGTACT	1 cut(s)	1388
Zsp2I	ATGCAT	1 cut(s)	1668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.