MD13G1094400.v1.1

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010303
Physical Location & Seq
Reverse (-)
6653419 .. 6656208
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1094400.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGGATCAGAAGGACCAAAGAGTGTGGTTGTTCATGTGAGCGGGTTCAAGAAGTTCCAGGGGGTTGCTGAGAATCCCACAGAGACTATAGTTAGCAATCTGAGGGGGTTTGTTGAGAAGAGAGCGTTGCCTGCTGGTCTTAAACTTGGCAGCTGTGATGTTCTTGAAACAGCAGGAGACGGTGCACGCGCTGCTCTTTACAAGGCCATGGAATCGGGGATTTCAGCAACCGATTCTAAGAGCCATGAACAAGTCGTATGGCTTCACTTGGGGGTAAATAGTGGGGCTGTAAAATTTGCTATTGAGCGGCAAGCAGTAAACGAAGCCACTTTCCGCTGTCCAGATGAGTTAGGATGGCAACCTCAGCAACAACCAATAGTCCCTGAGGATGGAGGAACTTCTCGAGTGAGAGAGACATGTTGCTCGACCGAGGCAATCCTTAAGATATTGAAGAAGAAAGGATATGATGTGGCAATATCAGATGATGCCGGGCGCTTTGTGTGCAATTATGTCTACTATCATTCCCTCCGTTTTGCAGAAGAAAGGTGCCACAAATCTTTATTCGTCCACGTTCCTCTCTTTTCCAGAATCAATGAAGAAACTCAGATGCGCTTTGTGGCCTCCCTTTTGGAGGCGATTGCAGCTACATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

218

Amino Acids

23.75

Weight (kDa)

6.6

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C15 PF01470 11 - 195 4.4e-18 Pyroglutamyl peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 546
AccBSI CCGCTC 2 cut(s) 42, 307
AccI GTMKAC 1 cut(s) 513
AccII CGCG 1 cut(s) 189
AciI CCGC 3 cut(s) 42, 307, 334
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 1 cut(s) 293
AfiI CCNNNNNNNGG 2 cut(s) 389, 631
AflII CTTAAG 1 cut(s) 440
AflIII ACRYGT 2 cut(s) 416, 647
AgsI TTSAA 3 cut(s) 49, 167, 451
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 2 cut(s) 153, 644
AluI AGCT 2 cut(s) 153, 644
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 3 cut(s) 77, 171, 407
Alw44I GTGCAC 1 cut(s) 183
AlwI GGATC 1 cut(s) 13
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 2 cut(s) 204, 618
ApaLI GTGCAC 1 cut(s) 183
ApeKI GCWGC 3 cut(s) 150, 191, 641
ApoI RAATTY 1 cut(s) 293
AspLEI GCGC 3 cut(s) 191, 495, 612
AspS9I GGNCC 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 402
AvaII GGWCC 1 cut(s) 14
AxyI CCTNAGG 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 187
BanI GGYRCC 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 187
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 162, 178
BccI CCATC 2 cut(s) 348, 383
BciT130I CCWGG 1 cut(s) 59
BcnI CCSGG 1 cut(s) 490
BcoDI GTCTC 3 cut(s) 77, 171, 407
BfmI CTRYAG 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 496
BfrI CTTAAG 1 cut(s) 440
BisI GCNGC 4 cut(s) 151, 192, 308, 642
BlsI GCNGC 4 cut(s) 152, 193, 309, 643
Bme1390I CCNGG 2 cut(s) 59, 490
Bme18I GGWCC 1 cut(s) 14
BmeT110I CYCGRG 1 cut(s) 402
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 548
BmrFI CCNGG 2 cut(s) 59, 490
BmsI GCATC 2 cut(s) 475, 597
Bpu10I CCTNAGC 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 490
BsaJI CCNNGG 3 cut(s) 58, 207, 429
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 631
Bse21I CCTNAGG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 3 cut(s) 58, 207, 429
BseGI GGATG 2 cut(s) 359, 394
BseLI CCNNNNNNNGG 2 cut(s) 389, 631
BseMII CTCAG 5 cut(s) 60, 92, 375, 377, 617
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 2 cut(s) 162, 178
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 429
BshFI GGCC 2 cut(s) 206, 620
BshNI GGYRCC 1 cut(s) 546
BsiEI CGRYCG 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 187
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 1 cut(s) 489
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 2 cut(s) 389, 631
BsmAI GTCTC 3 cut(s) 77, 171, 407
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 2 cut(s) 206, 620
BsoBI CYCGRG 1 cut(s) 402
Bsp1286I GDGCHC 1 cut(s) 187
Bsp143I GATC 1 cut(s) 5
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 3 cut(s) 42, 307, 334
BspANI GGCC 2 cut(s) 206, 620
BspCNI CTCAG 5 cut(s) 61, 93, 376, 376, 616
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 548
BspPI GGATC 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 546
BspTI CTTAAG 1 cut(s) 440
BsrBI CCGCTC 2 cut(s) 42, 307
BssECI CCNNGG 3 cut(s) 58, 207, 429
BssMI GATC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 1 cut(s) 113
BstAFI CTTAAG 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 191
BstC8I GCNNGC 3 cut(s) 132, 187, 312
BstDEI CTNAG 6 cut(s) 69, 101, 237, 363, 384, 603
BstDSI CCRYGG 1 cut(s) 207
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 2 cut(s) 359, 394
BstFNI CGCG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 3 cut(s) 191, 495, 612
BstKTI GATC 1 cut(s) 8
BstMAI GTCTC 3 cut(s) 77, 171, 407
BstMBI GATC 1 cut(s) 5
BstMCI CGRYCG 1 cut(s) 429
BstMWI GCNNNNNNNGC 5 cut(s) 131, 191, 364, 501, 641
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 2 cut(s) 420, 651
BstSCI CCNGG 2 cut(s) 57, 488
BstSFI CTRYAG 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 187
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 162, 178
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 2 cut(s) 206, 620
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 2 cut(s) 359, 394
Cac8I GCNNGC 3 cut(s) 132, 187, 312
CfoI GCGC 3 cut(s) 191, 495, 612
Cfr13I GGNCC 1 cut(s) 14
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 5 cut(s) 35, 208, 245, 417, 648
CviJI RGCY 8 cut(s) 153, 206, 243, 262, 287, 326, 620, 644
CviKI_1 RGCY 8 cut(s) 153, 206, 243, 262, 287, 326, 620, 644
DdeI CTNAG 6 cut(s) 69, 101, 237, 363, 384, 603
DpnI GATC 1 cut(s) 7
DpnII GATC 1 cut(s) 5
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 14
Eco81I CCTNAGG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 5 cut(s) 38, 211, 248, 420, 651
FaiI YATR 9 cut(s) 36, 89, 209, 246, 259, 418, 465, 510, 649
FaqI GGGAC 1 cut(s) 365
FatI CATG 5 cut(s) 34, 207, 244, 416, 647
FauI CCCGC 1 cut(s) 35
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 4 cut(s) 151, 192, 308, 642
FokI GGATG 2 cut(s) 366, 401
Fsp4HI GCNGC 4 cut(s) 151, 192, 308, 642
GlaI GCGC 3 cut(s) 190, 494, 611
GluI GCNGC 4 cut(s) 151, 192, 308, 642
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 2 cut(s) 206, 620
HapII CCGG 1 cut(s) 489
HhaI GCGC 3 cut(s) 191, 495, 612
Hin1II CATG 5 cut(s) 38, 211, 248, 420, 651
Hin6I GCGC 3 cut(s) 189, 493, 610
HinP1I GCGC 3 cut(s) 189, 493, 610
HinfI GANTC 4 cut(s) 73, 212, 233, 588
HpaII CCGG 1 cut(s) 489
Hpy166II GTNNAC 4 cut(s) 185, 319, 514, 568
Hpy188I TCNGA 4 cut(s) 10, 102, 481, 606
Hpy188III TCNNGA 5 cut(s) 49, 164, 341, 402, 585
Hpy8I GTNNAC 4 cut(s) 185, 319, 514, 568
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 4 cut(s) 185, 504, 536, 641
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 191, 364, 501, 641
HpyF3I CTNAG 6 cut(s) 69, 101, 237, 363, 384, 603
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 5 cut(s) 38, 211, 248, 420, 651
HspAI GCGC 3 cut(s) 189, 493, 610
Kzo9I GATC 1 cut(s) 5
LpnPI CCDG 9 cut(s) 44, 71, 120, 144, 159, 354, 396, 502, 598
Lsp1109I GCAGC 2 cut(s) 162, 178
LweI GCATC 2 cut(s) 475, 597
MaeII ACGT 1 cut(s) 570
MalI GATC 1 cut(s) 7
MbiI CCGCTC 2 cut(s) 42, 307
MboI GATC 1 cut(s) 5
MboII GAAGA 5 cut(s) 130, 463, 466, 551, 608
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 2 cut(s) 293, 505
MnlI CCTC 9 cut(s) 96, 372, 379, 386, 424, 536, 585, 625, 631
MseI TTAA 3 cut(s) 141, 441, 652
MspA1I CMGCKG 2 cut(s) 153, 336
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 2 cut(s) 59, 490
MvaI CCWGG 1 cut(s) 59
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 5 cut(s) 131, 191, 364, 501, 641
NciI CCSGG 1 cut(s) 490
NcoI CCATGG 1 cut(s) 207
NdeII GATC 1 cut(s) 5
NlaIII CATG 5 cut(s) 38, 211, 248, 420, 651
NlaIV GGNNCC 1 cut(s) 548
NspI RCATGY 2 cut(s) 420, 651
PaeR7I CTCGAG 1 cut(s) 402
PciI ACATGT 2 cut(s) 416, 647
PfeI GAWTC 4 cut(s) 73, 212, 233, 588
PkrI GCNGC 4 cut(s) 152, 193, 309, 643
PscI ACATGT 2 cut(s) 416, 647
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 1 cut(s) 548
PspPI GGNCC 1 cut(s) 14
PvuII CAGCTG 1 cut(s) 153
SaqAI TTAA 3 cut(s) 141, 441, 652
SatI GCNGC 4 cut(s) 151, 192, 308, 642
Sau3AI GATC 1 cut(s) 5
Sau96I GGNCC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 59, 490
SduI GDGCHC 1 cut(s) 187
SetI ASST 5 cut(s) 155, 364, 548, 573, 646
SfaNI GCATC 2 cut(s) 475, 597
SfcI CTRYAG 1 cut(s) 87
Sfr274I CTCGAG 1 cut(s) 402
SinI GGWCC 1 cut(s) 14
SlaI CTCGAG 1 cut(s) 402
SmlI CTYRAG 2 cut(s) 402, 440
SmoI CTYRAG 2 cut(s) 402, 440
Sse9I AATT 2 cut(s) 293, 505
SsiI CCGC 3 cut(s) 42, 307, 334
StyD4I CCNGG 2 cut(s) 57, 488
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 573
TaqI TCGA 2 cut(s) 403, 425
TaqII GACCGA 1 cut(s) 443
TasI AATT 2 cut(s) 293, 505
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 4 cut(s) 73, 212, 233, 588
Tru1I TTAA 3 cut(s) 141, 441, 652
Tru9I TTAA 3 cut(s) 141, 441, 652
TseI GCWGC 3 cut(s) 150, 191, 641
TspDTI ATGAA 3 cut(s) 23, 261, 609
TspGWI ACGGA 1 cut(s) 518
Vha464I CTTAAG 1 cut(s) 440
VneI GTGCAC 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 14
XagI CCTNNNNNAGG 1 cut(s) 629
XapI RAATTY 1 cut(s) 293
XceI RCATGY 2 cut(s) 420, 651
XhoI CTCGAG 1 cut(s) 402
XmiI GTMKAC 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.