Rw4G033530

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010303
Physical Location & Seq
Reverse (-)
60682599 .. 60685467
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033530.1

Sequence Viewer

Length: 654 bp
ATGGGATCAGAAGGGCCAAAGAGTGTGGTGATTAATGTGACTGGTTTCAAGAAGTTTCAAGGGGTGGCTGAGAATCCAACAGAGATTATAGTGAACAATCTAAAGACCTATGTGGAGAAGAGAGGGTTGCCTTCTGGTCTGACACTTGGTAGCTGCACCATTCTTGAGACTGCTGGAGATGGTGCCAGTCCAGCTCTCTACAAGGCCATGGAGTCGGGGATTTCGAAAACGGACTCTAACACCAATGATCAAGTTGTCTGGCTTCACTTGGGGGTGAATAGTGGGGCTATGAAATTTGCTATTGAGCGGCAAGCTGTAAATGAGGCCACCTTTCGGTGTCCGGATGAGTTTGGATGGCAACCTCAGCAATTTCCAATAATTGAAGCAGATGGGGAAATTTCTCGAGCAAGAGAGACTTCTTGCTCAATTGATGCAATCCTTAAGATCTTGAAGAAGAAGGGATTCGATGTGGCGGTATCAGATGATGCGGGACGCTTTGTGTGCAATTACGTCTACTACCATTCTCTCCGGTTTGCAGAAGAAAAGGGTCACAAGTCTCTATTTGTCCATGTTCCTCTCTTTTCAAGAATTGATGAAGAAACTCAGATGCGCTTTGTGGCGTCTCTTCTGGAGGCTCTTGCTGCAACATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

217

Amino Acids

23.79

Weight (kDa)

5.64

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C15 PF01470 11 - 194 8.9e-19 Pyroglutamyl peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 513
AccIII TCCGGA 1 cut(s) 340
AciI CCGC 3 cut(s) 307, 473, 488
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 2 cut(s) 293, 396
AcyI GRCGYC 1 cut(s) 620
AfiI CCNNNNNNNGG 1 cut(s) 333
AflII CTTAAG 1 cut(s) 440
AgsI TTSAA 5 cut(s) 49, 59, 383, 451, 585
AluBI AGCT 3 cut(s) 153, 194, 314
AluI AGCT 3 cut(s) 153, 194, 314
Alw26I GTCTC 4 cut(s) 161, 407, 561, 627
AlwI GGATC 1 cut(s) 13
Ama87I CYCGRG 1 cut(s) 402
Aor13HI TCCGGA 1 cut(s) 340
AoxI GGCC 3 cut(s) 14, 204, 324
ApeKI GCWGC 2 cut(s) 153, 641
ApoI RAATTY 2 cut(s) 293, 396
AseI ATTAAT 1 cut(s) 33
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 1 cut(s) 612
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 40, 286
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 1 cut(s) 402
BanI GGYRCC 1 cut(s) 182
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 140, 628
BccI CCATC 3 cut(s) 173, 348, 383
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 4 cut(s) 161, 407, 561, 627
BfrI CTTAAG 1 cut(s) 440
BglII AGATCT 1 cut(s) 444
BisI GCNGC 3 cut(s) 154, 308, 642
BlsI GCNGC 3 cut(s) 155, 309, 643
BmeT110I CYCGRG 1 cut(s) 402
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 184
BmsI GCATC 3 cut(s) 421, 475, 597
BpmI CTGGAG 2 cut(s) 195, 650
Bpu10I CCTNAGC 1 cut(s) 363
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 620
BsaJI CCNNGG 1 cut(s) 207
BsaWI WCCGGW 2 cut(s) 340, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse1I ACTGG 2 cut(s) 46, 186
BseAI TCCGGA 1 cut(s) 340
BseDI CCNNGG 1 cut(s) 207
BseGI GGATG 2 cut(s) 349, 359
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMII CTCAG 3 cut(s) 60, 377, 617
BseNI ACTGG 2 cut(s) 46, 186
BseXI GCAGC 2 cut(s) 140, 628
BsgI GTGCAG 1 cut(s) 139
BshFI GGCC 3 cut(s) 16, 206, 326
BshNI GGYRCC 1 cut(s) 182
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 2 cut(s) 341, 529
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 4 cut(s) 161, 407, 561, 627
BsmBI CGTCTC 1 cut(s) 627
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 3 cut(s) 16, 206, 326
BsoBI CYCGRG 1 cut(s) 402
Bsp119I TTCGAA 1 cut(s) 224
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 3 cut(s) 5, 247, 444
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 3 cut(s) 307, 473, 488
BspANI GGCC 3 cut(s) 16, 206, 326
BspCNI CTCAG 3 cut(s) 61, 376, 616
BspEI TCCGGA 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 184
BspPI GGATC 1 cut(s) 13
BspT104I TTCGAA 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 182
BspTI CTTAAG 1 cut(s) 440
BsrBI CCGCTC 1 cut(s) 307
BsrI ACTGG 2 cut(s) 46, 186
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 3 cut(s) 5, 247, 444
BssNI GRCGYC 1 cut(s) 620
BssT1I CCWWGG 1 cut(s) 207
Bst6I CTCTTC 2 cut(s) 113, 630
BstACI GRCGYC 1 cut(s) 620
BstAFI CTTAAG 1 cut(s) 440
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 1 cut(s) 312
BstDEI CTNAG 3 cut(s) 69, 363, 603
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 349, 359
BstHHI GCGC 1 cut(s) 612
BstKTI GATC 3 cut(s) 8, 250, 447
BstMAI GTCTC 4 cut(s) 161, 407, 561, 627
BstMBI GATC 3 cut(s) 5, 247, 444
BstMWI GCNNNNNNNGC 4 cut(s) 191, 364, 501, 641
BstNSI RCATGY 1 cut(s) 651
BstV1I GCAGC 2 cut(s) 140, 628
BstX2I RGATCY 1 cut(s) 444
BstYI RGATCY 1 cut(s) 444
BsuRI GGCC 3 cut(s) 16, 206, 326
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 2 cut(s) 349, 359
Cac8I GCNNGC 1 cut(s) 312
CfoI GCGC 1 cut(s) 612
Cfr13I GGNCC 1 cut(s) 14
CseI GACGC 2 cut(s) 501, 609
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 3 cut(s) 208, 569, 648
DdeI CTNAG 3 cut(s) 69, 363, 603
DpnI GATC 3 cut(s) 7, 249, 446
DpnII GATC 3 cut(s) 5, 247, 444
Eam1104I CTCTTC 2 cut(s) 113, 630
EarI CTCTTC 2 cut(s) 113, 630
Eco130I CCWWGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
Esp3I CGTCTC 1 cut(s) 627
FaeI CATG 3 cut(s) 211, 572, 651
FaiI YATR 6 cut(s) 89, 111, 209, 290, 570, 649
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FaqI GGGAC 1 cut(s) 504
FatI CATG 3 cut(s) 207, 568, 647
FauI CCCGC 1 cut(s) 481
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 3 cut(s) 154, 308, 642
FokI GGATG 2 cut(s) 356, 366
Fsp4HI GCNGC 3 cut(s) 154, 308, 642
GlaI GCGC 1 cut(s) 611
GluI GCNGC 3 cut(s) 154, 308, 642
GsuI CTGGAG 2 cut(s) 195, 650
HaeIII GGCC 3 cut(s) 16, 206, 326
HapII CCGG 2 cut(s) 341, 529
HgaI GACGC 2 cut(s) 501, 609
HhaI GCGC 1 cut(s) 612
Hin1I GRCGYC 1 cut(s) 620
Hin1II CATG 3 cut(s) 211, 572, 651
Hin6I GCGC 1 cut(s) 610
HinP1I GCGC 1 cut(s) 610
HinfI GANTC 4 cut(s) 73, 212, 233, 462
HpaII CCGG 2 cut(s) 341, 529
HphI GGTGA 2 cut(s) 40, 286
Hpy166II GTNNAC 2 cut(s) 94, 514
Hpy188I TCNGA 4 cut(s) 10, 141, 481, 606
Hpy188III TCNNGA 7 cut(s) 49, 164, 341, 402, 448, 585, 629
Hpy8I GTNNAC 2 cut(s) 94, 514
HpyAV CCTTC 3 cut(s) 5, 141, 451
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 5 cut(s) 156, 434, 504, 536, 644
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 364, 501, 641
HpyF3I CTNAG 3 cut(s) 69, 363, 603
HpySE526I ACGT 1 cut(s) 510
Hsp92I GRCGYC 1 cut(s) 620
Hsp92II CATG 3 cut(s) 211, 572, 651
HspAI GCGC 1 cut(s) 610
Kpn2I TCCGGA 1 cut(s) 340
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 3 cut(s) 5, 247, 444
LpnPI CCDG 9 cut(s) 27, 120, 159, 199, 204, 244, 354, 542, 614
Lsp1109I GCAGC 2 cut(s) 140, 628
LweI GCATC 3 cut(s) 421, 475, 597
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 2 cut(s) 37, 548
MalI GATC 3 cut(s) 7, 249, 446
MbiI CCGCTC 1 cut(s) 307
MboI GATC 3 cut(s) 5, 247, 444
MboII GAAGA 6 cut(s) 130, 463, 466, 551, 608, 617
MfeI CAATTG 1 cut(s) 426
MflI RGATCY 1 cut(s) 444
MluCI AATT 7 cut(s) 293, 368, 378, 396, 426, 505, 588
MlyI GAGTC 2 cut(s) 221, 227
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 5 cut(s) 116, 316, 372, 585, 625
MroI TCCGGA 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 2 cut(s) 33, 441
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 2 cut(s) 341, 529
MunI CAATTG 1 cut(s) 426
MwoI GCNNNNNNNGC 4 cut(s) 191, 364, 501, 641
NcoI CCATGG 1 cut(s) 207
NdeII GATC 3 cut(s) 5, 247, 444
NlaIII CATG 3 cut(s) 211, 572, 651
NlaIV GGNNCC 1 cut(s) 184
NmuCI GTSAC 2 cut(s) 37, 548
NspI RCATGY 1 cut(s) 651
NspV TTCGAA 1 cut(s) 224
PaeR7I CTCGAG 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 2 cut(s) 73, 462
PkrI GCNGC 3 cut(s) 155, 309, 643
PleI GAGTC 2 cut(s) 220, 227
PpsI GAGTC 2 cut(s) 220, 227
PshBI ATTAAT 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 1 cut(s) 14
PsuI RGATCY 1 cut(s) 444
SaqAI TTAA 2 cut(s) 33, 441
SatI GCNGC 3 cut(s) 154, 308, 642
Sau3AI GATC 3 cut(s) 5, 247, 444
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 2 cut(s) 221, 227
SetI ASST 7 cut(s) 110, 155, 196, 316, 332, 364, 513
SfaNI GCATC 3 cut(s) 421, 475, 597
Sfr274I CTCGAG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 224
SlaI CTCGAG 1 cut(s) 402
SmlI CTYRAG 3 cut(s) 164, 402, 440
SmoI CTYRAG 3 cut(s) 164, 402, 440
Sse9I AATT 7 cut(s) 293, 368, 378, 396, 426, 505, 588
SsiI CCGC 3 cut(s) 307, 473, 488
StyI CCWWGG 1 cut(s) 207
TaiI ACGT 1 cut(s) 513
TaqI TCGA 3 cut(s) 224, 403, 465
TasI AATT 7 cut(s) 293, 368, 378, 396, 426, 505, 588
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 2 cut(s) 73, 462
Tru1I TTAA 2 cut(s) 33, 441
Tru9I TTAA 2 cut(s) 33, 441
TseFI GTSAC 2 cut(s) 37, 548
TseI GCWGC 2 cut(s) 153, 641
Tsp45I GTSAC 2 cut(s) 37, 548
TspDTI ATGAA 2 cut(s) 305, 609
TspGWI ACGGA 1 cut(s) 245
Vha464I CTTAAG 1 cut(s) 440
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 2 cut(s) 293, 396
XceI RCATGY 1 cut(s) 651
XhoI CTCGAG 1 cut(s) 402
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.