Rw4G031980

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005812
Physical Location & Seq
Forward (+)
59143244 .. 59146150
2907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031980.1

Sequence Viewer

Length: 1305 bp
ATGGGAAGAGGTCTGATCGGAGCGGTTCTGATGTATCTTGGTCCAAGTGAGCATGGTACTCCTCGCTTTGTTCGCAGAGGAAGAAGGTTTAGTCGCATTTCTACGCTTCAATTCTCGCTCTGGGTTTTTGTCTTTGCAGCAATCTTTCTGCTTTATTTCATCTTCTTCGGCCTCACAATGTTGTCTACAGATGAGAATCATGAGATTGAAGAAAGTGCAGTTATTCCAGTAAAGGTAGAATCTTCGAGGACTGATGGTGGAAGCAAGCGTCGTAGGAAATCAACCCGGGAAGTTATGAACTTTGCACAGTTTTCACTAGAGTACATTGACCAAGAGGAAAAACTTCCTACGAGTGATATATATGAACCTAGATTTGGAGGGCATCCGACCCTTGAGGAAAGAGAGCAGTCCTTTTATGCCGTAAATCAAACTCTTCATTGCGGATTTGTCAACGGGCCTCCAGGGTCCTCAAGCACTGGGTTTGATTTAGATATGAAGGACAAGGCGTATATGAAGGATTGTAAGGTTGCAGTATCTTCTTGCATTTTTGGGAGCTCTGATTTTCTAAGAAGGCCTACAAGTAGAAAGATCAGTGACTATTCCAAGAAAAATGTTTGTTTTGTAATGTTTGTGGATGAAGAAAGCCTGGCAAAAATATCATCCGAGGGGCATTTTCCTGATGATACAGGACATATTGGCTTGTGGAGGATAGTTATAGTGAAGAACTTACCATATGCAGATATGCGGAGAACTGGAAAGGTGCCAAAACTTTTATCACATCGCCTTTTTCCCTCATCATGGTATTCCATTTGGGTTGACAGCAAGATGCGACTTAATACTGATCCGATGCAGATTATCGAATACTTTTTATGGCGAGCAAAATCAGAATATGCCATTTCCAATCACTATGACCGGCATTGTGTTTGGGAGGAGGTGCTCCAAAATAAGCGTCTAAATAAGTACAATCACACAGTGATTGATGAGCAGTTCACATTTTACCAATCTGATGGCCTCACCAAGTTTGACTCTTCAGACCCAAATACTCCTCTTCCAAGTTATGTTCCTGAAGGTTCATTTATTGTCCGTGCACATACACCTATTTCAAATTTATTTTCATGCCTCTGGTTTAATGAAGTTGACCGGTTTACCTCCCGCGATCAGCTAAGCTTTGCATATACTTTCTTAAAATTGAGAAGAATGAACCCAGATAGACCATTCTACCTAAATATGTTCAAGGACTGTGAGCGCAGAGCACTAGCAAAGCTATTCCGGCACAGGGCAGAGCTGTCTCCACCTGTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

434

Amino Acids

50.49

Weight (kDa)

8.9

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 106 - 425 1.9e-143 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 760
AccBSI CCGCTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 185
AccII CGCG 1 cut(s) 1155
AciI CCGC 4 cut(s) 23, 441, 745, 1153
AclWI GGATC 1 cut(s) 836
AcsI RAATTY 1 cut(s) 1105
AcuI CTGAAG 2 cut(s) 1014, 1086
AdeI CACNNNGTG 1 cut(s) 973
AfaI GTAC 3 cut(s) 58, 323, 962
AfiI CCNNNNNNNGG 3 cut(s) 374, 798, 1276
AgeI ACCGGT 1 cut(s) 1140
AgsI TTSAA 4 cut(s) 110, 209, 1104, 1234
AjnI CCWGG 2 cut(s) 460, 645
AjuI GAANNNNNNNTTGG 2 cut(s) 357, 389
AluBI AGCT 5 cut(s) 555, 1162, 1167, 1264, 1285
AluI AGCT 5 cut(s) 555, 1162, 1167, 1264, 1285
Alw21I GWGCWC 4 cut(s) 557, 939, 1090, 1255
Alw26I GTCTC 1 cut(s) 1293
Alw44I GTGCAC 1 cut(s) 1086
AlwI GGATC 1 cut(s) 836
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 4 cut(s) 169, 455, 572, 1009
ApaLI GTGCAC 1 cut(s) 1086
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 1 cut(s) 1105
ArsI GACNNNNNNTTYG 2 cut(s) 934, 966
AsiGI ACCGGT 1 cut(s) 1140
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 1 cut(s) 1248
AspS9I GGNCC 3 cut(s) 41, 455, 465
AsuC2I CCSGG 2 cut(s) 286, 287
AsuHPI GGTGA 1 cut(s) 1006
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 2 cut(s) 41, 465
BaeGI GKGCMC 1 cut(s) 1090
BanI GGYRCC 1 cut(s) 760
BanII GRGCYC 1 cut(s) 557
Bbv12I GWGCWC 4 cut(s) 557, 939, 1090, 1255
BbvI GCAGC 1 cut(s) 149
BccI CCATC 2 cut(s) 248, 1001
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 462, 647
BcnI CCSGG 2 cut(s) 286, 287
BcoDI GTCTC 1 cut(s) 1293
BfaI CTAG 3 cut(s) 317, 369, 1256
BfmI CTRYAG 1 cut(s) 186
BisI GCNGC 1 cut(s) 138
BlpI GCTNAGC 1 cut(s) 1163
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 4 cut(s) 286, 287, 462, 647
Bme18I GGWCC 2 cut(s) 41, 465
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 41, 455, 465
BmiI GGNNCC 2 cut(s) 466, 762
BmrFI CCNGG 4 cut(s) 286, 287, 462, 647
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 3 cut(s) 391, 816, 837
BmuI ACTGGG 1 cut(s) 486
BpmI CTGGAG 1 cut(s) 444
Bpu1102I GCTNAGC 1 cut(s) 1163
BpuEI CTTGAG 2 cut(s) 413, 454
BpuMI CCSGG 2 cut(s) 286, 287
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 3 cut(s) 285, 461, 663
BsaWI WCCGGW 1 cut(s) 1140
Bsc4I CCNNNNNNNGG 3 cut(s) 374, 798, 1276
Bse118I RCCGGY 2 cut(s) 912, 1140
Bse1I ACTGG 3 cut(s) 227, 481, 757
Bse3DI GCAATG 1 cut(s) 436
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 2 cut(s) 462, 647
BseDI CCNNGG 3 cut(s) 285, 461, 663
BseGI GGATG 3 cut(s) 382, 640, 659
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 3 cut(s) 374, 798, 1276
BseMI GCAATG 1 cut(s) 436
BseNI ACTGG 3 cut(s) 227, 481, 757
BseRI GAGGAG 3 cut(s) 51, 944, 1035
BseSI GKGCMC 1 cut(s) 1090
BseXI GCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 1155
BshFI GGCC 4 cut(s) 171, 457, 574, 1011
BshNI GGYRCC 1 cut(s) 760
BshTI ACCGGT 1 cut(s) 1140
BsiHKAI GWGCWC 4 cut(s) 557, 939, 1090, 1255
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 4 cut(s) 286, 913, 1141, 1270
BslI CCNNNNNNNGG 3 cut(s) 374, 798, 1276
BsmAI GTCTC 1 cut(s) 1293
BsnI GGCC 4 cut(s) 171, 457, 574, 1011
BsoBI CYCGRG 1 cut(s) 285
Bsp1286I GDGCHC 4 cut(s) 557, 939, 1090, 1255
Bsp143I GATC 4 cut(s) 15, 588, 841, 1156
Bsp1720I GCTNAGC 1 cut(s) 1163
BspACI CCGC 4 cut(s) 23, 441, 745, 1153
BspANI GGCC 4 cut(s) 171, 457, 574, 1011
BspFNI CGCG 1 cut(s) 1155
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 2 cut(s) 466, 762
BspPI GGATC 1 cut(s) 836
BspT107I GGYRCC 1 cut(s) 760
BsrBI CCGCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 436
BsrFI RCCGGY 2 cut(s) 912, 1140
BsrI ACTGG 3 cut(s) 227, 481, 757
BssAI RCCGGY 2 cut(s) 912, 1140
BssECI CCNNGG 3 cut(s) 285, 461, 663
BssMI GATC 4 cut(s) 15, 588, 841, 1156
Bst2UI CCWGG 2 cut(s) 462, 647
Bst4CI ACNGT 3 cut(s) 309, 973, 1241
Bst6I CTCTTC 3 cut(s) 438, 1033, 1053
BstC8I GCNNGC 2 cut(s) 266, 876
BstDEI CTNAG 2 cut(s) 566, 1163
BstF5I GGATG 3 cut(s) 382, 640, 659
BstFNI CGCG 1 cut(s) 1155
BstHHI GCGC 1 cut(s) 1248
BstKTI GATC 4 cut(s) 18, 591, 844, 1159
BstMAI GTCTC 1 cut(s) 1293
BstMBI GATC 4 cut(s) 15, 588, 841, 1156
BstMWI GCNNNNNNNGC 2 cut(s) 72, 1270
BstNI CCWGG 2 cut(s) 462, 647
BstSCI CCNGG 4 cut(s) 284, 285, 460, 645
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 1090
BstUI CGCG 1 cut(s) 1155
BstV1I GCAGC 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 1007
BsuRI GGCC 4 cut(s) 171, 457, 574, 1011
BtgZI GCGATG 1 cut(s) 764
BtsCI GGATG 3 cut(s) 382, 640, 659
BtsIMutI CAGTG 3 cut(s) 474, 598, 978
Cac8I GCNNGC 2 cut(s) 266, 876
CciI TCATGA 1 cut(s) 199
CfoI GCGC 1 cut(s) 1248
Cfr10I RCCGGY 2 cut(s) 912, 1140
Cfr13I GGNCC 3 cut(s) 41, 455, 465
Cfr9I CCCGGG 1 cut(s) 285
CseI GACGC 2 cut(s) 257, 938
Csp6I GTAC 3 cut(s) 57, 322, 961
CspAI ACCGGT 1 cut(s) 1140
CviAII CATG 4 cut(s) 53, 200, 798, 1116
CviQI GTAC 3 cut(s) 57, 322, 961
DdeI CTNAG 2 cut(s) 566, 1163
DpnI GATC 4 cut(s) 17, 590, 843, 1158
DpnII GATC 4 cut(s) 15, 588, 841, 1156
DraIII CACNNNGTG 1 cut(s) 973
Eam1104I CTCTTC 3 cut(s) 438, 1033, 1053
EarI CTCTTC 3 cut(s) 438, 1033, 1053
Ecl136II GAGCTC 1 cut(s) 555
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 557
Eco47I GGWCC 2 cut(s) 41, 465
Eco53kI GAGCTC 1 cut(s) 555
Eco57I CTGAAG 2 cut(s) 1014, 1086
Eco88I CYCGRG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 460, 645
EcoT38I GRGCYC 1 cut(s) 557
FaeI CATG 4 cut(s) 56, 203, 801, 1119
FatI CATG 4 cut(s) 52, 199, 797, 1115
FauI CCCGC 1 cut(s) 1160
FauNDI CATATG 1 cut(s) 733
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 3 cut(s) 369, 646, 647
FriOI GRGCYC 1 cut(s) 557
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 3 cut(s) 317, 369, 1256
GlaI GCGC 1 cut(s) 1247
GluI GCNGC 1 cut(s) 138
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 4 cut(s) 171, 457, 574, 1011
HapII CCGG 4 cut(s) 286, 913, 1141, 1270
HgaI GACGC 2 cut(s) 257, 938
HhaI GCGC 1 cut(s) 1248
Hin1II CATG 4 cut(s) 56, 203, 801, 1119
Hin6I GCGC 1 cut(s) 1246
HinP1I GCGC 1 cut(s) 1246
HincII GTYRAC 3 cut(s) 451, 817, 1138
HindII GTYRAC 3 cut(s) 451, 817, 1138
HindIII AAGCTT 1 cut(s) 1165
HinfI GANTC 3 cut(s) 196, 239, 1025
HpaII CCGG 4 cut(s) 286, 913, 1141, 1270
HphI GGTGA 1 cut(s) 1006
Hpy166II GTNNAC 7 cut(s) 186, 451, 817, 990, 1088, 1138, 1146
Hpy188III TCNNGA 3 cut(s) 200, 677, 1064
Hpy8I GTNNAC 7 cut(s) 186, 451, 817, 990, 1088, 1138, 1146
Hpy99I CGWCG 1 cut(s) 273
HpyAV CCTTC 5 cut(s) 78, 490, 508, 564, 1061
HpyCH4III ACNGT 3 cut(s) 309, 973, 1241
HpyCH4V TGCA 9 cut(s) 137, 218, 305, 530, 543, 737, 850, 1088, 1172
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 1270
HpyF3I CTNAG 2 cut(s) 566, 1163
Hsp92II CATG 4 cut(s) 56, 203, 801, 1119
HspAI GCGC 1 cut(s) 1246
Kzo9I GATC 4 cut(s) 15, 588, 841, 1156
LmnI GCTCC 3 cut(s) 20, 552, 942
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 3 cut(s) 391, 816, 837
MaeI CTAG 3 cut(s) 317, 369, 1256
MaeIII GTNAC 2 cut(s) 593, 1297
MalI GATC 4 cut(s) 17, 590, 843, 1158
MbiI CCGCTC 1 cut(s) 23
MboI GATC 4 cut(s) 15, 588, 841, 1156
MhlI GDGCHC 4 cut(s) 557, 939, 1090, 1255
MluCI AATT 3 cut(s) 110, 1105, 1187
MlyI GAGTC 1 cut(s) 1019
MmeI TCCRAC 1 cut(s) 410
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 3 cut(s) 834, 1128, 1184
MspI CCGG 4 cut(s) 286, 913, 1141, 1270
MspR9I CCNGG 4 cut(s) 286, 287, 462, 647
MvaI CCWGG 2 cut(s) 462, 647
MvnI CGCG 1 cut(s) 1155
MwoI GCNNNNNNNGC 2 cut(s) 72, 1270
NciI CCSGG 2 cut(s) 286, 287
NdeI CATATG 1 cut(s) 733
NdeII GATC 4 cut(s) 15, 588, 841, 1156
NlaIII CATG 4 cut(s) 56, 203, 801, 1119
NlaIV GGNNCC 2 cut(s) 466, 762
NmuCI GTSAC 1 cut(s) 593
PagI TCATGA 1 cut(s) 199
PceI AGGCCT 1 cut(s) 574
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 196, 239
PinAI ACCGGT 1 cut(s) 1140
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 1 cut(s) 1019
PpsI GAGTC 1 cut(s) 1019
PpuMI RGGWCCY 1 cut(s) 465
Psp124BI GAGCTC 1 cut(s) 557
Psp5II RGGWCCY 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 460, 645
PspGI CCWGG 2 cut(s) 460, 645
PspN4I GGNNCC 2 cut(s) 466, 762
PspPI GGNCC 3 cut(s) 41, 455, 465
PspPPI RGGWCCY 1 cut(s) 465
RsaI GTAC 3 cut(s) 58, 323, 962
RsaNI GTAC 3 cut(s) 57, 322, 961
SacI GAGCTC 1 cut(s) 557
SaqAI TTAA 3 cut(s) 834, 1128, 1184
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 4 cut(s) 15, 588, 841, 1156
Sau96I GGNCC 3 cut(s) 41, 455, 465
SchI GAGTC 1 cut(s) 1019
ScrFI CCNGG 4 cut(s) 286, 287, 462, 647
SduI GDGCHC 4 cut(s) 557, 939, 1090, 1255
SfaNI GCATC 3 cut(s) 391, 816, 837
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 2 cut(s) 41, 465
SmaI CCCGGG 1 cut(s) 287
SmlI CTYRAG 2 cut(s) 392, 469
SmoI CTYRAG 2 cut(s) 392, 469
Sse9I AATT 3 cut(s) 110, 1105, 1187
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 4 cut(s) 23, 441, 745, 1153
SspMI CTAG 3 cut(s) 317, 369, 1256
SstI GAGCTC 1 cut(s) 557
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 4 cut(s) 284, 285, 460, 645
TaaI ACNGT 3 cut(s) 309, 973, 1241
TaqI TCGA 2 cut(s) 245, 858
TasI AATT 3 cut(s) 110, 1105, 1187
TatI WGTACW 2 cut(s) 321, 960
TfiI GAWTC 2 cut(s) 196, 239
Tru1I TTAA 3 cut(s) 834, 1128, 1184
Tru9I TTAA 3 cut(s) 834, 1128, 1184
TscAI CASTG 3 cut(s) 481, 598, 978
TseFI GTSAC 1 cut(s) 593
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 593
TspGWI ACGGA 1 cut(s) 1073
TspMI CCCGGG 1 cut(s) 285
TspRI CASTG 3 cut(s) 481, 598, 978
VneI GTGCAC 1 cut(s) 1086
VpaK11BI GGWCC 2 cut(s) 41, 465
XapI RAATTY 1 cut(s) 1105
XmaI CCCGGG 1 cut(s) 285
XmiI GTMKAC 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 3 cut(s) 317, 369, 1256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.