Rh4BG386200

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005812
Physical Location & Seq
Forward (+)
54024880 .. 54027932
3053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG386200.1

Sequence Viewer

Length: 1371 bp
ATGAGAAGAGTTCTGATCGGAGCGGTTCTGATGTATCTTGGTCCAAGTGAGCATGGTACTCCTCGCTTTGTTCGCAGAGGAAGAAGGTTTAGTCGCATTTCTACACTTCAATTCTCGCTCTGGGTTTTTGTCTTTGCAGCAATCTTTCTGCTTTATTTCATCTTCTTCGGCCTCACAATGTTGTCTACAGATGAGAATCATGAGATTGAAGAAAGTGCAGATATTTCAGTAAAGGATGATATTTCAGTAAAGAATGAATCTTCGAGGACTAATGGTGGAAGCAAGCATCGTAGGAAATATACTCCTTGCGACGTTCCGTTAAAGGAATCAGTTTATTATTTTAAGGAACCCCGGGAAGTTATGAACTTTACACAGTTTTCACTAGAGTACATTGACCAAGAGGAAAAACTTCCTACAAGTGATATATATGAACCTAGATTTGGAGGGCATCCGACCCTTGAGGAAAGAGAGCAGTCCTTTTATGCCGTAAATCAAACTCTTCATTGCGGATTTGTCAACGGGCCTCCAGGGTACTCAAGCACTGGATTTGATTTAGATATGAAGGACAAGGCGTATATGAAGGATTGTAAGGTTGCAGTATCTTCTTGCATTTTTGGGAGCTCTGATTTTCTAAGAAGGCCTACAAGTAGAAAGATCAGTGACTATTCCAAGAAAAATGTTTGTTTTGTAATGTTTGTGGATGAAGAAAGCCTGGCAAAAATATCATCCGAGGGGCATTTTCCTGATGATACAGGACATATTGGCTTGTGGAGGATAGTTATAGTGAAGAACTTACCATATGCAGATATGCGGAGAACTGGAAAGGTGCCAAAACTTTTATCACATCGCCTTTTTCCCTCATCATGGTATTCCATTTGGGTTGACAGCAAGATGCGACTTAATACTGATCCGATGCAGATTATCGAATACTTTTTATGGCGAGCAAAATCAGAATATGCCATTTCCAATCACTATGACCGGCATTGTGTTTGGGAGGAGGTGCTCCAAAATAAGCGTCTAAATAAGTACAATCACACAGTGATTGATGAGCAGTTCACATTTTACCAATCTGATGGCCTCACCAAGTTTGACTCTTCAGACCCAAATACTCCTCTTCCAAGTTATGTTCCCGAAGGTTCATTTATTGTCCGTGCACATACACCTATTTCAAATTTATTTTCATGCCTCTGGTTTAATGAAGTTGACCGGTTTACCTCCCGCGATCAGCTAAGCTTTGCATATACTTTCTTAAAATTGAGAAGAATGAACCCAGATAGACCATTCTATCTAAATATGTTCAAGGACTGTGAGCGCAGAGCACTAGCAAAGCTATTCCGGCACAGGGCAGAGCTGTCTCCACCTGTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

456

Amino Acids

53.37

Weight (kDa)

8.6

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 128 - 447 3e-143 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 826
AccBSI CCGCTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 185
AccII CGCG 1 cut(s) 1221
AciI CCGC 4 cut(s) 23, 507, 811, 1219
AclWI GGATC 1 cut(s) 902
AcsI RAATTY 1 cut(s) 1171
AcuI CTGAAG 1 cut(s) 1080
AdeI CACNNNGTG 1 cut(s) 1039
AfaI GTAC 4 cut(s) 58, 389, 533, 1028
AfiI CCNNNNNNNGG 3 cut(s) 440, 864, 1342
AgeI ACCGGT 1 cut(s) 1206
AgsI TTSAA 4 cut(s) 110, 209, 1170, 1300
AjnI CCWGG 2 cut(s) 526, 711
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 5 cut(s) 621, 1228, 1233, 1330, 1351
AluI AGCT 5 cut(s) 621, 1228, 1233, 1330, 1351
Alw21I GWGCWC 4 cut(s) 623, 1005, 1156, 1321
Alw26I GTCTC 1 cut(s) 1359
Alw44I GTGCAC 1 cut(s) 1152
AlwI GGATC 1 cut(s) 902
Ama87I CYCGRG 1 cut(s) 351
AoxI GGCC 4 cut(s) 169, 521, 638, 1075
ApaLI GTGCAC 1 cut(s) 1152
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 1 cut(s) 1171
ArsI GACNNNNNNTTYG 2 cut(s) 1000, 1032
AsiGI ACCGGT 1 cut(s) 1206
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 1 cut(s) 1314
AspS9I GGNCC 2 cut(s) 41, 521
AsuC2I CCSGG 2 cut(s) 352, 353
AsuHPI GGTGA 1 cut(s) 1072
AvaI CYCGRG 1 cut(s) 351
AvaII GGWCC 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 1156
BanI GGYRCC 1 cut(s) 826
BanII GRGCYC 1 cut(s) 623
Bbv12I GWGCWC 4 cut(s) 623, 1005, 1156, 1321
BbvI GCAGC 1 cut(s) 149
BccI CCATC 1 cut(s) 1067
BceAI ACGGC 1 cut(s) 470
BciT130I CCWGG 2 cut(s) 528, 713
BcnI CCSGG 2 cut(s) 352, 353
BcoDI GTCTC 1 cut(s) 1359
BfaI CTAG 3 cut(s) 383, 435, 1322
BfmI CTRYAG 1 cut(s) 186
BisI GCNGC 1 cut(s) 138
BlpI GCTNAGC 1 cut(s) 1229
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 4 cut(s) 352, 353, 528, 713
Bme18I GGWCC 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 351
BmgT120I GGNCC 2 cut(s) 41, 521
BmiI GGNNCC 2 cut(s) 348, 828
BmrFI CCNGG 4 cut(s) 352, 353, 528, 713
BmsI GCATC 4 cut(s) 295, 457, 882, 903
BpmI CTGGAG 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 1229
BpuEI CTTGAG 2 cut(s) 479, 520
BpuMI CCSGG 2 cut(s) 352, 353
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 350, 351, 527, 729
BsaWI WCCGGW 1 cut(s) 1206
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 864, 1342
Bse118I RCCGGY 2 cut(s) 978, 1206
Bse1I ACTGG 2 cut(s) 547, 823
Bse3DI GCAATG 1 cut(s) 502
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 2 cut(s) 528, 713
BseDI CCNNGG 4 cut(s) 350, 351, 527, 729
BseGI GGATG 4 cut(s) 241, 448, 706, 725
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 3 cut(s) 440, 864, 1342
BseMI GCAATG 1 cut(s) 502
BseNI ACTGG 2 cut(s) 547, 823
BseRI GAGGAG 3 cut(s) 51, 1010, 1101
BseSI GKGCMC 1 cut(s) 1156
BseXI GCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 1221
BshFI GGCC 4 cut(s) 171, 523, 640, 1077
BshNI GGYRCC 1 cut(s) 826
BshTI ACCGGT 1 cut(s) 1206
BsiHKAI GWGCWC 4 cut(s) 623, 1005, 1156, 1321
BsiHKCI CYCGRG 1 cut(s) 351
BsiSI CCGG 4 cut(s) 352, 979, 1207, 1336
BslI CCNNNNNNNGG 3 cut(s) 440, 864, 1342
BsmAI GTCTC 1 cut(s) 1359
BsnI GGCC 4 cut(s) 171, 523, 640, 1077
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 4 cut(s) 623, 1005, 1156, 1321
Bsp143I GATC 4 cut(s) 15, 654, 907, 1222
Bsp1720I GCTNAGC 1 cut(s) 1229
BspACI CCGC 4 cut(s) 23, 507, 811, 1219
BspANI GGCC 4 cut(s) 171, 523, 640, 1077
BspFNI CGCG 1 cut(s) 1221
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 2 cut(s) 348, 828
BspPI GGATC 1 cut(s) 902
BspT107I GGYRCC 1 cut(s) 826
BsrBI CCGCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 502
BsrFI RCCGGY 2 cut(s) 978, 1206
BsrI ACTGG 2 cut(s) 547, 823
BssAI RCCGGY 2 cut(s) 978, 1206
BssECI CCNNGG 4 cut(s) 350, 351, 527, 729
BssMI GATC 4 cut(s) 15, 654, 907, 1222
Bst2UI CCWGG 2 cut(s) 528, 713
Bst4CI ACNGT 3 cut(s) 375, 1039, 1307
Bst6I CTCTTC 3 cut(s) 504, 1099, 1119
BstC8I GCNNGC 2 cut(s) 284, 942
BstDEI CTNAG 2 cut(s) 632, 1229
BstF5I GGATG 4 cut(s) 241, 448, 706, 725
BstFNI CGCG 1 cut(s) 1221
BstHHI GCGC 1 cut(s) 1314
BstKTI GATC 4 cut(s) 18, 657, 910, 1225
BstMAI GTCTC 1 cut(s) 1359
BstMBI GATC 4 cut(s) 15, 654, 907, 1222
BstMWI GCNNNNNNNGC 2 cut(s) 72, 1336
BstNI CCWGG 2 cut(s) 528, 713
BstSCI CCNGG 4 cut(s) 350, 351, 526, 711
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 1156
BstUI CGCG 1 cut(s) 1221
BstV1I GCAGC 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 1073
BsuRI GGCC 4 cut(s) 171, 523, 640, 1077
BtgZI GCGATG 1 cut(s) 830
BtsCI GGATG 4 cut(s) 241, 448, 706, 725
BtsIMutI CAGTG 3 cut(s) 540, 664, 1044
Cac8I GCNNGC 2 cut(s) 284, 942
CciI TCATGA 1 cut(s) 199
CfoI GCGC 1 cut(s) 1314
Cfr10I RCCGGY 2 cut(s) 978, 1206
Cfr13I GGNCC 2 cut(s) 41, 521
Cfr9I CCCGGG 1 cut(s) 351
CseI GACGC 1 cut(s) 1004
Csp6I GTAC 4 cut(s) 57, 388, 532, 1027
CspAI ACCGGT 1 cut(s) 1206
CviAII CATG 4 cut(s) 53, 200, 864, 1182
CviQI GTAC 4 cut(s) 57, 388, 532, 1027
DdeI CTNAG 2 cut(s) 632, 1229
DpnI GATC 4 cut(s) 17, 656, 909, 1224
DpnII GATC 4 cut(s) 15, 654, 907, 1222
DraIII CACNNNGTG 1 cut(s) 1039
Eam1104I CTCTTC 3 cut(s) 504, 1099, 1119
EarI CTCTTC 3 cut(s) 504, 1099, 1119
Ecl136II GAGCTC 1 cut(s) 621
Eco147I AGGCCT 1 cut(s) 640
Eco24I GRGCYC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 621
Eco57I CTGAAG 1 cut(s) 1080
Eco88I CYCGRG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 621
EcoRII CCWGG 2 cut(s) 526, 711
EcoT38I GRGCYC 1 cut(s) 623
FaeI CATG 4 cut(s) 56, 203, 867, 1185
FatI CATG 4 cut(s) 52, 199, 863, 1181
FauI CCCGC 1 cut(s) 1226
FauNDI CATATG 1 cut(s) 799
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 4 cut(s) 248, 435, 712, 713
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 3 cut(s) 383, 435, 1322
GlaI GCGC 1 cut(s) 1313
GluI GCNGC 1 cut(s) 138
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 4 cut(s) 171, 523, 640, 1077
HapII CCGG 4 cut(s) 352, 979, 1207, 1336
HgaI GACGC 1 cut(s) 1004
HhaI GCGC 1 cut(s) 1314
Hin1II CATG 4 cut(s) 56, 203, 867, 1185
Hin6I GCGC 1 cut(s) 1312
HinP1I GCGC 1 cut(s) 1312
HincII GTYRAC 3 cut(s) 517, 883, 1204
HindII GTYRAC 3 cut(s) 517, 883, 1204
HindIII AAGCTT 1 cut(s) 1231
HinfI GANTC 4 cut(s) 196, 257, 326, 1091
HpaII CCGG 4 cut(s) 352, 979, 1207, 1336
HphI GGTGA 1 cut(s) 1072
Hpy166II GTNNAC 7 cut(s) 186, 517, 883, 1056, 1154, 1204, 1212
Hpy188III TCNNGA 3 cut(s) 200, 743, 1130
Hpy8I GTNNAC 7 cut(s) 186, 517, 883, 1056, 1154, 1204, 1212
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 5 cut(s) 78, 556, 574, 630, 1127
HpyCH4III ACNGT 3 cut(s) 375, 1039, 1307
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 8 cut(s) 137, 218, 596, 609, 803, 916, 1154, 1238
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 1336
HpyF3I CTNAG 2 cut(s) 632, 1229
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 4 cut(s) 56, 203, 867, 1185
HspAI GCGC 1 cut(s) 1312
Kzo9I GATC 4 cut(s) 15, 654, 907, 1222
LmnI GCTCC 3 cut(s) 20, 618, 1008
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 4 cut(s) 295, 457, 882, 903
MaeI CTAG 3 cut(s) 383, 435, 1322
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 659, 1363
MalI GATC 4 cut(s) 17, 656, 909, 1224
MbiI CCGCTC 1 cut(s) 23
MboI GATC 4 cut(s) 15, 654, 907, 1222
MhlI GDGCHC 4 cut(s) 623, 1005, 1156, 1321
MluCI AATT 3 cut(s) 110, 1171, 1253
MlyI GAGTC 1 cut(s) 1085
MmeI TCCRAC 1 cut(s) 476
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 5 cut(s) 320, 342, 900, 1194, 1250
MspI CCGG 4 cut(s) 352, 979, 1207, 1336
MspR9I CCNGG 4 cut(s) 352, 353, 528, 713
MvaI CCWGG 2 cut(s) 528, 713
MvnI CGCG 1 cut(s) 1221
MwoI GCNNNNNNNGC 2 cut(s) 72, 1336
NciI CCSGG 2 cut(s) 352, 353
NdeI CATATG 1 cut(s) 799
NdeII GATC 4 cut(s) 15, 654, 907, 1222
NlaIII CATG 4 cut(s) 56, 203, 867, 1185
NlaIV GGNNCC 2 cut(s) 348, 828
NmuCI GTSAC 1 cut(s) 659
PagI TCATGA 1 cut(s) 199
PceI AGGCCT 1 cut(s) 640
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 3 cut(s) 196, 257, 326
PinAI ACCGGT 1 cut(s) 1206
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 1 cut(s) 1085
PpsI GAGTC 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 623
Psp6I CCWGG 2 cut(s) 526, 711
PspGI CCWGG 2 cut(s) 526, 711
PspN4I GGNNCC 2 cut(s) 348, 828
PspPI GGNCC 2 cut(s) 41, 521
RsaI GTAC 4 cut(s) 58, 389, 533, 1028
RsaNI GTAC 4 cut(s) 57, 388, 532, 1027
SacI GAGCTC 1 cut(s) 623
SaqAI TTAA 5 cut(s) 320, 342, 900, 1194, 1250
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 4 cut(s) 15, 654, 907, 1222
Sau96I GGNCC 2 cut(s) 41, 521
SchI GAGTC 1 cut(s) 1085
ScrFI CCNGG 4 cut(s) 352, 353, 528, 713
SduI GDGCHC 4 cut(s) 623, 1005, 1156, 1321
SfaNI GCATC 4 cut(s) 295, 457, 882, 903
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 1 cut(s) 41
SmaI CCCGGG 1 cut(s) 353
SmlI CTYRAG 2 cut(s) 458, 535
SmoI CTYRAG 2 cut(s) 458, 535
Sse9I AATT 3 cut(s) 110, 1171, 1253
SseBI AGGCCT 1 cut(s) 640
SsiI CCGC 4 cut(s) 23, 507, 811, 1219
SspMI CTAG 3 cut(s) 383, 435, 1322
SstI GAGCTC 1 cut(s) 623
StuI AGGCCT 1 cut(s) 640
StyD4I CCNGG 4 cut(s) 350, 351, 526, 711
TaaI ACNGT 3 cut(s) 375, 1039, 1307
TaiI ACGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 263, 924
TasI AATT 3 cut(s) 110, 1171, 1253
TatI WGTACW 2 cut(s) 387, 1026
TfiI GAWTC 3 cut(s) 196, 257, 326
Tru1I TTAA 5 cut(s) 320, 342, 900, 1194, 1250
Tru9I TTAA 5 cut(s) 320, 342, 900, 1194, 1250
TscAI CASTG 3 cut(s) 547, 664, 1044
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 659
TspGWI ACGGA 2 cut(s) 306, 1139
TspMI CCCGGG 1 cut(s) 351
TspRI CASTG 3 cut(s) 547, 664, 1044
VneI GTGCAC 1 cut(s) 1152
VpaK11BI GGWCC 1 cut(s) 41
XapI RAATTY 1 cut(s) 1171
XmaI CCCGGG 1 cut(s) 351
XmiI GTMKAC 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 3 cut(s) 383, 435, 1322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.