Rh4CG400700

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005812
Physical Location & Seq
Forward (+)
64926100 .. 64930079
3980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG400700.1

Sequence Viewer

Length: 1137 bp
ATGAGAAGAGTTCTGATCGGAGCGGTTCTGATGTATCTTGGTCCAAGTGAGCATGGTACTCCTCGCTTTGTTCGCAGAGGAAGAAGGTTTAGTCGCATTTCTACACTTCAATTCTCGCTCTGGGTTTTTGTCTTTGCAGCAATCTTTCTGCTTTATTTCATCTTCTTCGGCCTCACAATGTTGTCTACAGATGAGAATCATGAGATTGAAGAAAGTGCAGATATTTCAGTAAAGGATGATATTTCAGTAAAGAATGAATCTTCGAGGACTAATGGTGGAAGCAAGCATCGTAGGAAATATACTCCTTGCGACGTTCCGTTAAAGGAATCAGTTTATTATTTTAAGGAACCCCGGGAAGTTATGAACTTTACACAGTTTTCACTAGAGTACATTGACCAAGAGGAAAAACTTCCTACAAGTGATATATATGAACCTAGATTTGGAGGGCATCCGACCCTTGAGGAAAGAGAGCAGTCCTTTTATGCCGTAAATCAAACTCTTCATTGCGGATTTGTCAACGGGCCTCCAGGGTACTCAAGCACTGGATTTGATTTAGATATGAAGGACAAGGCGTATATGAAGGATTGTAAGGTTGCAGTATCTTCTTGCATTTTTGGGAGCTCTGATTTTCTAAGAAGGCCTACAAGTAGAAAGATCAGTGACTATTCCAAGAAAAATGTTTGTTTTGTAATGTTTGTGGATGAAGAAAGCCTGGCAAAAATATCATCCGAGGGGCATTTTCCTGATGATACAGGACATATTGGCTTGTGGAGGATAGTTATAGTGAAGAACTTACCATATGCAGATATGCGGAGAACTGGAAAGGTGCCAAAACTTTTATCACATCGCCTTTTTCCCTCATCATGGTATTCCATTTGGGTTGACAGCAAGATGCGACTTAATACTGATCCGATGCAGATTATCGAATACTTTTTATGGCGAGCAAAATCAGAATATGCCATTTCCAATCACTATGACCGGCATTGTGTTTGGGAGGAGGTGCTCCAAAATAAGCGTCTAAATAAGTACAATCACACAGTGATTGATGAGCAGTTCACATTTTACCAATCTGATGGCCTCACCAAGTTTGACTCTTCAGACCCAAATACTCCTCTTCCAAGTTGTGAGTGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

378

Amino Acids

44.05

Weight (kDa)

6.84

Isoelectric Point (pI)

54.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 128 - 368 8.5e-104 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 826
AccBSI CCGCTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 185
AciI CCGC 3 cut(s) 23, 507, 811
AclWI GGATC 1 cut(s) 902
AcuI CTGAAG 1 cut(s) 1080
AdeI CACNNNGTG 1 cut(s) 1039
AfaI GTAC 4 cut(s) 58, 389, 533, 1028
AfiI CCNNNNNNNGG 2 cut(s) 440, 864
AgsI TTSAA 2 cut(s) 110, 209
AjnI CCWGG 2 cut(s) 526, 711
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 1 cut(s) 621
AluI AGCT 1 cut(s) 621
Alw21I GWGCWC 2 cut(s) 623, 1005
AlwI GGATC 1 cut(s) 902
Ama87I CYCGRG 1 cut(s) 351
AoxI GGCC 4 cut(s) 169, 521, 638, 1075
ApeKI GCWGC 1 cut(s) 137
ArsI GACNNNNNNTTYG 2 cut(s) 1000, 1032
Asp700I GAANNNNTTC 1 cut(s) 408
AspS9I GGNCC 2 cut(s) 41, 521
AsuC2I CCSGG 2 cut(s) 352, 353
AsuHPI GGTGA 1 cut(s) 1072
AvaI CYCGRG 1 cut(s) 351
AvaII GGWCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 826
BanII GRGCYC 1 cut(s) 623
Bbv12I GWGCWC 2 cut(s) 623, 1005
BbvI GCAGC 1 cut(s) 149
BccI CCATC 1 cut(s) 1067
BceAI ACGGC 1 cut(s) 470
BciT130I CCWGG 2 cut(s) 528, 713
BcnI CCSGG 2 cut(s) 352, 353
BfaI CTAG 2 cut(s) 383, 435
BfmI CTRYAG 1 cut(s) 186
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 4 cut(s) 352, 353, 528, 713
Bme18I GGWCC 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 351
BmgT120I GGNCC 2 cut(s) 41, 521
BmiI GGNNCC 2 cut(s) 348, 828
BmrFI CCNGG 4 cut(s) 352, 353, 528, 713
BmsI GCATC 4 cut(s) 295, 457, 882, 903
BpmI CTGGAG 1 cut(s) 510
BpuEI CTTGAG 2 cut(s) 479, 520
BpuMI CCSGG 2 cut(s) 352, 353
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 350, 351, 527, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 864
Bse118I RCCGGY 1 cut(s) 978
Bse1I ACTGG 2 cut(s) 547, 823
Bse3DI GCAATG 1 cut(s) 502
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 2 cut(s) 528, 713
BseDI CCNNGG 4 cut(s) 350, 351, 527, 729
BseGI GGATG 4 cut(s) 241, 448, 706, 725
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 440, 864
BseMI GCAATG 1 cut(s) 502
BseNI ACTGG 2 cut(s) 547, 823
BseRI GAGGAG 3 cut(s) 51, 1010, 1101
BseXI GCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 4 cut(s) 171, 523, 640, 1077
BshNI GGYRCC 1 cut(s) 826
BsiHKAI GWGCWC 2 cut(s) 623, 1005
BsiHKCI CYCGRG 1 cut(s) 351
BsiSI CCGG 2 cut(s) 352, 979
BslI CCNNNNNNNGG 2 cut(s) 440, 864
BsnI GGCC 4 cut(s) 171, 523, 640, 1077
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 623, 1005
Bsp143I GATC 3 cut(s) 15, 654, 907
BspACI CCGC 3 cut(s) 23, 507, 811
BspANI GGCC 4 cut(s) 171, 523, 640, 1077
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 2 cut(s) 348, 828
BspPI GGATC 1 cut(s) 902
BspT107I GGYRCC 1 cut(s) 826
BsrBI CCGCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 502
BsrFI RCCGGY 1 cut(s) 978
BsrI ACTGG 2 cut(s) 547, 823
BssAI RCCGGY 1 cut(s) 978
BssECI CCNNGG 4 cut(s) 350, 351, 527, 729
BssMI GATC 3 cut(s) 15, 654, 907
Bst2UI CCWGG 2 cut(s) 528, 713
Bst4CI ACNGT 2 cut(s) 375, 1039
Bst6I CTCTTC 3 cut(s) 504, 1099, 1119
BstC8I GCNNGC 2 cut(s) 284, 942
BstDEI CTNAG 1 cut(s) 632
BstF5I GGATG 4 cut(s) 241, 448, 706, 725
BstKTI GATC 3 cut(s) 18, 657, 910
BstMBI GATC 3 cut(s) 15, 654, 907
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 2 cut(s) 528, 713
BstSCI CCNGG 4 cut(s) 350, 351, 526, 711
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 1073
BsuRI GGCC 4 cut(s) 171, 523, 640, 1077
BtgZI GCGATG 1 cut(s) 830
BtsCI GGATG 4 cut(s) 241, 448, 706, 725
BtsIMutI CAGTG 3 cut(s) 540, 664, 1044
Cac8I GCNNGC 2 cut(s) 284, 942
CciI TCATGA 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 978
Cfr13I GGNCC 2 cut(s) 41, 521
Cfr9I CCCGGG 1 cut(s) 351
CseI GACGC 1 cut(s) 1004
Csp6I GTAC 4 cut(s) 57, 388, 532, 1027
CviAII CATG 3 cut(s) 53, 200, 864
CviJI RGCY 7 cut(s) 171, 523, 621, 640, 711, 765, 1077
CviKI_1 RGCY 7 cut(s) 171, 523, 621, 640, 711, 765, 1077
CviQI GTAC 4 cut(s) 57, 388, 532, 1027
DdeI CTNAG 1 cut(s) 632
DpnI GATC 3 cut(s) 17, 656, 909
DpnII GATC 3 cut(s) 15, 654, 907
DraIII CACNNNGTG 1 cut(s) 1039
Eam1104I CTCTTC 3 cut(s) 504, 1099, 1119
EarI CTCTTC 3 cut(s) 504, 1099, 1119
Ecl136II GAGCTC 1 cut(s) 621
Eco147I AGGCCT 1 cut(s) 640
Eco24I GRGCYC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 621
Eco57I CTGAAG 1 cut(s) 1080
Eco88I CYCGRG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 621
EcoRII CCWGG 2 cut(s) 526, 711
EcoT38I GRGCYC 1 cut(s) 623
FaeI CATG 3 cut(s) 56, 203, 867
FatI CATG 3 cut(s) 52, 199, 863
FauNDI CATATG 1 cut(s) 799
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 4 cut(s) 248, 435, 712, 713
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 2 cut(s) 383, 435
GluI GCNGC 1 cut(s) 138
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 4 cut(s) 171, 523, 640, 1077
HapII CCGG 2 cut(s) 352, 979
HgaI GACGC 1 cut(s) 1004
Hin1II CATG 3 cut(s) 56, 203, 867
HincII GTYRAC 2 cut(s) 517, 883
HindII GTYRAC 2 cut(s) 517, 883
HinfI GANTC 4 cut(s) 196, 257, 326, 1091
HpaII CCGG 2 cut(s) 352, 979
HphI GGTGA 1 cut(s) 1072
Hpy166II GTNNAC 4 cut(s) 186, 517, 883, 1056
Hpy188III TCNNGA 2 cut(s) 200, 743
Hpy8I GTNNAC 4 cut(s) 186, 517, 883, 1056
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 4 cut(s) 78, 556, 574, 630
HpyCH4III ACNGT 2 cut(s) 375, 1039
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 6 cut(s) 137, 218, 596, 609, 803, 916
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpyF3I CTNAG 1 cut(s) 632
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 3 cut(s) 56, 203, 867
Kzo9I GATC 3 cut(s) 15, 654, 907
LmnI GCTCC 3 cut(s) 20, 618, 1008
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 4 cut(s) 295, 457, 882, 903
MaeI CTAG 2 cut(s) 383, 435
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 659
MalI GATC 3 cut(s) 17, 656, 909
MbiI CCGCTC 1 cut(s) 23
MboI GATC 3 cut(s) 15, 654, 907
MhlI GDGCHC 2 cut(s) 623, 1005
MluCI AATT 1 cut(s) 110
MlyI GAGTC 1 cut(s) 1085
MmeI TCCRAC 1 cut(s) 476
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 3 cut(s) 320, 342, 900
MspI CCGG 2 cut(s) 352, 979
MspR9I CCNGG 4 cut(s) 352, 353, 528, 713
MvaI CCWGG 2 cut(s) 528, 713
MwoI GCNNNNNNNGC 1 cut(s) 72
NciI CCSGG 2 cut(s) 352, 353
NdeI CATATG 1 cut(s) 799
NdeII GATC 3 cut(s) 15, 654, 907
NlaIII CATG 3 cut(s) 56, 203, 867
NlaIV GGNNCC 2 cut(s) 348, 828
NmuCI GTSAC 1 cut(s) 659
PagI TCATGA 1 cut(s) 199
PceI AGGCCT 1 cut(s) 640
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 3 cut(s) 196, 257, 326
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 1 cut(s) 1085
PpsI GAGTC 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 623
Psp6I CCWGG 2 cut(s) 526, 711
PspGI CCWGG 2 cut(s) 526, 711
PspN4I GGNNCC 2 cut(s) 348, 828
PspPI GGNCC 2 cut(s) 41, 521
RsaI GTAC 4 cut(s) 58, 389, 533, 1028
RsaNI GTAC 4 cut(s) 57, 388, 532, 1027
SacI GAGCTC 1 cut(s) 623
SaqAI TTAA 3 cut(s) 320, 342, 900
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 3 cut(s) 15, 654, 907
Sau96I GGNCC 2 cut(s) 41, 521
SchI GAGTC 1 cut(s) 1085
ScrFI CCNGG 4 cut(s) 352, 353, 528, 713
SduI GDGCHC 2 cut(s) 623, 1005
SetI ASST 7 cut(s) 89, 315, 436, 594, 623, 828, 1002
SfaNI GCATC 4 cut(s) 295, 457, 882, 903
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 1 cut(s) 41
SmaI CCCGGG 1 cut(s) 353
SmlI CTYRAG 2 cut(s) 458, 535
SmoI CTYRAG 2 cut(s) 458, 535
Sse9I AATT 1 cut(s) 110
SseBI AGGCCT 1 cut(s) 640
SsiI CCGC 3 cut(s) 23, 507, 811
SspMI CTAG 2 cut(s) 383, 435
SstI GAGCTC 1 cut(s) 623
StuI AGGCCT 1 cut(s) 640
StyD4I CCNGG 4 cut(s) 350, 351, 526, 711
TaaI ACNGT 2 cut(s) 375, 1039
TaiI ACGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 263, 924
TasI AATT 1 cut(s) 110
TatI WGTACW 2 cut(s) 387, 1026
TfiI GAWTC 3 cut(s) 196, 257, 326
Tru1I TTAA 3 cut(s) 320, 342, 900
Tru9I TTAA 3 cut(s) 320, 342, 900
TscAI CASTG 3 cut(s) 547, 664, 1044
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 8 cut(s) 148, 270, 377, 444, 491, 575, 593, 717
TspGWI ACGGA 1 cut(s) 306
TspMI CCCGGG 1 cut(s) 351
TspRI CASTG 3 cut(s) 547, 664, 1044
VpaK11BI GGWCC 1 cut(s) 41
XmaI CCCGGG 1 cut(s) 351
XmiI GTMKAC 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 2 cut(s) 383, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.