FvH4_4g32070

Putative small multi-drug export protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011202
Physical Location & Seq
Reverse (-)
30896871 .. 30899493
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32070.t1

Sequence Viewer

Length: 945 bp
ATGGCTGTAGCTCTATCAGCCTCAGCATCTCCATTCACCTCAAAATCGATTCTTTCCAAAACCCTCTTCAGATTTTCACCAAAACACTTCACTCCCCTATCCAAACCACCCCAACTCAATTCAAATACACTCATAAAGCTTCAACCTTTACGCCCATCAATCCCTATTCTCAAAGCTTCTCCTCCACTAGCTTCTTCCCATGGGTTTGAGGAAGATGAGGTCTTGTCAATTTTCGAGGAAAAGCCTGTGAAATTTGGGCTTTGGGTGTTGTTTTGGGCATCTGTTTCACTTGCTTGGTTTGCGGCTTCCGGGGATGCCAATGCAGCTGCCAATGCTGCTGCTGCTGATTCGATCAGAGCTTCTTCTTTTGGTGTGAAGATTGCGAATGCGCTTCGGGGTTTGGGCTGGCCTGATGAGGCTGTGGTGTTTGCATTGGCGACGCTTCCGGTGATTGAGCTTCGTGGGGCTATTCCTGTTGGTTATTGGTTGCAACTCACGCCTGTTATGTTAACTGTTCTAGCTGTTCTTGGGAACATGGTTCCTGTGCCCATCATCATACTTTACTTGAAGAGATTTGCAACTTTCCTTGCTGGAAAGAATAACGCCACATCTCGGTTCCTTGACTTATTGTTTGAAAAGGCTAAAAAGAAAGCTGGCCCTGTAGAGGAGTTCCAATGGCTTGGTCTGATGCTATTTGTGGCTGTGCCTTTCCCGGGAACAGGAGCATGGACAGGAGCCATTATAGCTTCTATTCTCGATATGCCATTTTGGTCGGCAGTGTCTGCAAATTTCTTTGGTGTTGTATTGGCTGGGCTTCTAGTAAATTTGCTGGTGAACCTTGGTCTCAAATATGCTATTGTTACTGGCATTGCTCTCTTCTTTATTTCTACGTTCATGTGGAGCATTCTGCGGAATATTAAGAAGTCCTTAAGCTCATCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sm_multidrug_ex

Protein Analysis

315

Amino Acids

33.81

Weight (kDa)

9.87

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sm_multidrug_ex PF06695 152 - 272 8.3e-39 Putative small multi-drug export protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02590
fragaria_vesca FvH4_4g32070
malus_domestica MD13G1079600.v1.1 MD16G1079500.v1.1
prunus_persica Prupe.1G267200_v2.0.a1
pyrus_communis pycom13g06930 pycom16g06870
rosa_chinensis RchiOBHm_Chr4g0440761
rosa_laevigata RLG00000006156
rosa_multiflora Rmu_co8415195.1_g000001 Rmu_sc0000424.1_g000005 Rmu_sc0022158.1_g000003
rosa_roxburghii Rroxscaffold_5G00381520
rosa_rugosa Rorug04G0327700
rosa_samantha Rh4AG379500 Rh4BG392400 Rh4CG407100 Rh4DG386200
rosa_wichuraiana Rw4G032640 Rw4G032670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 302, 910
AcsI RAATTY 3 cut(s) 251, 787, 823
AcuI CTGAAG 1 cut(s) 52
AfiI CCNNNNNNNGG 4 cut(s) 612, 664, 713, 719
AflII CTTAAG 1 cut(s) 928
AgsI TTSAA 4 cut(s) 123, 143, 568, 635
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
Alw26I GTCTC 1 cut(s) 848
AlwNI CAGNNNCTG 1 cut(s) 782
Ama87I CYCGRG 1 cut(s) 712
AoxI GGCC 2 cut(s) 407, 655
ApeKI GCWGC 5 cut(s) 323, 326, 335, 338, 341
ApoI RAATTY 3 cut(s) 251, 787, 823
ArsI GACNNNNNNTTYG 2 cut(s) 614, 646
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 1 cut(s) 656
AsuC2I CCSGG 3 cut(s) 310, 713, 714
AsuHPI GGTGA 4 cut(s) 28, 69, 460, 844
AvaI CYCGRG 1 cut(s) 712
BaeGI GKGCMC 1 cut(s) 549
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 5 cut(s) 313, 322, 325, 328, 335
BccI CCATC 2 cut(s) 163, 557
BcnI CCSGG 3 cut(s) 310, 713, 714
BcoDI GTCTC 1 cut(s) 848
BfaI CTAG 3 cut(s) 188, 518, 818
BfmI CTRYAG 2 cut(s) 6, 660
BfrI CTTAAG 1 cut(s) 928
BisI GCNGC 6 cut(s) 303, 324, 327, 336, 339, 342
BlsI GCNGC 6 cut(s) 304, 325, 328, 337, 340, 343
Bme1390I CCNGG 3 cut(s) 310, 713, 714
BmeT110I CYCGRG 1 cut(s) 712
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 3 cut(s) 540, 617, 736
BmrFI CCNGG 3 cut(s) 310, 713, 714
BmsI GCATC 4 cut(s) 35, 287, 304, 678
Bpu10I CCTNAGC 1 cut(s) 22
BpuMI CCSGG 3 cut(s) 310, 713, 714
Bsa29I ATCGAT 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 4 cut(s) 199, 309, 712, 838
BsaWI WCCGGW 1 cut(s) 445
Bsc4I CCNNNNNNNGG 4 cut(s) 612, 664, 713, 719
Bse1I ACTGG 1 cut(s) 868
Bse3DI GCAATG 1 cut(s) 867
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 4 cut(s) 199, 309, 712, 838
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 4 cut(s) 612, 664, 713, 719
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 868
BseRI GAGGAG 2 cut(s) 171, 680
BseSI GKGCMC 1 cut(s) 549
BseXI GCAGC 5 cut(s) 313, 322, 325, 328, 335
BseYI CCCAGC 1 cut(s) 809
BshFI GGCC 2 cut(s) 409, 657
BshVI ATCGAT 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 712
BsiSI CCGG 3 cut(s) 309, 446, 713
BslI CCNNNNNNNGG 4 cut(s) 612, 664, 713, 719
BsmAI GTCTC 1 cut(s) 848
BsmI GAATGC 2 cut(s) 391, 903
BsnI GGCC 2 cut(s) 409, 657
Bso31I GGTCTC 1 cut(s) 848
BsoBI CYCGRG 1 cut(s) 712
Bsp1286I GDGCHC 1 cut(s) 549
Bsp143I GATC 1 cut(s) 351
Bsp19I CCATGG 1 cut(s) 199
BspACI CCGC 2 cut(s) 302, 910
BspANI GGCC 2 cut(s) 409, 657
BspCNI CTCAG 1 cut(s) 35
BspDI ATCGAT 1 cut(s) 47
BspLI GGNNCC 3 cut(s) 540, 617, 736
BspTI CTTAAG 1 cut(s) 928
BspTNI GGTCTC 1 cut(s) 848
BsrDI GCAATG 1 cut(s) 867
BsrI ACTGG 1 cut(s) 868
BssECI CCNNGG 4 cut(s) 199, 309, 712, 838
BssMI GATC 1 cut(s) 351
BssT1I CCWWGG 2 cut(s) 199, 838
Bst4CI ACNGT 1 cut(s) 514
Bst6I CTCTTC 3 cut(s) 71, 563, 881
BstAFI CTTAAG 1 cut(s) 928
BstAPI GCANNNNNTGC 1 cut(s) 782
BstC8I GCNNGC 2 cut(s) 407, 655
BstDEI CTNAG 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 199
BstF5I GGATG 1 cut(s) 319
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 1 cut(s) 354
BstMAI GTCTC 1 cut(s) 848
BstMBI GATC 1 cut(s) 351
BstMWI GCNNNNNNNGC 9 cut(s) 17, 299, 323, 332, 335, 341, 496, 743, 782
BstSCI CCNGG 3 cut(s) 308, 711, 712
BstSFI CTRYAG 2 cut(s) 6, 660
BstSLI GKGCMC 1 cut(s) 549
BstV1I GCAGC 5 cut(s) 313, 322, 325, 328, 335
BstXI CCANNNNNNTGG 1 cut(s) 680
Bsu15I ATCGAT 1 cut(s) 47
BsuRI GGCC 2 cut(s) 409, 657
BsuTUI ATCGAT 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 199
BtsCI GGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 1 cut(s) 783
Cac8I GCNNGC 2 cut(s) 407, 655
CaiI CAGNNNCTG 1 cut(s) 782
CfoI GCGC 1 cut(s) 391
Cfr13I GGNCC 1 cut(s) 656
Cfr9I CCCGGG 1 cut(s) 712
ClaI ATCGAT 1 cut(s) 47
CseI GACGC 1 cut(s) 448
CviAII CATG 4 cut(s) 200, 535, 726, 895
DdeI CTNAG 1 cut(s) 22
DpnI GATC 1 cut(s) 353
DpnII GATC 1 cut(s) 351
Eam1104I CTCTTC 3 cut(s) 71, 563, 881
EarI CTCTTC 3 cut(s) 71, 563, 881
Eco130I CCWWGG 2 cut(s) 199, 838
Eco31I GGTCTC 1 cut(s) 848
Eco57I CTGAAG 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 712
EcoT14I CCWWGG 2 cut(s) 199, 838
ErhI CCWWGG 2 cut(s) 199, 838
FaeI CATG 4 cut(s) 203, 538, 729, 898
FalI AAGNNNNNCTT 2 cut(s) 911, 943
FatI CATG 4 cut(s) 199, 534, 725, 894
Fnu4HI GCNGC 6 cut(s) 303, 324, 327, 336, 339, 342
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 6 cut(s) 303, 324, 327, 336, 339, 342
FspBI CTAG 3 cut(s) 188, 518, 818
GlaI GCGC 1 cut(s) 390
GluI GCNGC 6 cut(s) 303, 324, 327, 336, 339, 342
GsaI CCCAGC 1 cut(s) 813
HaeIII GGCC 2 cut(s) 409, 657
HapII CCGG 3 cut(s) 309, 446, 713
HgaI GACGC 1 cut(s) 448
HhaI GCGC 1 cut(s) 391
Hin1II CATG 4 cut(s) 203, 538, 729, 898
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HindIII AAGCTT 2 cut(s) 137, 174
HinfI GANTC 2 cut(s) 49, 347
HpaI GTTAAC 1 cut(s) 510
HpaII CCGG 3 cut(s) 309, 446, 713
HphI GGTGA 4 cut(s) 28, 69, 460, 844
Hpy166II GTNNAC 2 cut(s) 510, 835
Hpy188I TCNGA 3 cut(s) 71, 356, 687
Hpy188III TCNNGA 1 cut(s) 755
Hpy8I GTNNAC 2 cut(s) 510, 835
Hpy99I CGWCG 1 cut(s) 442
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4IV ACGT 1 cut(s) 890
HpyCH4V TGCA 5 cut(s) 323, 431, 490, 578, 785
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 299, 323, 332, 335, 341, 496, 743, 782
HpyF3I CTNAG 1 cut(s) 22
HpySE526I ACGT 1 cut(s) 890
Hsp92II CATG 4 cut(s) 203, 538, 729, 898
HspAI GCGC 1 cut(s) 389
KspAI GTTAAC 1 cut(s) 510
Kzo9I GATC 1 cut(s) 351
LmnI GCTCC 3 cut(s) 722, 734, 900
Lsp1109I GCAGC 5 cut(s) 313, 322, 325, 328, 335
LweI GCATC 4 cut(s) 35, 287, 304, 678
MaeI CTAG 3 cut(s) 188, 518, 818
MaeII ACGT 1 cut(s) 890
MaeIII GTNAC 1 cut(s) 859
MalI GATC 1 cut(s) 353
MboI GATC 1 cut(s) 351
MboII GAAGA 7 cut(s) 58, 186, 224, 354, 388, 580, 868
MhlI GDGCHC 1 cut(s) 549
MluCI AATT 5 cut(s) 118, 228, 251, 787, 823
MnlI CCTC 9 cut(s) 31, 49, 74, 192, 202, 211, 229, 409, 658
MseI TTAA 3 cut(s) 509, 918, 929
MspA1I CMGCKG 1 cut(s) 326
MspCI CTTAAG 1 cut(s) 928
MspI CCGG 3 cut(s) 309, 446, 713
MspR9I CCNGG 3 cut(s) 310, 713, 714
Mva1269I GAATGC 2 cut(s) 391, 903
MwoI GCNNNNNNNGC 9 cut(s) 17, 299, 323, 332, 335, 341, 496, 743, 782
NciI CCSGG 3 cut(s) 310, 713, 714
NcoI CCATGG 1 cut(s) 199
NdeII GATC 1 cut(s) 351
NlaIII CATG 4 cut(s) 203, 538, 729, 898
NlaIV GGNNCC 3 cut(s) 540, 617, 736
PctI GAATGC 2 cut(s) 391, 903
PfeI GAWTC 2 cut(s) 49, 347
PkrI GCNGC 6 cut(s) 304, 325, 328, 337, 340, 343
PspFI CCCAGC 1 cut(s) 809
PspN4I GGNNCC 3 cut(s) 540, 617, 736
PspPI GGNCC 1 cut(s) 656
PstNI CAGNNNCTG 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 326
SaqAI TTAA 3 cut(s) 509, 918, 929
SatI GCNGC 6 cut(s) 303, 324, 327, 336, 339, 342
Sau3AI GATC 1 cut(s) 351
Sau96I GGNCC 1 cut(s) 656
ScrFI CCNGG 3 cut(s) 310, 713, 714
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 4 cut(s) 35, 287, 304, 678
SfcI CTRYAG 2 cut(s) 6, 660
SmaI CCCGGG 1 cut(s) 714
SmlI CTYRAG 1 cut(s) 928
SmoI CTYRAG 1 cut(s) 928
Sse9I AATT 5 cut(s) 118, 228, 251, 787, 823
SsiI CCGC 2 cut(s) 302, 910
SspI AATATT 1 cut(s) 916
SspMI CTAG 3 cut(s) 188, 518, 818
StyD4I CCNGG 3 cut(s) 308, 711, 712
StyI CCWWGG 2 cut(s) 199, 838
TaaI ACNGT 1 cut(s) 514
TaiI ACGT 1 cut(s) 893
TaqI TCGA 4 cut(s) 47, 234, 350, 756
TasI AATT 5 cut(s) 118, 228, 251, 787, 823
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 2 cut(s) 49, 347
Tru1I TTAA 3 cut(s) 509, 918, 929
Tru9I TTAA 3 cut(s) 509, 918, 929
TscAI CASTG 1 cut(s) 783
TseI GCWGC 5 cut(s) 323, 326, 335, 338, 341
TspDTI ATGAA 1 cut(s) 883
TspMI CCCGGG 1 cut(s) 712
TspRI CASTG 1 cut(s) 783
Vha464I CTTAAG 1 cut(s) 928
XapI RAATTY 3 cut(s) 251, 787, 823
XmaI CCCGGG 1 cut(s) 712
XspI CTAG 3 cut(s) 188, 518, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.