RLG00000006219

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005812
Physical Location & Seq
Reverse (-)
5320014 .. 5323024
3011 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006219

Sequence Viewer

Length: 1335 bp
ATGGGAAGAGGTCTGATGGGAGCGGTTCTGCTGTATCTTGGTCCAAGTGAGCATGGTACTCCTCGCTTTGTTCGCAGAGGAAGAAGGTTTAGTCGCATTTCTACACTTCAATGGGTTTTTGTCTTTGCAGCAATCTTTCTGCTTTATTTCATCTTCTTCGGCCTCACAATGTTGTCTACAGATGAGATTGAAGAAAGTGCAGATATTTCAGTAAAGGTAGAATCTTCGCGGACTGATGGTGGAAGCAAGCATCGTAGGAAATATACTCCTTGCGGCGTTCCGTTGAAGGAATCAGTTGATTATTTTAAGGAACCCCGGGAAGTTATGAACTTTACACAGTTTTCACTAGAGTACATTGACCAAGAGGAAAAACTTCCTACAAGTGATATATATGAACCTAGATTTGGAGGGCATCCGACCCTTGAGGAAAGAGAGCAGTCCTTTTATGCCGTAAATCAAACTCTTCATTGCGGATTTGTCAATGGGCCTTCAGGGTCCTCAAGCACTGGATTTGATTTAGATATGAAGGACAAGGCGTATATGAAGGATTGTAAGGTTGCAGTATCTTCTTGCATTTTTGGGAGCTCTGATTTTCTAAGAAGGCCTACAAGTAGAAAGATCAGTGACTATTCCAAGAAAAATGTTTGTTTTGTAATGTTTGTGGATGAAGAAAGCCTGGCAAAAATATCATCCGAGGGGCATTTTCCTGATGATACAGGACATATTGGCTTGTGGAGGATAGTTATAGTGAAGAACTTACCATATGCAGATATGCGGAGAACTGGAAAGGTGCCAAAACTTTTATCACATCGCCTTTTTCCCTCATCATGGTATTCCATTTGGGTTGACAGCAAGATGCGACTTAATACTGATCCGATGCAGATTATCGAATACTTTTTATGGCGAGCAAAATCAGAATATGCCATTTCCAATCACTATGACCGGCATTGTGTTTGGGAGGAGGTGCTTCAAAATAAGCGTCTAAATAAGTACAATCACACAGTGATTGATGAGCAGTTCACATTTTACCAATCTGATGGCCTCACCAAGTTTGACTCTTCAGACCCAAATACTCCTCTTCCAAGTTATGTTCCCGAAGGTTCATTTATTGTCCGTGCACATACACCTATTTCAAATTTATTTTCATGCCTCTGGTTTAATGAAGTTGACCGGTTTACCTCCCGCGATCAGCTAAGCTTTGCATATACTTTCTTAAAATTGAGGAGAATGAACCCAGATAGACCATTCTACCTAAATATGTTCAAGGACTGTGAGCGCAGAGCACTAGCAAAGCTATTCCGGCACAGGGCAGAGCTGTCTCCACCTGTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

445

Amino Acids

51.64

Weight (kDa)

8.61

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 116 - 435 9.4e-144 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 176
AccII CGCG 2 cut(s) 229, 1185
AciI CCGC 6 cut(s) 23, 229, 273, 471, 775, 1183
AclWI GGATC 1 cut(s) 866
AcsI RAATTY 1 cut(s) 1135
AcuI CTGAAG 2 cut(s) 474, 1044
AdeI CACNNNGTG 1 cut(s) 1003
AfaI GTAC 3 cut(s) 58, 353, 992
AfiI CCNNNNNNNGG 3 cut(s) 404, 828, 1306
AgeI ACCGGT 1 cut(s) 1170
AgsI TTSAA 6 cut(s) 110, 191, 286, 971, 1134, 1264
AjnI CCWGG 1 cut(s) 675
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 5 cut(s) 585, 1192, 1197, 1294, 1315
AluI AGCT 5 cut(s) 585, 1192, 1197, 1294, 1315
Alw21I GWGCWC 3 cut(s) 587, 1120, 1285
Alw26I GTCTC 1 cut(s) 1323
Alw44I GTGCAC 1 cut(s) 1116
AlwI GGATC 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 315
AoxI GGCC 4 cut(s) 160, 485, 602, 1039
ApaLI GTGCAC 1 cut(s) 1116
ApeKI GCWGC 1 cut(s) 128
ApoI RAATTY 1 cut(s) 1135
ArsI GACNNNNNNTTYG 2 cut(s) 964, 996
AsiGI ACCGGT 1 cut(s) 1170
Asp700I GAANNNNTTC 1 cut(s) 372
AspLEI GCGC 1 cut(s) 1278
AspS9I GGNCC 3 cut(s) 41, 485, 495
AsuC2I CCSGG 2 cut(s) 316, 317
AsuHPI GGTGA 1 cut(s) 1036
AvaI CYCGRG 1 cut(s) 315
AvaII GGWCC 2 cut(s) 41, 495
BaeGI GKGCMC 1 cut(s) 1120
BanI GGYRCC 1 cut(s) 790
BanII GRGCYC 1 cut(s) 587
Bbv12I GWGCWC 3 cut(s) 587, 1120, 1285
BbvI GCAGC 1 cut(s) 140
BccI CCATC 3 cut(s) 10, 230, 1031
BceAI ACGGC 1 cut(s) 434
BciT130I CCWGG 1 cut(s) 677
BcnI CCSGG 2 cut(s) 316, 317
BcoDI GTCTC 1 cut(s) 1323
BfaI CTAG 3 cut(s) 347, 399, 1286
BfmI CTRYAG 1 cut(s) 177
BisI GCNGC 2 cut(s) 129, 274
BlpI GCTNAGC 1 cut(s) 1193
BlsI GCNGC 2 cut(s) 130, 275
Bme1390I CCNGG 3 cut(s) 316, 317, 677
Bme18I GGWCC 2 cut(s) 41, 495
BmeT110I CYCGRG 1 cut(s) 315
BmgT120I GGNCC 3 cut(s) 41, 485, 495
BmiI GGNNCC 3 cut(s) 312, 496, 792
BmrFI CCNGG 3 cut(s) 316, 317, 677
BmsI GCATC 4 cut(s) 259, 421, 846, 867
Bpu1102I GCTNAGC 1 cut(s) 1193
BpuEI CTTGAG 2 cut(s) 443, 484
BpuMI CCSGG 2 cut(s) 316, 317
BsaJI CCNNGG 3 cut(s) 314, 315, 693
BsaWI WCCGGW 1 cut(s) 1170
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 828, 1306
Bse118I RCCGGY 2 cut(s) 942, 1170
Bse1I ACTGG 2 cut(s) 511, 787
Bse3DI GCAATG 1 cut(s) 466
BseBI CCWGG 1 cut(s) 677
BseDI CCNNGG 3 cut(s) 314, 315, 693
BseGI GGATG 3 cut(s) 412, 670, 689
BseLI CCNNNNNNNGG 3 cut(s) 404, 828, 1306
BseMI GCAATG 1 cut(s) 466
BseNI ACTGG 2 cut(s) 511, 787
BseRI GAGGAG 4 cut(s) 51, 974, 1065, 1237
BseSI GKGCMC 1 cut(s) 1120
BseXI GCAGC 1 cut(s) 140
BsgI GTGCAG 1 cut(s) 219
Bsh1236I CGCG 2 cut(s) 229, 1185
BshFI GGCC 4 cut(s) 162, 487, 604, 1041
BshNI GGYRCC 1 cut(s) 790
BshTI ACCGGT 1 cut(s) 1170
BsiHKAI GWGCWC 3 cut(s) 587, 1120, 1285
BsiHKCI CYCGRG 1 cut(s) 315
BsiSI CCGG 4 cut(s) 316, 943, 1171, 1300
BslI CCNNNNNNNGG 3 cut(s) 404, 828, 1306
BsmAI GTCTC 1 cut(s) 1323
BsnI GGCC 4 cut(s) 162, 487, 604, 1041
BsoBI CYCGRG 1 cut(s) 315
Bsp1286I GDGCHC 3 cut(s) 587, 1120, 1285
Bsp143I GATC 3 cut(s) 618, 871, 1186
Bsp1720I GCTNAGC 1 cut(s) 1193
BspACI CCGC 6 cut(s) 23, 229, 273, 471, 775, 1183
BspANI GGCC 4 cut(s) 162, 487, 604, 1041
BspFNI CGCG 2 cut(s) 229, 1185
BspLI GGNNCC 3 cut(s) 312, 496, 792
BspPI GGATC 1 cut(s) 866
BspT107I GGYRCC 1 cut(s) 790
BsrBI CCGCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 466
BsrFI RCCGGY 2 cut(s) 942, 1170
BsrI ACTGG 2 cut(s) 511, 787
BssAI RCCGGY 2 cut(s) 942, 1170
BssECI CCNNGG 3 cut(s) 314, 315, 693
BssMI GATC 3 cut(s) 618, 871, 1186
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 3 cut(s) 339, 1003, 1271
Bst6I CTCTTC 3 cut(s) 468, 1063, 1083
BstC8I GCNNGC 2 cut(s) 248, 906
BstDEI CTNAG 2 cut(s) 596, 1193
BstF5I GGATG 3 cut(s) 412, 670, 689
BstFNI CGCG 2 cut(s) 229, 1185
BstHHI GCGC 1 cut(s) 1278
BstKTI GATC 3 cut(s) 621, 874, 1189
BstMAI GTCTC 1 cut(s) 1323
BstMBI GATC 3 cut(s) 618, 871, 1186
BstMWI GCNNNNNNNGC 2 cut(s) 72, 1300
BstNI CCWGG 1 cut(s) 677
BstSCI CCNGG 3 cut(s) 314, 315, 675
BstSFI CTRYAG 1 cut(s) 177
BstSLI GKGCMC 1 cut(s) 1120
BstUI CGCG 2 cut(s) 229, 1185
BstV1I GCAGC 1 cut(s) 140
BstXI CCANNNNNNTGG 1 cut(s) 1037
BsuRI GGCC 4 cut(s) 162, 487, 604, 1041
BtgZI GCGATG 1 cut(s) 794
BtsCI GGATG 3 cut(s) 412, 670, 689
BtsIMutI CAGTG 3 cut(s) 504, 628, 1008
Cac8I GCNNGC 2 cut(s) 248, 906
CfoI GCGC 1 cut(s) 1278
Cfr10I RCCGGY 2 cut(s) 942, 1170
Cfr13I GGNCC 3 cut(s) 41, 485, 495
Cfr9I CCCGGG 1 cut(s) 315
CseI GACGC 1 cut(s) 968
Csp6I GTAC 3 cut(s) 57, 352, 991
CspAI ACCGGT 1 cut(s) 1170
CviAII CATG 3 cut(s) 53, 828, 1146
CviQI GTAC 3 cut(s) 57, 352, 991
DdeI CTNAG 2 cut(s) 596, 1193
DpnI GATC 3 cut(s) 620, 873, 1188
DpnII GATC 3 cut(s) 618, 871, 1186
DraIII CACNNNGTG 1 cut(s) 1003
Eam1104I CTCTTC 3 cut(s) 468, 1063, 1083
EarI CTCTTC 3 cut(s) 468, 1063, 1083
Ecl136II GAGCTC 1 cut(s) 585
Eco147I AGGCCT 1 cut(s) 604
Eco24I GRGCYC 1 cut(s) 587
Eco47I GGWCC 2 cut(s) 41, 495
Eco53kI GAGCTC 1 cut(s) 585
Eco57I CTGAAG 2 cut(s) 474, 1044
Eco88I CYCGRG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 585
EcoO109I RGGNCCY 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 675
EcoT38I GRGCYC 1 cut(s) 587
FaeI CATG 3 cut(s) 56, 831, 1149
FatI CATG 3 cut(s) 52, 827, 1145
FauI CCCGC 1 cut(s) 1190
FauNDI CATATG 1 cut(s) 763
FblI GTMKAC 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 129, 274
FokI GGATG 3 cut(s) 399, 676, 677
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 2 cut(s) 129, 274
FspBI CTAG 3 cut(s) 347, 399, 1286
GlaI GCGC 1 cut(s) 1277
GluI GCNGC 2 cut(s) 129, 274
HaeIII GGCC 4 cut(s) 162, 487, 604, 1041
HapII CCGG 4 cut(s) 316, 943, 1171, 1300
HgaI GACGC 1 cut(s) 968
HhaI GCGC 1 cut(s) 1278
Hin1II CATG 3 cut(s) 56, 831, 1149
Hin6I GCGC 1 cut(s) 1276
HinP1I GCGC 1 cut(s) 1276
HincII GTYRAC 2 cut(s) 847, 1168
HindII GTYRAC 2 cut(s) 847, 1168
HindIII AAGCTT 1 cut(s) 1195
HinfI GANTC 3 cut(s) 221, 290, 1055
HpaII CCGG 4 cut(s) 316, 943, 1171, 1300
HphI GGTGA 1 cut(s) 1036
Hpy166II GTNNAC 6 cut(s) 177, 847, 1020, 1118, 1168, 1176
Hpy188I TCNGA 8 cut(s) 15, 417, 589, 694, 876, 916, 1036, 1063
Hpy188III TCNNGA 2 cut(s) 707, 1094
Hpy8I GTNNAC 6 cut(s) 177, 847, 1020, 1118, 1168, 1176
HpyAV CCTTC 7 cut(s) 78, 280, 498, 520, 538, 594, 1091
HpyCH4III ACNGT 3 cut(s) 339, 1003, 1271
HpyCH4V TGCA 8 cut(s) 128, 200, 560, 573, 767, 880, 1118, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 1300
HpyF3I CTNAG 2 cut(s) 596, 1193
Hsp92II CATG 3 cut(s) 56, 831, 1149
HspAI GCGC 1 cut(s) 1276
Kzo9I GATC 3 cut(s) 618, 871, 1186
LmnI GCTCC 2 cut(s) 20, 582
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 4 cut(s) 259, 421, 846, 867
MaeI CTAG 3 cut(s) 347, 399, 1286
MaeIII GTNAC 2 cut(s) 623, 1327
MalI GATC 3 cut(s) 620, 873, 1188
MbiI CCGCTC 1 cut(s) 23
MboI GATC 3 cut(s) 618, 871, 1186
MhlI GDGCHC 3 cut(s) 587, 1120, 1285
MluCI AATT 2 cut(s) 1135, 1217
MlyI GAGTC 1 cut(s) 1049
MmeI TCCRAC 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 372
MseI TTAA 4 cut(s) 306, 864, 1158, 1214
MslI CAYNNNNRTG 1 cut(s) 109
MspI CCGG 4 cut(s) 316, 943, 1171, 1300
MspR9I CCNGG 3 cut(s) 316, 317, 677
MvaI CCWGG 1 cut(s) 677
MvnI CGCG 2 cut(s) 229, 1185
MwoI GCNNNNNNNGC 2 cut(s) 72, 1300
NciI CCSGG 2 cut(s) 316, 317
NdeI CATATG 1 cut(s) 763
NdeII GATC 3 cut(s) 618, 871, 1186
NlaIII CATG 3 cut(s) 56, 831, 1149
NlaIV GGNNCC 3 cut(s) 312, 496, 792
NmuCI GTSAC 1 cut(s) 623
PceI AGGCCT 1 cut(s) 604
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 221, 290
PinAI ACCGGT 1 cut(s) 1170
PkrI GCNGC 2 cut(s) 130, 275
PleI GAGTC 1 cut(s) 1049
PpsI GAGTC 1 cut(s) 1049
PpuMI RGGWCCY 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 675
PspGI CCWGG 1 cut(s) 675
PspN4I GGNNCC 3 cut(s) 312, 496, 792
PspPI GGNCC 3 cut(s) 41, 485, 495
PspPPI RGGWCCY 1 cut(s) 495
RsaI GTAC 3 cut(s) 58, 353, 992
RsaNI GTAC 3 cut(s) 57, 352, 991
RseI CAYNNNNRTG 1 cut(s) 109
SacI GAGCTC 1 cut(s) 587
SaqAI TTAA 4 cut(s) 306, 864, 1158, 1214
SatI GCNGC 2 cut(s) 129, 274
Sau3AI GATC 3 cut(s) 618, 871, 1186
Sau96I GGNCC 3 cut(s) 41, 485, 495
SchI GAGTC 1 cut(s) 1049
ScrFI CCNGG 3 cut(s) 316, 317, 677
SduI GDGCHC 3 cut(s) 587, 1120, 1285
SfaNI GCATC 4 cut(s) 259, 421, 846, 867
SfcI CTRYAG 1 cut(s) 177
SinI GGWCC 2 cut(s) 41, 495
SmaI CCCGGG 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 109
SmlI CTYRAG 2 cut(s) 422, 499
SmoI CTYRAG 2 cut(s) 422, 499
Sse9I AATT 2 cut(s) 1135, 1217
SseBI AGGCCT 1 cut(s) 604
SsiI CCGC 6 cut(s) 23, 229, 273, 471, 775, 1183
SspMI CTAG 3 cut(s) 347, 399, 1286
SstI GAGCTC 1 cut(s) 587
StuI AGGCCT 1 cut(s) 604
StyD4I CCNGG 3 cut(s) 314, 315, 675
TaaI ACNGT 3 cut(s) 339, 1003, 1271
TaqI TCGA 1 cut(s) 888
TasI AATT 2 cut(s) 1135, 1217
TatI WGTACW 2 cut(s) 351, 990
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 2 cut(s) 221, 290
Tru1I TTAA 4 cut(s) 306, 864, 1158, 1214
Tru9I TTAA 4 cut(s) 306, 864, 1158, 1214
TscAI CASTG 3 cut(s) 511, 628, 1008
TseFI GTSAC 1 cut(s) 623
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 623
TspGWI ACGGA 2 cut(s) 270, 1103
TspMI CCCGGG 1 cut(s) 315
TspRI CASTG 3 cut(s) 511, 628, 1008
VneI GTGCAC 1 cut(s) 1116
VpaK11BI GGWCC 2 cut(s) 41, 495
XapI RAATTY 1 cut(s) 1135
XmaI CCCGGG 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 3 cut(s) 347, 399, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.