Rh4AG373900

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005812
Physical Location & Seq
Forward (+)
68141996 .. 68145883
3888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG373900.1

Sequence Viewer

Length: 1356 bp
ATGGGAAGAGGTCTGATGGGAGCGGTTCTGATGTATCTTGGTCCAAGTGAGCATGGTACTCCTCGCTTTGTTCGCAGAGGAAGAAGGTTTAGTCGCATTTCTACACTGCAATTCTCGCTCTGGGTTTTTGTCTTTGCAGCAATCTTTCTGCTTTATTTCATCTTCTTCGGCCTCACAATGTTGTCTACAGATGAGAATGAGAATGAAGAAAGTGCAGTTATTCCAGTAAAGGTAGAATCTTCGCGGACTGATGGTGGAAGCAAGCATCGTAGGAAATATACTCCTTGCGACGTTCCGTTGAAGGAATCAGTTGATTATTTTAAGGAACCCCGGGAAGTTATGAACTTTACACAGTTTTCACTAGAGTACATTGACCAAGAGGAAAAACTTCCTACAAGTAATATATATGAACCTAGATTTGGAGGGCATCCGACCCTTGAGGAAAGAGAGCAGTCCTTTTATGCCGTAAATCAAACTCTTCATTGCGGATTTGTCAACGGGCCTCCAGGGTCCTCAAGCACTGGGTTTGATTTAGATATGAAGGACAAGGCGTATATGAAGGATTGTAAGGTTGCAGTATCTTCTTGCATTTTTGGGAGCTCTGATTTTCTAAGAAGGCCTACAAGTAGAAAGATCAGTGACTATTCCAAGAAAAATGTTTGTTTTGTAATGTTTGTGGATGAAGAAAGCCTGGCAAAAATATCATCCGAGGGGCATTTTCGTGATGATACAGGACATATTGGCTTGTGGAGGATAGTTATAGTGAAGAACTTACCATATGCAGATATGCGGAGAACTGGAAAGGTGCCAAAACTTTTATCACATCGCCTTTTTCCCTCATCATGGTATTCCATTTGGGTTGACAGCAAGATGCGACTTAATACTGATCCGATGCAGATTATCGAATACTTTTTATGGCGAGCAAAATCAGAATATGCCATTTCCAATCACTATGACCGGCATTGTGTTTGGGAGGAGGTGCTCCAAAATAAGCGTCTAAATAAGTACAATCACACAGTGATTGATGAGCAGTTCACATTTTACCAATCTGATGGCCTCACCAAGTTTGACTCTTCAGACCCAAATACTCCTCTTCCAAGTTATGTTCCCGAAGGTTCATTTATTGTCCGTGCACATACACCTATTTCAAATTTATTTTCATGCCTCTGGTTTAATGAAGTTGACCGGTTTACCTCCCGCGATCAGCTTAGCTTTGCATATACTTTCTTAAAATTGAAGAGAATGAACCCAGATAGACCATTCTACCTAAATATGTTCAAGGTACTGGAAATTCTTAGAAAAATAAAAAATTTATTGCATATACTTCTGTTAGTTGTTAGATCATATTTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

451

Amino Acids

52.73

Weight (kDa)

8.84

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 121 - 431 2.1e-138 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 805
AccBSI CCGCTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 185
AccII CGCG 2 cut(s) 244, 1200
AciI CCGC 5 cut(s) 23, 244, 486, 790, 1198
AclWI GGATC 1 cut(s) 881
AcsI RAATTY 3 cut(s) 1150, 1290, 1309
AcuI CTGAAG 1 cut(s) 1059
AdeI CACNNNGTG 1 cut(s) 1018
AfaI GTAC 4 cut(s) 58, 368, 1007, 1284
AfiI CCNNNNNNNGG 2 cut(s) 419, 843
AgeI ACCGGT 1 cut(s) 1185
AgsI TTSAA 4 cut(s) 301, 1149, 1237, 1279
AjnI CCWGG 2 cut(s) 505, 690
AjuI GAANNNNNNNTTGG 2 cut(s) 402, 434
AluBI AGCT 3 cut(s) 600, 1207, 1212
AluI AGCT 3 cut(s) 600, 1207, 1212
Alw21I GWGCWC 3 cut(s) 602, 984, 1135
Alw44I GTGCAC 1 cut(s) 1131
AlwI GGATC 1 cut(s) 881
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 4 cut(s) 169, 500, 617, 1054
ApaLI GTGCAC 1 cut(s) 1131
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 3 cut(s) 1150, 1290, 1309
ArsI GACNNNNNNTTYG 2 cut(s) 979, 1011
AsiGI ACCGGT 1 cut(s) 1185
Asp700I GAANNNNTTC 1 cut(s) 387
AspS9I GGNCC 3 cut(s) 41, 500, 510
AsuC2I CCSGG 2 cut(s) 331, 332
AsuHPI GGTGA 1 cut(s) 1051
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 2 cut(s) 41, 510
BaeGI GKGCMC 1 cut(s) 1135
BanI GGYRCC 1 cut(s) 805
BanII GRGCYC 1 cut(s) 602
Bbv12I GWGCWC 3 cut(s) 602, 984, 1135
BbvI GCAGC 1 cut(s) 149
BccI CCATC 3 cut(s) 10, 245, 1046
BceAI ACGGC 1 cut(s) 449
BciT130I CCWGG 2 cut(s) 507, 692
BcnI CCSGG 2 cut(s) 331, 332
BfaI CTAG 2 cut(s) 362, 414
BfmI CTRYAG 1 cut(s) 186
BisI GCNGC 1 cut(s) 138
BlpI GCTNAGC 1 cut(s) 1208
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 4 cut(s) 331, 332, 507, 692
Bme18I GGWCC 2 cut(s) 41, 510
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 3 cut(s) 41, 500, 510
BmiI GGNNCC 3 cut(s) 327, 511, 807
BmrFI CCNGG 4 cut(s) 331, 332, 507, 692
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 4 cut(s) 274, 436, 861, 882
BmuI ACTGGG 1 cut(s) 531
BpmI CTGGAG 1 cut(s) 489
Bpu1102I GCTNAGC 1 cut(s) 1208
BpuEI CTTGAG 2 cut(s) 458, 499
BpuMI CCSGG 2 cut(s) 331, 332
BsaJI CCNNGG 4 cut(s) 329, 330, 506, 708
BsaWI WCCGGW 1 cut(s) 1185
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 843
Bse118I RCCGGY 2 cut(s) 957, 1185
Bse1I ACTGG 4 cut(s) 224, 526, 802, 1290
Bse3DI GCAATG 1 cut(s) 481
BseBI CCWGG 2 cut(s) 507, 692
BseDI CCNNGG 4 cut(s) 329, 330, 506, 708
BseGI GGATG 3 cut(s) 427, 685, 704
BseLI CCNNNNNNNGG 2 cut(s) 419, 843
BseMI GCAATG 1 cut(s) 481
BseNI ACTGG 4 cut(s) 224, 526, 802, 1290
BseRI GAGGAG 3 cut(s) 51, 989, 1080
BseSI GKGCMC 1 cut(s) 1135
BseXI GCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 234
Bsh1236I CGCG 2 cut(s) 244, 1200
BshFI GGCC 4 cut(s) 171, 502, 619, 1056
BshNI GGYRCC 1 cut(s) 805
BshTI ACCGGT 1 cut(s) 1185
BsiHKAI GWGCWC 3 cut(s) 602, 984, 1135
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 3 cut(s) 331, 958, 1186
BslI CCNNNNNNNGG 2 cut(s) 419, 843
BsnI GGCC 4 cut(s) 171, 502, 619, 1056
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 3 cut(s) 602, 984, 1135
Bsp143I GATC 4 cut(s) 633, 886, 1201, 1340
Bsp1720I GCTNAGC 1 cut(s) 1208
BspACI CCGC 5 cut(s) 23, 244, 486, 790, 1198
BspANI GGCC 4 cut(s) 171, 502, 619, 1056
BspFNI CGCG 2 cut(s) 244, 1200
BspLI GGNNCC 3 cut(s) 327, 511, 807
BspPI GGATC 1 cut(s) 881
BspT107I GGYRCC 1 cut(s) 805
BsrBI CCGCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 481
BsrFI RCCGGY 2 cut(s) 957, 1185
BsrI ACTGG 4 cut(s) 224, 526, 802, 1290
BssAI RCCGGY 2 cut(s) 957, 1185
BssECI CCNNGG 4 cut(s) 329, 330, 506, 708
BssMI GATC 4 cut(s) 633, 886, 1201, 1340
Bst2UI CCWGG 2 cut(s) 507, 692
Bst4CI ACNGT 2 cut(s) 354, 1018
Bst6I CTCTTC 4 cut(s) 483, 1078, 1098, 1232
BstC8I GCNNGC 2 cut(s) 263, 921
BstDEI CTNAG 3 cut(s) 611, 1208, 1295
BstF5I GGATG 3 cut(s) 427, 685, 704
BstFNI CGCG 2 cut(s) 244, 1200
BstKTI GATC 4 cut(s) 636, 889, 1204, 1343
BstMBI GATC 4 cut(s) 633, 886, 1201, 1340
BstMWI GCNNNNNNNGC 2 cut(s) 72, 115
BstNI CCWGG 2 cut(s) 507, 692
BstSCI CCNGG 4 cut(s) 329, 330, 505, 690
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 1135
BstUI CGCG 2 cut(s) 244, 1200
BstV1I GCAGC 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 1052
BsuRI GGCC 4 cut(s) 171, 502, 619, 1056
BtgZI GCGATG 1 cut(s) 809
BtsCI GGATG 3 cut(s) 427, 685, 704
BtsI GCAGTG 1 cut(s) 104
BtsIMutI CAGTG 4 cut(s) 104, 519, 643, 1023
Cac8I GCNNGC 2 cut(s) 263, 921
Cfr10I RCCGGY 2 cut(s) 957, 1185
Cfr13I GGNCC 3 cut(s) 41, 500, 510
Cfr9I CCCGGG 1 cut(s) 330
CseI GACGC 1 cut(s) 983
Csp6I GTAC 4 cut(s) 57, 367, 1006, 1283
CspAI ACCGGT 1 cut(s) 1185
CviAII CATG 3 cut(s) 53, 843, 1161
CviJI RGCY 9 cut(s) 171, 502, 600, 619, 690, 744, 1056, 1207, 1212
CviKI_1 RGCY 9 cut(s) 171, 502, 600, 619, 690, 744, 1056, 1207, 1212
CviQI GTAC 4 cut(s) 57, 367, 1006, 1283
DdeI CTNAG 3 cut(s) 611, 1208, 1295
DpnI GATC 4 cut(s) 635, 888, 1203, 1342
DpnII GATC 4 cut(s) 633, 886, 1201, 1340
DraIII CACNNNGTG 1 cut(s) 1018
Eam1104I CTCTTC 4 cut(s) 483, 1078, 1098, 1232
EarI CTCTTC 4 cut(s) 483, 1078, 1098, 1232
Ecl136II GAGCTC 1 cut(s) 600
Eco147I AGGCCT 1 cut(s) 619
Eco24I GRGCYC 1 cut(s) 602
Eco47I GGWCC 2 cut(s) 41, 510
Eco53kI GAGCTC 1 cut(s) 600
Eco57I CTGAAG 1 cut(s) 1059
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 505, 690
EcoT38I GRGCYC 1 cut(s) 602
FaeI CATG 3 cut(s) 56, 846, 1164
FatI CATG 3 cut(s) 52, 842, 1160
FauI CCCGC 1 cut(s) 1205
FauNDI CATATG 1 cut(s) 778
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 3 cut(s) 414, 691, 692
FriOI GRGCYC 1 cut(s) 602
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 2 cut(s) 362, 414
GluI GCNGC 1 cut(s) 138
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 4 cut(s) 171, 502, 619, 1056
HapII CCGG 3 cut(s) 331, 958, 1186
HgaI GACGC 1 cut(s) 983
Hin1II CATG 3 cut(s) 56, 846, 1164
HincII GTYRAC 3 cut(s) 496, 862, 1183
HindII GTYRAC 3 cut(s) 496, 862, 1183
HinfI GANTC 3 cut(s) 236, 305, 1070
HpaII CCGG 3 cut(s) 331, 958, 1186
HphI GGTGA 1 cut(s) 1051
Hpy166II GTNNAC 7 cut(s) 186, 496, 862, 1035, 1133, 1183, 1191
Hpy188I TCNGA 9 cut(s) 15, 30, 432, 604, 709, 891, 931, 1051, 1078
Hpy188III TCNNGA 2 cut(s) 722, 1109
Hpy8I GTNNAC 7 cut(s) 186, 496, 862, 1035, 1133, 1183, 1191
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 6 cut(s) 78, 295, 535, 553, 609, 1106
HpyCH4III ACNGT 2 cut(s) 354, 1018
HpyCH4IV ACGT 1 cut(s) 291
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 115
HpyF3I CTNAG 3 cut(s) 611, 1208, 1295
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 3 cut(s) 56, 846, 1164
Kzo9I GATC 4 cut(s) 633, 886, 1201, 1340
LmnI GCTCC 3 cut(s) 20, 597, 987
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 4 cut(s) 274, 436, 861, 882
MaeI CTAG 2 cut(s) 362, 414
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 638
MalI GATC 4 cut(s) 635, 888, 1203, 1342
MbiI CCGCTC 1 cut(s) 23
MboI GATC 4 cut(s) 633, 886, 1201, 1340
MhlI GDGCHC 3 cut(s) 602, 984, 1135
MluCI AATT 5 cut(s) 110, 1150, 1232, 1290, 1309
MlyI GAGTC 1 cut(s) 1064
MmeI TCCRAC 1 cut(s) 455
MroXI GAANNNNTTC 1 cut(s) 387
MseI TTAA 4 cut(s) 321, 879, 1173, 1229
MslI CAYNNNNRTG 1 cut(s) 720
MspI CCGG 3 cut(s) 331, 958, 1186
MspR9I CCNGG 4 cut(s) 331, 332, 507, 692
MvaI CCWGG 2 cut(s) 507, 692
MvnI CGCG 2 cut(s) 244, 1200
MwoI GCNNNNNNNGC 2 cut(s) 72, 115
NciI CCSGG 2 cut(s) 331, 332
NdeI CATATG 1 cut(s) 778
NdeII GATC 4 cut(s) 633, 886, 1201, 1340
NlaIII CATG 3 cut(s) 56, 846, 1164
NlaIV GGNNCC 3 cut(s) 327, 511, 807
NmuCI GTSAC 1 cut(s) 638
PceI AGGCCT 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 2 cut(s) 236, 305
PinAI ACCGGT 1 cut(s) 1185
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 1 cut(s) 1064
PpsI GAGTC 1 cut(s) 1064
PpuMI RGGWCCY 1 cut(s) 510
Psp124BI GAGCTC 1 cut(s) 602
Psp5II RGGWCCY 1 cut(s) 510
Psp6I CCWGG 2 cut(s) 505, 690
PspGI CCWGG 2 cut(s) 505, 690
PspN4I GGNNCC 3 cut(s) 327, 511, 807
PspPI GGNCC 3 cut(s) 41, 500, 510
PspPPI RGGWCCY 1 cut(s) 510
RsaI GTAC 4 cut(s) 58, 368, 1007, 1284
RsaNI GTAC 4 cut(s) 57, 367, 1006, 1283
RseI CAYNNNNRTG 1 cut(s) 720
SacI GAGCTC 1 cut(s) 602
SaqAI TTAA 4 cut(s) 321, 879, 1173, 1229
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 4 cut(s) 633, 886, 1201, 1340
Sau96I GGNCC 3 cut(s) 41, 500, 510
SchI GAGTC 1 cut(s) 1064
ScrFI CCNGG 4 cut(s) 331, 332, 507, 692
SduI GDGCHC 3 cut(s) 602, 984, 1135
SfaNI GCATC 4 cut(s) 274, 436, 861, 882
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 2 cut(s) 41, 510
SmaI CCCGGG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 720
SmlI CTYRAG 2 cut(s) 437, 514
SmoI CTYRAG 2 cut(s) 437, 514
Sse9I AATT 5 cut(s) 110, 1150, 1232, 1290, 1309
SseBI AGGCCT 1 cut(s) 619
SsiI CCGC 5 cut(s) 23, 244, 486, 790, 1198
SspMI CTAG 2 cut(s) 362, 414
SstI GAGCTC 1 cut(s) 602
StuI AGGCCT 1 cut(s) 619
StyD4I CCNGG 4 cut(s) 329, 330, 505, 690
TaaI ACNGT 2 cut(s) 354, 1018
TaiI ACGT 1 cut(s) 294
TaqI TCGA 1 cut(s) 903
TasI AATT 5 cut(s) 110, 1150, 1232, 1290, 1309
TatI WGTACW 2 cut(s) 366, 1005
TfiI GAWTC 2 cut(s) 236, 305
Tru1I TTAA 4 cut(s) 321, 879, 1173, 1229
Tru9I TTAA 4 cut(s) 321, 879, 1173, 1229
TscAI CASTG 4 cut(s) 111, 526, 643, 1023
TseFI GTSAC 1 cut(s) 638
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 638
TspGWI ACGGA 2 cut(s) 285, 1118
TspMI CCCGGG 1 cut(s) 330
TspRI CASTG 4 cut(s) 111, 526, 643, 1023
VneI GTGCAC 1 cut(s) 1131
VpaK11BI GGWCC 2 cut(s) 41, 510
XapI RAATTY 3 cut(s) 1150, 1290, 1309
XmaI CCCGGG 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 2 cut(s) 362, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.