RLG00000006156

Putative small multi-drug export protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011202
Physical Location & Seq
Forward (+)
4802554 .. 4804054
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006156

Sequence Viewer

Length: 948 bp
ATGGCTGTAACTCTATCAGCCTCAGCATCTCCATTAACATCAAAATTGATTCTTGGCAAAACCCTCCTCAGATTTTCACCAAAACACTCCCACTCTTTCACTCCCCAAACCAAACAACCCCCATTCAATCCAAACGGACTCATAAAGCTTCAATCTTTACGCCCATTAGTCCCTATTCTGAAAGCGTCTCCTCCACGAGCTTCTTCAAATGAGTTTGAGGAAGATGAGGTCTTGTCAATTTTCCAAGAACGGCCGGTCAAATTTGGGCTTTGGGTGTTGCTTTGGGCCTCTGTTTCACTTGCTTGGTTTGCTGCTTCCGGGGATGCCAATGCAGCTGCCACTGCTGCTGATTCGATTAGAGCTTCCTCTTTCGGCCTGAAGATTGCAAATTGGCTTCGCGGTTTGGGCTGGCCTGATGAGGCTGTGGTGTTTGCCCTTGCTACGCTTCCGGTGATTGAGCTTCGTGGGGCTATTCCTGTTGGTTATTGGTTGCAACTCAAGCCTGTTATGCTAACTGTTCTAGCTGTTCTTGGGAACATGGTTCCTGTGCCCTTCATCATACTTTACTTGAAGAGATTTGCATCTCTCCTTGCTGGAAAGAATAAAGCTGCATCTCAGTTCCTTGACTTATTGTTTGAGAAGGCTAAAAAGAAAGCTGGCCCTGTAGAGGAGTTCCAATGGCTTGGTCTGATGCTGTTTGTGGCTGTGCCTTTCCCGGGAACAGGAGCATGGACAGGAGCCATTGTAGCTTCTATTCTTGATATGCCATTTTGGTCAGCAGTGTCCGCAAATTTCTTTGGTGTTGTATTGGCTGGGCTTCTAGTGAATTTGCTGGTGAACCTTGGTCTCAAATATGCTATTGTTACTGGTATTGTTCTCTTCTTTATCTCTACATTCATGTGGAGCATCCTTCGGAATCTTAAGAAGTCCTTAAGCTCATCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sm_multidrug_ex

Protein Analysis

316

Amino Acids

34.16

Weight (kDa)

9.99

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sm_multidrug_ex PF06695 153 - 273 4.1e-39 Putative small multi-drug export protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02590
fragaria_vesca FvH4_4g32070
malus_domestica MD13G1079600.v1.1 MD16G1079500.v1.1
prunus_persica Prupe.1G267200_v2.0.a1
pyrus_communis pycom13g06930 pycom16g06870
rosa_chinensis RchiOBHm_Chr4g0440761
rosa_laevigata RLG00000006156
rosa_multiflora Rmu_co8415195.1_g000001 Rmu_sc0000424.1_g000005 Rmu_sc0022158.1_g000003
rosa_roxburghii Rroxscaffold_5G00381520
rosa_rugosa Rorug04G0327700
rosa_samantha Rh4AG379500 Rh4BG392400 Rh4CG407100 Rh4DG386200
rosa_wichuraiana Rw4G032640 Rw4G032670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 399
AciI CCGC 2 cut(s) 399, 786
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 3 cut(s) 260, 790, 826
AcuI CTGAAG 1 cut(s) 398
AfiI CCNNNNNNNGG 3 cut(s) 667, 716, 722
AflII CTTAAG 2 cut(s) 920, 931
AgsI TTSAA 4 cut(s) 127, 152, 207, 571
Alw26I GTCTC 2 cut(s) 192, 851
Ama87I CYCGRG 1 cut(s) 715
AoxI GGCC 5 cut(s) 251, 285, 373, 410, 658
ApeKI GCWGC 5 cut(s) 311, 332, 335, 344, 608
ApoI RAATTY 3 cut(s) 260, 790, 826
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
AspS9I GGNCC 2 cut(s) 285, 659
AsuC2I CCSGG 3 cut(s) 319, 716, 717
AsuHPI GGTGA 3 cut(s) 69, 463, 847
AvaI CYCGRG 1 cut(s) 715
BaeGI GKGCMC 1 cut(s) 552
BauI CACGAG 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 5 cut(s) 298, 322, 331, 344, 595
BceAI ACGGC 1 cut(s) 266
BcnI CCSGG 3 cut(s) 319, 716, 717
BcoDI GTCTC 2 cut(s) 192, 851
BfaI CTAG 2 cut(s) 521, 821
BfmI CTRYAG 1 cut(s) 663
BfrI CTTAAG 2 cut(s) 920, 931
BisI GCNGC 5 cut(s) 312, 333, 336, 345, 609
BlsI GCNGC 5 cut(s) 313, 334, 337, 346, 610
Bme1390I CCNGG 3 cut(s) 319, 716, 717
BmeT110I CYCGRG 1 cut(s) 715
BmgT120I GGNCC 2 cut(s) 285, 659
BmiI GGNNCC 2 cut(s) 543, 739
BmrFI CCNGG 3 cut(s) 319, 716, 717
BmsI GCATC 6 cut(s) 35, 313, 590, 620, 681, 915
Bpu10I CCTNAGC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 3 cut(s) 319, 716, 717
BsaBI GATNNNNATC 1 cut(s) 580
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 3 cut(s) 318, 715, 841
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 667, 716, 722
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 871
Bse8I GATNNNNATC 1 cut(s) 580
BseDI CCNNGG 3 cut(s) 318, 715, 841
BseGI GGATG 2 cut(s) 328, 906
BseJI GATNNNNATC 1 cut(s) 580
BseLI CCNNNNNNNGG 3 cut(s) 667, 716, 722
BseMII CTCAG 3 cut(s) 36, 82, 629
BseNI ACTGG 1 cut(s) 871
BseRI GAGGAG 3 cut(s) 56, 180, 683
BseSI GKGCMC 1 cut(s) 552
BseX3I CGGCCG 1 cut(s) 251
BseXI GCAGC 5 cut(s) 298, 322, 331, 344, 595
BseYI CCCAGC 1 cut(s) 812
Bsh1236I CGCG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 254
BshFI GGCC 5 cut(s) 253, 287, 375, 412, 660
BsiEI CGRYCG 1 cut(s) 254
BsiHKCI CYCGRG 1 cut(s) 715
BsiSI CCGG 4 cut(s) 254, 318, 449, 716
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 3 cut(s) 667, 716, 722
BsmAI GTCTC 2 cut(s) 192, 851
BsmBI CGTCTC 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 5 cut(s) 253, 287, 375, 412, 660
Bso31I GGTCTC 1 cut(s) 851
BsoBI CYCGRG 1 cut(s) 715
Bsp1286I GDGCHC 1 cut(s) 552
BspACI CCGC 2 cut(s) 399, 786
BspANI GGCC 5 cut(s) 253, 287, 375, 412, 660
BspCNI CTCAG 3 cut(s) 35, 81, 628
BspFNI CGCG 1 cut(s) 399
BspLI GGNNCC 2 cut(s) 543, 739
BspTI CTTAAG 2 cut(s) 920, 931
BspTNI GGTCTC 1 cut(s) 851
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 1 cut(s) 871
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 3 cut(s) 318, 715, 841
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 841
Bst2BI CACGAG 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 517
Bst6I CTCTTC 2 cut(s) 566, 884
BstAFI CTTAAG 2 cut(s) 920, 931
BstC8I GCNNGC 2 cut(s) 410, 658
BstDEI CTNAG 3 cut(s) 22, 68, 615
BstF5I GGATG 2 cut(s) 328, 906
BstFNI CGCG 1 cut(s) 399
BstMAI GTCTC 2 cut(s) 192, 851
BstMCI CGRYCG 1 cut(s) 254
BstMWI GCNNNNNNNGC 9 cut(s) 308, 332, 341, 344, 405, 499, 508, 746, 785
BstSCI CCNGG 3 cut(s) 317, 714, 715
BstSFI CTRYAG 1 cut(s) 663
BstSLI GKGCMC 1 cut(s) 552
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 5 cut(s) 298, 322, 331, 344, 595
BstXI CCANNNNNNTGG 1 cut(s) 683
BstZI CGGCCG 1 cut(s) 251
BsuRI GGCC 5 cut(s) 253, 287, 375, 412, 660
BtsCI GGATG 2 cut(s) 328, 906
BtsI GCAGTG 2 cut(s) 339, 786
BtsIMutI CAGTG 2 cut(s) 339, 786
Cac8I GCNNGC 2 cut(s) 410, 658
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 285, 659
Cfr9I CCCGGG 1 cut(s) 715
CseI GACGC 1 cut(s) 174
CviAII CATG 3 cut(s) 538, 729, 898
DdeI CTNAG 3 cut(s) 22, 68, 615
EaeI YGGCCR 1 cut(s) 251
EagI CGGCCG 1 cut(s) 251
Eam1104I CTCTTC 2 cut(s) 566, 884
EarI CTCTTC 2 cut(s) 566, 884
EclXI CGGCCG 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 841
Eco31I GGTCTC 1 cut(s) 851
Eco52I CGGCCG 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 398
Eco88I CYCGRG 1 cut(s) 715
EcoT14I CCWWGG 1 cut(s) 841
ErhI CCWWGG 1 cut(s) 841
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 3 cut(s) 541, 732, 901
FaiI YATR 8 cut(s) 143, 509, 539, 560, 730, 764, 855, 899
FalI AAGNNNNNCTT 2 cut(s) 914, 946
FaqI GGGAC 1 cut(s) 155
FatI CATG 3 cut(s) 537, 728, 897
Fnu4HI GCNGC 5 cut(s) 312, 333, 336, 345, 609
FokI GGATG 2 cut(s) 335, 893
Fsp4HI GCNGC 5 cut(s) 312, 333, 336, 345, 609
FspBI CTAG 2 cut(s) 521, 821
GluI GCNGC 5 cut(s) 312, 333, 336, 345, 609
GsaI CCCAGC 1 cut(s) 816
HaeIII GGCC 5 cut(s) 253, 287, 375, 412, 660
HapII CCGG 4 cut(s) 254, 318, 449, 716
HgaI GACGC 1 cut(s) 174
Hin1II CATG 3 cut(s) 541, 732, 901
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 4 cut(s) 49, 138, 350, 916
HpaII CCGG 4 cut(s) 254, 318, 449, 716
HphI GGTGA 3 cut(s) 69, 463, 847
Hpy166II GTNNAC 1 cut(s) 838
Hpy188I TCNGA 4 cut(s) 71, 180, 690, 915
Hpy188III TCNNGA 1 cut(s) 758
Hpy8I GTNNAC 1 cut(s) 838
HpyAV CCTTC 3 cut(s) 562, 634, 920
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4V TGCA 5 cut(s) 332, 386, 493, 581, 611
HpyF10VI GCNNNNNNNGC 9 cut(s) 308, 332, 341, 344, 405, 499, 508, 746, 785
HpyF3I CTNAG 3 cut(s) 22, 68, 615
Hsp92II CATG 3 cut(s) 541, 732, 901
LmnI GCTCC 3 cut(s) 725, 737, 903
Lsp1109I GCAGC 5 cut(s) 298, 322, 331, 344, 595
LweI GCATC 6 cut(s) 35, 313, 590, 620, 681, 915
MaeI CTAG 2 cut(s) 521, 821
MaeIII GTNAC 2 cut(s) 7, 862
MboII GAAGA 5 cut(s) 195, 233, 391, 583, 871
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 6 cut(s) 44, 237, 260, 388, 790, 826
MlyI GAGTC 1 cut(s) 132
MseI TTAA 3 cut(s) 35, 921, 932
MslI CAYNNNNRTG 1 cut(s) 898
MspA1I CMGCKG 1 cut(s) 335
MspCI CTTAAG 2 cut(s) 920, 931
MspI CCGG 4 cut(s) 254, 318, 449, 716
MspR9I CCNGG 3 cut(s) 319, 716, 717
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 9 cut(s) 308, 332, 341, 344, 405, 499, 508, 746, 785
NciI CCSGG 3 cut(s) 319, 716, 717
NlaIII CATG 3 cut(s) 541, 732, 901
NlaIV GGNNCC 2 cut(s) 543, 739
PfeI GAWTC 3 cut(s) 49, 350, 916
PkrI GCNGC 5 cut(s) 313, 334, 337, 346, 610
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 812
PspN4I GGNNCC 2 cut(s) 543, 739
PspPI GGNCC 2 cut(s) 285, 659
PvuII CAGCTG 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 898
SaqAI TTAA 3 cut(s) 35, 921, 932
SatI GCNGC 5 cut(s) 312, 333, 336, 345, 609
Sau96I GGNCC 2 cut(s) 285, 659
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 3 cut(s) 319, 716, 717
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 6 cut(s) 35, 313, 590, 620, 681, 915
SfcI CTRYAG 1 cut(s) 663
SmaI CCCGGG 1 cut(s) 717
SmiMI CAYNNNNRTG 1 cut(s) 898
SmlI CTYRAG 3 cut(s) 497, 920, 931
SmoI CTYRAG 3 cut(s) 497, 920, 931
Sse9I AATT 6 cut(s) 44, 237, 260, 388, 790, 826
SsiI CCGC 2 cut(s) 399, 786
SspMI CTAG 2 cut(s) 521, 821
StyD4I CCNGG 3 cut(s) 317, 714, 715
StyI CCWWGG 1 cut(s) 841
TaaI ACNGT 1 cut(s) 517
TaqI TCGA 1 cut(s) 353
TasI AATT 6 cut(s) 44, 237, 260, 388, 790, 826
TfiI GAWTC 3 cut(s) 49, 350, 916
Tru1I TTAA 3 cut(s) 35, 921, 932
Tru9I TTAA 3 cut(s) 35, 921, 932
TscAI CASTG 2 cut(s) 346, 786
TseI GCWGC 5 cut(s) 311, 332, 335, 344, 608
TspDTI ATGAA 2 cut(s) 544, 886
TspGWI ACGGA 1 cut(s) 150
TspMI CCCGGG 1 cut(s) 715
TspRI CASTG 2 cut(s) 346, 786
Vha464I CTTAAG 2 cut(s) 920, 931
XapI RAATTY 3 cut(s) 260, 790, 826
XmaI CCCGGG 1 cut(s) 715
XspI CTAG 2 cut(s) 521, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.