Rh4CG417100

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010303
Physical Location & Seq
Reverse (-)
66210934 .. 66213471
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG417100.1

Sequence Viewer

Length: 831 bp
ATGAACAATGTGGTGATTATAATTTTGATTAAAGACCCATATGACAGACCAAACTCAGCTTTACTCTCTCTCGGTTTCTCCAACGTTCTCATTCTTCAAAGTCCACACCCAGTTGTGACCTCCGTCCTCAGTTTCAAGATTGGGAACTGTATTCAAGTGGAGAATCAGAACGAGGAAATGGGATCAGAAGGGCCAAAGAGTGTGGTGATTAATGTGACTGGATTCAAGAAGTTTCAAGGGGTGGCTGAGAATCCAACAGAGATTATAGTGAACAATCTAAAGACCTATGTGGAGAAGAGAGGGTTGCCTTCTGGTCTGACACTTGGTAGCTGCACCATTCTTGAGACTGCTGGAGATGGTGCCAGTCCAGCTCTCTACAAGGCCATGGAGTCGGGGATTTCGAAAACGGACTCTAACACCAATGATCAAGTTGTCTGGCTTCACTTGGGGGTGAATAGTGGGGCTATGAAATTTGCTATTGAGCGGCAAGCTGTAAATGAGGCCACCTTTCGGTGTCCGGATGAGTTTGGATGGCAACCTCAGCAATTCCCAATAATTGAAGCAGATGGGGAAATTTCTCGAGCAAGAGAGACTTCTTGCTCAATTGATGCAATCCTTAAGATCTTGAAGAAGAAGGGATTCGATGTGGCGGTATCAGATGATGCGGGACGCTTTGTGTGCAATTACGTCTACTATCATTCTCTCCGGTTTGCAGAAGAAAAGGGTCACAAGTCTCTATTTGTCCATGTTCCTCTCTTTTCAAGAATTGATGAAGAAACTCAGATGTGCTTTGTGGCCTCTCTTCTGGAGGCTCTTGCTGCAACATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

276

Amino Acids

30.25

Weight (kDa)

5.33

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C15 PF01470 70 - 254 1.7e-18 Pyroglutamyl peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 20
AccB1I GGYRCC 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 690
AccIII TCCGGA 1 cut(s) 517
AciI CCGC 3 cut(s) 484, 650, 665
AclI AACGTT 1 cut(s) 84
AclWI GGATC 1 cut(s) 190
AcsI RAATTY 2 cut(s) 470, 573
AfiI CCNNNNNNNGG 1 cut(s) 510
AflII CTTAAG 1 cut(s) 617
AgsI TTSAA 8 cut(s) 98, 136, 155, 226, 236, 560, 628, 762
AluBI AGCT 4 cut(s) 59, 330, 371, 491
AluI AGCT 4 cut(s) 59, 330, 371, 491
Alw26I GTCTC 3 cut(s) 338, 584, 738
AlwI GGATC 1 cut(s) 190
Ama87I CYCGRG 1 cut(s) 579
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 4 cut(s) 191, 381, 501, 795
ApeKI GCWGC 2 cut(s) 330, 818
ApoI RAATTY 2 cut(s) 470, 573
AseI ATTAAT 1 cut(s) 210
Asp700I GAANNNNTTC 1 cut(s) 638
AspS9I GGNCC 1 cut(s) 191
AsuHPI GGTGA 3 cut(s) 25, 217, 463
AsuII TTCGAA 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 579
BanI GGYRCC 1 cut(s) 359
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 2 cut(s) 317, 805
BccI CCATC 3 cut(s) 350, 525, 560
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 3 cut(s) 338, 584, 738
BfrI CTTAAG 1 cut(s) 617
BglII AGATCT 1 cut(s) 621
BisI GCNGC 3 cut(s) 331, 485, 819
BlsI GCNGC 3 cut(s) 332, 486, 820
BmeT110I CYCGRG 1 cut(s) 579
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 361
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 2 cut(s) 598, 652
BmuI ACTGGG 1 cut(s) 104
BoxI GACNNNNGTC 1 cut(s) 122
BpmI CTGGAG 2 cut(s) 372, 827
Bpu10I CCTNAGC 1 cut(s) 540
Bpu14I TTCGAA 1 cut(s) 401
BpuEI CTTGAG 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 384
BsaWI WCCGGW 2 cut(s) 517, 705
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse1I ACTGG 3 cut(s) 110, 223, 363
BseAI TCCGGA 1 cut(s) 517
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 2 cut(s) 526, 536
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMII CTCAG 5 cut(s) 69, 142, 237, 554, 794
BseNI ACTGG 3 cut(s) 110, 223, 363
BseXI GCAGC 2 cut(s) 317, 805
BsgI GTGCAG 1 cut(s) 316
BshFI GGCC 4 cut(s) 193, 383, 503, 797
BshNI GGYRCC 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 579
BsiSI CCGG 2 cut(s) 518, 706
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 3 cut(s) 338, 584, 738
BsmFI GGGAC 1 cut(s) 681
BsnI GGCC 4 cut(s) 193, 383, 503, 797
BsoBI CYCGRG 1 cut(s) 579
Bsp119I TTCGAA 1 cut(s) 401
Bsp13I TCCGGA 1 cut(s) 517
Bsp143I GATC 3 cut(s) 182, 424, 621
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 3 cut(s) 484, 650, 665
BspANI GGCC 4 cut(s) 193, 383, 503, 797
BspCNI CTCAG 5 cut(s) 68, 141, 238, 553, 793
BspEI TCCGGA 1 cut(s) 517
BspLI GGNNCC 1 cut(s) 361
BspPI GGATC 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 359
BspTI CTTAAG 1 cut(s) 617
BsrBI CCGCTC 1 cut(s) 484
BsrI ACTGG 3 cut(s) 110, 223, 363
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 3 cut(s) 182, 424, 621
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 149
Bst6I CTCTTC 2 cut(s) 290, 807
BstAFI CTTAAG 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 489
BstDEI CTNAG 5 cut(s) 55, 128, 246, 540, 780
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 526, 536
BstKTI GATC 3 cut(s) 185, 427, 624
BstMAI GTCTC 3 cut(s) 338, 584, 738
BstMBI GATC 3 cut(s) 182, 424, 621
BstMWI GCNNNNNNNGC 4 cut(s) 368, 541, 678, 818
BstNSI RCATGY 1 cut(s) 828
BstPAI GACNNNNGTC 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 317, 805
BstX2I RGATCY 1 cut(s) 621
BstYI RGATCY 1 cut(s) 621
BsuRI GGCC 4 cut(s) 193, 383, 503, 797
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 2 cut(s) 526, 536
Cac8I GCNNGC 1 cut(s) 489
Cfr13I GGNCC 1 cut(s) 191
CseI GACGC 1 cut(s) 678
CspCI CAANNNNNGTGG 2 cut(s) 183, 218
CviAII CATG 3 cut(s) 385, 746, 825
DdeI CTNAG 5 cut(s) 55, 128, 246, 540, 780
DpnI GATC 3 cut(s) 184, 426, 623
DpnII GATC 3 cut(s) 182, 424, 621
Eam1104I CTCTTC 2 cut(s) 290, 807
EarI CTCTTC 2 cut(s) 290, 807
Eco130I CCWWGG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 579
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 3 cut(s) 388, 749, 828
FaiI YATR 9 cut(s) 20, 40, 42, 266, 288, 386, 467, 747, 826
FalI AAGNNNNNCTT 2 cut(s) 577, 609
FaqI GGGAC 1 cut(s) 681
FatI CATG 3 cut(s) 384, 745, 824
FauI CCCGC 1 cut(s) 658
FauNDI CATATG 1 cut(s) 40
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 3 cut(s) 331, 485, 819
FokI GGATG 2 cut(s) 533, 543
Fsp4HI GCNGC 3 cut(s) 331, 485, 819
GluI GCNGC 3 cut(s) 331, 485, 819
GsuI CTGGAG 2 cut(s) 372, 827
HaeIII GGCC 4 cut(s) 193, 383, 503, 797
HapII CCGG 2 cut(s) 518, 706
HgaI GACGC 1 cut(s) 678
Hin1II CATG 3 cut(s) 388, 749, 828
HinfI GANTC 6 cut(s) 163, 222, 250, 389, 410, 639
HpaII CCGG 2 cut(s) 518, 706
HphI GGTGA 3 cut(s) 25, 217, 463
Hpy166II GTNNAC 3 cut(s) 104, 271, 691
Hpy188I TCNGA 5 cut(s) 168, 187, 318, 658, 783
Hpy188III TCNNGA 8 cut(s) 136, 226, 341, 518, 579, 625, 762, 806
Hpy8I GTNNAC 3 cut(s) 104, 271, 691
HpyAV CCTTC 3 cut(s) 182, 318, 628
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4IV ACGT 2 cut(s) 84, 687
HpyCH4V TGCA 5 cut(s) 333, 611, 681, 713, 821
HpyF10VI GCNNNNNNNGC 4 cut(s) 368, 541, 678, 818
HpyF3I CTNAG 5 cut(s) 55, 128, 246, 540, 780
HpySE526I ACGT 2 cut(s) 84, 687
Hsp92II CATG 3 cut(s) 388, 749, 828
Kpn2I TCCGGA 1 cut(s) 517
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 3 cut(s) 182, 424, 621
Lsp1109I GCAGC 2 cut(s) 317, 805
LweI GCATC 2 cut(s) 598, 652
MaeII ACGT 2 cut(s) 84, 687
MaeIII GTNAC 3 cut(s) 115, 214, 725
MalI GATC 3 cut(s) 184, 426, 623
MbiI CCGCTC 1 cut(s) 484
MboI GATC 3 cut(s) 182, 424, 621
MboII GAAGA 7 cut(s) 86, 307, 640, 643, 728, 785, 794
MfeI CAATTG 1 cut(s) 603
MflI RGATCY 1 cut(s) 621
MluCI AATT 8 cut(s) 21, 470, 545, 555, 573, 603, 682, 765
MlyI GAGTC 2 cut(s) 398, 404
MmeI TCCRAC 2 cut(s) 105, 278
MnlI CCTC 9 cut(s) 130, 137, 166, 293, 493, 549, 762, 802, 808
MroI TCCGGA 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 638
MseI TTAA 3 cut(s) 30, 210, 618
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 2 cut(s) 518, 706
MunI CAATTG 1 cut(s) 603
MwoI GCNNNNNNNGC 4 cut(s) 368, 541, 678, 818
NcoI CCATGG 1 cut(s) 384
NdeI CATATG 1 cut(s) 40
NdeII GATC 3 cut(s) 182, 424, 621
NlaIII CATG 3 cut(s) 388, 749, 828
NlaIV GGNNCC 1 cut(s) 361
NmuCI GTSAC 3 cut(s) 115, 214, 725
NspI RCATGY 1 cut(s) 828
NspV TTCGAA 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 579
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 4 cut(s) 163, 222, 250, 639
PkrI GCNGC 3 cut(s) 332, 486, 820
PleI GAGTC 2 cut(s) 397, 404
PpsI GAGTC 2 cut(s) 397, 404
PshAI GACNNNNGTC 1 cut(s) 122
PshBI ATTAAT 1 cut(s) 210
PsiI TTATAA 1 cut(s) 20
Psp1406I AACGTT 1 cut(s) 84
PspN4I GGNNCC 1 cut(s) 361
PspPI GGNCC 1 cut(s) 191
PsuI RGATCY 1 cut(s) 621
SaqAI TTAA 3 cut(s) 30, 210, 618
SatI GCNGC 3 cut(s) 331, 485, 819
Sau3AI GATC 3 cut(s) 182, 424, 621
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 2 cut(s) 398, 404
SfaNI GCATC 2 cut(s) 598, 652
Sfr274I CTCGAG 1 cut(s) 579
SfuI TTCGAA 1 cut(s) 401
SlaI CTCGAG 1 cut(s) 579
SmlI CTYRAG 3 cut(s) 341, 579, 617
SmoI CTYRAG 3 cut(s) 341, 579, 617
Sse9I AATT 8 cut(s) 21, 470, 545, 555, 573, 603, 682, 765
SsiI CCGC 3 cut(s) 484, 650, 665
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 1 cut(s) 149
TaiI ACGT 2 cut(s) 87, 690
TaqI TCGA 3 cut(s) 401, 580, 642
TasI AATT 8 cut(s) 21, 470, 545, 555, 573, 603, 682, 765
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 4 cut(s) 163, 222, 250, 639
Tru1I TTAA 3 cut(s) 30, 210, 618
Tru9I TTAA 3 cut(s) 30, 210, 618
TseFI GTSAC 3 cut(s) 115, 214, 725
TseI GCWGC 2 cut(s) 330, 818
Tsp45I GTSAC 3 cut(s) 115, 214, 725
TspDTI ATGAA 3 cut(s) 17, 482, 786
TspGWI ACGGA 2 cut(s) 112, 422
Vha464I CTTAAG 1 cut(s) 617
VspI ATTAAT 1 cut(s) 210
XapI RAATTY 2 cut(s) 470, 573
XceI RCATGY 1 cut(s) 828
XhoI CTCGAG 1 cut(s) 579
XmiI GTMKAC 1 cut(s) 690
XmnI GAANNNNTTC 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.