MD16G1074300.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005812
Physical Location & Seq
Forward (+)
5196005 .. 5199273
3269 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1074300.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGAGGAGAGGTATAATCGGCGCGTTGTTGATGTATCAGGGTGAGCACGGCACTCGCCTTGTTCGCAAAGGAAGGAGGCTCGGTCGAATCGCCAGGAACAAATTCTGGTTCTGGGTTTTTCTCTTTGCAGCCATTTTTTTTATTCACTTGCTGCTCGTCGGCTTCAAAATGCATCCTCCTGCTAAACTAGAAGGTAGTGGTAATAGTATTAGTATTTCGATTCACCCAGAAATTTTGCCGTCTGGTGTTGGAAAACCACCCAGAAGCAAGCGTCGTGCCAAGTATTTGCCTTGTGAAGTTGGGTTGCTGGAATCAGTTGAGTATCTTGTTGAACCCTGGGAAGTCAGGAACTTCACACATTTTTCACTGGAATACATTGACCAGGAGGGATTACCTTCTCGTAGTAATATATACAAGCCTAGATTTGGAGGGCATCAGACACTTGAGGAAAGAGAACAATCCTTCTTTGCGAAAAATCAAACTCTGCATTGTGGATTTGTCAAGGGACCCCTGGGATTCTCAAGCTCTGGATTTGATTTAGACGTTAAGGACAAGGCATACATGAATGGCTGTAAGATTGTAGTATCTTCTTGCATTTTCGGAAGCTCTGACTTTCTAAGGAGGCCTACAAGCAAAAAGATGAGTGAATATTCCAAGAAAAATGTTTGTTTTGTAATGTTTGTGGATGAGCAAACATTGGCCAAACTATCAACAGAGGGACATGTTCCTGATGATACAGGACGAATTGGCTTGTGGAGGGTAGTCATAGTGAAGAATTTACCATATGAGGATATGCGCAGAACTGGAAAGGTGCCAAAACTTTTATCACATCGCCTTTTCCCCAATTCATGGTATTCAATTTGGCTTGACAGCAAGATGCGACTTAATACTGATCCGATGCTGATTATTGAATACTTTTTATGGCGCACAAAATCAGAGTATGCCATTTCCAACCATTATGGTCGCCATTGTGTCTGGGACGAGGTACTCCAAAACAAGCGTCTAGATAAGTACAATCACACAGCTATTGATGAACAATTTACATTTTACCAGTCTGATGGCCTCACCAAGTTTGACCCTTCAGACCCGAATACTCCTCTTCCAAGTTACGTTCCTGAAGGTTCATTTATAGTCCGGGCACATACACCAATTTCAAATTTGTTTTCCTGCCTTTGGTTTAATGAGGTTAACAGGTTTACCTCCCGTGATCAACTAAGCTTCGCATATACTTACCTAAAATTGAGAAGAATGAACCCAGAGAGACCATTCTACCTAAACATGTTCAAGGACTGTGAGCGTAGAGCATTAACGAAGCTATTCCAGCACAGGGAAGCACCGCCTTCTCCACCCATTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

453

Amino Acids

52.62

Weight (kDa)

9.52

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 123 - 442 1.6e-143 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 797
AccB1I GGYRCC 1 cut(s) 811
AccB7I CCANNNNNTGG 1 cut(s) 849
AccII CGCG 1 cut(s) 23
AciI CCGC 1 cut(s) 1337
AclWI GGATC 1 cut(s) 887
AcoI YGGCCR 1 cut(s) 699
AcsI RAATTY 4 cut(s) 101, 231, 775, 1156
AcuI CTGAAG 2 cut(s) 1065, 1137
AfaI GTAC 2 cut(s) 987, 1013
AfiI CCNNNNNNNGG 4 cut(s) 425, 849, 1173, 1327
AflIII ACRYGT 2 cut(s) 721, 1278
AgsI TTSAA 6 cut(s) 166, 332, 858, 911, 1155, 1285
AjnI CCWGG 4 cut(s) 92, 335, 381, 510
AluBI AGCT 5 cut(s) 525, 606, 1025, 1218, 1315
AluI AGCT 5 cut(s) 525, 606, 1025, 1218, 1315
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 1 cut(s) 1255
AlwI GGATC 1 cut(s) 887
AoxI GGCC 3 cut(s) 623, 699, 1060
ApeKI GCWGC 2 cut(s) 128, 151
ApoI RAATTY 4 cut(s) 101, 231, 775, 1156
ArsI GACNNNNNNTTYG 2 cut(s) 985, 1017
Asp700I GAANNNNTTC 2 cut(s) 101, 1316
AspLEI GCGC 3 cut(s) 23, 798, 927
AspS9I GGNCC 1 cut(s) 506
AsuC2I CCSGG 1 cut(s) 1136
AsuHPI GGTGA 3 cut(s) 53, 215, 1057
AvaII GGWCC 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 1141
BalI TGGCCA 1 cut(s) 701
BanI GGYRCC 1 cut(s) 811
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 2 cut(s) 138, 140
BccI CCATC 1 cut(s) 1052
BceAI ACGGC 2 cut(s) 64, 223
BcgI CGANNNNNNTGC 2 cut(s) 35, 69
BciT130I CCWGG 4 cut(s) 94, 337, 383, 512
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 1 cut(s) 1136
BcoDI GTCTC 1 cut(s) 1255
BfaI CTAG 3 cut(s) 188, 420, 1004
BisI GCNGC 2 cut(s) 129, 152
BlsI GCNGC 2 cut(s) 130, 153
Bme1390I CCNGG 5 cut(s) 94, 337, 383, 512, 1136
Bme18I GGWCC 1 cut(s) 506
BmgT120I GGNCC 1 cut(s) 506
BmiI GGNNCC 3 cut(s) 507, 508, 813
BmrFI CCNGG 5 cut(s) 94, 337, 383, 512, 1136
BmsI GCATC 4 cut(s) 181, 442, 867, 888
BpuEI CTTGAG 2 cut(s) 464, 505
BpuMI CCSGG 1 cut(s) 1136
BsaI GGTCTC 1 cut(s) 1255
BsaJI CCNNGG 4 cut(s) 335, 336, 510, 511
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 849, 1173, 1327
Bse1I ACTGG 3 cut(s) 372, 808, 1051
BseBI CCWGG 4 cut(s) 94, 337, 383, 512
BseDI CCNNGG 4 cut(s) 335, 336, 510, 511
BseGI GGATG 2 cut(s) 172, 691
BseLI CCNNNNNNNGG 4 cut(s) 425, 849, 1173, 1327
BseNI ACTGG 3 cut(s) 372, 808, 1051
BseRI GAGGAG 2 cut(s) 19, 1086
BseSI GKGCMC 1 cut(s) 1141
BseXI GCAGC 2 cut(s) 138, 140
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 3 cut(s) 625, 701, 1062
BshNI GGYRCC 1 cut(s) 811
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 1 cut(s) 1135
BslFI GGGAC 3 cut(s) 519, 732, 992
BslI CCNNNNNNNGG 4 cut(s) 425, 849, 1173, 1327
BsmAI GTCTC 1 cut(s) 1255
BsmFI GGGAC 3 cut(s) 519, 732, 992
BsnI GGCC 3 cut(s) 625, 701, 1062
Bso31I GGTCTC 1 cut(s) 1255
Bsp1286I GDGCHC 2 cut(s) 48, 1141
Bsp143I GATC 2 cut(s) 892, 1207
BspACI CCGC 1 cut(s) 1337
BspANI GGCC 3 cut(s) 625, 701, 1062
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 507, 508, 813
BspPI GGATC 1 cut(s) 887
BspT107I GGYRCC 1 cut(s) 811
BspTNI GGTCTC 1 cut(s) 1255
BsrI ACTGG 3 cut(s) 372, 808, 1051
BssECI CCNNGG 4 cut(s) 335, 336, 510, 511
BssMI GATC 2 cut(s) 892, 1207
Bst2UI CCWGG 4 cut(s) 94, 337, 383, 512
Bst4CI ACNGT 1 cut(s) 1292
Bst6I CTCTTC 1 cut(s) 1104
BstC8I GCNNGC 1 cut(s) 269
BstDEI CTNAG 2 cut(s) 617, 1214
BstF5I GGATG 2 cut(s) 172, 691
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 3 cut(s) 23, 798, 927
BstKTI GATC 2 cut(s) 895, 1210
BstMAI GTCTC 1 cut(s) 1255
BstMBI GATC 2 cut(s) 892, 1207
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 2 cut(s) 63, 1321
BstNI CCWGG 4 cut(s) 94, 337, 383, 512
BstNSI RCATGY 2 cut(s) 725, 1282
BstSCI CCNGG 5 cut(s) 92, 335, 381, 510, 1134
BstSLI GKGCMC 1 cut(s) 1141
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 2 cut(s) 138, 140
BstXI CCANNNNNNTGG 1 cut(s) 1058
BsuRI GGCC 3 cut(s) 625, 701, 1062
BtgZI GCGATG 1 cut(s) 815
BtsCI GGATG 2 cut(s) 172, 691
BtsIMutI CAGTG 1 cut(s) 365
Cac8I GCNNGC 1 cut(s) 269
CfoI GCGC 3 cut(s) 23, 798, 927
Cfr13I GGNCC 1 cut(s) 506
CseI GACGC 2 cut(s) 260, 989
Csp6I GTAC 2 cut(s) 986, 1012
CviAII CATG 4 cut(s) 562, 722, 849, 1279
CviQI GTAC 2 cut(s) 986, 1012
DdeI CTNAG 2 cut(s) 617, 1214
DpnI GATC 2 cut(s) 894, 1209
DpnII GATC 2 cut(s) 892, 1207
EaeI YGGCCR 1 cut(s) 699
Eam1104I CTCTTC 1 cut(s) 1104
EarI CTCTTC 1 cut(s) 1104
Eco147I AGGCCT 1 cut(s) 625
Eco31I GGTCTC 1 cut(s) 1255
Eco47I GGWCC 1 cut(s) 506
Eco57I CTGAAG 2 cut(s) 1065, 1137
EcoO109I RGGNCCY 1 cut(s) 506
EcoRII CCWGG 4 cut(s) 92, 335, 381, 510
EcoT22I ATGCAT 1 cut(s) 174
FaeI CATG 4 cut(s) 565, 725, 852, 1282
FaqI GGGAC 3 cut(s) 519, 732, 992
FatI CATG 4 cut(s) 561, 721, 848, 1278
FauNDI CATATG 1 cut(s) 784
FbaI TGATCA 1 cut(s) 1207
Fnu4HI GCNGC 2 cut(s) 129, 152
FokI GGATG 2 cut(s) 159, 698
Fsp4HI GCNGC 2 cut(s) 129, 152
FspBI CTAG 3 cut(s) 188, 420, 1004
FspI TGCGCA 1 cut(s) 797
GlaI GCGC 3 cut(s) 22, 797, 926
GluI GCNGC 2 cut(s) 129, 152
HaeIII GGCC 3 cut(s) 625, 701, 1062
HapII CCGG 1 cut(s) 1135
HgaI GACGC 2 cut(s) 260, 989
HhaI GCGC 3 cut(s) 23, 798, 927
Hin1II CATG 4 cut(s) 565, 725, 852, 1282
Hin6I GCGC 3 cut(s) 21, 796, 925
HinP1I GCGC 3 cut(s) 21, 796, 925
HincII GTYRAC 1 cut(s) 1189
HindII GTYRAC 1 cut(s) 1189
HindIII AAGCTT 1 cut(s) 1216
HinfI GANTC 4 cut(s) 87, 220, 311, 516
HpaI GTTAAC 1 cut(s) 1189
HpaII CCGG 1 cut(s) 1135
HphI GGTGA 3 cut(s) 53, 215, 1057
Hpy166II GTNNAC 2 cut(s) 1189, 1197
Hpy188I TCNGA 7 cut(s) 438, 602, 610, 897, 937, 1057, 1084
Hpy188III TCNNGA 5 cut(s) 346, 528, 728, 1004, 1115
Hpy8I GTNNAC 2 cut(s) 1189, 1197
Hpy99I CGWCG 2 cut(s) 161, 276
HpyAV CCTTC 7 cut(s) 66, 185, 405, 472, 1089, 1112, 1350
HpyCH4III ACNGT 1 cut(s) 1292
HpyCH4IV ACGT 2 cut(s) 543, 1110
HpyCH4V TGCA 4 cut(s) 128, 172, 487, 594
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 1321
HpyF3I CTNAG 2 cut(s) 617, 1214
HpySE526I ACGT 2 cut(s) 543, 1110
Hsp92II CATG 4 cut(s) 565, 725, 852, 1282
HspAI GCGC 3 cut(s) 21, 796, 925
KflI GGGWCCC 1 cut(s) 506
Ksp22I TGATCA 1 cut(s) 1207
KspAI GTTAAC 1 cut(s) 1189
Kzo9I GATC 2 cut(s) 892, 1207
Lsp1109I GCAGC 2 cut(s) 138, 140
LweI GCATC 4 cut(s) 181, 442, 867, 888
MaeI CTAG 3 cut(s) 188, 420, 1004
MaeII ACGT 2 cut(s) 543, 1110
MaeIII GTNAC 1 cut(s) 1106
MalI GATC 2 cut(s) 894, 1209
MboI GATC 2 cut(s) 892, 1207
MboII GAAGA 4 cut(s) 579, 784, 1091, 1257
MhlI GDGCHC 2 cut(s) 48, 1141
MlsI TGGCCA 1 cut(s) 701
MluNI TGGCCA 1 cut(s) 701
MmeI TCCRAC 2 cut(s) 229, 975
Mox20I TGGCCA 1 cut(s) 701
Mph1103I ATGCAT 1 cut(s) 174
MroXI GAANNNNTTC 2 cut(s) 101, 1316
MscI TGGCCA 1 cut(s) 701
MseI TTAA 5 cut(s) 546, 885, 1179, 1188, 1307
Msp20I TGGCCA 1 cut(s) 701
MspI CCGG 1 cut(s) 1135
MspR9I CCNGG 5 cut(s) 94, 337, 383, 512, 1136
MvaI CCWGG 4 cut(s) 94, 337, 383, 512
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 63, 1321
NciI CCSGG 1 cut(s) 1136
NdeI CATATG 1 cut(s) 784
NdeII GATC 2 cut(s) 892, 1207
NlaIII CATG 4 cut(s) 565, 725, 852, 1282
NlaIV GGNNCC 3 cut(s) 507, 508, 813
NsbI TGCGCA 1 cut(s) 797
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 2 cut(s) 725, 1282
PasI CCCWGGG 2 cut(s) 336, 511
PceI AGGCCT 1 cut(s) 625
PciI ACATGT 2 cut(s) 721, 1278
PdmI GAANNNNTTC 2 cut(s) 101, 1316
PfeI GAWTC 4 cut(s) 87, 220, 311, 516
PflMI CCANNNNNTGG 1 cut(s) 849
PkrI GCNGC 2 cut(s) 130, 153
PpuMI RGGWCCY 1 cut(s) 506
PscI ACATGT 2 cut(s) 721, 1278
Psp5II RGGWCCY 1 cut(s) 506
Psp6I CCWGG 4 cut(s) 92, 335, 381, 510
PspGI CCWGG 4 cut(s) 92, 335, 381, 510
PspN4I GGNNCC 3 cut(s) 507, 508, 813
PspPI GGNCC 1 cut(s) 506
PspPPI RGGWCCY 1 cut(s) 506
RsaI GTAC 2 cut(s) 987, 1013
RsaNI GTAC 2 cut(s) 986, 1012
SaqAI TTAA 5 cut(s) 546, 885, 1179, 1188, 1307
SatI GCNGC 2 cut(s) 129, 152
Sau3AI GATC 2 cut(s) 892, 1207
Sau96I GGNCC 1 cut(s) 506
ScrFI CCNGG 5 cut(s) 94, 337, 383, 512, 1136
SduI GDGCHC 2 cut(s) 48, 1141
SfaNI GCATC 4 cut(s) 181, 442, 867, 888
SinI GGWCC 1 cut(s) 506
SmlI CTYRAG 2 cut(s) 443, 520
SmoI CTYRAG 2 cut(s) 443, 520
SseBI AGGCCT 1 cut(s) 625
SsiI CCGC 1 cut(s) 1337
SspI AATATT 1 cut(s) 650
SspMI CTAG 3 cut(s) 188, 420, 1004
StuI AGGCCT 1 cut(s) 625
StyD4I CCNGG 5 cut(s) 92, 335, 381, 510, 1134
TaaI ACNGT 1 cut(s) 1292
TaiI ACGT 2 cut(s) 546, 1113
TaqI TCGA 2 cut(s) 85, 218
TaqII GACCGA 1 cut(s) 71
TatI WGTACW 1 cut(s) 1011
TfiI GAWTC 4 cut(s) 87, 220, 311, 516
Tru1I TTAA 5 cut(s) 546, 885, 1179, 1188, 1307
Tru9I TTAA 5 cut(s) 546, 885, 1179, 1188, 1307
TscAI CASTG 1 cut(s) 372
TseI GCWGC 2 cut(s) 128, 151
TspDTI ATGAA 5 cut(s) 578, 837, 1047, 1113, 1265
TspRI CASTG 1 cut(s) 372
Van91I CCANNNNNTGG 1 cut(s) 849
VpaK11BI GGWCC 1 cut(s) 506
XapI RAATTY 4 cut(s) 101, 231, 775, 1156
XbaI TCTAGA 1 cut(s) 1003
XceI RCATGY 2 cut(s) 725, 1282
XmnI GAANNNNTTC 2 cut(s) 101, 1316
XspI CTAG 3 cut(s) 188, 420, 1004
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.