FvH4_4g32860

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010303
Physical Location & Seq
Reverse (-)
31318164 .. 31320765
2602 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32860.t2

Sequence Viewer

Length: 654 bp
ATGGGATCAGAAGGGCCAAAGAGCGTGGTGATTAATGTGAGTGGGTTCAAGAAGTTTCAAGGGGTGGCTGAGAATCCAACTGAGGTTATTGTCAACAATCTGAGTAGCTATGTGGAGAAGCGAGGGTTGTCTTCTGGTCTGACACTGGGGAGCTGCACCATTCTCGAGACTGCTGGAGATGGTGCTAGTGCTGCTCTCTACAAGGCCATGGAGTCCGGGATTGAGAGAATGGAGTCTAATGCCAATGATCAAGTTGTATGGCTTCACTTGGGGGTGAACAGTGGGGCTATGAAATTTGCTATTGAGCGGCAAGCTGTAAATGAAGCCACCTTTCGCTGTCCGGATGAGTTTGGATGGCAACCTCAGCAATTCCCAATAATTGAAGCAGATGGGGTAATTTCTCGAGCTAGAGAGACATCTTGTTCGATTGATGCCATCCTTAAGATCTTGAAGAAAAAGGGATTCGACGCGGCCATATCAGATGATGCAGGACGCTTTGTGTGCAATTACGTCTACTACCATTCTCTCCGGTTTGCAGAAGAAAAGGGTCACAAGTCTCTGTTTGTCCATGTTCCCCTCTTTACAAGAATCGATGAAGAAACTCAGATGCGGTTTGTGGCCTCTCTTCTGGATGCCATTGCCGCAACATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

218

Amino Acids

23.71

Weight (kDa)

5.45

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C15 PF01470 12 - 194 3.1e-18 Pyroglutamyl peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 513
AccII CGCG 1 cut(s) 470
AccIII TCCGGA 1 cut(s) 340
AciI CCGC 4 cut(s) 307, 470, 610, 642
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 293
AflII CTTAAG 1 cut(s) 440
AgsI TTSAA 4 cut(s) 49, 59, 383, 451
AluBI AGCT 4 cut(s) 108, 153, 314, 407
AluI AGCT 4 cut(s) 108, 153, 314, 407
Alw26I GTCTC 3 cut(s) 161, 407, 561
AlwI GGATC 1 cut(s) 13
Ama87I CYCGRG 2 cut(s) 164, 402
Aor13HI TCCGGA 1 cut(s) 340
AoxI GGCC 4 cut(s) 14, 204, 471, 618
ApeKI GCWGC 2 cut(s) 153, 191
ApoI RAATTY 1 cut(s) 293
ArsI GACNNNNNNTTYG 2 cut(s) 406, 438
AseI ATTAAT 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 217
AsuHPI GGTGA 2 cut(s) 40, 286
AvaI CYCGRG 2 cut(s) 164, 402
BbsI GAAGAC 1 cut(s) 123
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 140, 178
BccI CCATC 4 cut(s) 173, 348, 383, 443
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 1 cut(s) 217
BcoDI GTCTC 3 cut(s) 161, 407, 561
BfaI CTAG 2 cut(s) 186, 408
BfrI CTTAAG 1 cut(s) 440
BglII AGATCT 1 cut(s) 444
BisI GCNGC 5 cut(s) 154, 192, 308, 471, 642
BlsI GCNGC 5 cut(s) 155, 193, 309, 472, 643
Bme1390I CCNGG 1 cut(s) 217
BmeT110I CYCGRG 2 cut(s) 164, 402
BmgT120I GGNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 217
BmrI ACTGGG 1 cut(s) 155
BmsI GCATC 4 cut(s) 421, 475, 597, 622
BmuI ACTGGG 1 cut(s) 155
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 217
Bsa29I ATCGAT 1 cut(s) 591
BsaJI CCNNGG 1 cut(s) 207
BsaWI WCCGGW 2 cut(s) 340, 528
Bse1I ACTGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 636
BseAI TCCGGA 1 cut(s) 340
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 1 cut(s) 207
BseGI GGATG 4 cut(s) 349, 359, 435, 637
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 5 cut(s) 60, 72, 92, 377, 617
BseNI ACTGG 1 cut(s) 150
BseXI GCAGC 2 cut(s) 140, 178
BsgI GTGCAG 1 cut(s) 139
Bsh1236I CGCG 1 cut(s) 470
BshFI GGCC 4 cut(s) 16, 206, 473, 620
BshVI ATCGAT 1 cut(s) 591
BsiHKCI CYCGRG 2 cut(s) 164, 402
BsiSI CCGG 3 cut(s) 216, 341, 529
BsmAI GTCTC 3 cut(s) 161, 407, 561
BsnI GGCC 4 cut(s) 16, 206, 473, 620
BsoBI CYCGRG 2 cut(s) 164, 402
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 3 cut(s) 5, 247, 444
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 4 cut(s) 307, 470, 610, 642
BspANI GGCC 4 cut(s) 16, 206, 473, 620
BspCNI CTCAG 5 cut(s) 61, 73, 93, 376, 616
BspDI ATCGAT 1 cut(s) 591
BspEI TCCGGA 1 cut(s) 340
BspFNI CGCG 1 cut(s) 470
BspPI GGATC 1 cut(s) 13
BspTI CTTAAG 1 cut(s) 440
BsrBI CCGCTC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 3 cut(s) 5, 247, 444
BssT1I CCWWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 281
Bst6I CTCTTC 1 cut(s) 630
BstAFI CTTAAG 1 cut(s) 440
BstC8I GCNNGC 1 cut(s) 312
BstDEI CTNAG 5 cut(s) 69, 81, 101, 363, 603
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 4 cut(s) 349, 359, 435, 637
BstFNI CGCG 1 cut(s) 470
BstKTI GATC 3 cut(s) 8, 250, 447
BstMAI GTCTC 3 cut(s) 161, 407, 561
BstMBI GATC 3 cut(s) 5, 247, 444
BstMWI GCNNNNNNNGC 4 cut(s) 191, 364, 501, 641
BstNSI RCATGY 1 cut(s) 651
BstSCI CCNGG 1 cut(s) 215
BstUI CGCG 1 cut(s) 470
BstV1I GCAGC 2 cut(s) 140, 178
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 444
BstYI RGATCY 1 cut(s) 444
Bsu15I ATCGAT 1 cut(s) 591
BsuRI GGCC 4 cut(s) 16, 206, 473, 620
BsuTUI ATCGAT 1 cut(s) 591
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 4 cut(s) 349, 359, 435, 637
BtsIMutI CAGTG 2 cut(s) 143, 286
Cac8I GCNNGC 1 cut(s) 312
Cfr13I GGNCC 1 cut(s) 14
ClaI ATCGAT 1 cut(s) 591
CseI GACGC 2 cut(s) 476, 501
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 3 cut(s) 208, 569, 648
DdeI CTNAG 5 cut(s) 69, 81, 101, 363, 603
DpnI GATC 3 cut(s) 7, 249, 446
DpnII GATC 3 cut(s) 5, 247, 444
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
Eco130I CCWWGG 1 cut(s) 207
Eco88I CYCGRG 2 cut(s) 164, 402
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 3 cut(s) 211, 572, 651
FaiI YATR 7 cut(s) 111, 209, 259, 290, 476, 570, 649
FatI CATG 3 cut(s) 207, 568, 647
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 5 cut(s) 154, 192, 308, 471, 642
FokI GGATG 4 cut(s) 356, 366, 422, 644
Fsp4HI GCNGC 5 cut(s) 154, 192, 308, 471, 642
FspBI CTAG 2 cut(s) 186, 408
GluI GCNGC 5 cut(s) 154, 192, 308, 471, 642
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 4 cut(s) 16, 206, 473, 620
HapII CCGG 3 cut(s) 216, 341, 529
HgaI GACGC 2 cut(s) 476, 501
Hin1II CATG 3 cut(s) 211, 572, 651
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 5 cut(s) 73, 212, 233, 462, 588
HpaII CCGG 3 cut(s) 216, 341, 529
HphI GGTGA 2 cut(s) 40, 286
Hpy166II GTNNAC 3 cut(s) 94, 277, 514
Hpy188I TCNGA 5 cut(s) 10, 102, 141, 481, 606
Hpy188III TCNNGA 7 cut(s) 49, 164, 166, 341, 402, 448, 629
Hpy8I GTNNAC 3 cut(s) 94, 277, 514
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 4 cut(s) 156, 488, 504, 536
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 364, 501, 641
HpyF3I CTNAG 5 cut(s) 69, 81, 101, 363, 603
HpySE526I ACGT 1 cut(s) 510
Hsp92II CATG 3 cut(s) 211, 572, 651
Kpn2I TCCGGA 1 cut(s) 340
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 3 cut(s) 5, 247, 444
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 8 cut(s) 120, 131, 159, 229, 354, 474, 542, 614
Lsp1109I GCAGC 2 cut(s) 140, 178
LweI GCATC 4 cut(s) 421, 475, 597, 622
MaeI CTAG 2 cut(s) 186, 408
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 1 cut(s) 548
MalI GATC 3 cut(s) 7, 249, 446
MbiI CCGCTC 1 cut(s) 307
MboI GATC 3 cut(s) 5, 247, 444
MboII GAAGA 5 cut(s) 123, 463, 551, 608, 617
MflI RGATCY 1 cut(s) 444
MluCI AATT 5 cut(s) 293, 368, 378, 396, 505
MlyI GAGTC 2 cut(s) 221, 242
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 5 cut(s) 76, 116, 372, 587, 631
MroI TCCGGA 1 cut(s) 340
MseI TTAA 2 cut(s) 33, 441
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 3 cut(s) 216, 341, 529
MspR9I CCNGG 1 cut(s) 217
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 4 cut(s) 191, 364, 501, 641
NciI CCSGG 1 cut(s) 217
NcoI CCATGG 1 cut(s) 207
NdeII GATC 3 cut(s) 5, 247, 444
NlaIII CATG 3 cut(s) 211, 572, 651
NmuCI GTSAC 1 cut(s) 548
NspI RCATGY 1 cut(s) 651
PaeR7I CTCGAG 2 cut(s) 164, 402
PfeI GAWTC 3 cut(s) 73, 462, 588
PfoI TCCNGGA 1 cut(s) 215
PkrI GCNGC 5 cut(s) 155, 193, 309, 472, 643
PleI GAGTC 2 cut(s) 220, 241
PpsI GAGTC 2 cut(s) 220, 241
PshBI ATTAAT 1 cut(s) 33
PspPI GGNCC 1 cut(s) 14
PsuI RGATCY 1 cut(s) 444
SaqAI TTAA 2 cut(s) 33, 441
SatI GCNGC 5 cut(s) 154, 192, 308, 471, 642
Sau3AI GATC 3 cut(s) 5, 247, 444
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 2 cut(s) 221, 242
ScrFI CCNGG 1 cut(s) 217
SetI ASST 8 cut(s) 87, 110, 155, 316, 332, 364, 409, 513
SfaNI GCATC 4 cut(s) 421, 475, 597, 622
Sfr274I CTCGAG 2 cut(s) 164, 402
SlaI CTCGAG 2 cut(s) 164, 402
SmlI CTYRAG 3 cut(s) 164, 402, 440
SmoI CTYRAG 3 cut(s) 164, 402, 440
Sse9I AATT 5 cut(s) 293, 368, 378, 396, 505
SsiI CCGC 4 cut(s) 307, 470, 610, 642
SspMI CTAG 2 cut(s) 186, 408
StyD4I CCNGG 1 cut(s) 215
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 1 cut(s) 513
TaqI TCGA 5 cut(s) 165, 403, 425, 465, 591
TasI AATT 5 cut(s) 293, 368, 378, 396, 505
TauI GCSGC 3 cut(s) 310, 473, 644
TfiI GAWTC 3 cut(s) 73, 462, 588
Tru1I TTAA 2 cut(s) 33, 441
Tru9I TTAA 2 cut(s) 33, 441
TscAI CASTG 2 cut(s) 150, 286
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 2 cut(s) 153, 191
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 3 cut(s) 305, 336, 609
TspRI CASTG 2 cut(s) 150, 286
Vha464I CTTAAG 1 cut(s) 440
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 1 cut(s) 293
XceI RCATGY 1 cut(s) 651
XhoI CTCGAG 2 cut(s) 164, 402
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 2 cut(s) 186, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.