Rw4G032640

Putative small multi-drug export protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011202
Physical Location & Seq
Reverse (-)
59743588 .. 59746850
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032640.1

Sequence Viewer

Length: 981 bp
ATGGCTGTAACTCTATCAGCCTCAGCATCTCCATTAACATCAAAATTGATTCTTGGCAAAACCCTCCTCAGATTTTCACCAAAACACTCCCACCCTTTCACTCCCCATACCGAACAACCCCCATTCAATCCAAATGGGCTCATAAAGCTTCAATCTTTACGCCCAGTAGTCCCTATTCTGAAAGCTTCTCCTCCACGAGCTTCTTCAAATGAGTTTGAGGAAGATGAGGTCTTGTCAATTTTCCAAGAACGGCCGGTCAAATTTGGGCTTTGGGTGTTGCTTTGGGCCTCTGTTTCACTTGCTTGGTTTGCTGCTTCCGGGGATGCCAATGCAGCTGCCACTGCTGCTGCTGCTTCTGCTGATTCGATTAGAGCTTCCTCTTTCGGCCTGAAGATTGCGAATGGGCTTCGGGGTTTGGGCTGGCCTGATGAGGCTGTGGTGTTTGCATTGGCTACGCTTCCGGTGATTGAGCTTCGTGGGGCTATTCCTGTTGGTTATTGGTTGCAACTCAAGCCTGTTATGCTAACCGTTCTAGCTGTTCTTGGGAACATGGTTCCTGTGCCCTTCATCATACTTTACTTGAAGAGATTTGCATCTTTCCTTGCTGGAAAGAATAAAGCTGCATCTCAGTTCCTTGACTTATTGTTTGAGAAGGCTAAAAAGAAAGCTGGCCCTGTAGAGGAGTTCCAATGGCTTGGTCTGATGCTGTTTGTGGCTGTGCCTTTCCCGGGAACAGGAGCATGGACAGGAGCCATTGTAGCTTCTATTCTTGATATGCCATTTTGGTCAGCAGTGTCCGCAAATTTCTTTGGTGTTGTGTTGGCTGGGCTTCTAGTGAATTTGCTGGTGAACCTTGGTCTCAGATATGCTATTGTTACTGGTATTATCAGTAAGTCAAAGGTAGAAGGTGGCCACCCCTTATCAGACGCCATCGACGAAAATGAACTACCTGACAAGAAGGTGTGGCAGCCACGTGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sm_multidrug_ex

Protein Analysis

326

Amino Acids

35.02

Weight (kDa)

9.47

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sm_multidrug_ex PF06695 157 - 277 4.5e-39 Putative small multi-drug export protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02590
fragaria_vesca FvH4_4g32070
malus_domestica MD13G1079600.v1.1 MD16G1079500.v1.1
prunus_persica Prupe.1G267200_v2.0.a1
pyrus_communis pycom13g06930 pycom16g06870
rosa_chinensis RchiOBHm_Chr4g0440761
rosa_laevigata RLG00000006156
rosa_multiflora Rmu_co8415195.1_g000001 Rmu_sc0000424.1_g000005 Rmu_sc0022158.1_g000003
rosa_roxburghii Rroxscaffold_5G00381520
rosa_rugosa Rorug04G0327700
rosa_samantha Rh4AG379500 Rh4BG392400 Rh4CG407100 Rh4DG386200
rosa_wichuraiana Rw4G032640 Rw4G032670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 798
AcoI YGGCCR 2 cut(s) 251, 910
AcsI RAATTY 3 cut(s) 260, 802, 838
AcuI CTGAAG 1 cut(s) 410
AcvI CACGTG 1 cut(s) 974
AcyI GRCGYC 1 cut(s) 927
AfiI CCNNNNNNNGG 3 cut(s) 679, 728, 734
AgsI TTSAA 4 cut(s) 127, 152, 207, 583
Alw26I GTCTC 1 cut(s) 863
Ama87I CYCGRG 1 cut(s) 727
AoxI GGCC 6 cut(s) 251, 285, 385, 422, 670, 910
ApeKI GCWGC 8 cut(s) 311, 332, 335, 344, 347, 350, 620, 967
ApoI RAATTY 3 cut(s) 260, 802, 838
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
AspS9I GGNCC 2 cut(s) 285, 671
AsuC2I CCSGG 3 cut(s) 319, 728, 729
AsuHPI GGTGA 3 cut(s) 69, 475, 859
AvaI CYCGRG 1 cut(s) 727
BaeGI GKGCMC 1 cut(s) 564
BalI TGGCCA 1 cut(s) 912
BanII GRGCYC 1 cut(s) 141
BauI CACGAG 1 cut(s) 195
BbrPI CACGTG 1 cut(s) 974
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 7 cut(s) 298, 322, 331, 334, 337, 344, 607
BccI CCATC 1 cut(s) 938
BceAI ACGGC 1 cut(s) 266
BcnI CCSGG 3 cut(s) 319, 728, 729
BcoDI GTCTC 1 cut(s) 863
BfaI CTAG 2 cut(s) 533, 833
BfmI CTRYAG 1 cut(s) 675
BisI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 621, 968
BlsI GCNGC 8 cut(s) 313, 334, 337, 346, 349, 352, 622, 969
Bme1390I CCNGG 3 cut(s) 319, 728, 729
BmeT110I CYCGRG 1 cut(s) 727
BmgT120I GGNCC 2 cut(s) 285, 671
BmiI GGNNCC 2 cut(s) 555, 751
BmrFI CCNGG 3 cut(s) 319, 728, 729
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 5 cut(s) 35, 313, 602, 632, 693
BmuI ACTGGG 1 cut(s) 158
Bpu10I CCTNAGC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 3 cut(s) 319, 728, 729
BsaAI YACGTR 1 cut(s) 974
BsaBI GATNNNNATC 1 cut(s) 592
BsaHI GRCGYC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 863
BsaJI CCNNGG 3 cut(s) 318, 727, 853
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 741, 771
Bsc4I CCNNNNNNNGG 3 cut(s) 679, 728, 734
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 2 cut(s) 164, 883
Bse8I GATNNNNATC 1 cut(s) 592
BseDI CCNNGG 3 cut(s) 318, 727, 853
BseGI GGATG 1 cut(s) 328
BseJI GATNNNNATC 1 cut(s) 592
BseLI CCNNNNNNNGG 3 cut(s) 679, 728, 734
BseMII CTCAG 4 cut(s) 36, 82, 641, 874
BseNI ACTGG 2 cut(s) 164, 883
BseRI GAGGAG 3 cut(s) 56, 180, 695
BseSI GKGCMC 1 cut(s) 564
BseX3I CGGCCG 1 cut(s) 251
BseXI GCAGC 7 cut(s) 298, 322, 331, 334, 337, 344, 607
BseYI CCCAGC 1 cut(s) 824
Bsh1285I CGRYCG 1 cut(s) 254
BshFI GGCC 6 cut(s) 253, 287, 387, 424, 672, 912
BsiEI CGRYCG 1 cut(s) 254
BsiHKCI CYCGRG 1 cut(s) 727
BsiSI CCGG 4 cut(s) 254, 318, 461, 728
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 3 cut(s) 679, 728, 734
BsmAI GTCTC 1 cut(s) 863
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 6 cut(s) 253, 287, 387, 424, 672, 912
Bso31I GGTCTC 1 cut(s) 863
BsoBI CYCGRG 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 141, 564
BspACI CCGC 1 cut(s) 798
BspANI GGCC 6 cut(s) 253, 287, 387, 424, 672, 912
BspCNI CTCAG 4 cut(s) 35, 81, 640, 873
BspLI GGNNCC 2 cut(s) 555, 751
BspTNI GGTCTC 1 cut(s) 863
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 2 cut(s) 164, 883
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 3 cut(s) 318, 727, 853
BssNI GRCGYC 1 cut(s) 927
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 853
Bst2BI CACGAG 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 529
Bst6I CTCTTC 1 cut(s) 578
BstACI GRCGYC 1 cut(s) 927
BstBAI YACGTR 1 cut(s) 974
BstC8I GCNNGC 2 cut(s) 422, 670
BstDEI CTNAG 4 cut(s) 22, 68, 627, 860
BstF5I GGATG 1 cut(s) 328
BstMAI GTCTC 1 cut(s) 863
BstMCI CGRYCG 1 cut(s) 254
BstSCI CCNGG 3 cut(s) 317, 726, 727
BstSFI CTRYAG 1 cut(s) 675
BstSLI GKGCMC 1 cut(s) 564
BstV1I GCAGC 7 cut(s) 298, 322, 331, 334, 337, 344, 607
BstXI CCANNNNNNTGG 1 cut(s) 695
BstZI CGGCCG 1 cut(s) 251
BsuRI GGCC 6 cut(s) 253, 287, 387, 424, 672, 912
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 2 cut(s) 339, 798
BtsIMutI CAGTG 2 cut(s) 339, 798
Cac8I GCNNGC 2 cut(s) 422, 670
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 285, 671
Cfr9I CCCGGG 1 cut(s) 727
CseI GACGC 1 cut(s) 935
CviAII CATG 2 cut(s) 550, 741
DdeI CTNAG 4 cut(s) 22, 68, 627, 860
EaeI YGGCCR 2 cut(s) 251, 910
EagI CGGCCG 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 578
EarI CTCTTC 1 cut(s) 578
EclXI CGGCCG 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 863
Eco52I CGGCCG 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 410
Eco72I CACGTG 1 cut(s) 974
Eco88I CYCGRG 1 cut(s) 727
EcoT14I CCWWGG 1 cut(s) 853
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 853
FaeI CATG 2 cut(s) 553, 744
FaiI YATR 8 cut(s) 108, 143, 521, 551, 572, 742, 776, 867
FaqI GGGAC 1 cut(s) 155
FatI CATG 2 cut(s) 549, 740
Fnu4HI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 621, 968
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 621, 968
FspBI CTAG 2 cut(s) 533, 833
GluI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 621, 968
GsaI CCCAGC 1 cut(s) 828
HaeIII GGCC 6 cut(s) 253, 287, 387, 424, 672, 912
HapII CCGG 4 cut(s) 254, 318, 461, 728
HgaI GACGC 1 cut(s) 935
Hin1I GRCGYC 1 cut(s) 927
Hin1II CATG 2 cut(s) 553, 744
HindIII AAGCTT 2 cut(s) 146, 183
HinfI GANTC 2 cut(s) 49, 362
HpaII CCGG 4 cut(s) 254, 318, 461, 728
HphI GGTGA 3 cut(s) 69, 475, 859
Hpy166II GTNNAC 1 cut(s) 850
Hpy188I TCNGA 5 cut(s) 71, 180, 702, 863, 925
Hpy188III TCNNGA 1 cut(s) 770
Hpy8I GTNNAC 1 cut(s) 850
Hpy99I CGWCG 1 cut(s) 938
HpyAV CCTTC 4 cut(s) 574, 646, 899, 952
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 1 cut(s) 973
HpyCH4V TGCA 5 cut(s) 332, 446, 505, 593, 623
HpyF3I CTNAG 4 cut(s) 22, 68, 627, 860
HpySE526I ACGT 1 cut(s) 973
Hsp92I GRCGYC 1 cut(s) 927
Hsp92II CATG 2 cut(s) 553, 744
LmnI GCTCC 2 cut(s) 737, 749
Lsp1109I GCAGC 7 cut(s) 298, 322, 331, 334, 337, 344, 607
LweI GCATC 5 cut(s) 35, 313, 602, 632, 693
MaeI CTAG 2 cut(s) 533, 833
MaeII ACGT 1 cut(s) 973
MaeIII GTNAC 2 cut(s) 7, 874
MboII GAAGA 4 cut(s) 195, 233, 403, 595
MhlI GDGCHC 2 cut(s) 141, 564
MlsI TGGCCA 1 cut(s) 912
MluCI AATT 5 cut(s) 44, 237, 260, 802, 838
MluNI TGGCCA 1 cut(s) 912
Mox20I TGGCCA 1 cut(s) 912
MscI TGGCCA 1 cut(s) 912
MseI TTAA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 912
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 4 cut(s) 254, 318, 461, 728
MspR9I CCNGG 3 cut(s) 319, 728, 729
NciI CCSGG 3 cut(s) 319, 728, 729
NlaIII CATG 2 cut(s) 553, 744
NlaIV GGNNCC 2 cut(s) 555, 751
PcsI WCGNNNNNNNCGW 1 cut(s) 933
PfeI GAWTC 2 cut(s) 49, 362
PkrI GCNGC 8 cut(s) 313, 334, 337, 346, 349, 352, 622, 969
PmaCI CACGTG 1 cut(s) 974
PmlI CACGTG 1 cut(s) 974
Ppu21I YACGTR 1 cut(s) 974
PspCI CACGTG 1 cut(s) 974
PspFI CCCAGC 1 cut(s) 824
PspN4I GGNNCC 2 cut(s) 555, 751
PspPI GGNCC 2 cut(s) 285, 671
PvuII CAGCTG 1 cut(s) 335
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 8 cut(s) 312, 333, 336, 345, 348, 351, 621, 968
Sau96I GGNCC 2 cut(s) 285, 671
ScrFI CCNGG 3 cut(s) 319, 728, 729
SduI GDGCHC 2 cut(s) 141, 564
SfaNI GCATC 5 cut(s) 35, 313, 602, 632, 693
SfcI CTRYAG 1 cut(s) 675
SmaI CCCGGG 1 cut(s) 729
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 5 cut(s) 44, 237, 260, 802, 838
SsiI CCGC 1 cut(s) 798
SspMI CTAG 2 cut(s) 533, 833
StyD4I CCNGG 3 cut(s) 317, 726, 727
StyI CCWWGG 1 cut(s) 853
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 1 cut(s) 976
TaqI TCGA 2 cut(s) 365, 933
TasI AATT 5 cut(s) 44, 237, 260, 802, 838
TfiI GAWTC 2 cut(s) 49, 362
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 2 cut(s) 346, 798
TseI GCWGC 8 cut(s) 311, 332, 335, 344, 347, 350, 620, 967
TspDTI ATGAA 2 cut(s) 556, 957
TspMI CCCGGG 1 cut(s) 727
TspRI CASTG 2 cut(s) 346, 798
XapI RAATTY 3 cut(s) 260, 802, 838
XmaI CCCGGG 1 cut(s) 727
XspI CTAG 2 cut(s) 533, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.