Rh4AG339900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008703
Physical Location & Seq
Forward (+)
64927742 .. 64928062
321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG339900.1

Sequence Viewer

Length: 321 bp
ATGGCGGCAGAGAAGAGAAAGCAGGCCGGCAACGACGTCGTCTCGGGGAGGCACAACCATAAGAAGGCGCGGGATGAAACGACAGTGAACGAGGACGAGGTTCAGGAGTTCTTCGCTATCATCAAGCGCATACACGTGGCAATCAACTACTTTAAGAGCGCTAACGGCGGTGGAAGTAAATATGTGGACGGAGAGTTGAGGGCAATGATCGAGAGTAACGAGGAGGCGGACGGCGTCAAAGGGATGGCCAAGACTACGAAGGAGGAGGATGTGGGGTTCGATTTGAACGCGGAACCGGACCCGGAATTTGATCTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

11.74

Weight (kDa)

4.94

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 39
AccII CGCG 2 cut(s) 70, 290
AciI CCGC 5 cut(s) 5, 70, 168, 227, 290
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 305
AcvI CACGTG 1 cut(s) 136
AcyI GRCGYC 2 cut(s) 36, 234
AfeI AGCGCT 1 cut(s) 160
AfiI CCNNNNNNNGG 1 cut(s) 64
AflIII ACRYGT 1 cut(s) 133
AgsI TTSAA 1 cut(s) 286
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
Alw26I GTCTC 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 43
Aor51HI AGCGCT 1 cut(s) 160
AoxI GGCC 2 cut(s) 24, 246
ApoI RAATTY 1 cut(s) 305
ArsI GACNNNNNNTTYG 1 cut(s) 290
AspLEI GCGC 3 cut(s) 70, 129, 161
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 1 cut(s) 298
BalI TGGCCA 1 cut(s) 248
BbrPI CACGTG 1 cut(s) 136
BccI CCATC 1 cut(s) 238
BceAI ACGGC 2 cut(s) 181, 247
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 46
BfoI RGCGCY 1 cut(s) 162
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 2 cut(s) 294, 300
BmrFI CCNGG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 302
BsaAI YACGTR 1 cut(s) 136
BsaHI GRCGYC 2 cut(s) 36, 234
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse118I RCCGGY 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 210
BseGI GGATG 3 cut(s) 79, 249, 274
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 305
BseRI GAGGAG 2 cut(s) 236, 278
Bsh1236I CGCG 2 cut(s) 70, 290
BshFI GGCC 2 cut(s) 26, 248
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 3 cut(s) 27, 296, 302
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 46
BsmBI CGTCTC 1 cut(s) 46
BsnI GGCC 2 cut(s) 26, 248
BsoBI CYCGRG 1 cut(s) 43
Bsp143I GATC 2 cut(s) 207, 310
BspACI CCGC 5 cut(s) 5, 70, 168, 227, 290
BspANI GGCC 2 cut(s) 26, 248
BspCNI CTCAG 1 cut(s) 306
BspFNI CGCG 2 cut(s) 70, 290
BspLI GGNNCC 2 cut(s) 294, 300
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 26
BssMI GATC 2 cut(s) 207, 310
BssNI GRCGYC 2 cut(s) 36, 234
Bst4CI ACNGT 1 cut(s) 85
Bst6I CTCTTC 1 cut(s) 8
BstACI GRCGYC 2 cut(s) 36, 234
BstBAI YACGTR 1 cut(s) 136
BstC8I GCNNGC 2 cut(s) 24, 28
BstDEI CTNAG 1 cut(s) 314
BstF5I GGATG 3 cut(s) 79, 249, 274
BstFNI CGCG 2 cut(s) 70, 290
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 3 cut(s) 70, 129, 161
BstKTI GATC 2 cut(s) 210, 313
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 2 cut(s) 207, 310
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 300
BstUI CGCG 2 cut(s) 70, 290
BsuRI GGCC 2 cut(s) 26, 248
BtsCI GGATG 3 cut(s) 79, 249, 274
BtsIMutI CAGTG 1 cut(s) 90
Cac8I GCNNGC 2 cut(s) 24, 28
CfoI GCGC 3 cut(s) 70, 129, 161
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 1 cut(s) 223
CviJI RGCY 2 cut(s) 26, 248
CviKI_1 RGCY 2 cut(s) 26, 248
DdeI CTNAG 1 cut(s) 314
DpnI GATC 2 cut(s) 209, 312
DpnII GATC 2 cut(s) 207, 310
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
EciI GGCGGA 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 298
Eco47III AGCGCT 1 cut(s) 160
Eco72I CACGTG 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 43
Esp3I CGTCTC 1 cut(s) 46
FaiI YATR 3 cut(s) 60, 131, 183
FauI CCCGC 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 3 cut(s) 86, 256, 281
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 3 cut(s) 69, 128, 160
GluI GCNGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 2 cut(s) 26, 248
HapII CCGG 3 cut(s) 27, 296, 302
HgaI GACGC 1 cut(s) 223
HhaI GCGC 3 cut(s) 70, 129, 161
Hin1I GRCGYC 2 cut(s) 36, 234
Hin6I GCGC 3 cut(s) 68, 127, 159
HinP1I GCGC 3 cut(s) 68, 127, 159
HpaII CCGG 3 cut(s) 27, 296, 302
Hpy166II GTNNAC 2 cut(s) 88, 187
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 2 cut(s) 104, 211
Hpy8I GTNNAC 2 cut(s) 88, 187
Hpy99I CGWCG 2 cut(s) 38, 41
HpyAV CCTTC 2 cut(s) 58, 253
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4IV ACGT 2 cut(s) 36, 135
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 2 cut(s) 36, 135
Hsp92I GRCGYC 2 cut(s) 36, 234
HspAI GCGC 3 cut(s) 68, 127, 159
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Kzo9I GATC 2 cut(s) 207, 310
LpnPI CCDG 5 cut(s) 8, 40, 89, 309, 315
MaeII ACGT 2 cut(s) 36, 135
MaeIII GTNAC 1 cut(s) 215
MalI GATC 2 cut(s) 209, 312
MboI GATC 2 cut(s) 207, 310
MboII GAAGA 2 cut(s) 25, 103
MlsI TGGCCA 1 cut(s) 248
MluCI AATT 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 248
MnlI CCTC 8 cut(s) 42, 85, 91, 192, 214, 217, 256, 259
Mox20I TGGCCA 1 cut(s) 248
MroNI GCCGGC 1 cut(s) 26
MscI TGGCCA 1 cut(s) 248
MseI TTAA 2 cut(s) 153, 319
MslI CAYNNNNRTG 1 cut(s) 134
Msp20I TGGCCA 1 cut(s) 248
MspI CCGG 3 cut(s) 27, 296, 302
MspR9I CCNGG 1 cut(s) 302
MvnI CGCG 2 cut(s) 70, 290
MwoI GCNNNNNNNGC 1 cut(s) 165
NaeI GCCGGC 1 cut(s) 28
NciI CCSGG 1 cut(s) 302
NdeII GATC 2 cut(s) 207, 310
NgoMIV GCCGGC 1 cut(s) 26
NlaIV GGNNCC 2 cut(s) 294, 300
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdiI GCCGGC 1 cut(s) 28
PflFI GACNNNGTC 2 cut(s) 38, 233
PkrI GCNGC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 136
PmlI CACGTG 1 cut(s) 136
Ppu21I YACGTR 1 cut(s) 136
PspCI CACGTG 1 cut(s) 136
PspN4I GGNNCC 2 cut(s) 294, 300
PspPI GGNCC 1 cut(s) 298
PsyI GACNNNGTC 2 cut(s) 38, 233
RseI CAYNNNNRTG 1 cut(s) 134
SaqAI TTAA 2 cut(s) 153, 319
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 2 cut(s) 207, 310
Sau96I GGNCC 1 cut(s) 298
ScrFI CCNGG 1 cut(s) 302
SetI ASST 3 cut(s) 39, 102, 138
SinI GGWCC 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 134
Sse9I AATT 1 cut(s) 305
SsiI CCGC 5 cut(s) 5, 70, 168, 227, 290
StyD4I CCNGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 85
TaiI ACGT 2 cut(s) 39, 138
TaqI TCGA 2 cut(s) 210, 279
TasI AATT 1 cut(s) 305
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 153, 319
Tru9I TTAA 2 cut(s) 153, 319
TscAI CASTG 1 cut(s) 90
TspDTI ATGAA 1 cut(s) 90
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 1 cut(s) 90
Tth111I GACNNNGTC 2 cut(s) 38, 233
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 1 cut(s) 305
ZraI GACGTC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.