FvH4_4g35410

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005536
Physical Location & Seq
Forward (+)
32731146 .. 32742710
11565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35410.t5

Sequence Viewer

Length: 909 bp
ATGCTACGTCAGCACCACCGTCGAGCCACCGCGACTGAGCTCACGAAAATGGCGGCGCATGTGGCAGCGCTGATCACTCACCCGCGCACGTCACCACCCTCCGCTAACGAACGCCTCTCATTCCAATCTCACCGCTCCTTCGCCACCATGACCGGAAACGACGAGTCGTCGCTCGAAACTATATTCCGGCAGAAGCACTCGCTCCGGTCCAGAATCCGCAAGGAGCTCAAGAACATGGACCCGACCCGAAAATCCGAACAAGACGCTGAAATCCAGAGCATTGTGTTGAGTGCTCCATGGTTCAAGTATAGTGAGAGCATATGTGCTTATATAAGCTCTCCTGAGTTGCGAGAAGTGGATACGGCAAGGATTGTTTCCGAAATTTTATCGAAACCCGGCAATGGTGAAGACGGTGATGTGGTGATGAGGAGGAAGCTTTATGTGCCGCGAGTTGAGGATAGGAATAGTAATATGAGAATGCTGAGGATTGACAGTGTTGATGATCTGATTGTGAAGTCTATGAATGTTTTGGAACCTGCGCTTTCAGATGCCGAGGGGAAACTATATGAAGATGGTAATGTCTGTTCATTATCTGATGATGATTTTGTTGTAGCTATGGAGGCAAAGGATCCAATTGACTTGTTCATCCTACCTGGTCTTGCTTTTGACAGATGTGGAAGACGTTTAGGCCGTAGTGGAGGTTATTATGACACGTTCCTAAAGAAGTACCAAAAGCTTGCAAAGGATCGCAAATGGAAGGATCCCCTCTGTGTGGCACTATCATATTCAATACAGATCGTGGAGGAAGGAGTCATACCTGTCACTTTGAATGATGTTTCTGTGGATGCTCTTGTATCTCCAGCTGGTTTCATTCCGATTAGCCCAGCGGCTTGGAATAGATGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

303

Amino Acids

33.95

Weight (kDa)

7.01

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 65 - 274 2.9e-26 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 544
AccBSI CCGCTC 1 cut(s) 135
AccII CGCG 3 cut(s) 32, 85, 448
AciI CCGC 8 cut(s) 30, 53, 83, 102, 133, 217, 446, 887
AclWI GGATC 5 cut(s) 623, 636, 753, 755, 768
AcsI RAATTY 1 cut(s) 381
AfaI GTAC 1 cut(s) 728
AfeI AGCGCT 1 cut(s) 69
AfiI CCNNNNNNNGG 2 cut(s) 401, 772
AflIII ACRYGT 1 cut(s) 711
AgsI TTSAA 3 cut(s) 304, 789, 829
AhdI GACNNNNNGTC 1 cut(s) 166
AjiI CACGTC 1 cut(s) 90
AjnI CCWGG 1 cut(s) 652
AluBI AGCT 7 cut(s) 40, 226, 336, 436, 614, 736, 863
AluI AGCT 7 cut(s) 40, 226, 336, 436, 614, 736, 863
Alw21I GWGCWC 3 cut(s) 42, 228, 295
AlwI GGATC 5 cut(s) 623, 636, 753, 755, 768
Aor51HI AGCGCT 1 cut(s) 69
AoxI GGCC 1 cut(s) 688
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 1 cut(s) 381
AspLEI GCGC 4 cut(s) 58, 70, 87, 541
AspS9I GGNCC 2 cut(s) 207, 238
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 6 cut(s) 71, 84, 122, 416, 425, 433
AvaII GGWCC 2 cut(s) 207, 238
BamHI GGATCC 2 cut(s) 628, 760
BanII GRGCYC 2 cut(s) 42, 228
BarI GAAGNNNNNNTAC 2 cut(s) 798, 830
BbsI GAAGAC 2 cut(s) 414, 685
Bbv12I GWGCWC 3 cut(s) 42, 228, 295
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 1 cut(s) 77
BccI CCATC 1 cut(s) 566
BceAI ACGGC 2 cut(s) 378, 675
BcgI CGANNNNNNTGC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 654
BciVI GTATCC 1 cut(s) 352
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 396
BfoI RGCGCY 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 544
BfuI GTATCC 1 cut(s) 352
BisI GCNGC 4 cut(s) 54, 66, 446, 888
BlsI GCNGC 4 cut(s) 55, 67, 447, 889
Bme1390I CCNGG 2 cut(s) 396, 654
Bme18I GGWCC 2 cut(s) 207, 238
BmeRI GACNNNNNGTC 1 cut(s) 166
BmgBI CACGTC 1 cut(s) 90
BmgT120I GGNCC 2 cut(s) 207, 238
BmiI GGNNCC 4 cut(s) 240, 534, 630, 762
BmrFI CCNGG 2 cut(s) 396, 654
BmsI GCATC 2 cut(s) 538, 835
BpiI GAAGAC 2 cut(s) 414, 685
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 396
BsaJI CCNNGG 2 cut(s) 296, 552
BsaWI WCCGGW 2 cut(s) 152, 204
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 772
Bse3DI GCAATG 1 cut(s) 406
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 2 cut(s) 296, 552
BseGI GGATG 2 cut(s) 645, 850
BseLI CCNNNNNNNGG 2 cut(s) 401, 772
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 3 cut(s) 27, 333, 473
BseRI GAGGAG 1 cut(s) 442
BseXI GCAGC 1 cut(s) 77
BseYI CCCAGC 1 cut(s) 883
Bsh1236I CGCG 3 cut(s) 32, 85, 448
BshFI GGCC 1 cut(s) 690
BsiHKAI GWGCWC 3 cut(s) 42, 228, 295
BsiSI CCGG 4 cut(s) 153, 187, 205, 396
BslI CCNNNNNNNGG 2 cut(s) 401, 772
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 1 cut(s) 690
Bsp1286I GDGCHC 3 cut(s) 42, 228, 295
Bsp143I GATC 6 cut(s) 72, 502, 628, 745, 760, 795
Bsp19I CCATGG 1 cut(s) 296
BspACI CCGC 8 cut(s) 30, 53, 83, 102, 133, 217, 446, 887
BspANI GGCC 1 cut(s) 690
BspCNI CTCAG 3 cut(s) 28, 334, 474
BspFNI CGCG 3 cut(s) 32, 85, 448
BspLI GGNNCC 4 cut(s) 240, 534, 630, 762
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 5 cut(s) 623, 636, 753, 755, 768
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 406
BssECI CCNNGG 2 cut(s) 296, 552
BssMI GATC 6 cut(s) 72, 502, 628, 745, 760, 795
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 3 cut(s) 20, 413, 494
BstC8I GCNNGC 1 cut(s) 738
BstDEI CTNAG 3 cut(s) 36, 342, 482
BstDSI CCRYGG 1 cut(s) 296
BstF5I GGATG 2 cut(s) 645, 850
BstFNI CGCG 3 cut(s) 32, 85, 448
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 4 cut(s) 58, 70, 87, 541
BstKTI GATC 6 cut(s) 75, 505, 631, 748, 763, 798
BstMBI GATC 6 cut(s) 72, 502, 628, 745, 760, 795
BstMWI GCNNNNNNNGC 4 cut(s) 10, 62, 442, 620
BstNI CCWGG 1 cut(s) 654
BstNSI RCATGY 1 cut(s) 62
BstSCI CCNGG 2 cut(s) 394, 652
BstUI CGCG 3 cut(s) 32, 85, 448
BstV1I GCAGC 1 cut(s) 77
BstV2I GAAGAC 2 cut(s) 414, 685
BstX2I RGATCY 2 cut(s) 628, 760
BstXI CCANNNNNNTGG 1 cut(s) 891
BstYI RGATCY 2 cut(s) 628, 760
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 1 cut(s) 690
BtgI CCRYGG 1 cut(s) 296
BtrI CACGTC 1 cut(s) 90
BtsCI GGATG 2 cut(s) 645, 850
BtsIMutI CAGTG 1 cut(s) 499
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 1 cut(s) 738
CfoI GCGC 4 cut(s) 58, 70, 87, 541
Cfr13I GGNCC 2 cut(s) 207, 238
CseI GACGC 1 cut(s) 272
CsiI ACCWGGT 1 cut(s) 652
Csp6I GTAC 1 cut(s) 727
CviAII CATG 4 cut(s) 59, 148, 235, 297
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 3 cut(s) 36, 342, 482
DpnI GATC 6 cut(s) 74, 504, 630, 747, 762, 797
DpnII GATC 6 cut(s) 72, 502, 628, 745, 760, 795
DriI GACNNNNNGTC 1 cut(s) 166
Eam1105I GACNNNNNGTC 1 cut(s) 166
Ecl136II GAGCTC 2 cut(s) 40, 226
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 42, 228
Eco47I GGWCC 2 cut(s) 207, 238
Eco47III AGCGCT 1 cut(s) 69
Eco53kI GAGCTC 2 cut(s) 40, 226
EcoICRI GAGCTC 2 cut(s) 40, 226
EcoRII CCWGG 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 42, 228
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 4 cut(s) 62, 151, 238, 300
FatI CATG 4 cut(s) 58, 147, 234, 296
FauI CCCGC 1 cut(s) 90
FauNDI CATATG 1 cut(s) 320
FbaI TGATCA 1 cut(s) 72
Fnu4HI GCNGC 4 cut(s) 54, 66, 446, 888
FokI GGATG 2 cut(s) 632, 857
FriOI GRGCYC 2 cut(s) 42, 228
Fsp4HI GCNGC 4 cut(s) 54, 66, 446, 888
GlaI GCGC 4 cut(s) 57, 69, 86, 540
GluI GCNGC 4 cut(s) 54, 66, 446, 888
GsaI CCCAGC 1 cut(s) 887
GsuI CTGGAG 1 cut(s) 843
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 1 cut(s) 690
HapII CCGG 4 cut(s) 153, 187, 205, 396
HgaI GACGC 1 cut(s) 272
HhaI GCGC 4 cut(s) 58, 70, 87, 541
Hin1II CATG 4 cut(s) 62, 151, 238, 300
Hin6I GCGC 4 cut(s) 56, 68, 85, 539
HinP1I GCGC 4 cut(s) 56, 68, 85, 539
HindIII AAGCTT 2 cut(s) 434, 734
HinfI GANTC 3 cut(s) 164, 213, 810
HpaII CCGG 4 cut(s) 153, 187, 205, 396
HphI GGTGA 6 cut(s) 71, 84, 122, 416, 425, 433
Hpy188I TCNGA 6 cut(s) 256, 379, 507, 547, 595, 876
Hpy188III TCNNGA 5 cut(s) 43, 210, 229, 274, 341
Hpy99I CGWCG 3 cut(s) 24, 164, 172
HpyAV CCTTC 3 cut(s) 148, 751, 800
HpyCH4III ACNGT 3 cut(s) 20, 413, 494
HpyCH4IV ACGT 4 cut(s) 7, 89, 682, 713
HpyCH4V TGCA 1 cut(s) 740
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 62, 442, 620
HpyF3I CTNAG 3 cut(s) 36, 342, 482
HpySE526I ACGT 4 cut(s) 7, 89, 682, 713
Hsp92II CATG 4 cut(s) 62, 151, 238, 300
HspAI GCGC 4 cut(s) 56, 68, 85, 539
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 6 cut(s) 72, 502, 628, 745, 760, 795
LmnI GCTCC 4 cut(s) 140, 207, 223, 298
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 2 cut(s) 538, 835
MabI ACCWGGT 1 cut(s) 652
MaeII ACGT 4 cut(s) 7, 89, 682, 713
MaeIII GTNAC 2 cut(s) 90, 820
MalI GATC 6 cut(s) 74, 504, 630, 747, 762, 797
MbiI CCGCTC 1 cut(s) 135
MboI GATC 6 cut(s) 72, 502, 628, 745, 760, 795
MboII GAAGA 3 cut(s) 419, 581, 690
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 2 cut(s) 628, 760
MhlI GDGCHC 3 cut(s) 42, 228, 295
MluCI AATT 2 cut(s) 381, 633
MlyI GAGTC 2 cut(s) 173, 819
MslI CAYNNNNRTG 1 cut(s) 47
MspA1I CMGCKG 2 cut(s) 863, 887
MspI CCGG 4 cut(s) 153, 187, 205, 396
MspR9I CCNGG 2 cut(s) 396, 654
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 3 cut(s) 32, 85, 448
MwoI GCNNNNNNNGC 4 cut(s) 10, 62, 442, 620
NciI CCSGG 1 cut(s) 396
NcoI CCATGG 1 cut(s) 296
NdeI CATATG 1 cut(s) 320
NdeII GATC 6 cut(s) 72, 502, 628, 745, 760, 795
NlaIII CATG 4 cut(s) 62, 151, 238, 300
NlaIV GGNNCC 4 cut(s) 240, 534, 630, 762
NmeAIII GCCGAG 1 cut(s) 577
NmuCI GTSAC 2 cut(s) 90, 820
NspI RCATGY 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PctI GAATGC 1 cut(s) 483
PfeI GAWTC 1 cut(s) 213
PkrI GCNGC 4 cut(s) 55, 67, 447, 889
PleI GAGTC 2 cut(s) 172, 818
PpsI GAGTC 2 cut(s) 172, 818
Psp124BI GAGCTC 2 cut(s) 42, 228
Psp6I CCWGG 1 cut(s) 652
PspFI CCCAGC 1 cut(s) 883
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 4 cut(s) 240, 534, 630, 762
PspPI GGNCC 2 cut(s) 207, 238
PsrI GAACNNNNNNTAC 2 cut(s) 568, 600
PsuI RGATCY 2 cut(s) 628, 760
PvuII CAGCTG 1 cut(s) 863
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 2 cut(s) 42, 228
SatI GCNGC 4 cut(s) 54, 66, 446, 888
Sau3AI GATC 6 cut(s) 72, 502, 628, 745, 760, 795
Sau96I GGNCC 2 cut(s) 207, 238
SchI GAGTC 2 cut(s) 173, 819
ScrFI CCNGG 2 cut(s) 396, 654
SduI GDGCHC 3 cut(s) 42, 228, 295
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 2 cut(s) 538, 835
SinI GGWCC 2 cut(s) 207, 238
SmiMI CAYNNNNRTG 1 cut(s) 47
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 381, 633
SsiI CCGC 8 cut(s) 30, 53, 83, 102, 133, 217, 446, 887
SstI GAGCTC 2 cut(s) 42, 228
StyD4I CCNGG 2 cut(s) 394, 652
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 3 cut(s) 20, 413, 494
TaiI ACGT 4 cut(s) 10, 92, 685, 716
TaqI TCGA 3 cut(s) 22, 174, 389
TasI AATT 2 cut(s) 381, 633
TauI GCSGC 3 cut(s) 56, 448, 890
TfiI GAWTC 1 cut(s) 213
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 2 cut(s) 90, 820
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 2 cut(s) 90, 820
TspDTI ATGAA 5 cut(s) 536, 576, 582, 634, 859
TspRI CASTG 1 cut(s) 499
VpaK11BI GGWCC 2 cut(s) 207, 238
XapI RAATTY 1 cut(s) 381
XceI RCATGY 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.