RchiOBHm_Chr4g0435341

NIM1-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008703
Physical Location & Seq
Forward (+)
58939439 .. 58939931
493 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40377

Sequence Viewer

Length: 306 bp
ATGGATGCAGAAAAGCGAAAGCGCGACGATGAGGGCGAAATTGACGGGAAGAGAGCGAAATCGGAGGACGATAACGGCGCGGTGGTGACGGAGGAGGAGGTGGAGGAGTTCTTTACCATAATCAGGAGGATACACGTGGCGGCCAGGCAGTTTGGGAAACCGGAAGGAGCAGCCGGCCCTAAGTTGACGACGACATCAGAGAAATTGAGGCTCTCTTTCGAAGCAAGAGACTTTGAGGAAGAGAAAAGGCCGGAGGCGGATTCGGGTTTGGATCTCAATTCGGATCCTCCATCGGAAGAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.34

Weight (kDa)

4.58

Isoelectric Point (pI)

66.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 24, 80
AciI CCGC 3 cut(s) 80, 140, 257
AclWI GGATC 3 cut(s) 278, 279, 291
AcoI YGGCCR 1 cut(s) 141
AcvI CACGTG 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 123
AflIII ACRYGT 1 cut(s) 133
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 1 cut(s) 303
AluI AGCT 1 cut(s) 303
Alw26I GTCTC 1 cut(s) 222
AlwI GGATC 3 cut(s) 278, 279, 291
AoxI GGCC 3 cut(s) 141, 175, 248
ApeKI GCWGC 1 cut(s) 170
AspLEI GCGC 2 cut(s) 24, 80
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 97
AsuII TTCGAA 1 cut(s) 219
BamHI GGATCC 1 cut(s) 283
BbrPI CACGTG 1 cut(s) 136
BbvI GCAGC 1 cut(s) 182
BccI CCATC 1 cut(s) 298
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 145
BciVI GTATCC 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 222
BfuI GTATCC 1 cut(s) 123
BisI GCNGC 2 cut(s) 141, 171
BlsI GCNGC 2 cut(s) 142, 172
Bme1390I CCNGG 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 136
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse118I RCCGGY 1 cut(s) 173
BseBI CCWGG 1 cut(s) 145
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 123
BseRI GAGGAG 3 cut(s) 107, 110, 119
BseXI GCAGC 1 cut(s) 182
Bsh1236I CGCG 2 cut(s) 24, 80
BshFI GGCC 3 cut(s) 143, 177, 250
BsiSI CCGG 3 cut(s) 161, 174, 251
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 222
BsnI GGCC 3 cut(s) 143, 177, 250
Bsp119I TTCGAA 1 cut(s) 219
Bsp143I GATC 2 cut(s) 271, 283
BspACI CCGC 3 cut(s) 80, 140, 257
BspANI GGCC 3 cut(s) 143, 177, 250
BspFNI CGCG 2 cut(s) 24, 80
BspLI GGNNCC 1 cut(s) 285
BspPI GGATC 3 cut(s) 278, 279, 291
BspT104I TTCGAA 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssMI GATC 2 cut(s) 271, 283
Bst2UI CCWGG 1 cut(s) 145
Bst6I CTCTTC 2 cut(s) 44, 234
BstBAI YACGTR 1 cut(s) 136
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 175
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 24, 80
BstHHI GCGC 2 cut(s) 24, 80
BstKTI GATC 2 cut(s) 274, 286
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 2 cut(s) 271, 283
BstNI CCWGG 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 143
BstUI CGCG 2 cut(s) 24, 80
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 271, 283
BstYI RGATCY 2 cut(s) 271, 283
BsuI GTATCC 1 cut(s) 123
BsuRI GGCC 3 cut(s) 143, 177, 250
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 1 cut(s) 175
CfoI GCGC 2 cut(s) 24, 80
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 176
CviJI RGCY 6 cut(s) 143, 173, 177, 211, 250, 303
CviKI_1 RGCY 6 cut(s) 143, 173, 177, 211, 250, 303
DdeI CTNAG 1 cut(s) 180
DpnI GATC 2 cut(s) 273, 285
DpnII GATC 2 cut(s) 271, 283
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 2 cut(s) 44, 234
EarI CTCTTC 2 cut(s) 44, 234
EciI GGCGGA 1 cut(s) 272
Eco72I CACGTG 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 143
FaiI YATR 1 cut(s) 119
Fnu4HI GCNGC 2 cut(s) 141, 171
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 2 cut(s) 141, 171
GlaI GCGC 2 cut(s) 23, 79
GluI GCNGC 2 cut(s) 141, 171
HaeIII GGCC 3 cut(s) 143, 177, 250
HapII CCGG 3 cut(s) 161, 174, 251
HhaI GCGC 2 cut(s) 24, 80
Hin6I GCGC 2 cut(s) 22, 78
HinP1I GCGC 2 cut(s) 22, 78
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HinfI GANTC 1 cut(s) 260
HpaII CCGG 3 cut(s) 161, 174, 251
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 4 cut(s) 64, 199, 283, 295
Hpy188III TCNNGA 1 cut(s) 124
Hpy8I GTNNAC 1 cut(s) 186
Hpy99I CGWCG 2 cut(s) 29, 193
HpyAV CCTTC 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 1 cut(s) 8
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 1 cut(s) 135
HspAI GCGC 2 cut(s) 22, 78
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 271, 283
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 6 cut(s) 109, 130, 157, 174, 187, 264
Lsp1109I GCAGC 1 cut(s) 182
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 85
MalI GATC 2 cut(s) 273, 285
MboI GATC 2 cut(s) 271, 283
MboII GAAGA 2 cut(s) 61, 251
MflI RGATCY 2 cut(s) 271, 283
MluCI AATT 3 cut(s) 39, 203, 277
MroNI GCCGGC 1 cut(s) 173
MspI CCGG 3 cut(s) 161, 174, 251
MspR9I CCNGG 1 cut(s) 145
MvaI CCWGG 1 cut(s) 145
MvnI CGCG 2 cut(s) 24, 80
NaeI GCCGGC 1 cut(s) 175
NdeII GATC 2 cut(s) 271, 283
NgoMIV GCCGGC 1 cut(s) 173
NlaIV GGNNCC 1 cut(s) 285
NmuCI GTSAC 1 cut(s) 85
NspV TTCGAA 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 2 cut(s) 142, 172
PmaCI CACGTG 1 cut(s) 136
PmlI CACGTG 1 cut(s) 136
Ppu21I YACGTR 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 143
PspCI CACGTG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 1 cut(s) 176
PsuI RGATCY 2 cut(s) 271, 283
SatI GCNGC 2 cut(s) 141, 171
Sau3AI GATC 2 cut(s) 271, 283
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 145
SetI ASST 3 cut(s) 102, 138, 305
SfuI TTCGAA 1 cut(s) 219
Sse9I AATT 3 cut(s) 39, 203, 277
SsiI CCGC 3 cut(s) 80, 140, 257
StyD4I CCNGG 1 cut(s) 143
TaiI ACGT 1 cut(s) 138
TaqI TCGA 1 cut(s) 219
TasI AATT 3 cut(s) 39, 203, 277
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 260
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 85
TspGWI ACGGA 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.