Rroxscaffold_5G00381520

Putative small multi-drug export protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011202
Physical Location & Seq
Reverse (-)
61467491 .. 61469682
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00381520.1

Sequence Viewer

Length: 762 bp
ATGGCTGTAACTCTATCAGCCTCAGCATCTCCATTAACATCAAAATTGATTCTTGGCAAAACCCTCCTCAGATTTTCACCAAAACACTCCCACCCTTTCACTTCCCAAACCAAACAACCCCCATTCAATCCAAATGGACTCATAAAGCTTCAATCTTTACGCCCATTAGTCCCTATTCTGAAAGCTTCTCCTCCACGAGCTTCTTCAAATGGGTTTGAGGAAGATGAGGTCTTGTCAATTTTCCAAGAACAGCCGGTCAAATTTGGGCTTTGGGTGTTGCTTTGGGCCTCTGTTTCACTTGCTTGGTTTGCTGCTTCCGGGGATGCCAATGCAGCTGCCACTGCCACTGCTGCTGCTGCTGCTGATTCGATTAGAGCTTCCTCTTTCGGCCTGAAGATTGCGAATGGGCTTCGGGGTTTGGGCTGGCCTGATGAGGCTGTGGTGTTTGCATTGGCTACGCTTCCGGTGATTGAGCTTCGTGGGGCTATTCCTGTTGGTTATTGGTTGCAACTCAAGCCTGTTATGCTAACTGTTCTAGCTGTTCTTGGGAACATGGTTCCTGTGCCCTTCATCATACTTTACTTGAAGAGATTTGCATCTTTCCTTGCTGGAAAGAATAAAGCTGCATCTCAGTTTCTTGACTTATTGTTTGAGAAGGCTAAAAAGAAAGCTGGCCCTGTAGAGGAGTTCCAATGGCTTGGTCTGATGCTGTTTGTGGCTGTGCCTTTCCGGGAACAGGAGCATGGACAGGAGCCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sm_multidrug_ex

Protein Analysis

253

Amino Acids

27.36

Weight (kDa)

9.69

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sm_multidrug_ex PF06695 158 - 243 3.2e-22 Putative small multi-drug export protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02590
fragaria_vesca FvH4_4g32070
malus_domestica MD13G1079600.v1.1 MD16G1079500.v1.1
prunus_persica Prupe.1G267200_v2.0.a1
pyrus_communis pycom13g06930 pycom16g06870
rosa_chinensis RchiOBHm_Chr4g0440761
rosa_laevigata RLG00000006156
rosa_multiflora Rmu_co8415195.1_g000001 Rmu_sc0000424.1_g000005 Rmu_sc0022158.1_g000003
rosa_roxburghii Rroxscaffold_5G00381520
rosa_rugosa Rorug04G0327700
rosa_samantha Rh4AG379500 Rh4BG392400 Rh4CG407100 Rh4DG386200
rosa_wichuraiana Rw4G032640 Rw4G032670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 413
AfiI CCNNNNNNNGG 2 cut(s) 682, 736
AgsI TTSAA 4 cut(s) 127, 152, 207, 586
AluBI AGCT 9 cut(s) 148, 185, 200, 335, 377, 475, 539, 623, 671
AluI AGCT 9 cut(s) 148, 185, 200, 335, 377, 475, 539, 623, 671
AoxI GGCC 4 cut(s) 285, 388, 425, 673
ApeKI GCWGC 8 cut(s) 311, 332, 335, 350, 353, 356, 359, 623
ApoI RAATTY 1 cut(s) 260
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspS9I GGNCC 2 cut(s) 285, 674
AsuC2I CCSGG 2 cut(s) 319, 731
AsuHPI GGTGA 2 cut(s) 69, 478
BaeGI GKGCMC 1 cut(s) 567
BauI CACGAG 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 8 cut(s) 298, 322, 337, 340, 343, 344, 346, 610
BcnI CCSGG 2 cut(s) 319, 731
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 678
BisI GCNGC 8 cut(s) 312, 333, 336, 351, 354, 357, 360, 624
BlsI GCNGC 8 cut(s) 313, 334, 337, 352, 355, 358, 361, 625
Bme1390I CCNGG 2 cut(s) 319, 731
BmgT120I GGNCC 2 cut(s) 285, 674
BmiI GGNNCC 2 cut(s) 558, 753
BmrFI CCNGG 2 cut(s) 319, 731
BmsI GCATC 5 cut(s) 35, 313, 605, 635, 696
Bpu10I CCTNAGC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 2 cut(s) 319, 731
BsaBI GATNNNNATC 1 cut(s) 595
BsaJI CCNNGG 1 cut(s) 318
BsaWI WCCGGW 1 cut(s) 463
BsaXI ACNNNNNCTCC 1 cut(s) 743
Bsc4I CCNNNNNNNGG 2 cut(s) 682, 736
Bse118I RCCGGY 1 cut(s) 253
Bse8I GATNNNNATC 1 cut(s) 595
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 1 cut(s) 328
BseJI GATNNNNATC 1 cut(s) 595
BseLI CCNNNNNNNGG 2 cut(s) 682, 736
BseMII CTCAG 3 cut(s) 36, 82, 644
BseRI GAGGAG 3 cut(s) 56, 180, 698
BseSI GKGCMC 1 cut(s) 567
BseXI GCAGC 8 cut(s) 298, 322, 337, 340, 343, 344, 346, 610
BshFI GGCC 4 cut(s) 287, 390, 427, 675
BsiSI CCGG 4 cut(s) 254, 318, 464, 730
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 2 cut(s) 682, 736
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 4 cut(s) 287, 390, 427, 675
Bsp1286I GDGCHC 1 cut(s) 567
BspANI GGCC 4 cut(s) 287, 390, 427, 675
BspCNI CTCAG 3 cut(s) 35, 81, 643
BspLI GGNNCC 2 cut(s) 558, 753
BsrFI RCCGGY 1 cut(s) 253
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 1 cut(s) 318
BssSI CACGAG 1 cut(s) 195
Bst2BI CACGAG 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 532
Bst6I CTCTTC 1 cut(s) 581
BstC8I GCNNGC 2 cut(s) 425, 673
BstDEI CTNAG 3 cut(s) 22, 68, 630
BstF5I GGATG 1 cut(s) 328
BstMWI GCNNNNNNNGC 8 cut(s) 308, 332, 341, 350, 356, 359, 514, 523
BstSCI CCNGG 2 cut(s) 317, 729
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 1 cut(s) 567
BstV1I GCAGC 8 cut(s) 298, 322, 337, 340, 343, 344, 346, 610
BstXI CCANNNNNNTGG 1 cut(s) 698
BsuRI GGCC 4 cut(s) 287, 390, 427, 675
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 2 cut(s) 339, 345
BtsIMutI CAGTG 2 cut(s) 339, 345
Cac8I GCNNGC 2 cut(s) 425, 673
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 285, 674
CviAII CATG 2 cut(s) 553, 743
DdeI CTNAG 3 cut(s) 22, 68, 630
Eam1104I CTCTTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 581
Eco57I CTGAAG 1 cut(s) 413
FaeI CATG 2 cut(s) 556, 746
FaiI YATR 5 cut(s) 143, 524, 554, 575, 744
FaqI GGGAC 1 cut(s) 155
FatI CATG 2 cut(s) 552, 742
Fnu4HI GCNGC 8 cut(s) 312, 333, 336, 351, 354, 357, 360, 624
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 8 cut(s) 312, 333, 336, 351, 354, 357, 360, 624
FspBI CTAG 1 cut(s) 536
GluI GCNGC 8 cut(s) 312, 333, 336, 351, 354, 357, 360, 624
HaeIII GGCC 4 cut(s) 287, 390, 427, 675
HapII CCGG 4 cut(s) 254, 318, 464, 730
Hin1II CATG 2 cut(s) 556, 746
HindIII AAGCTT 2 cut(s) 146, 183
HinfI GANTC 3 cut(s) 49, 138, 365
HpaII CCGG 4 cut(s) 254, 318, 464, 730
HphI GGTGA 2 cut(s) 69, 478
Hpy188I TCNGA 3 cut(s) 71, 180, 705
Hpy188III TCNNGA 1 cut(s) 638
HpyAV CCTTC 2 cut(s) 577, 649
HpyCH4III ACNGT 1 cut(s) 532
HpyCH4V TGCA 5 cut(s) 332, 449, 508, 596, 626
HpyF10VI GCNNNNNNNGC 8 cut(s) 308, 332, 341, 350, 356, 359, 514, 523
HpyF3I CTNAG 3 cut(s) 22, 68, 630
Hsp92II CATG 2 cut(s) 556, 746
LmnI GCTCC 2 cut(s) 739, 751
Lsp1109I GCAGC 8 cut(s) 298, 322, 337, 340, 343, 344, 346, 610
LweI GCATC 5 cut(s) 35, 313, 605, 635, 696
MaeI CTAG 1 cut(s) 536
MaeIII GTNAC 1 cut(s) 7
MboII GAAGA 4 cut(s) 195, 233, 406, 598
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 3 cut(s) 44, 237, 260
MlyI GAGTC 1 cut(s) 132
MseI TTAA 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 4 cut(s) 254, 318, 464, 730
MspR9I CCNGG 2 cut(s) 319, 731
MwoI GCNNNNNNNGC 8 cut(s) 308, 332, 341, 350, 356, 359, 514, 523
NciI CCSGG 2 cut(s) 319, 731
NlaIII CATG 2 cut(s) 556, 746
NlaIV GGNNCC 2 cut(s) 558, 753
PfeI GAWTC 2 cut(s) 49, 365
PfoI TCCNGGA 1 cut(s) 729
PkrI GCNGC 8 cut(s) 313, 334, 337, 352, 355, 358, 361, 625
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PspN4I GGNNCC 2 cut(s) 558, 753
PspPI GGNCC 2 cut(s) 285, 674
PvuII CAGCTG 1 cut(s) 335
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 8 cut(s) 312, 333, 336, 351, 354, 357, 360, 624
Sau96I GGNCC 2 cut(s) 285, 674
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 2 cut(s) 319, 731
SduI GDGCHC 1 cut(s) 567
SfaNI GCATC 5 cut(s) 35, 313, 605, 635, 696
SfcI CTRYAG 1 cut(s) 678
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 3 cut(s) 44, 237, 260
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 317, 729
TaaI ACNGT 1 cut(s) 532
TaqI TCGA 1 cut(s) 368
TasI AATT 3 cut(s) 44, 237, 260
TfiI GAWTC 2 cut(s) 49, 365
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 2 cut(s) 346, 352
TseI GCWGC 8 cut(s) 311, 332, 335, 350, 353, 356, 359, 623
TspDTI ATGAA 1 cut(s) 559
TspRI CASTG 2 cut(s) 346, 352
XapI RAATTY 1 cut(s) 260
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.