pycom16g08150

Pyroglutamyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010303
Physical Location & Seq
Reverse (-)
5385144 .. 5386937
1794 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08150.2

Sequence Viewer

Length: 654 bp
ATGGGATCAGAAGGACCAAAGAGAGTGGTGGTTCATGTGAGTGGGTTCAAGAAGTTCCAGGGGGTTGCTGAGAATCCCACAGAGAGTATAGTTAGCAATCTGGGGGCGTTTGTTGAGAAGAGAGGGTTGTCTGCTGGTCTTAAACTTGGCAACTGTGATGTTCTTGAAACAGCAGGAGACGGCGCGCGCCCCGCTCTTTACAAGGCCATGGAATCGGGGATTTCAGCAACCGGTTCTAACGGCAATGAACAAGTTGTATGGCTTCACCTGGGGGTGAATAGTGGGGCTGTAAGATTTTCTGTTGAGCGGCAAGCAGTAAATGAAGCCACTTTCCGCTGTCCAGATGAGTTAGGATGGCAACCTCAGCAACGACTAATAGTCCCTGAGGATGGAGGAATTACACGAGCAAGAGAGACATGTTGCTCGATCGAGGCAATCCTTAAGATCTTGAAGAATCGAGGATATGATGTGGCAATATCAGATGATGCTGGACGTTTCGTGTGCAATTATGTCTACTATCATTCCCTCCGTTTTGCAGAAGAAAAGGGTCACAAATCTTTATTTGTCCACGTCCCTCTCTTTTCCAGAATCGATGAAGAAACTCAGATGCGCTTTGTGGCCTCCCTCTTGGAGGCCATTGCAGCTACATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C15

Protein Analysis

218

Amino Acids

23.65

Weight (kDa)

6.23

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 194, 307
AccI GTMKAC 1 cut(s) 513
AccII CGCG 2 cut(s) 185, 187
AciI CCGC 3 cut(s) 192, 307, 334
AclWI GGATC 1 cut(s) 13
AfiI CCNNNNNNNGG 2 cut(s) 389, 631
AflII CTTAAG 1 cut(s) 440
AflIII ACRYGT 2 cut(s) 416, 647
AgeI ACCGGT 1 cut(s) 230
AgsI TTSAA 3 cut(s) 49, 167, 451
AhdI GACNNNNNGTC 1 cut(s) 377
AjiI CACGTC 1 cut(s) 571
AjnI CCWGG 2 cut(s) 57, 267
AluBI AGCT 1 cut(s) 644
AluI AGCT 1 cut(s) 644
Alw26I GTCTC 2 cut(s) 171, 407
AlwI GGATC 1 cut(s) 13
AoxI GGCC 3 cut(s) 204, 618, 633
ApeKI GCWGC 1 cut(s) 641
AsiGI ACCGGT 1 cut(s) 230
AspLEI GCGC 4 cut(s) 185, 187, 189, 612
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 257, 286
AvaII GGWCC 1 cut(s) 14
AxyI CCTNAGG 1 cut(s) 384
BauI CACGAG 1 cut(s) 402
BbvCI CCTCAGC 1 cut(s) 363
BccI CCATC 2 cut(s) 348, 383
BceAI ACGGC 2 cut(s) 196, 256
BciT130I CCWGG 2 cut(s) 59, 269
BcoDI GTCTC 2 cut(s) 171, 407
BfrI CTTAAG 1 cut(s) 440
BglII AGATCT 1 cut(s) 444
BisI GCNGC 2 cut(s) 308, 642
BlsI GCNGC 2 cut(s) 309, 643
Bme1390I CCNGG 2 cut(s) 59, 269
Bme18I GGWCC 1 cut(s) 14
BmeRI GACNNNNNGTC 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 1 cut(s) 14
BmrFI CCNGG 2 cut(s) 59, 269
BmsI GCATC 2 cut(s) 475, 597
Bpu10I CCTNAGC 1 cut(s) 363
Bsa29I ATCGAT 1 cut(s) 591
BsaJI CCNNGG 3 cut(s) 58, 207, 268
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 631
Bse118I RCCGGY 1 cut(s) 230
Bse21I CCTNAGG 1 cut(s) 384
Bse3DI GCAATG 2 cut(s) 250, 636
BseBI CCWGG 2 cut(s) 59, 269
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 3 cut(s) 58, 207, 268
BseGI GGATG 2 cut(s) 359, 394
BseLI CCNNNNNNNGG 2 cut(s) 389, 631
BseMI GCAATG 2 cut(s) 250, 636
BseMII CTCAG 4 cut(s) 60, 375, 377, 617
BsePI GCGCGC 2 cut(s) 183, 185
Bsh1236I CGCG 2 cut(s) 185, 187
Bsh1285I CGRYCG 1 cut(s) 429
BshFI GGCC 3 cut(s) 206, 620, 635
BshTI ACCGGT 1 cut(s) 230
BshVI ATCGAT 1 cut(s) 591
BsiEI CGRYCG 1 cut(s) 429
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 2 cut(s) 365, 557
BslI CCNNNNNNNGG 2 cut(s) 389, 631
BsmAI GTCTC 2 cut(s) 171, 407
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 2 cut(s) 365, 557
BsnI GGCC 3 cut(s) 206, 620, 635
Bsp143I GATC 3 cut(s) 5, 426, 444
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 3 cut(s) 192, 307, 334
BspANI GGCC 3 cut(s) 206, 620, 635
BspCNI CTCAG 4 cut(s) 61, 376, 376, 616
BspDI ATCGAT 1 cut(s) 591
BspFNI CGCG 2 cut(s) 185, 187
BspPI GGATC 1 cut(s) 13
BspTI CTTAAG 1 cut(s) 440
BsrBI CCGCTC 2 cut(s) 194, 307
BsrDI GCAATG 2 cut(s) 250, 636
BsrFI RCCGGY 1 cut(s) 230
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 3 cut(s) 58, 207, 268
BssHII GCGCGC 2 cut(s) 183, 185
BssMI GATC 3 cut(s) 5, 426, 444
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 207
Bst2BI CACGAG 1 cut(s) 402
Bst2UI CCWGG 2 cut(s) 59, 269
Bst4CI ACNGT 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 113
BstAFI CTTAAG 1 cut(s) 440
BstC8I GCNNGC 3 cut(s) 185, 187, 312
BstDEI CTNAG 4 cut(s) 69, 363, 384, 603
BstDSI CCRYGG 1 cut(s) 207
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 2 cut(s) 359, 394
BstFNI CGCG 2 cut(s) 185, 187
BstHHI GCGC 4 cut(s) 185, 187, 189, 612
BstKTI GATC 3 cut(s) 8, 429, 447
BstMAI GTCTC 2 cut(s) 171, 407
BstMBI GATC 3 cut(s) 5, 426, 444
BstMCI CGRYCG 1 cut(s) 429
BstMWI GCNNNNNNNGC 3 cut(s) 191, 364, 641
BstNI CCWGG 2 cut(s) 59, 269
BstNSI RCATGY 2 cut(s) 420, 651
BstSCI CCNGG 2 cut(s) 57, 267
BstUI CGCG 2 cut(s) 185, 187
BstX2I RGATCY 1 cut(s) 444
BstYI RGATCY 1 cut(s) 444
Bsu15I ATCGAT 1 cut(s) 591
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 3 cut(s) 206, 620, 635
BsuTUI ATCGAT 1 cut(s) 591
BtgI CCRYGG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 2 cut(s) 359, 394
Cac8I GCNNGC 3 cut(s) 185, 187, 312
CfoI GCGC 4 cut(s) 185, 187, 189, 612
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 14
ClaI ATCGAT 1 cut(s) 591
CspAI ACCGGT 1 cut(s) 230
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 4 cut(s) 35, 208, 417, 648
CviJI RGCY 7 cut(s) 206, 262, 287, 326, 620, 635, 644
CviKI_1 RGCY 7 cut(s) 206, 262, 287, 326, 620, 635, 644
DdeI CTNAG 4 cut(s) 69, 363, 384, 603
DpnI GATC 3 cut(s) 7, 428, 446
DpnII GATC 3 cut(s) 5, 426, 444
DriI GACNNNNNGTC 1 cut(s) 377
Eam1104I CTCTTC 1 cut(s) 113
Eam1105I GACNNNNNGTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 14
Eco81I CCTNAGG 1 cut(s) 384
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoRII CCWGG 2 cut(s) 57, 267
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 4 cut(s) 38, 211, 420, 651
FaiI YATR 8 cut(s) 36, 89, 209, 259, 418, 465, 510, 649
FaqI GGGAC 2 cut(s) 365, 557
FatI CATG 4 cut(s) 34, 207, 416, 647
FauI CCCGC 1 cut(s) 199
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 308, 642
FokI GGATG 2 cut(s) 366, 401
Fsp4HI GCNGC 2 cut(s) 308, 642
GlaI GCGC 4 cut(s) 184, 186, 188, 611
GluI GCNGC 2 cut(s) 308, 642
HaeIII GGCC 3 cut(s) 206, 620, 635
HapII CCGG 1 cut(s) 231
HhaI GCGC 4 cut(s) 185, 187, 189, 612
Hin1II CATG 4 cut(s) 38, 211, 420, 651
Hin6I GCGC 4 cut(s) 183, 185, 187, 610
HinP1I GCGC 4 cut(s) 183, 185, 187, 610
HinfI GANTC 4 cut(s) 73, 212, 454, 588
HpaII CCGG 1 cut(s) 231
HphI GGTGA 2 cut(s) 257, 286
Hpy166II GTNNAC 2 cut(s) 514, 568
Hpy188I TCNGA 3 cut(s) 10, 481, 606
Hpy188III TCNNGA 5 cut(s) 49, 164, 341, 448, 585
Hpy8I GTNNAC 2 cut(s) 514, 568
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 2 cut(s) 493, 570
HpyCH4V TGCA 3 cut(s) 504, 536, 641
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 364, 641
HpyF3I CTNAG 4 cut(s) 69, 363, 384, 603
HpySE526I ACGT 2 cut(s) 493, 570
Hsp92II CATG 4 cut(s) 38, 211, 420, 651
HspAI GCGC 4 cut(s) 183, 185, 187, 610
Kzo9I GATC 3 cut(s) 5, 426, 444
LweI GCATC 2 cut(s) 475, 597
MaeII ACGT 2 cut(s) 493, 570
MaeIII GTNAC 1 cut(s) 548
MalI GATC 3 cut(s) 7, 428, 446
MbiI CCGCTC 2 cut(s) 194, 307
MboI GATC 3 cut(s) 5, 426, 444
MboII GAAGA 4 cut(s) 130, 463, 551, 608
MflI RGATCY 1 cut(s) 444
MluCI AATT 2 cut(s) 396, 505
MseI TTAA 2 cut(s) 141, 441
MslI CAYNNNNRTG 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 336
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 2 cut(s) 59, 269
MvaI CCWGG 2 cut(s) 59, 269
MvnI CGCG 2 cut(s) 185, 187
MwoI GCNNNNNNNGC 3 cut(s) 191, 364, 641
NcoI CCATGG 1 cut(s) 207
NdeII GATC 3 cut(s) 5, 426, 444
NlaIII CATG 4 cut(s) 38, 211, 420, 651
NmuCI GTSAC 1 cut(s) 548
NspI RCATGY 2 cut(s) 420, 651
PauI GCGCGC 2 cut(s) 183, 185
PciI ACATGT 2 cut(s) 416, 647
PfeI GAWTC 4 cut(s) 73, 212, 454, 588
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 2 cut(s) 309, 643
Ple19I CGATCG 1 cut(s) 429
PscI ACATGT 2 cut(s) 416, 647
Psp6I CCWGG 2 cut(s) 57, 267
PspGI CCWGG 2 cut(s) 57, 267
PspPI GGNCC 1 cut(s) 14
PsuI RGATCY 1 cut(s) 444
PteI GCGCGC 2 cut(s) 183, 185
PvuI CGATCG 1 cut(s) 429
RseI CAYNNNNRTG 1 cut(s) 39
SaqAI TTAA 2 cut(s) 141, 441
SatI GCNGC 2 cut(s) 308, 642
Sau3AI GATC 3 cut(s) 5, 426, 444
Sau96I GGNCC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 59, 269
SetI ASST 5 cut(s) 270, 364, 496, 573, 646
SfaNI GCATC 2 cut(s) 475, 597
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 2 cut(s) 396, 505
SsiI CCGC 3 cut(s) 192, 307, 334
StyD4I CCNGG 2 cut(s) 57, 267
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 2 cut(s) 496, 573
TaqI TCGA 4 cut(s) 425, 429, 457, 591
TasI AATT 2 cut(s) 396, 505
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 4 cut(s) 73, 212, 454, 588
Tru1I TTAA 2 cut(s) 141, 441
Tru9I TTAA 2 cut(s) 141, 441
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 1 cut(s) 641
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 4 cut(s) 23, 261, 336, 609
TspGWI ACGGA 1 cut(s) 518
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 1 cut(s) 14
XagI CCTNNNNNAGG 1 cut(s) 629
XceI RCATGY 2 cut(s) 420, 651
XmiI GTMKAC 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.