pycom16g03790

NIM1-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008703
Physical Location & Seq
Forward (+)
2345912 .. 2346226
315 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03790.1

Sequence Viewer

Length: 315 bp
ATGAATGCGGATAAGCGGAAGCGCGATGACAGAGGCGAATTAGAGGGGAAGAGAGCGAGAGCGGACGGGGGGGAACGTAACGGAGGCCACGAGATGGTAACGGAGGAGGAGGTGGAGGAGTTCTTTGCGATACTCAGGCGGATACATGTGGCAGCCAAGCACTTGGGCAGAGCGGCGTCCATGGAATGGAGGCCCTCATTCCAAGTTGAAGATTTTAAGGAAGAGAACGAGTCCCTTACGGTAAAAGACCCAAAAAAGGAGGAGAAACAAGCGGAGGCGGATTCGGGTTTGGATCTTAACTCACATCCCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

105

Amino Acids

11.87

Weight (kDa)

5.28

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 94, 186
AccBSI CCGCTC 2 cut(s) 62, 173
AccII CGCG 1 cut(s) 24
AciI CCGC 7 cut(s) 8, 16, 62, 139, 173, 272, 278
AclWI GGATC 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 176
AfiI CCNNNNNNNGG 3 cut(s) 94, 186, 256
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 1 cut(s) 209
AlwI GGATC 1 cut(s) 300
AoxI GGCC 2 cut(s) 85, 191
ApeKI GCWGC 1 cut(s) 152
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 192
BauI CACGAG 1 cut(s) 89
BbvI GCAGC 1 cut(s) 164
BccI CCATC 1 cut(s) 88
BciVI GTATCC 1 cut(s) 135
BfuI GTATCC 1 cut(s) 135
BisI GCNGC 2 cut(s) 153, 174
BlsI GCNGC 2 cut(s) 154, 175
BmgT120I GGNCC 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 180
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 186, 256
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 3 cut(s) 94, 186, 256
BseMII CTCAG 1 cut(s) 148
BseRI GAGGAG 4 cut(s) 119, 122, 131, 275
BseXI GCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 87, 193
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 3 cut(s) 94, 186, 256
BsmFI GGGAC 1 cut(s) 217
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 87, 193
Bsp143I GATC 1 cut(s) 292
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 7 cut(s) 8, 16, 62, 139, 173, 272, 278
BspANI GGCC 2 cut(s) 87, 193
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 1 cut(s) 24
BspPI GGATC 1 cut(s) 300
BsrBI CCGCTC 2 cut(s) 62, 173
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 1 cut(s) 292
BssNI GRCGYC 1 cut(s) 176
BssSI CACGAG 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 180
Bst2BI CACGAG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 2 cut(s) 44, 216
BstACI GRCGYC 1 cut(s) 176
BstDEI CTNAG 1 cut(s) 134
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstNSI RCATGY 1 cut(s) 149
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 292
BstXI CCANNNNNNTGG 1 cut(s) 163
BstYI RGATCY 1 cut(s) 292
BsuI GTATCC 1 cut(s) 135
BsuRI GGCC 2 cut(s) 87, 193
BtgI CCRYGG 1 cut(s) 180
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 304
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 192
CseI GACGC 1 cut(s) 165
CviAII CATG 2 cut(s) 146, 181
CviJI RGCY 3 cut(s) 87, 155, 193
CviKI_1 RGCY 3 cut(s) 87, 155, 193
DdeI CTNAG 1 cut(s) 134
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 44, 216
EarI CTCTTC 2 cut(s) 44, 216
EciI GGCGGA 2 cut(s) 154, 293
Eco130I CCWWGG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 2 cut(s) 149, 184
FaiI YATR 3 cut(s) 147, 182, 313
FaqI GGGAC 1 cut(s) 217
FatI CATG 2 cut(s) 145, 180
Fnu4HI GCNGC 2 cut(s) 153, 174
FokI GGATG 1 cut(s) 291
Fsp4HI GCNGC 2 cut(s) 153, 174
GlaI GCGC 1 cut(s) 23
GluI GCNGC 2 cut(s) 153, 174
HaeIII GGCC 2 cut(s) 87, 193
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 2 cut(s) 149, 184
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HinfI GANTC 2 cut(s) 230, 281
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4IV ACGT 1 cut(s) 76
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 76
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 2 cut(s) 149, 184
HspAI GCGC 1 cut(s) 22
Kzo9I GATC 1 cut(s) 292
LpnPI CCDG 1 cut(s) 121
Lsp1109I GCAGC 1 cut(s) 164
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 2 cut(s) 77, 97
MalI GATC 1 cut(s) 294
MbiI CCGCTC 2 cut(s) 62, 173
MboI GATC 1 cut(s) 292
MboII GAAGA 3 cut(s) 61, 221, 233
MflI RGATCY 1 cut(s) 292
MluCI AATT 1 cut(s) 38
MlyI GAGTC 1 cut(s) 239
MseI TTAA 2 cut(s) 216, 297
Mva1269I GAATGC 1 cut(s) 10
MvnI CGCG 1 cut(s) 24
NcoI CCATGG 1 cut(s) 180
NdeII GATC 1 cut(s) 292
NlaIII CATG 2 cut(s) 149, 184
NspI RCATGY 1 cut(s) 149
PciI ACATGT 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 1 cut(s) 281
PflMI CCANNNNNTGG 2 cut(s) 94, 186
PkrI GCNGC 2 cut(s) 154, 175
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PscI ACATGT 1 cut(s) 145
PspPI GGNCC 1 cut(s) 192
PsuI RGATCY 1 cut(s) 292
SaqAI TTAA 2 cut(s) 216, 297
SatI GCNGC 2 cut(s) 153, 174
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 1 cut(s) 192
SchI GAGTC 1 cut(s) 239
SetI ASST 2 cut(s) 79, 114
Sse9I AATT 1 cut(s) 38
SsiI CCGC 7 cut(s) 8, 16, 62, 139, 173, 272, 278
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 241
TaiI ACGT 1 cut(s) 79
TasI AATT 1 cut(s) 38
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 2 cut(s) 216, 297
Tru9I TTAA 2 cut(s) 216, 297
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 96, 116
Van91I CCANNNNNTGG 2 cut(s) 94, 186
XceI RCATGY 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.