Rh4DG386200

Putative small multi-drug export protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011202
Physical Location & Seq
Reverse (-)
60314786 .. 60317126
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG386200.1

Sequence Viewer

Length: 957 bp
ATGGCTGTAACTCTATCAGCCTCAGCATCTCCATTAACATCAAAATTGATTCTTGGCAAAACCCTCCTCAGATTTTCACCAAAACACTCCCACCCTTTCACTCCCCAAACCAAACAACCCCCATTCAATCCAAATGGACTCATAAAGCTTCAATCTTTACGCCCAGTAGTCCCTATTCTGAAAGCTTCTCCTCCACGAGCTTCTTCAAATGGGTTTGAGGAAGATGAGGTCTTGTCAATTTTCCAAGAACGGCCGGTCAAATTTGGGCTTTGGGTGTTGCTTTGGGCCTCTGTTTCACTTGCTTGGTTTGCTGCTTCCGGGGATGCCAATGCAGCTGCCACTGCTGCTGCTGCTGCTGATTCGATTAGAGCTTCCTCTTTCGGCCTGAAGATTGCGAATGGGCTTCGGGGTTTGGGCTGGCCTGATGAGGCTGTGGTGTTTGCATTGGCTACGCTTCCGGTGATTGAGCTTCGTGGGGCTATTCCTGTTGGTTATTGGTTGCAACTCAAGCCTGTTATGCTAACTGTTCTAGCTGTTCTTGGGAACATGGTTCCTGTGCCCTTCATCATACTTTACTTGAAGAGATTTGCATCTTTCCTTGCTGGAAAGAATAAAGCTGCATCTCAGTTCCTTGACTTACTGTTTGAGAAGGCTAAAAAGAAAGCTGGCCCTGTAGAGGAGTTCCAATGGCTTGGTCTGATGCTGTTTGTGGCTGTGCCTTTCCCGGGAACAGGAGCATGGACAGGAGCCATTGTAGCTTCTATTCTTGATATGCCATTTTGGTTAGCAGTGTCCGCAAATTTCTTTGGTGTTGTGTTGGCTGGGCTTCTAGTGAATTTGCTGGTGAACCTTGGTCTCAGATATGCTATTGTTACCGGTATTGTTCTCTTCTTTATCTCTACGTTCATGTGGAGCATCCTTCGGAATCTTAAGAAGTCCTTAAGCTCATCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sm_multidrug_ex

Protein Analysis

318

Amino Acids

34.25

Weight (kDa)

10.11

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sm_multidrug_ex PF06695 156 - 276 1.6e-38 Putative small multi-drug export protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02590
fragaria_vesca FvH4_4g32070
malus_domestica MD13G1079600.v1.1 MD16G1079500.v1.1
prunus_persica Prupe.1G267200_v2.0.a1
pyrus_communis pycom13g06930 pycom16g06870
rosa_chinensis RchiOBHm_Chr4g0440761
rosa_laevigata RLG00000006156
rosa_multiflora Rmu_co8415195.1_g000001 Rmu_sc0000424.1_g000005 Rmu_sc0022158.1_g000003
rosa_roxburghii Rroxscaffold_5G00381520
rosa_rugosa Rorug04G0327700
rosa_samantha Rh4AG379500 Rh4BG392400 Rh4CG407100 Rh4DG386200
rosa_wichuraiana Rw4G032640 Rw4G032670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 795
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 3 cut(s) 260, 799, 835
AcuI CTGAAG 1 cut(s) 407
AfiI CCNNNNNNNGG 3 cut(s) 676, 725, 731
AflII CTTAAG 2 cut(s) 929, 940
AgeI ACCGGT 1 cut(s) 875
AgsI TTSAA 4 cut(s) 127, 152, 207, 580
Alw26I GTCTC 1 cut(s) 860
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 5 cut(s) 251, 285, 382, 419, 667
ApeKI GCWGC 8 cut(s) 311, 332, 335, 344, 347, 350, 353, 617
ApoI RAATTY 3 cut(s) 260, 799, 835
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
AsiGI ACCGGT 1 cut(s) 875
AspS9I GGNCC 2 cut(s) 285, 668
AsuC2I CCSGG 3 cut(s) 319, 725, 726
AsuHPI GGTGA 3 cut(s) 69, 472, 856
AvaI CYCGRG 1 cut(s) 724
BaeGI GKGCMC 1 cut(s) 561
BauI CACGAG 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 8 cut(s) 298, 322, 331, 334, 337, 340, 344, 604
BceAI ACGGC 1 cut(s) 266
BcnI CCSGG 3 cut(s) 319, 725, 726
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 2 cut(s) 530, 830
BfmI CTRYAG 1 cut(s) 672
BfrI CTTAAG 2 cut(s) 929, 940
BisI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 354, 618
BlsI GCNGC 8 cut(s) 313, 334, 337, 346, 349, 352, 355, 619
Bme1390I CCNGG 3 cut(s) 319, 725, 726
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 285, 668
BmiI GGNNCC 2 cut(s) 552, 748
BmrFI CCNGG 3 cut(s) 319, 725, 726
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 6 cut(s) 35, 313, 599, 629, 690, 924
BmuI ACTGGG 1 cut(s) 158
Bpu10I CCTNAGC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 3 cut(s) 319, 725, 726
BsaBI GATNNNNATC 1 cut(s) 589
BsaI GGTCTC 1 cut(s) 860
BsaJI CCNNGG 3 cut(s) 318, 724, 850
BsaWI WCCGGW 2 cut(s) 457, 875
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 3 cut(s) 676, 725, 731
Bse118I RCCGGY 2 cut(s) 253, 875
Bse1I ACTGG 1 cut(s) 164
Bse8I GATNNNNATC 1 cut(s) 589
BseDI CCNNGG 3 cut(s) 318, 724, 850
BseGI GGATG 2 cut(s) 328, 915
BseJI GATNNNNATC 1 cut(s) 589
BseLI CCNNNNNNNGG 3 cut(s) 676, 725, 731
BseMII CTCAG 4 cut(s) 36, 82, 638, 871
BseNI ACTGG 1 cut(s) 164
BseRI GAGGAG 3 cut(s) 56, 180, 692
BseSI GKGCMC 1 cut(s) 561
BseX3I CGGCCG 1 cut(s) 251
BseXI GCAGC 8 cut(s) 298, 322, 331, 334, 337, 340, 344, 604
BseYI CCCAGC 1 cut(s) 821
Bsh1285I CGRYCG 1 cut(s) 254
BshFI GGCC 5 cut(s) 253, 287, 384, 421, 669
BshTI ACCGGT 1 cut(s) 875
BsiEI CGRYCG 1 cut(s) 254
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 5 cut(s) 254, 318, 458, 725, 876
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 3 cut(s) 676, 725, 731
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 5 cut(s) 253, 287, 384, 421, 669
Bso31I GGTCTC 1 cut(s) 860
BsoBI CYCGRG 1 cut(s) 724
Bsp1286I GDGCHC 1 cut(s) 561
BspACI CCGC 1 cut(s) 795
BspANI GGCC 5 cut(s) 253, 287, 384, 421, 669
BspCNI CTCAG 4 cut(s) 35, 81, 637, 870
BspLI GGNNCC 2 cut(s) 552, 748
BspTI CTTAAG 2 cut(s) 929, 940
BspTNI GGTCTC 1 cut(s) 860
BsrFI RCCGGY 2 cut(s) 253, 875
BsrI ACTGG 1 cut(s) 164
BssAI RCCGGY 2 cut(s) 253, 875
BssECI CCNNGG 3 cut(s) 318, 724, 850
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 850
Bst2BI CACGAG 1 cut(s) 195
Bst4CI ACNGT 2 cut(s) 526, 642
Bst6I CTCTTC 2 cut(s) 575, 893
BstAFI CTTAAG 2 cut(s) 929, 940
BstC8I GCNNGC 2 cut(s) 419, 667
BstDEI CTNAG 4 cut(s) 22, 68, 624, 857
BstF5I GGATG 2 cut(s) 328, 915
BstMAI GTCTC 1 cut(s) 860
BstMCI CGRYCG 1 cut(s) 254
BstSCI CCNGG 3 cut(s) 317, 723, 724
BstSFI CTRYAG 1 cut(s) 672
BstSLI GKGCMC 1 cut(s) 561
BstV1I GCAGC 8 cut(s) 298, 322, 331, 334, 337, 340, 344, 604
BstXI CCANNNNNNTGG 1 cut(s) 692
BstZI CGGCCG 1 cut(s) 251
BsuRI GGCC 5 cut(s) 253, 287, 384, 421, 669
BtsCI GGATG 2 cut(s) 328, 915
BtsI GCAGTG 2 cut(s) 339, 795
BtsIMutI CAGTG 2 cut(s) 339, 795
Cac8I GCNNGC 2 cut(s) 419, 667
Cfr10I RCCGGY 2 cut(s) 253, 875
Cfr13I GGNCC 2 cut(s) 285, 668
Cfr9I CCCGGG 1 cut(s) 724
CspAI ACCGGT 1 cut(s) 875
CviAII CATG 3 cut(s) 547, 738, 907
DdeI CTNAG 4 cut(s) 22, 68, 624, 857
EaeI YGGCCR 1 cut(s) 251
EagI CGGCCG 1 cut(s) 251
Eam1104I CTCTTC 2 cut(s) 575, 893
EarI CTCTTC 2 cut(s) 575, 893
EclXI CGGCCG 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 850
Eco31I GGTCTC 1 cut(s) 860
Eco52I CGGCCG 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 850
ErhI CCWWGG 1 cut(s) 850
FaeI CATG 3 cut(s) 550, 741, 910
FaiI YATR 8 cut(s) 143, 518, 548, 569, 739, 773, 864, 908
FalI AAGNNNNNCTT 2 cut(s) 923, 955
FaqI GGGAC 1 cut(s) 155
FatI CATG 3 cut(s) 546, 737, 906
Fnu4HI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 354, 618
FokI GGATG 2 cut(s) 335, 902
Fsp4HI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 354, 618
FspBI CTAG 2 cut(s) 530, 830
GluI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 354, 618
GsaI CCCAGC 1 cut(s) 825
HaeIII GGCC 5 cut(s) 253, 287, 384, 421, 669
HapII CCGG 5 cut(s) 254, 318, 458, 725, 876
Hin1II CATG 3 cut(s) 550, 741, 910
HindIII AAGCTT 2 cut(s) 146, 183
HinfI GANTC 4 cut(s) 49, 138, 359, 925
HpaII CCGG 5 cut(s) 254, 318, 458, 725, 876
HphI GGTGA 3 cut(s) 69, 472, 856
Hpy166II GTNNAC 1 cut(s) 847
Hpy188I TCNGA 5 cut(s) 71, 180, 699, 860, 924
Hpy188III TCNNGA 1 cut(s) 767
Hpy8I GTNNAC 1 cut(s) 847
HpyAV CCTTC 3 cut(s) 571, 643, 929
HpyCH4III ACNGT 2 cut(s) 526, 642
HpyCH4IV ACGT 1 cut(s) 902
HpyCH4V TGCA 5 cut(s) 332, 443, 502, 590, 620
HpyF3I CTNAG 4 cut(s) 22, 68, 624, 857
HpySE526I ACGT 1 cut(s) 902
Hsp92II CATG 3 cut(s) 550, 741, 910
LmnI GCTCC 3 cut(s) 734, 746, 912
Lsp1109I GCAGC 8 cut(s) 298, 322, 331, 334, 337, 340, 344, 604
LweI GCATC 6 cut(s) 35, 313, 599, 629, 690, 924
MaeI CTAG 2 cut(s) 530, 830
MaeII ACGT 1 cut(s) 902
MaeIII GTNAC 2 cut(s) 7, 871
MboII GAAGA 5 cut(s) 195, 233, 400, 592, 880
MhlI GDGCHC 1 cut(s) 561
MluCI AATT 5 cut(s) 44, 237, 260, 799, 835
MlyI GAGTC 1 cut(s) 132
MseI TTAA 3 cut(s) 35, 930, 941
MspA1I CMGCKG 1 cut(s) 335
MspCI CTTAAG 2 cut(s) 929, 940
MspI CCGG 5 cut(s) 254, 318, 458, 725, 876
MspR9I CCNGG 3 cut(s) 319, 725, 726
NciI CCSGG 3 cut(s) 319, 725, 726
NlaIII CATG 3 cut(s) 550, 741, 910
NlaIV GGNNCC 2 cut(s) 552, 748
PfeI GAWTC 3 cut(s) 49, 359, 925
PinAI ACCGGT 1 cut(s) 875
PkrI GCNGC 8 cut(s) 313, 334, 337, 346, 349, 352, 355, 619
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 821
PspN4I GGNNCC 2 cut(s) 552, 748
PspPI GGNCC 2 cut(s) 285, 668
PvuII CAGCTG 1 cut(s) 335
SaqAI TTAA 3 cut(s) 35, 930, 941
SatI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 354, 618
Sau96I GGNCC 2 cut(s) 285, 668
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 3 cut(s) 319, 725, 726
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 6 cut(s) 35, 313, 599, 629, 690, 924
SfcI CTRYAG 1 cut(s) 672
SmaI CCCGGG 1 cut(s) 726
SmlI CTYRAG 3 cut(s) 506, 929, 940
SmoI CTYRAG 3 cut(s) 506, 929, 940
Sse9I AATT 5 cut(s) 44, 237, 260, 799, 835
SsiI CCGC 1 cut(s) 795
SspMI CTAG 2 cut(s) 530, 830
StyD4I CCNGG 3 cut(s) 317, 723, 724
StyI CCWWGG 1 cut(s) 850
TaaI ACNGT 2 cut(s) 526, 642
TaiI ACGT 1 cut(s) 905
TaqI TCGA 1 cut(s) 362
TasI AATT 5 cut(s) 44, 237, 260, 799, 835
TfiI GAWTC 3 cut(s) 49, 359, 925
Tru1I TTAA 3 cut(s) 35, 930, 941
Tru9I TTAA 3 cut(s) 35, 930, 941
TscAI CASTG 2 cut(s) 346, 795
TseI GCWGC 8 cut(s) 311, 332, 335, 344, 347, 350, 353, 617
TspDTI ATGAA 2 cut(s) 553, 895
TspMI CCCGGG 1 cut(s) 724
TspRI CASTG 2 cut(s) 346, 795
Vha464I CTTAAG 2 cut(s) 929, 940
XapI RAATTY 3 cut(s) 260, 799, 835
XmaI CCCGGG 1 cut(s) 724
XspI CTAG 2 cut(s) 530, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.