FvH4_4g31480

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005812
Physical Location & Seq
Forward (+)
30595333 .. 30599113
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31480.t10

Sequence Viewer

Length: 1389 bp
ATGAGAAGAGGCGTGATCGGCGCGGTTCTCAAGTATCTTGGTCCAAGTGAGCATGATACTCCTCGCCTTGTTCGTCGAGGAAGAAGGTTCAGTCGCATTTCCATACTCCAATTCTCGCTCTGGGTTTTTGTCTTTGCTGCTATCTTTCTTCTCTATTTCCTCTTCTTCGGCCTCGTAATGTTATCTCCAGCTAAGATTGAAGAAAGTGCTGATGTTTCAGTACACGAAGAATCTCTGCCGTTTGATGGTGTGAATATTTCGGTAACGAAAGAATCTCCGCCCGCTGATGGTAAAAGCAAGCATCGTAAGAAATATGTTCCTTGTGAAATCGGGTTAAAAGAATCAGTTGATTTTATTAAGGAACCCAGGGAAGTTATGAACTTTACGCAGTTTTCGCTAGAGTACATTGACCAAGAGGAAAGGCTTTCTAGTAGTGGTGTATATGAACCTAGTTTTGGAGGGCATCCGACCCTTGAGGAAAGAGAGCGGTCTTTTTATGCCGTGAATCAAACTCTTCATTGCGGATTTGTCAGAGGGCCTGCAGCGTCCTCGAGCACTGGATTTGATATAGATGCGAAGGACAAGGCATATATGAATGGTTGTAAAGTTGCAGTATCTTCTTGCATTTTTGGAAGTTCTGACTTTTTGAGAAGGCCTACGAGTAGAAAGATCAGTGACTATTCCAAGAAAAATGTATGTTTTGTAATGTTTTTGGATGAAGAAACCCTGGCAAAAGTAACATCAGAGGGGCATGTTCCTGATGATACGGGACATATTGGCTTGTGGAAGATAGTTATAGTGAAGAACTTACCATATGCAGATATGCGCAGAACTGGAAAGGTGCCTAAACTTTTATCACATCGCCTTTTTCCCTCATCATGGTATTCCATTTGGCTTGACAGCAAGCTGCGACTTAATACTGATCCGATGCAGATTATCGAACACTTTTTATGGCGAGCAAAATCAGAATTTGCCATTTCCAATCACTATGACCGGCATTGTGTTTGGGAGGAGGTGCTCCAAAATAAGCGTCTAAATAAATATAATCACAAAATGATTGATGAGCAGTTCAGATTTTACCAGTCTGATGGCCTCGTCATGTTTAACTCCTCAGACCCAAATACTCCTCTTCCAAGTTATGTTCCCGAAGGTTCATTTATAGTCCGTGCACATACGCCTATGTCAAATTTATTTTCATGCCTTTGGTTTAATGAAGTTGACCGTTTTACCTCCCGCGATCAGCTAAGCTTTGCATATACTTTCTTGAAATTGAGGAGAATGAACCCAGATAGACCATTCTACCTAAATATGTTCAAGGACTGTGAGCGCAGAGCATTAACAAAGCTATTCCGGCATAGGGCAGAGCTATCTACCCCACCCGTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

463

Amino Acids

53.5

Weight (kDa)

8.92

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 133 - 452 6.9e-143 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 827
AccB1I GGYRCC 1 cut(s) 841
AccBSI CCGCTC 1 cut(s) 487
AccII CGCG 2 cut(s) 23, 1236
AciI CCGC 6 cut(s) 23, 278, 282, 487, 522, 1234
AclWI GGATC 1 cut(s) 917
AcsI RAATTY 2 cut(s) 968, 1186
AfaI GTAC 2 cut(s) 222, 404
AfiI CCNNNNNNNGG 5 cut(s) 245, 287, 455, 879, 1357
AgsI TTSAA 3 cut(s) 200, 1267, 1315
AjnI CCWGG 2 cut(s) 365, 726
AjuI GAANNNNNNNTTGG 2 cut(s) 438, 470
AluBI AGCT 6 cut(s) 191, 907, 1243, 1248, 1345, 1366
AluI AGCT 6 cut(s) 191, 907, 1243, 1248, 1345, 1366
Alw21I GWGCWC 3 cut(s) 557, 1020, 1171
Alw44I GTGCAC 1 cut(s) 1167
AlwI GGATC 1 cut(s) 917
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 4 cut(s) 169, 536, 653, 1090
ApaLI GTGCAC 1 cut(s) 1167
ApeKI GCWGC 3 cut(s) 137, 542, 907
ApoI RAATTY 2 cut(s) 968, 1186
ArsI GACNNNNNNTTYG 2 cut(s) 1015, 1047
AspLEI GCGC 3 cut(s) 23, 828, 1329
AspS9I GGNCC 2 cut(s) 41, 536
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 1171
BanI GGYRCC 1 cut(s) 841
Bbv12I GWGCWC 3 cut(s) 557, 1020, 1171
BbvI GCAGC 3 cut(s) 124, 554, 894
BccI CCATC 3 cut(s) 239, 281, 1082
BceAI ACGGC 2 cut(s) 223, 485
BcgI CGANNNNNNTGC 2 cut(s) 531, 565
BciT130I CCWGG 2 cut(s) 367, 728
BfaI CTAG 3 cut(s) 398, 429, 450
BfmI CTRYAG 1 cut(s) 540
BisI GCNGC 3 cut(s) 138, 543, 908
BlpI GCTNAGC 1 cut(s) 1244
BlsI GCNGC 3 cut(s) 139, 544, 909
Bme1390I CCNGG 2 cut(s) 367, 728
Bme18I GGWCC 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 2 cut(s) 41, 536
BmiI GGNNCC 2 cut(s) 363, 843
BmrFI CCNGG 2 cut(s) 367, 728
BmsI GCATC 4 cut(s) 310, 472, 562, 918
BpmI CTGGAG 1 cut(s) 171
Bpu1102I GCTNAGC 1 cut(s) 1244
BpuEI CTTGAG 2 cut(s) 14, 494
BsaJI CCNNGG 3 cut(s) 365, 366, 726
Bsc4I CCNNNNNNNGG 5 cut(s) 245, 287, 455, 879, 1357
Bse118I RCCGGY 1 cut(s) 993
Bse1I ACTGG 3 cut(s) 562, 838, 1081
Bse3DI GCAATG 1 cut(s) 517
BseBI CCWGG 2 cut(s) 367, 728
BseDI CCNNGG 3 cut(s) 365, 366, 726
BseGI GGATG 2 cut(s) 463, 721
BseLI CCNNNNNNNGG 5 cut(s) 245, 287, 455, 879, 1357
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 1 cut(s) 1125
BseNI ACTGG 3 cut(s) 562, 838, 1081
BseRI GAGGAG 5 cut(s) 51, 1025, 1099, 1116, 1288
BseSI GKGCMC 1 cut(s) 1171
BseXI GCAGC 3 cut(s) 124, 554, 894
Bsh1236I CGCG 2 cut(s) 23, 1236
BshFI GGCC 4 cut(s) 171, 538, 655, 1092
BshNI GGYRCC 1 cut(s) 841
BsiHKAI GWGCWC 3 cut(s) 557, 1020, 1171
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 2 cut(s) 994, 1351
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 5 cut(s) 245, 287, 455, 879, 1357
BsmFI GGGAC 1 cut(s) 783
BsnI GGCC 4 cut(s) 171, 538, 655, 1092
BsoBI CYCGRG 1 cut(s) 550
Bsp1286I GDGCHC 3 cut(s) 557, 1020, 1171
Bsp143I GATC 4 cut(s) 15, 669, 922, 1237
Bsp1720I GCTNAGC 1 cut(s) 1244
BspACI CCGC 6 cut(s) 23, 278, 282, 487, 522, 1234
BspANI GGCC 4 cut(s) 171, 538, 655, 1092
BspCNI CTCAG 1 cut(s) 1124
BspFNI CGCG 2 cut(s) 23, 1236
BspLI GGNNCC 2 cut(s) 363, 843
BspMAI CTGCAG 1 cut(s) 544
BspPI GGATC 1 cut(s) 917
BspT107I GGYRCC 1 cut(s) 841
BsrBI CCGCTC 1 cut(s) 487
BsrDI GCAATG 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 993
BsrI ACTGG 3 cut(s) 562, 838, 1081
BssAI RCCGGY 1 cut(s) 993
BssECI CCNNGG 3 cut(s) 365, 366, 726
BssMI GATC 4 cut(s) 15, 669, 922, 1237
Bst2UI CCWGG 2 cut(s) 367, 728
Bst4CI ACNGT 2 cut(s) 1223, 1322
Bst6I CTCTTC 3 cut(s) 167, 519, 1134
BstC8I GCNNGC 5 cut(s) 282, 299, 540, 905, 957
BstDEI CTNAG 3 cut(s) 192, 1111, 1244
BstF5I GGATG 2 cut(s) 463, 721
BstFNI CGCG 2 cut(s) 23, 1236
BstHHI GCGC 3 cut(s) 23, 828, 1329
BstKTI GATC 4 cut(s) 18, 672, 925, 1240
BstMBI GATC 4 cut(s) 15, 669, 922, 1237
BstMWI GCNNNNNNNGC 3 cut(s) 18, 394, 1351
BstNI CCWGG 2 cut(s) 367, 728
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 2 cut(s) 365, 726
BstSFI CTRYAG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 1171
BstUI CGCG 2 cut(s) 23, 1236
BstV1I GCAGC 3 cut(s) 124, 554, 894
BstXI CCANNNNNNTGG 1 cut(s) 1088
BsuRI GGCC 4 cut(s) 171, 538, 655, 1092
BtgZI GCGATG 1 cut(s) 845
BtsCI GGATG 2 cut(s) 463, 721
BtsIMutI CAGTG 2 cut(s) 555, 679
Cac8I GCNNGC 5 cut(s) 282, 299, 540, 905, 957
CfoI GCGC 3 cut(s) 23, 828, 1329
Cfr10I RCCGGY 1 cut(s) 993
Cfr13I GGNCC 2 cut(s) 41, 536
CseI GACGC 2 cut(s) 534, 1019
Csp6I GTAC 2 cut(s) 221, 403
CviAII CATG 5 cut(s) 53, 752, 879, 1099, 1197
CviQI GTAC 2 cut(s) 221, 403
DdeI CTNAG 3 cut(s) 192, 1111, 1244
DpnI GATC 4 cut(s) 17, 671, 924, 1239
DpnII GATC 4 cut(s) 15, 669, 922, 1237
Eam1104I CTCTTC 3 cut(s) 167, 519, 1134
EarI CTCTTC 3 cut(s) 167, 519, 1134
EciI GGCGGA 1 cut(s) 267
Eco147I AGGCCT 1 cut(s) 655
Eco47I GGWCC 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 365, 726
FaeI CATG 5 cut(s) 56, 755, 882, 1102, 1200
FaqI GGGAC 1 cut(s) 783
FatI CATG 5 cut(s) 52, 751, 878, 1098, 1196
FauI CCCGC 2 cut(s) 289, 1241
FauNDI CATATG 1 cut(s) 814
Fnu4HI GCNGC 3 cut(s) 138, 543, 908
FokI GGATG 2 cut(s) 450, 728
Fsp4HI GCNGC 3 cut(s) 138, 543, 908
FspBI CTAG 3 cut(s) 398, 429, 450
FspI TGCGCA 1 cut(s) 827
GlaI GCGC 3 cut(s) 22, 827, 1328
GluI GCNGC 3 cut(s) 138, 543, 908
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 4 cut(s) 171, 538, 655, 1092
HapII CCGG 2 cut(s) 994, 1351
HgaI GACGC 2 cut(s) 534, 1019
HhaI GCGC 3 cut(s) 23, 828, 1329
Hin1II CATG 5 cut(s) 56, 755, 882, 1102, 1200
Hin6I GCGC 3 cut(s) 21, 826, 1327
HinP1I GCGC 3 cut(s) 21, 826, 1327
HincII GTYRAC 1 cut(s) 1219
HindII GTYRAC 1 cut(s) 1219
HindIII AAGCTT 1 cut(s) 1246
HinfI GANTC 4 cut(s) 230, 272, 341, 505
HpaII CCGG 2 cut(s) 994, 1351
Hpy166II GTNNAC 3 cut(s) 223, 1169, 1219
Hpy188I TCNGA 9 cut(s) 468, 533, 640, 745, 927, 967, 1073, 1087, 1114
Hpy188III TCNNGA 3 cut(s) 758, 1145, 1264
Hpy8I GTNNAC 3 cut(s) 223, 1169, 1219
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 4 cut(s) 78, 571, 645, 1142
HpyCH4III ACNGT 2 cut(s) 1223, 1322
HpyCH4V TGCA 7 cut(s) 542, 611, 624, 818, 931, 1169, 1253
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 394, 1351
HpyF3I CTNAG 3 cut(s) 192, 1111, 1244
Hsp92II CATG 5 cut(s) 56, 755, 882, 1102, 1200
HspAI GCGC 3 cut(s) 21, 826, 1327
Kzo9I GATC 4 cut(s) 15, 669, 922, 1237
LmnI GCTCC 1 cut(s) 1023
Lsp1109I GCAGC 3 cut(s) 124, 554, 894
LweI GCATC 4 cut(s) 310, 472, 562, 918
MaeI CTAG 3 cut(s) 398, 429, 450
MaeIII GTNAC 3 cut(s) 262, 674, 736
MalI GATC 4 cut(s) 17, 671, 924, 1239
MbiI CCGCTC 1 cut(s) 487
MboI GATC 4 cut(s) 15, 669, 922, 1237
MhlI GDGCHC 3 cut(s) 557, 1020, 1171
MluCI AATT 4 cut(s) 110, 968, 1186, 1268
MmeI TCCRAC 1 cut(s) 491
MseI TTAA 7 cut(s) 335, 357, 915, 1104, 1209, 1337, 1387
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 2 cut(s) 994, 1351
MspR9I CCNGG 2 cut(s) 367, 728
MvaI CCWGG 2 cut(s) 367, 728
MvnI CGCG 2 cut(s) 23, 1236
MwoI GCNNNNNNNGC 3 cut(s) 18, 394, 1351
NdeI CATATG 1 cut(s) 814
NdeII GATC 4 cut(s) 15, 669, 922, 1237
NlaIII CATG 5 cut(s) 56, 755, 882, 1102, 1200
NlaIV GGNNCC 2 cut(s) 363, 843
NmuCI GTSAC 1 cut(s) 674
NsbI TGCGCA 1 cut(s) 827
NspI RCATGY 1 cut(s) 755
PaeR7I CTCGAG 1 cut(s) 550
PasI CCCWGGG 1 cut(s) 366
PceI AGGCCT 1 cut(s) 655
PcsI WCGNNNNNNNCGW 1 cut(s) 70
PfeI GAWTC 4 cut(s) 230, 272, 341, 505
PkrI GCNGC 3 cut(s) 139, 544, 909
Psp6I CCWGG 2 cut(s) 365, 726
PspGI CCWGG 2 cut(s) 365, 726
PspN4I GGNNCC 2 cut(s) 363, 843
PspPI GGNCC 2 cut(s) 41, 536
PspXI VCTCGAGB 1 cut(s) 550
PstI CTGCAG 1 cut(s) 544
RsaI GTAC 2 cut(s) 222, 404
RsaNI GTAC 2 cut(s) 221, 403
SaqAI TTAA 7 cut(s) 335, 357, 915, 1104, 1209, 1337, 1387
SatI GCNGC 3 cut(s) 138, 543, 908
Sau3AI GATC 4 cut(s) 15, 669, 922, 1237
Sau96I GGNCC 2 cut(s) 41, 536
ScrFI CCNGG 2 cut(s) 367, 728
SduI GDGCHC 3 cut(s) 557, 1020, 1171
SfaNI GCATC 4 cut(s) 310, 472, 562, 918
SfcI CTRYAG 1 cut(s) 540
Sfr274I CTCGAG 1 cut(s) 550
SinI GGWCC 1 cut(s) 41
SlaI CTCGAG 1 cut(s) 550
SmlI CTYRAG 3 cut(s) 29, 473, 550
SmoI CTYRAG 3 cut(s) 29, 473, 550
Sse9I AATT 4 cut(s) 110, 968, 1186, 1268
SseBI AGGCCT 1 cut(s) 655
SsiI CCGC 6 cut(s) 23, 278, 282, 487, 522, 1234
SspI AATATT 1 cut(s) 256
SspMI CTAG 3 cut(s) 398, 429, 450
StuI AGGCCT 1 cut(s) 655
StyD4I CCNGG 2 cut(s) 365, 726
TaaI ACNGT 2 cut(s) 1223, 1322
TaqI TCGA 3 cut(s) 76, 551, 939
TasI AATT 4 cut(s) 110, 968, 1186, 1268
TatI WGTACW 2 cut(s) 220, 402
TfiI GAWTC 4 cut(s) 230, 272, 341, 505
Tru1I TTAA 7 cut(s) 335, 357, 915, 1104, 1209, 1337, 1387
Tru9I TTAA 7 cut(s) 335, 357, 915, 1104, 1209, 1337, 1387
TscAI CASTG 2 cut(s) 562, 679
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 3 cut(s) 137, 542, 907
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 9 cut(s) 392, 459, 506, 608, 732, 1143, 1185, 1227, 1295
TspGWI ACGGA 1 cut(s) 1154
TspRI CASTG 2 cut(s) 562, 679
VneI GTGCAC 1 cut(s) 1167
VpaK11BI GGWCC 1 cut(s) 41
XapI RAATTY 2 cut(s) 968, 1186
XceI RCATGY 1 cut(s) 755
XhoI CTCGAG 1 cut(s) 550
XspI CTAG 3 cut(s) 398, 429, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.